EP0465528A1 - Verfahren zum nachweis von nukleinsäure durch binäre amplifikation - Google Patents

Verfahren zum nachweis von nukleinsäure durch binäre amplifikation

Info

Publication number: EP0465528A1
Authority: EP; European Patent Office
Prior art keywords: nucleic acid; sequence; primers; primer; sets
Prior art date: 1989-03-27
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP90905265A

Other languages

English (en)

French (fr)

Inventor

Sydney Brenner

Jeffrey Allan Miller

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

EIDP Inc

Original Assignee

EI Du Pont de Nemours and Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-03-27

Filing date

1990-03-26

Publication date

1992-01-15

1990-03-26 Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co

1992-01-15 Publication of EP0465528A1 publication Critical patent/EP0465528A1/de

Status Withdrawn legal-status Critical Current

Links

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108020004707 nucleic acids Proteins 0.000 title claims abstract description 39
102000039446 nucleic acids Human genes 0.000 title claims abstract description 39
238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 24
230000003321 amplification Effects 0.000 title claims abstract description 23
238000001514 detection method Methods 0.000 title claims abstract description 18
238000000034 method Methods 0.000 title description 33
230000008569 process Effects 0.000 title description 11
238000006243 chemical reaction Methods 0.000 claims abstract description 11
239000013615 primer Substances 0.000 claims description 41
108091028043 Nucleic acid sequence Proteins 0.000 claims description 10
230000000295 complement effect Effects 0.000 claims description 10
230000015572 biosynthetic process Effects 0.000 claims description 6
238000005259 measurement Methods 0.000 claims description 5
238000003786 synthesis reaction Methods 0.000 claims description 5
239000003155 DNA primer Substances 0.000 claims description 4
102000018120 Recombinases Human genes 0.000 claims description 3
108010091086 Recombinases Proteins 0.000 claims description 3
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239000002253 acid Substances 0.000 description 1
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SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
238000004925 denaturation Methods 0.000 description 1
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238000001962 electrophoresis Methods 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
229960005542 ethidium bromide Drugs 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
230000001678 irradiating effect Effects 0.000 description 1
239000006249 magnetic particle Substances 0.000 description 1
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239000012528 membrane Substances 0.000 description 1
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238000010369 molecular cloning Methods 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
238000007899 nucleic acid hybridization Methods 0.000 description 1
239000002853 nucleic acid probe Substances 0.000 description 1
125000003729 nucleotide group Chemical group 0.000 description 1
230000003169 placental effect Effects 0.000 description 1
108091033319 polynucleotide Proteins 0.000 description 1
102000040430 polynucleotide Human genes 0.000 description 1
239000002157 polynucleotide Substances 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
238000013207 serial dilution Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]

Definitions

the second is a 160-base region also located within the GAG pl7 region of HIV I, incorporated into a plasmid (the plasmic incorporating most of the HIV I genome is designated pBH10-R3) , and can be amplified using Primers

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Engineering & Computer Science (AREA)
Immunology (AREA)
Virology (AREA)
Molecular Biology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
Microbiology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Chemical Kinetics & Catalysis (AREA)
AIDS & HIV (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)

EP90905265A 1989-03-27 1990-03-26 Verfahren zum nachweis von nukleinsäure durch binäre amplifikation Withdrawn EP0465528A1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US32899989A	1989-03-27	1989-03-27
US328999		1989-03-27

Publications (1)

Publication Number	Publication Date
EP0465528A1 true EP0465528A1 (de)	1992-01-15

Family

ID=23283411

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP90905265A Withdrawn EP0465528A1 (de)	1989-03-27	1990-03-26	Verfahren zum nachweis von nukleinsäure durch binäre amplifikation

Country Status (5)

