EP0346732A1 - Biologische Sammel- und Transportvorrichtung - Google Patents

Biologische Sammel- und Transportvorrichtung Download PDF

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Publication number
EP0346732A1
EP0346732A1 EP89110206A EP89110206A EP0346732A1 EP 0346732 A1 EP0346732 A1 EP 0346732A1 EP 89110206 A EP89110206 A EP 89110206A EP 89110206 A EP89110206 A EP 89110206A EP 0346732 A1 EP0346732 A1 EP 0346732A1
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EP
European Patent Office
Prior art keywords
swab
atcc
polyurethane
biological sample
swabs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP89110206A
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English (en)
French (fr)
Other versions
EP0346732B1 (de
Inventor
James F. Monthony
David T. Stitt
C. Michael Gosnell
Shannon D. Stewart
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
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Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Priority to AT89110206T priority Critical patent/ATE83371T1/de
Publication of EP0346732A1 publication Critical patent/EP0346732A1/de
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Publication of EP0346732B1 publication Critical patent/EP0346732B1/de
Expired legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
    • A61B10/02—Instruments for taking cell samples or for biopsy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F13/00—Bandages or dressings; Absorbent pads
    • A61F13/15—Absorbent pads, e.g. sanitary towels, swabs or tampons for external or internal application to the body; Supporting or fastening means therefor; Tampon applicators
    • A61F13/38—Swabs having a stick-type handle, e.g. cotton tips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/22—Means for packing or storing viable microorganisms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
    • A61B10/0096—Casings for storing test samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N2001/028—Sampling from a surface, swabbing, vaporising

