EP0673203A1 - Procede pour produire un concentre proteique de lactoserum non denature - Google Patents

Procede pour produire un concentre proteique de lactoserum non denature

Info

Publication number
EP0673203A1
EP0673203A1 EP94900688A EP94900688A EP0673203A1 EP 0673203 A1 EP0673203 A1 EP 0673203A1 EP 94900688 A EP94900688 A EP 94900688A EP 94900688 A EP94900688 A EP 94900688A EP 0673203 A1 EP0673203 A1 EP 0673203A1
Authority
EP
European Patent Office
Prior art keywords
microfiltration
whey protein
milk
serum albumin
protein concentrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP94900688A
Other languages
German (de)
English (en)
Inventor
Gustavo Apartment 1003 Bounous
Sylvie Turgeon
Bernard Aurouze
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Immunotec Research Corp
Original Assignee
Immunotec Research Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunotec Research Corp filed Critical Immunotec Research Corp
Publication of EP0673203A1 publication Critical patent/EP0673203A1/fr
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/20—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey
    • A23J1/205—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey from whey, e.g. lactalbumine
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/14—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment
    • A23C9/142—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment by dialysis, reverse osmosis or ultrafiltration
    • A23C9/1422—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment by dialysis, reverse osmosis or ultrafiltration by ultrafiltration, microfiltration or diafiltration of milk, e.g. for separating protein and lactose; Treatment of the UF permeate
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C2210/00—Physical treatment of dairy products
    • A23C2210/20—Treatment using membranes, including sterile filtration
    • A23C2210/206—Membrane filtration of a permeate obtained by ultrafiltration, nanofiltration or microfiltration
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C2210/00—Physical treatment of dairy products
    • A23C2210/20—Treatment using membranes, including sterile filtration
    • A23C2210/208—Removal of bacteria by membrane filtration; Sterile filtration of milk products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • thermosensitive proteins serum albumin and immunoglobulin were partially heat denatured and hence precipitated in the curd.
  • Said related applications describe experiments where W.P.C. was prepared using the lowest level of heat treatment of milk compatible with safety standards, so as to obtain a whey protein distribution having a high content of the thermolabile serum albumin and immunoglobin.
  • Dietary W.P.C. produced with low level pasteurization improves systemic humoral immune response, increases the resistance of target cells against the carcinogenic effect of chemical carcinogens such as dimethylhydrazine, improves resistance to pneumococcal infection, and provides a sustained increase of tissue glutathione.
  • thermosensitivity of the immunoglobulins is very similar to that of BSA (2.4). Although the amino acid sequence of most immunoglobulins in cow's milk is not known, we know that these large molecules are very rich in Cysteine hence they may contain Glu-Cys groups. Therefore they might exert upon digestion and absorption an activity similar to that of BSA.
  • the major drawback with low temperature milk pasteurization are: a) Some degree of heat denaturation, hence precipitation of the thermosensitive proteins; b) The possibility of insufficient bacterial reduction; c) The difficulty in maintaining the delicate balance between adequate pasteurization and the avoidance of protein denaturation. This is likely to cause problems of control on a factory scale; d) The cost of low temperature pasteurization.
  • the object of this invention is to provide an improved process for preparing a whey protein concentrate that has adequate bacterial reduction without protein denaturation. It is a particular object to provide a process that will achieve a whey protein concentrate having a serum albumin of at least 10% and adequate bacterial reduction. This approximately 10% level of serum albumin was found in our studies to be important for the achievement of sustained increase of tissue glutathione for the properties such as improved systemic humoral immune response described above (References 2 and 3) .
  • Microfiltration using ceramic membranes is a membrane based procedure which allows the separation of particles ranging in size between 0.1 and 10 microns.
  • the casein in raw milk occurs in the form of a colloidal dispersion.
