EP0517761B1 - Lipase enthaltendes flüssiges einweichmittel und dessen verwendung - Google Patents

Lipase enthaltendes flüssiges einweichmittel und dessen verwendung Download PDF

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Publication number
EP0517761B1
EP0517761B1 EP19910905035 EP91905035A EP0517761B1 EP 0517761 B1 EP0517761 B1 EP 0517761B1 EP 19910905035 EP19910905035 EP 19910905035 EP 91905035 A EP91905035 A EP 91905035A EP 0517761 B1 EP0517761 B1 EP 0517761B1
Authority
EP
European Patent Office
Prior art keywords
spotter
lipase
fat
alcohol
laundry
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP19910905035
Other languages
English (en)
French (fr)
Other versions
EP0517761A1 (de
Inventor
Erik Gormsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK53090A external-priority patent/DK53090D0/da
Priority claimed from DK77290A external-priority patent/DK77290D0/da
Priority claimed from DK11891A external-priority patent/DK11891D0/da
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Priority to AT91905035T priority Critical patent/ATE101642T1/de
Publication of EP0517761A1 publication Critical patent/EP0517761A1/de
Application granted granted Critical
Publication of EP0517761B1 publication Critical patent/EP0517761B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase

Definitions

  • the invention relates to a lipase containing liquid pre-spotter and to a use of such pre-spotter.
  • the purpose of the invention is the provision of a simple lipase containing liquid pre-spotter, which exhibits an improved fat and oil removing effect and an improved lipase stability.
  • the lipase containing and surfactant free liquid pre-spotter according to the invention comprises a liquid mixture of an alcohol and water, with an alcohol content above 20% by weight, and an effective amount of lipase, whereby the balance is water. It should be noted that the pre-spotter according to the invention does not contain any surfactant, and thus exhibits a simple composition, in spite of the fact that the pre-spotter according to the invention is very effective, as demonstrated in the following examples.
  • alcohol comprises both monovalent alcohols like ethanol and polyvalent alcohols (polyols) like propylene glycol. If the alcohol content is below 10% by weight, the effect and the stability of the lipase tend to be unsatisfactory.
  • an effective amount of lipase means an amount, which is able to remove fat and oil stains effectively, when evaluated by swatch tests described later in this specification. It is intended that all lipases can be used in the pre-spotter according to the invention. Reference can be made to Research Disclosure June 1988, No. 290, 29056.
  • DE-A-3 003 766 describes a lipase containing liquid pre-spotter which besides an alkanolamine contains at least 10% of a surfactant.
  • This is a typical example of an aqueous, enzyme containing pre-spotter, as this kind of prior art pre-spotter all contain a surfactant.
  • the pre-spotter according to the invention is surfactant free and does not need to contain alkalolamine.
  • the effect of the pre-spotter according to the invention is as good as or better than the known surfactant containing pre-spotter and the pre-spotter according to the invention is cheaper than the prior art pre-spotters, because the pre-spotter according to the invention does not contain the relatively expensive surfactant.
  • the pre-spotter further comprises a lipase stabilizer.
  • a lipase stabilizer examples include CaCl2 (e.g. 0.1-0.5%) or a protease inhibitor (such as borate and formate).
  • the alcohol is a mono- or polyvalent alcohol with 2-3 carbon atoms, preferably ethanol, propylene glycol, glycerol or a mixture of two or more of these. It has been found that the fat and oil removing effect is most satisfactory with these alcohols.
  • the lipase activity is at least 10,000 LU/liter of liquid pre-spotter. It has been found that the time interval between application of pre-spotter and main wash is undue long in order to obtain an efficient fat and oil removal, if the lipase activity is less than 10,000 LU/liter of liquid pre-spotter. On the other hand, for economic reasons a suitable upper limit for the lipase activity is 5,000,000 LU/liter of liquid pre-spotter.
  • the lipase is Lipolase®. It has been found that the fat and oil removal is very efficient in this embodiment.
  • the pre-spotter contains an antimicrobial agent.
  • antimicrobial agents are inorganic salts (such as NaCl), sugars (such as sucrose and glucose), organic acids (such as benzoic, sorbic, propionic, lactic and formic acids) which are generally effective in amounts of 0.01-2% at low pH (below 5).
  • stabilizing agents are antioxidants (such as sulphur dioxide), 1,2-benz-iso-thioazolin-3-one (BIT) and parabens. Some of these may also serve to improve enzyme stability.
  • the pH value is between 4 and 10, preferably between 5 and 9.
  • the stability optimum and the activity optimum of the lipase should be taken into consideration.
  • the invention comprises a use of the pre-spotter according to the invention, and this use is characterized by the fact that the pre-spotter is applied locally to the fat or oil spots on the laundry, that the laundry is left alone for a certain time period, and the the laundry subsequently is exposed to a low temperature washing process.
  • the certain time period alluded to above is normally at least 5 minutes, but it can be shorter in case the lipase activity in the pre-spotter is extraordinarily high.
  • the lipase activity is measured in LU lipase activity units.
  • the LU activity determination method is based on hydrolysis of tributyrin in a pH-stat.
  • 1 LU lipase Unit
  • 1 LU lipase Unit is the amount of enzyme which liberates 1 ⁇ mole titratable butyric acid per minute at 30°C and pH 7.0 with gum arabic as an emulsifier. Further details are given in Novo Nordisk analytical Method AF 95/5, available on request from Novo Nordisk A/S, Novo Allé, DK-2880 Bagsvaerd, Denmark.
  • Embodiments of liquid pre-spotter is applied to the fat stain on a test swatch by means of a pipette.
  • the amount of pre-spotter used depends on the size of the stain and on the kind of fabric; typically 0.5-3 ml of pre-spotter is satisfactory.
  • After 5-30 minutes of incubation the swatches are washed in a Laboratory washing machine (Launder-o-meter or Terg-o-tometer) at low temperature (20-40°C).
  • the detergent used in the washing process is a standard powder or liquid detergent.
  • the swatches are made from coloured (e.g. blue or green) cotton fabric.
  • the fabric is cut into test pieces with the dimensions 12x12 cm, and 75-150 ⁇ l of fatty material (e.g. lard, margarine, butter, or olive oil) is applied to the center of each swatch.
  • fatty material e.g. lard, margarine, butter, or olive oil
  • the amount of fatty material applied depends on the type of fatty material used.
  • the fatty material is heated to approx. 70°C before application in order to facilitate the penetration of fatty material into the fabric.
  • the stained fabric was aged at room temperature for 1 day before treatment.
  • the swatches After washing and drying the swatches are gently ironed. The swatches are visually inspected and compared to reference swatches which have been identically treated but without any lipase added to the pre-spotter formulation. In order to quantify the efficiency of the lipase, extraction of the residual fatty material can be carried out (Soxhlet, chloroform, 5 hours).
  • Stain Lard on green cotton sheet. 75 ⁇ l of lard was applied on each swatch. After stain application, the swatches were heat treated in an oven for 30 minutes at 75°C in order to obtain a better distribution of the stain.
  • Pre-spotting 1 ml of pre-spotter is applied with a pipette on the centre of the stains and subsequently the swatches are incubated for 15 minutes at room temperature.
  • the pre-spotter formulations contained 1% Lipolase® 100L (100,000 LU/g) and 99% (w/w) of a mixture of water and propylene glycol. Accordingly the lipase activity in the pre-spotters was 1000 LU/g.
  • Example 2 The same kind of test material and the same pre-spotting, washing, and evaluation method as described in Example 1 was used; the only difference being that the dosage of Lipolase® 100 L was varied between 0 and 5%.
  • Example 2 The same experiments as described in Example 2 were carried out, the only difference being that four lipases of different origin (2 fungal, 1 bacterial and 1 from yeast) were investigated. The lipases were compared on an LU-activity basis.
  • test material The same kind of test material and the same pre-spotting washing, and evaluation method as described in Example 1 was used.
  • the lipase activity in the pre-spotter was 5000 LU/g.
  • Washing Immediately after pre-spotting the swatches were washed in a Terg-o-tometer (30°C, 30 minutes, 18° dH, pH 9.5) with a commercial European powder detergent (5 g/l). Evaluation: The performance of the pre-spotter was evaluated by the same test panel method as described in Example 1. Results: The mean scores (12 persons) were as follows:
  • Lipolase® The enzymatic stability of Lipolase® was investigated in 5 different pre-spotter formulations.
  • the pre-spotter formulations were stored at 30°C in closed bottles and the activity was followed over a 2 months period.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Detergent Compositions (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)

