Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
  • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
  • G01N33/531—Production of immunochemical test materials
  • G01N33/532—Production of labelled immunochemicals
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
  • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies

Definitions

• Monoclonal antibodies to naturally occurring binding proteins which fall within the scope of this invention include but are not limited to the following functionalities: 1) antibodies which recognize binding protein and do not interfere with binding of ligand by the binding protein; and
• the preferred class of antibodies which bind to binding protein irrespective of whether ligand is bound and which do not interfere with subsequent binding of ligand antibodies, are expected to produce a positive result in the RISA as well as in the direct and indirect ELISA assays.
• Antibodies presumed to bind binding protein only when ligand is not bound would be expected to be positive in the direct ELISA and RISA and would be expected to be negative in the indirect ELISA. Based upon these primary screening results antibodies can be further characterized as to their abilities to react with binding protein under the various conditions of analyte binding.
• FBP anti-FBP-AP complex was prepared by combining equal volumes of FBP diluted 1:1000 with 50 mM sodium tetraborate, 150 mM NaCl, 1.0 mM MgCl2, 0.1 mM ZnCl2, 0.1% rabbit IgG (Sigma, used as a stabilizer), pH 9.3, and anti-FBP-AP conjugate diluted 1:200 with the same buffer.

Landscapes

• Health & Medical Sciences (AREA)
• Immunology (AREA)
• Life Sciences & Earth Sciences (AREA)
• Engineering & Computer Science (AREA)
• Chemical & Material Sciences (AREA)
• Hematology (AREA)
• Urology & Nephrology (AREA)
• Biomedical Technology (AREA)
• Molecular Biology (AREA)
• Microbiology (AREA)
• Analytical Chemistry (AREA)
• Biotechnology (AREA)
• Pathology (AREA)
• Food Science & Technology (AREA)
• Medicinal Chemistry (AREA)
• Physics & Mathematics (AREA)
• Cell Biology (AREA)
• Biochemistry (AREA)
• General Health & Medical Sciences (AREA)
• General Physics & Mathematics (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Peptides Or Proteins (AREA)
• Preparation Of Compounds By Using Micro-Organisms (AREA)

Applications Claiming Priority (2)

Publications (2)

Family

ID=22530614

Family Applications (1)

Country Status (4)

Families Citing this family (6)

Family Cites Families (5)

• 1989
  • 1989-01-27 WO PCT/US1989/000342 patent/WO1989007271A1/en not_active Ceased
  • 1989-01-27 GB GB9015666A patent/GB2233091B/en not_active Expired - Lifetime
  • 1989-01-27 JP JP1502491A patent/JPH03503566A/ja active Pending
  • 1989-01-27 EP EP19890902678 patent/EP0404783A4/en not_active Withdrawn

Also Published As

Similar Documents

Legal Events