  • the particles of this dispersion range from 20 to 200 n in diameter and are generally referred to as casein micelles.
  • a recent study (7) has shown that a microfiltration membrane containing pores with average diameter of 0.2 micron will selectively retain from skim milk the casein micelles.
  • This study concentrated on the separation of casein.
  • the milk that was used was skim milk that had been pasteurized at a temperature of 72°C. which would have resulted in denaturation.
  • the study does not indicate the serum albumin content of the permeate, it is certain that it would be a low value.
  • a process for producing an undenatured serum albumin content of at least about 10% comprising the following steps, each of which is conducted at a temperature not greater than about 50°C ( ⁇ 50°):
  • Figure 1 is a schematic representation of the process of this invention for producing a whey protein concentrate with immunoenhancing properties.
  • Protein content (N x 6.38) and total lipids of samples were determined in duplicate respectively by the standard method of Kjeldahl and the method of Mojonnier.
  • Moisture content was determined in duplicate by the AOCS method (9) .
  • Total coliforms count was determined following incubation at 37°C for 18 h in brilliant green using the most probable number method.
  • Total bacteria count (aerobic esophiles) was determined following incubation at 32°C for 48 h in PCA medium. Both methods are approved by the International Dairy Federation and American Public Health Association.
  • Table 1 shows the operating conditions during microfiltration of skim raw bovine milk using a ceramic membrane, as previously described having a pore size of 1.4 microns. TABLE 1 Operating conditions during microfiltration for bacterial count reduction (1.4 ).
  • Raw milk should be used that has been skimmed at a temperature not in excess of about 50°C.
  • the best temperature for skimming to a level of 0.05% of fat is 45°C to 50°C.
  • the temperature used during microfiltration of the raw milk should be in the range about 40°C to 50°C. As previously noted denaturation of serum albumin does not begin to occur until about 55°C.
  • Table 2 shows the microbial counts of the milk following microfiltration. We have noted a 2-3 log reduction in bacterial counts in accordance with the literature (5) . This confirms the efficacy of the method. As expected, the lipids are in the retentate. The protein content of the permeate is only marginally different from that of the retentate and there is no difference between permeate and retentate in the type of proteins as measured by electrophoresis. Hence no selective retention of proteins is noted over a 1.4 ⁇ membrane.
  • microfiltration is followed by diafiltration which involves adding distilled water to the retentate. 1 volume of retentate to 1 volume of water is used and this procedure is performed two or three times. TABLE 2 .
  • the microbial counts of the permeate compare favourably with standards applicable to conventional pasteurization.
  • the second stage involved the separation of the casein micelles with a 0.1 ⁇ membrane.
  • the parameters are illustrated in Table 3. It should be noted that these values may vary throughout the procedure depending on conditions such as clogging of membrane pores, increased viscosity of the retentate etc.
  • the results of the process are given in Table 4.
  • Bovine serum albumin passes ' through the membrane.
  • the protein composition of the final product in powder form after concentration by ultrafiltration and lyophylization (Table 4) meets the requirements previously identified by us as essential for the development of immunoenhancing activity and tissue GSH promotion: serum albumin concentration around 10% and minimal degree of denaturation.
  • Table 5 and Table 6 are presented for comparison the concentrations of serum albumin in current commercially available W.P.C. , s and the nitrogen solubility index determined by De Wit in some W.P.C. products.
  • the retentate from the second stage was subjected to ultrafiltration on a pilot scale using a Romicon cartridge (1.5 ⁇ qm) with a cut off of 50,000 Dalton to provide a powder.
  • the temperature during ultrafiltration was ⁇ 50°C. Table 4 shows the results obtained by the process.
  • PRODUCT BOVINE SERUM ALBUMIN in % of Total Whey Protein
  • the process of this invention therefore provides a practical procedure for making undenatured whey protein concentrate. Furthermore it has the advantage of permitting separate recovery of the casein. This is a useful by-product that contributes to the practical economies of the process.