Country	Link
EP (1)	EP0465528A1 (de)
JP (1)	JPH04504201A (de)
AU (1)	AU5345790A (de)
CA (1)	CA2012983A1 (de)
WO (1)	WO1990011375A1 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1994003624A1 (en)	1992-08-04	1994-02-17	Auerbach Jeffrey I	Methods for the isothermal amplification of nucleic acid molecules
US5733733A (en) *	1992-08-04	1998-03-31	Replicon, Inc.	Methods for the isothermal amplification of nucleic acid molecules
US5834202A (en)	1992-08-04	1998-11-10	Replicon, Inc.	Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en)	1992-08-04	2001-07-17	Replicon, Inc.	Amplification of nucleic acid molecules via circular replicons
US5614389A (en) *	1992-08-04	1997-03-25	Replicon, Inc.	Methods for the isothermal amplification of nucleic acid molecules
SG63589A1 (en) *	1993-05-14	1999-03-30	Johnson & Johnson Clin Diag	Diagnostic compositions elements methods and test kits for amplification and detection of two or more dna's using primers having matched melting temperatures
EP0937098B1 (de) *	1995-06-07	2002-08-14	Invitrogen Corporation	Rekombinatorische klonierung in vitro unter verwendung genmanipulierter rekombinationsorte
US6143557A (en)	1995-06-07	2000-11-07	Life Technologies, Inc.	Recombination cloning using engineered recombination sites
US6964861B1 (en)	1998-11-13	2005-11-15	Invitrogen Corporation	Enhanced in vitro recombinational cloning of using ribosomal proteins
US6720140B1 (en)	1995-06-07	2004-04-13	Invitrogen Corporation	Recombinational cloning using engineered recombination sites
US5851808A (en)	1997-02-28	1998-12-22	Baylor College Of Medicine	Rapid subcloning using site-specific recombination
DE69836092T2 (de)	1997-10-24	2007-05-10	Invitrogen Corp., Carlsbad	Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
US7351578B2 (en)	1999-12-10	2008-04-01	Invitrogen Corp.	Use of multiple recombination sites with unique specificity in recombinational cloning
DE60042969D1 (de)	1999-03-02	2009-10-29	Life Technologies Corp	Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
ATE391789T1 (de)	1999-12-10	2008-04-15	Invitrogen Corp	Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen
US7244560B2 (en)	2000-05-21	2007-07-17	Invitrogen Corporation	Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7198924B2 (en)	2000-12-11	2007-04-03	Invitrogen Corporation	Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
JP2005504308A (ja) *	2001-10-03	2005-02-10	イセアオテクノロジーズリミテッド	標的分子および分子間相互作用の検出法
DE602004027538D1 (de)	2003-12-01	2010-07-15	Life Technologies Corp	Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4683202A (en) *	1985-03-28	1987-07-28	Cetus Corporation	Process for amplifying nucleic acid sequences
GB8612087D0 (en) *	1986-05-19	1986-06-25	Ici Plc	Hybridisation probes

1990
- 1990-03-23 CA CA002012983A patent/CA2012983A1/en not_active Abandoned
- 1990-03-26 WO PCT/US1990/001535 patent/WO1990011375A1/en not_active Ceased
- 1990-03-26 JP JP2505279A patent/JPH04504201A/ja active Pending
- 1990-03-26 AU AU53457/90A patent/AU5345790A/en not_active Abandoned
- 1990-03-26 EP EP90905265A patent/EP0465528A1/de not_active Withdrawn

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9011375A1 *

Also Published As

Publication number	Publication date
CA2012983A1 (en)	1990-09-27
JPH04504201A (ja)	1992-07-30
WO1990011375A1 (en)	1990-10-04
AU5345790A (en)	1990-10-22

Legal Events

Date	Code	Title	Description
1991-11-29	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
1992-01-15	17P	Request for examination filed	Effective date: 19910919
1992-01-15	AK	Designated contracting states	Kind code of ref document: A1 Designated state(s): AT BE DE DK FR GB IT LU NL SE
1992-01-16	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE APPLICATION HAS BEEN WITHDRAWN
1992-03-04	18W	Application withdrawn	Withdrawal date: 19920115

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US5538872A (en)	1996-07-23	Method of preparing nucleotide probes using a bridging complement
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