Claims (8)

  1. Lipase enthaltendes und tensidfreies flüssiges Einweichmittel, das eine flüssige Mischung aus einem Alkohol und Wasser, mit einem Alkoholgehalt über 20 Gew.-%, und eine wirksame Menge an Lipase umfaßt, wobei der Rest Wasser ist.
  2. Einweichmittel nach Anspruch 1, das außerdem einen Lipasestabilisator umfaßt.
  3. Einweichmittel nach den Ansprüchen 1 bis 2, in dem der Alkohol Ethanol, Propylenglykol, Glycerin oder eine Mischung aus zwei oder mehreren derselben ist.
  4. Einweichmittel nach den Ansprüchen 1 bis 3, in dem die Lipaseaktivität wenigstens 10.000 LU/Liter flüssiges Einweichmittel beträgt.
  5. Einweichmittel nach den Ansprüchen 1 bis 4, wobei die Lipase Lipolase® ist.
  6. Einweichmittel nach den Ansprüchen 1 bis 5, wobei das Einweichmittel ein antimikrobielles Mittel enthält.
  7. Einweichmittel nach den Ansprüchen 1 bis 6, wobei der pH-Wert zwischen 4 und 10, vorzugsweise zwischen 5 und 9 liegt.
  8. Verwendung des Einweichmittels nach den Ansprüchen 1-7, gekennzeichnet durch die Tatsache, daß das Einweichmittel örtlich auf die Fett- oder Ölflecke auf der Wäsche aufgebracht wird, daß die Wäsche für einen bestimmten Zeitraum sich überlassen bleibt und daß die Wäsche anschließend einem Niedertemperatur-Waschverfahren ausgesetzt wird.
EP19910905035 1990-03-01 1991-02-28 Lipase enthaltendes flüssiges einweichmittel und dessen verwendung Expired - Lifetime EP0517761B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT91905035T ATE101642T1 (de) 1990-03-01 1991-02-28 Lipase enthaltendes fluessiges einweichmittel und dessen verwendung.