Definitions

  • the present invention relates to devices for collecting and transporting biological specimens. More particularly, it relates to an improved swab for collecting samples and a simplified transport device incorporating the improved swab.
  • Detecting the presence of pathogenic microbial species requires, as a first step, collection of an appropriate sample.
  • a sterile collection device such as a swab is used.
  • these swabs have been made of various materials such as cotton, sheep wool, polyester and rayon.
  • the identification method may be conventional culturing followed by identification or immunometric assay.
  • a persistent problem for samples to be cultured has been maintaining viability of pathogenic organisms.
  • a persistent problem has been recovery of immunologicaly active material.
  • the sample needs to be protected from contamination by the environment during transport.
  • Numerous devices have been devised that provide both a means for obtaining the sample and means for protecting the sample during transport. Most often these devices comprise a swabbing element having a shaft typically of wood or plastic and a swabbing tip which has universally been produced from fibrous material such as cotton fibers, wool, polyester fibers or rayon fibers.
  • the medium is often retained inside the specimen collection device and adjacent to the specimen collection swabbing tip by an absorbent fibrous swatch of material.
  • This swatch or pledget can be a woven or non-woven section of fabric or a piece of fibrous material such as cotton or rayon. While serving to restrain the flow of the aqueous media and prevent dehydration of the collected sample, the pledget materials such as cotton, polyester or rayon currently utilized do not enhance and may possibly be detrimental to preserving the viability of the microorganisms collected.
  • Certain devices have eliminated the need for a liquid retaining pledget by substituting an agar containing medium for the liquid medium.
  • the agar produces a gelled or highly viscous medium into which the specimen swab is placed after collecting the sample.
  • the agar medium provides protection, but leads to agar residue on the swab. This residue can subsequently interfere with analytical procedures such as specimen staining for visual microscopic detection of organisms.
  • Agar has also been found to interfere with certain latex agglutination tests commonly employed. Stuart et al. (cited above) have shown agar to be toxic to certain organisms.
  • Devices having a liquid transport medium are expensive to make because they have multiple elements which must be formulated or made and then assembled.
  • the devices with agar transport medium are less than acceptable because the agar interferes with subsequent testing.
  • Polyurethane has been used in other health care applications.
  • one brand of contraceptive sponge is made from a special grade of polyurethane foam made from a foamable hydrophilic prepolymer resin available from W. R. Grace & Co. and sold with the trademark Hypol. These resins are derived from toluene diisocyanate and methylenediphenyl diisocyanate. They have also been used in wound dressings. The polymers made from these resins are said to have no extractable toluene diamine, toluene diisocyanate, or other primary aromatic amines.
  • Polyurethane is recommended for use in a unitary molded swab described in U. S. Pat. No. 3,871,375. That patent states that the swab may be used for "application of medication, the removal of earwax, and all of the other uses for which swabs are normally employed.” Col. 2, lines 16-18. It also states that the swab may be sterilized. It does not suggest use for collecting biological specimens and therefore does not address the known toxicity of polyurethane to microorganisms.
  • polyurethane foam has been reported to be useful as a swab tip for removing foreign materials from a surface and to apply fluids such as paint, cosmetics, and medicines.
  • U. S. Pat. No. 3,724,018 describes a swab made with a reticulated plastic foam material, such as polyurethane foam, wrapped around an end of a stick. The patent does not address sterilizing the swab or the known toxicity of polyurethane to microorganisms.
  • swabbing material a polyurethane foam which is non-toxic as demonstrated by a lack of a zone of growth inhibition when placed on a semi-solid growth medium smeared with a suspension of N. meningitidis (Quality Control Collection, Becton Dickinson Microbiology Systems, ATCC 53900), N. gonorrhoeae (ATCC 19424) or N. gonorrhoeae (ATCC 43070) and which has open cells at its exposed surface.
  • the new swab is comprised of a shaft and a sterile swabbing tip secured to one end of the shaft.
  • the swabbing tip is formed with a polyurethane foam which is non-toxic as demonstrated by a lack of a zone of growth inhibition when placed on a semi-solid medium smeared with a suspension of N. meningitidis (Quality Control Collection, Becton Dickinson Microbiology Systems, ATCC 53900), N. gonorrhoeae (ATCC 19424) Or N. gonorrhoeae (ATCC 43070) and which has open cells at its exposed surface.
  • the new swab can be used in a collection and transport device that is much simpler than existing devices.
  • the new collection and transport device of the present invention comprises the new swab together with a cap secured to the end of the shaft opposite the swabbing tip and a tubular swab cover which covers the swab and mates with the cap to protect the swab from the environment.
  • the collection and transport device may be free of any transport medium.
  • a sample inoculator is provided.
  • the sample inoculator is secured to the exterior of the cap and a inoculator cover is provided to protect the inoculator from contamination prior to use.
  • the swab 9 of the present invention has a shaft 10 and a swabbing tip 11.
  • the swab is secured to a cap 12 that mates with a swab cover 13 to form a slidable seal.
  • An optional sample inoculator 15 is attached to cap 12 and protected by inoculator cover 14. While the inoculator is shown in an elliptical shape, those skilled in the art will appreciate that a variety of geometries can be used. For example, the inoculator could be formed as a cube or a tetrahedron.
  • the transport and collection device of the present invention does not require a liquid or solid transport medium. Elimination of these elements makes manufacture and assembly of the device much easier and therefore less expensive than manufacture and assembly of presently existing devices.