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Water Supply & Treatment (AREA)
  • Biochemistry (AREA)
  • Dairy Products (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Un procédé pour produire un concentré protéique de lactosérum non dénaturé consiste à effectuer une microfiltration du lait cru écrémé pour assurer une réduction bactérienne, suivie d'une microfiltration pour séparer la caséine et d'une ultrafiltration pour donner un concentré protéique de lactosérum présentant une teneur en sérine d'environ 10 % ou plus.
EP94900688A 1992-12-11 1993-12-09 Procede pour produire un concentre proteique de lactoserum non denature Ceased EP0673203A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US98918692A 1992-12-11 1992-12-11
US989186 1992-12-11
PCT/CA1993/000518 WO1994013148A1 (fr) 1992-12-11 1993-12-09 Procede pour produire un concentre proteique de lactoserum non denature

Publications (1)

Publication Number Publication Date
EP0673203A1 true EP0673203A1 (fr) 1995-09-27

Family

ID=25534846

Family Applications (1)

Application Number Title Priority Date Filing Date
EP94900688A Ceased EP0673203A1 (fr) 1992-12-11 1993-12-09 Procede pour produire un concentre proteique de lactoserum non denature

Country Status (4)

Country Link
EP (1) EP0673203A1 (fr)
AU (1) AU5557994A (fr)
CA (1) CA2151506A1 (fr)
WO (1) WO1994013148A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9775366B2 (en) 2012-04-10 2017-10-03 Kraft Foods R & D, Inc. Process for producing cream cheese

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU700748B2 (en) * 1994-09-16 1999-01-14 New Zealand Dairy Board Physical separation of casein and whey proteins
US5707678A (en) * 1995-04-12 1998-01-13 Galagen Inc. Method for microfiltration of milk or colostral whey
CA2165937A1 (fr) * 1995-05-09 1996-11-10 Immunotec Research Corporation Ltd. Methode de preparation d'un concentre proteinique non caseique
CA2197515A1 (fr) * 1996-07-15 1998-01-16 Reyad Mahmoud Methodes pour traiter les produits laitiers
CA2264630C (fr) 1996-09-06 2006-12-12 Pall Corporation Procede et systeme de separation par cisaillement
ES2226466T3 (es) * 1998-11-24 2005-03-16 Societe Des Produits Nestle S.A. Procedimiento de preparacion de una composicion proteinica y de una formula infantil que la contiene.
DK174656B1 (da) 1999-06-04 2003-08-11 Apv Pasilac As Fremgangsmåde og anlæg til behandling af mælk
NL1015851C2 (nl) * 2000-08-01 2002-02-05 Friesland Brands Bv Werkwijze voor het filtreren van melk.
LT5362B (lt) * 2005-11-02 2006-10-25 Vytautas Fedaravicius KAZEINO GAMYBOS BuDAS IR IRENGINYS
NL1033698C2 (nl) 2007-04-16 2008-10-20 Friesland Brands Bv Functioneel serumeiwitproduct voor toepassing in kindervoeding en therapeutische samenstellingen, en werkwijzen ter bereiding daarvan.
JP5762951B2 (ja) 2008-05-15 2015-08-12 ダブリュ．・ヘルス・エル．ピー．Ｗ． Ｈｅａｌｔｈ Ｌ．Ｐ． 分泌性免疫グロブリンの豊富な乳画分の製造方法
US9055752B2 (en) 2008-11-06 2015-06-16 Intercontinental Great Brands Llc Shelf-stable concentrated dairy liquids and methods of forming thereof
GB2469500B (en) 2009-04-16 2012-06-06 Cambridge Display Tech Ltd Method of forming a polymer
WO2011051557A1 (fr) 2009-10-28 2011-05-05 Valio Ltd Produit à base de protéines lactosériques et son procédé de préparation
UA112972C2 (uk) 2010-09-08 2016-11-25 Інтерконтінентал Грейт Брендс ЛЛС Рідкий молочний концентрат з високим вмістом сухих речовин
FI124323B (fi) 2011-02-18 2014-06-30 Valio Oy Maitopohjainen tuote ja menetelmä sen valmistamiseksi
NL2006662C2 (en) * 2011-04-26 2012-10-29 Friesland Brands Bv Method of making a milk protein composition.
JP2014520549A (ja) * 2011-07-13 2014-08-25 フリースランド・ブランズ・ビー・ブイ タンパク質の消化性が改善された組成物
HK1200062A1 (en) * 2011-07-13 2015-07-31 弗里斯兰品牌有限公司 Dairy based compositions with low lps
EP2548457A1 (fr) * 2011-07-18 2013-01-23 Ludwig-Maximilians-Universität München Préparation de lait cru à utiliser pour empêcher ou traiter l'asthme et autres maladies allergiques chez les nourrissons et les enfants
WO2013011040A1 (fr) * 2011-07-18 2013-01-24 Ludwig-Maximilians-Universität München Préparation à base de lait cru pouvant être utilisée en vue de la prévention ou du traitement de l'asthme et d'autres affections allergiques chez le bébé et l'enfant
BR112014022527B1 (pt) * 2012-03-12 2020-05-05 Nutricia Nv processo para a preparação de um produto base de fórmula infantil, composição, e produto base de fórmula infantil obtenível pelo processo
CN102630803A (zh) * 2012-05-15 2012-08-15 东北农业大学 母乳化牛乳蛋白及其制备方法
CN116869047A (zh) * 2013-01-23 2023-10-13 阿拉食品公司 生产β-酪蛋白组合物和相关产品的方法
FI127870B (en) 2015-08-31 2019-04-15 Valio Oy Microparticulated ideal whey protein product, process for its preparation and its use
US11918006B2 (en) 2016-05-12 2024-03-05 Arla Foods Amba Whey preparation for improving brain development
EP3298903B1 (fr) 2016-09-27 2020-11-18 DMK Deutsches Milchkontor GmbH Lait en poudre pauvre en germes avec haut index de protéines de lactosérum (iv)
CN110663773A (zh) * 2019-01-08 2020-01-10 东北农业大学 一种从鲜奶中分离天然乳清蛋白的方法及应用
WO2021013862A1 (fr) 2019-07-23 2021-01-28 Frieslandcampina Nederland B.V. Composition nutritionnelle comprenant de la matière grasse de lait et de l'immunoglobuline
CN112931677B (zh) * 2021-03-09 2024-02-06 江南大学 一种高活性乳清蛋白及制备方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH576235A5 (en) * 1973-08-20 1976-06-15 Mueller Hans Maennedorf Production of sterile milk protein - by multi-stage filtration
US4112123A (en) * 1976-07-21 1978-09-05 Beatrice Foods Co. Nutritionally balanced single food composition and method of production
FR2631785A1 (fr) * 1988-05-27 1989-12-01 Agronomique Inst Nat Rech Procede de fractionnement des proteines du lait humain, conduisant a la production, notamment de lactoferrine et d'(alpha)-lactalbumine, et produits obtenus
FR2681218B1 (fr) * 1991-09-16 1995-08-11 Normandie Laitiere Procede de traitement des laits permettant au moins de conserver leur aptitude fromagere.
US5169666A (en) * 1991-11-14 1992-12-08 The United States Of America As Represented By The Secretary Of Agriculture Preparation of simulated human milk protein by low temperature microfiltration