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DK53090A DK53090D0 (de) 1990-03-01 1990-03-01
DK530/90 1990-03-01
DK77290A DK77290D0 (da) 1990-03-01 1990-03-26 Lipaseholdig flydende pre-spotter og anvendelse af en saadan pre-spotter
DK772/90 1990-03-26
DK118/91 1991-01-24
DK11891A DK11891D0 (da) 1991-01-24 1991-01-24 Lipaseholdig flydende pre-spotter og anvendelse af en saadan pre-spotter.

Publications (2)

Publication Number Publication Date
EP0517761A1 EP0517761A1 (de) 1992-12-16
EP0517761B1 true EP0517761B1 (de) 1994-02-16

Family

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Family Applications (1)

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EP19910905035 Expired - Lifetime EP0517761B1 (de) 1990-03-01 1991-02-28 Lipase enthaltendes flüssiges einweichmittel und dessen verwendung

Country Status (6)

Country Link
EP (1) EP0517761B1 (de)
JP (1) JP2930719B2 (de)
DE (1) DE69101219T2 (de)
DK (1) DK0517761T3 (de)
ES (1) ES2051122T3 (de)
WO (2) WO1991013142A1 (de)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014200658A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from promicromonospora vindobonensis
WO2014200657A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces xiamenensis
WO2014200656A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces umbrinus
WO2014204596A1 (en) 2013-06-17 2014-12-24 Danisco Us Inc. Alpha-amylase from bacillaceae family member
WO2015050724A1 (en) 2013-10-03 2015-04-09 Danisco Us Inc. Alpha-amylases from a subset of exiguobacterium, and methods of use, thereof
WO2015050723A1 (en) 2013-10-03 2015-04-09 Danisco Us Inc. Alpha-amylases from exiguobacterium, and methods of use, thereof
WO2015077126A1 (en) 2013-11-20 2015-05-28 Danisco Us Inc. Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof
WO2017173190A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
WO2017173324A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2152246T3 (es) * 1991-12-20 2001-02-01 Novo Nordisk As Eliminacion de esteres hidrofobicos de los tejidos.
US5605881A (en) * 1993-09-03 1997-02-25 Minolta Co., Ltd. Cleaning liquid for recycling copy medium for electrophotography
CA2161975C (en) * 1994-03-21 2000-02-01 Jeanne A. O'brien Stable enzyme-containing aqueous laundry prespotting composition
US5510052A (en) * 1994-08-25 1996-04-23 Colgate-Palmolive Co. Enzymatic aqueous pretreatment composition for dishware
CN109844085A (zh) * 2016-09-13 2019-06-04 诺维信公司 洗涤剂组合物、洗涤剂组合物的用途和洗涤纺织品的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8000452A (nl) * 1979-02-07 1980-08-11 Unilever Nv Voorbehandelingsmiddel voor wasgoed.
DE3223568A1 (de) * 1982-06-24 1983-12-29 Peter 5275 Bergneustadt Penz jun. Detachiermittel und detachierverfahren
NL8900267A (nl) * 1989-02-03 1990-09-03 Douwe Egberts Tabaksfab Vloeibaar reinigingsmiddel.

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014200658A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from promicromonospora vindobonensis
WO2014200657A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces xiamenensis
WO2014200656A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces umbrinus
WO2014204596A1 (en) 2013-06-17 2014-12-24 Danisco Us Inc. Alpha-amylase from bacillaceae family member
WO2015050724A1 (en) 2013-10-03 2015-04-09 Danisco Us Inc. Alpha-amylases from a subset of exiguobacterium, and methods of use, thereof
WO2015050723A1 (en) 2013-10-03 2015-04-09 Danisco Us Inc. Alpha-amylases from exiguobacterium, and methods of use, thereof
WO2015077126A1 (en) 2013-11-20 2015-05-28 Danisco Us Inc. Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof
WO2017173190A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
WO2017173324A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods

Also Published As

Publication number Publication date
DE69101219D1 (de) 1994-03-24
WO1991013141A1 (en) 1991-09-05
WO1991013142A1 (en) 1991-09-05
ES2051122T3 (es) 1994-06-01
DE69101219T2 (de) 1994-06-01
JP2930719B2 (ja) 1999-08-03
JPH05504368A (ja) 1993-07-08
EP0517761A1 (de) 1992-12-16
DK0517761T3 (da) 1994-05-09

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