  • the preferred foams used in the present invention can be sterilized with autoclaves opening up the possibility of using this sterilization procedure if appropriate materials are used for the remaining components of the transport device.
  • the addition of sample inoculator 15 and inoculator cover 14 further enhances the usefulness of the device.
  • shaft 10 for example wood, plastic or wire might be utilized.
  • cap 12, swab cover 13, and sample inoculator 15 can be made of materials well known to those skilled in the art.
  • the advance of the present invention is use for the swabbing tip 11 of a sterile polyurethane foam which is non-toxic as demonstrated by a lack of a zone of growth inhibition when placed on a semi-solid growth medium smeared with a suspension of N. meningitidis (Quality Control Collection, Becton Dickinson Microbiology Systems, ATCC 53900), N. gonorrhoeae (ATCC 19424) or N. gonorrhoeae (ATCC 43070) and which has open cells on its exposed surface.
  • N. meningitidis Quality Control Collection, Becton Dickinson Microbiology Systems, ATCC 53900
  • N. gonorrhoeae ATCC 19424
  • N. gonorrhoeae ATCC 43070
  • Swabs which are found to be non-toxic to these three organisms after incubations on suitable semi-solid growth media for twenty-four, twenty-four and forty eight hours respectively have been found to be non-toxic to a wide variety of human pathogens that are cultured from specimens collected with swabs and transported to the microbiology laboratory under conditions typically encountered by microbiologists. They have also been found to provide substantial improvement in recovery of materials that will participate in specific binding reactions in immunoassays.
  • the swabbing tip has open cells at its exposed surface which in use contacts the sampling site to collect the sample.
  • the open cells at the surface may be achieved by using a reticulated foam or by using a non-reticulated foam and shearing the cells at the surface to create open cells.
  • the foam has 20 to 200 pores per inch (8 to 80 pores per cm) at the surface and the cells fall within a size range of 0.0196 mm to 0.196 mm average diameter. Most preferably cells having an average diameter greater than 0.2 mm should be avoided. Shearing of a non-reticulated foam to create open cells at the surface may conveniently occur in a fabrication step whereby large blocks of foam are cut to size for attachment to the shaft.
  • the swabbing tip 11 may have an inner core 16 to facilitate attachment and manufacture or may be attached directly to shaft 10.
  • the foam should be a medical grade foam substant ially free of leachable monomers that can be toxic to microorganisms. Particularly preferred are foams sold under trade designations "SCOTFOAM Custom Foam”, “SCOTFOAM Custom Foam CL”, and “SCOTFOAM Special Pore-Custom Foam” having 60 to 100 pores per inch (24 to 40 pores per cm) in either pigmented or unpigmented form (all from SCOTFOAM Corp., Eddystone, PA).
  • the unexpected and unique ability of the polyurethane foam to maintain viable microorganisms without an aqueous medium and a pledget is most unexpected.
  • nothing in the prior art of microbio logical swabs or polyurethane foams suggests that the polyurethane foam will provide a device that maintains the viability of microorganisms for periods equal or greater to the periods for which a medium wetted fiber swab can maintain such organism viability.
  • the sterile swabbing tip made with a non-toxic polyurethane foam having open cells at its exposed surface and the simplified collection and transport device of the present invention were compared to commercially available products.
  • Fastidious organisms used were Haemophilus influenzae , Neisseria meningit- idis and Neisseria gonorrhoeae .
  • the non-fastidious organisms Streptococcus pyogenes (Group A Strep) and Streptococcus pneumoniae were also studied.
  • Two different strains of each fastidious and non-fastidious organisms, A and B, were studied. Table I identifies the actual strains and their sources. These organisms are all common potential human pathogens and are typical of the type of organism which may be sampled from a patient with a swab.
  • the bacteria were grown in a broth culture medium. Then a suspension of each organism was diluted and its turbidity was measured in a spectrophotometer. The technique of quantitative plate counts was utilized to construct a graph relating the measured optical density to the number of organisms present. Thereafter, the number of organisms in a suspension was estimated by measurement of the optical density of the suspension and reading the concentration from the corresponding graph.
  • Suspensions were produced that contained approximately 5 X 107 colony forming units (CFU) per milliliter (ml). Each swab tested was inoculated by placing a 0.1 ml aliquot of the standard suspension in a sterile test tube, inserting the swab and allowing the aliquot to absorb into the swabbing tip.
  • CFU colony forming units
  • swabs were from commercially available products, a rayon swab provided with the CulturetteTM Collection and Transport System (Marion Scientific, Kansas City, MO) and a mini-size bonded polyurethane foam tip swab commonly sold for cleaning electronic surfaces (The Texwipe Co., Upper Saddle River, NJ catalog no. TX710). These polyurethane swabs are made with a non-reticulated foam which has open cells at its exposed surface. The rayon swabs were provided sterile. The polyurethane swabs were sterilized by gamma radiation prior to use.
  • the inoculated rayon tipped swabs were returned to the transport tube and activated in accordance with the manufacturer's directions to bring the medium into contact with the pledget and swabbing tip.
  • the inoculated polyurethane foam tipped swabs were placed individually in sterile screw-capped plastic tubes. Multiple swabs were inoculated for streaking at the various time intervals.
  • Replicate samples of each type swab were stored aerobically at ambient temperatures. At timed intervals of 0, 4, 8, 24 or 48 hours, depending on the organism under study, the swab was used to inoculate a petri plate of an appropriate nutritive agar medium. Each inoculated plate was systematically streaked with a bacteriological loop according to the semi-quantita tive "four quadrant method" commonly practiced by those skilled in the art of microbiology. Specifically, the plate was inoculated by:
  • the sterile swabbing tip made with a non-toxic polyurethane foam having open cells at its exposed surface and the simplified collection and transport device of the present invention were compared to another commercially available sterile swabbing tip.
  • the BBL Port-A-CulTM Aerobic Transport Device (Becton Dickinson Microbiology Systems, Cockeysville, MD), has a rayon tipped swab, a rayon pledget and a medium following the formulation of Amies. The devices are sterilized by gamma radiation. After inoculation of the rayon swabs as in Example 1, the swabs were returned to the Port-A-CulTM device and activated according to the manufacturer's directions.
  • Example 1 The polyurethane swabs used and their method of inoculation and storage were as in Example 1. These tests were conducted with a similar panel of organisms as was used in Example 1. The results are shown in Table III (average score for duplicate runs). Here again a pattern of improved organism recovery through use of the polyurethane swabs over that obtained from the rayon swabs was observed. For some organisms, the use of the polyurethane swabs allowed recovery at time periods where the rayon swabs showed no growth.
  • the sterile swabbing tip made with a non toxic polyurethane foam having open cells at its exposed surface and the simplified collection and transport device of the present invention were compared again to a commercially available swabbing tip.
  • the procedure followed and the commercially available rayon tip were identical to those of Example 2.
  • the organisms were different.
  • An additional 11 bacterial species and 1 yeast species were tested.
  • the organisms are all potential human pathogens which can be collected from a patient using a swab collection and transport device.
  • Table IV average score for duplicate runs). With seven of eleven organisms studied, recovery from the polyurethane swab was equal.
  • Example 1 the sterile swabbing tips made with a non toxic polyurethane foam having open cells at its exposed surface which were studied received no additional media after inoculation and during storage.
  • the rayon tipped swabs were moistened after activation in accordance with the instructions of their respective manufacturers.
  • all of the swabs were stored in dry tubes.
  • the polyurethane swabs were the same as those used in Examples 1, 2, and 3.
  • Rayon tipped swabs were obtained from BBL Port-A-CulTM devices (Becton Dickinson Microbiology Systems, Cockeysville, MD) and from CulturetteTM devices (Marion Scientific, Kansas City, MO).
  • enhanced recovery observed with the polyurethane swabs is related to type swab material and not method of storage or sterilization.
  • Bacteria used in this experiment are as identified in Table I, and media used for growth and toxicity testing are as listed in Table VI.
  • Each strain was grown overnight on the appropriate medium at 37° C in an atmosphere enriched with 5% carbon dioxide.
  • a suspension of each organism was then prepared in saline that contained approximately 1.5 X 108 cfu/ml.
  • the surface of the toxicity testing medium indicated in Table 6 was systematically inoculated by a cross-streaked method, commonly practiced by those skilled in the art of microbiology, so as to produce confluent growth over the surface of the medium after incubation at 37° C in an atmosphere enriched with 5% carbon dioxide.
  • swab tip materials were aseptically placed on the inoculated medium surface and plates were incubated as described above.
  • Rayon tipped swabs were obtained from CulturetteTMdevices (Marion Scientific, Kansas City, MO) and BBL Port-A-CulTM devices (Becton Dickinson Microbiology Systems, Cockeysville, MD). Polyurethane tipped swabs were obtained from two sources: The Texwipe Co., Upper Saddle River, NJ (Catalog No. TX710) and Wilshire Foam Products, Inc., Carson City, CA (Catalog Nos. HT1001 and HT1005). The rayon swabs were provided sterile. The Texwipe and Wilshire HT1001 polyurethane swabs were sterilized with gamma radiation. The Wilshire HT1005 was asceptically used as supplied.
  • Group A Streptococcal antigen was tested.
  • Group A Streptococcus Streptococcus pyogenes , ATCC 12385
  • a suspension was then prepared in saline and the turbidity was adjusted with spectrophotometer to obtain approximately 1 X 109 colony forming units (CFU) per milliliter (ml). Additional dilutions of this stock suspension were prepared in saline so as to contain in 0.050 ml 50 X 105, 12.5 X 105, 3.0 X 105, 1.5 X 105 and 0.75 X 105 CFU.
  • a series of swabs were then inoculated by placing a 0.050 ml aliqout of suspension in a sterile test tube, inserting the swab and allowing the aliquot to absorb into the swabbing tip.
  • swabs were also placed in tubes containing only 0.050 ml of saline before extraction. All swabs were tested in duplicate. Each set of duplicate swabs as well as the control swabs were assayed directly for Group A streptococcal antigen using the Directigen 1-2-3TM Group A liposome immunoassay (Becton Dickinson Microbiology Systems, Cockeysville, MD; cat. no. 8525-40).
  • a dacron swab supplied with the Directigen 1-2-3TM assay kit, the polyurethane swab available described in Example 1, and the polyurethane swab described in Example 5 (Wilshire Contamination Control, Carson, CA; catalog no. 1001). Also tested was an experimental swab manufactured by Wilshire Contamination Control using ScotFoam Special Pore-Custom FoamTM having 100 pores per inch (40 pores per cm) and white pigment. Each swab had an over all length of 6.0 in. (15.24 cm) and was comprised of a swabbing tip measuring about 0.625 in (1.59 cm) in length and 0.188 in. (0.48 cm) in diameter secured to a white polystyrene shaft of 0.094 in (0.238 cm) diameter. All polyurethane swabs were sterilized with gamma irradiation prior to use.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Veterinary Medicine (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Public Health (AREA)
  • Biochemistry (AREA)
  • Vascular Medicine (AREA)
  • Epidemiology (AREA)
  • Pathology (AREA)
  • Medical Informatics (AREA)
  • Surgery (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP89110206A 1988-06-09 1989-06-06 Biologische Sammel- und Transportvorrichtung Expired EP0346732B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT89110206T ATE83371T1 (de) 1988-06-09 1989-06-06 Biologische sammel- und transportvorrichtung.