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9413148A1 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9775366B2 (en) 2012-04-10 2017-10-03 Kraft Foods R & D, Inc. Process for producing cream cheese

Also Published As

Publication number Publication date
WO1994013148A1 (fr) 1994-06-23
CA2151506A1 (fr) 1994-06-23
AU5557994A (en) 1994-07-04

Similar Documents

Publication Publication Date Title
WO1994013148A1 (fr) Procede pour produire un concentre proteique de lactoserum non denature
FI67654B (fi) Foerfarande foer framstaellning av en rikligt alfa-lakta-albumin innehaollande produkt
Rosenberg Current and future applications for membrane processes in the dairy industry
AU664934B2 (en) Process for the recovery of alpha-lactalbumin and beta-lactoglobulin from a whey protein product
JP4250254B2 (ja) ホエータンパク質濃縮物及びその製造法
EP0008242B1 (fr) Procédé de traitement d'un matériau protéinique et produit alimentaire contenant un matériau protéinique traité par ce procédé
Punidadas et al. Separation of milk proteins into fractions rich in casein or whey proteins by cross flow filtration
US4954361A (en) Hypoallergenic milk products and process of making
WO1996008155A1 (fr) Separation physique de caseine et de proteines de lactoserum
CN112423596B (zh) 乳级分利用正向渗透的生产和分离
Maubois et al. Milk microfiltrate, a convenient starting material for fractionation of whey proteins and derivatives
US20030026845A1 (en) Process for preparing protein isolate from milk, whey, colostrum, and the like
van Hekken et al. Use of cold microfiltration to produce unique ${\beta} $-casein enriched milk gels
US6117470A (en) Method of producing aseptic consumer milk
AU692508B2 (en) Process for making undenatured whey protein concentrate
JP7737376B2 (ja) 最終ナノろ過工程による乳画分の製造及び分離
WO1999055168A1 (fr) Procede de preparation d'un colostrum
Schreiber et al. Rennet coagulation of heated milk concentrates
WO1996035336A1 (fr) Procede permettant de produire un concentre de proteines de lactoserum non denaturees
EP2098122A1 (fr) Fraction de peptone de protéose
US5112636A (en) Hypoallergenic butter and process of making
RU2535876C2 (ru) Способ переработки молока и продукт, полученный этим способом
RU2204262C2 (ru) Способ получения биологически активного белкового концентрата, обогащенного панкреатической рибонуклеазой а, ангиогенином и лизоцимом, из молочного ультрафильтрата
JPH09110896A (ja) 牛乳プロテオースペプトンのコンポーネント３の精製法
Özer et al. Comparison of different methods for beta lactoglobulin isolation

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 19950621

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

17Q First examination report despatched

Effective date: 19951006

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: IMMUNOTEC RESEARCH CORPORATION LTD.

GRAG Despatch of communication of intention to grant

Free format text: ORIGINAL CODE: EPIDOS AGRA

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN REFUSED

18R Application refused

Effective date: 19970130