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US20443188A 1988-06-09 1988-06-09
US204431 1988-06-09
US34614289A 1989-05-04 1989-05-04
US346142 1989-05-04

Publications (2)

Publication Number Publication Date
EP0346732A1 true EP0346732A1 (de) 1989-12-20
EP0346732B1 EP0346732B1 (de) 1992-12-16

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ID=26899476

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Application Number Title Priority Date Filing Date
EP89110206A Expired EP0346732B1 (de) 1988-06-09 1989-06-06 Biologische Sammel- und Transportvorrichtung

Country Status (8)

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EP (1) EP0346732B1 (de)
JP (1) JP2584513B2 (de)
AU (1) AU623893B2 (de)
CA (1) CA1340208C (de)
DE (1) DE68903885T2 (de)
DK (1) DK170408B1 (de)
FI (1) FI892832L (de)
MY (1) MY106081A (de)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0940678A1 (de) * 1998-03-02 1999-09-08 Akzo Nobel N.V. Analytisches Testgerät
CN108036994A (zh) * 2017-11-29 2018-05-15 爱威科技股份有限公司 一种取样装置、其使用方法及制取样本的设备
CN109628286A (zh) * 2019-01-15 2019-04-16 泰普生物科学（中国）有限公司 一种运输培养装置
CN111134732A (zh) * 2020-01-15 2020-05-12 高婷婷 宫颈粘液拭子

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2044422C (en) * 1990-07-10 1995-02-07 Hans-Joachim Burkardt Transport system for conveying biological samples
JP4672855B2 (ja) * 2000-11-10 2011-04-20 栄研化学株式会社 採便容器
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
US8728414B2 (en) 2009-05-14 2014-05-20 Dna Genotek Inc. Closure, containing apparatus, and method of using same
IT1401447B1 (it) 2010-06-09 2013-07-26 Copan Italia Spa Metodo per il trasferimento quantitativo di analiti
IT1403618B1 (it) 2011-01-05 2013-10-31 Copan Italia Spa Procedimento per realizzare un dispositivo per il prelievo ed il trasferimento di campioni per biologia molecolare
KR20140040239A (ko) 2011-06-19 2014-04-02 아보젠, 인크. 샘플 수집을 위한 장치, 용액 및 방법
JP2013079896A (ja) * 2011-10-05 2013-05-02 Kyoei Giken Kk 検体採取具用鞘管

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GB1332947A (en) * 1970-07-20 1973-10-10 Curtis W H Cytological sampling device
FR2190480A1 (en) * 1972-06-27 1974-02-01 Lhd Lab Hygiene Dietetique Rods with terminal absorbent foamed plastic pads - for hygienic, cleaning or medical uses
US4150950A (en) * 1977-09-28 1979-04-24 Corning Glass Works Transport system for clinical specimens

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JPS451036Y1 (de) * 1966-05-16 1970-01-17
DE3220139A1 (de) * 1982-05-28 1983-12-01 Merck Patent Gmbh, 6100 Darmstadt Sammel- und transportvorrichtung fuer mikroorganismen

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1332947A (en) * 1970-07-20 1973-10-10 Curtis W H Cytological sampling device
FR2190480A1 (en) * 1972-06-27 1974-02-01 Lhd Lab Hygiene Dietetique Rods with terminal absorbent foamed plastic pads - for hygienic, cleaning or medical uses
US4150950A (en) * 1977-09-28 1979-04-24 Corning Glass Works Transport system for clinical specimens

Non-Patent Citations (1)

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Title
APPLIED MICROBIOLOGY, vol. 29, no. 5, May 1975, pages 615-620, American Society for Microbiology, US; J.A. BACH et al.: "Inhibition of microbial growth by fatty amine catalysts from polyurethane foam test tube plugs" *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0940678A1 (de) * 1998-03-02 1999-09-08 Akzo Nobel N.V. Analytisches Testgerät
CN108036994A (zh) * 2017-11-29 2018-05-15 爱威科技股份有限公司 一种取样装置、其使用方法及制取样本的设备
CN109628286A (zh) * 2019-01-15 2019-04-16 泰普生物科学（中国）有限公司 一种运输培养装置
CN109628286B (zh) * 2019-01-15 2022-01-07 泰普生物科学（中国）有限公司 一种运输培养装置
CN111134732A (zh) * 2020-01-15 2020-05-12 高婷婷 宫颈粘液拭子

Also Published As

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FI892832A7 (fi) 1989-12-10
DE68903885D1 (de) 1993-01-28
AU3596289A (en) 1989-12-14
CA1340208C (en) 1998-12-15
MY106081A (en) 1995-03-31
DE68903885T2 (de) 1993-04-22
EP0346732B1 (de) 1992-12-16
DK281389A (da) 1989-12-10
AU623893B2 (en) 1992-05-28
DK281389D0 (da) 1989-06-08
JP2584513B2 (ja) 1997-02-26
FI892832A0 (fi) 1989-06-08
DK170408B1 (da) 1995-08-21
JPH0242972A (ja) 1990-02-13
FI892832L (fi) 1989-12-10

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