EP0702081A3 - Matrix für Gewebekultur, Verfahren zur Kultivierung von Gewebe, Verfahren zum Fixieren von kultiviertem Gewebe und fixierte Kunsthaut - Google Patents

Matrix für Gewebekultur, Verfahren zur Kultivierung von Gewebe, Verfahren zum Fixieren von kultiviertem Gewebe und fixierte Kunsthaut Download PDF

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Publication number
EP0702081A3
EP0702081A3 EP19950114741 EP95114741A EP0702081A3 EP 0702081 A3 EP0702081 A3 EP 0702081A3 EP 19950114741 EP19950114741 EP 19950114741 EP 95114741 A EP95114741 A EP 95114741A EP 0702081 A3 EP0702081 A3 EP 0702081A3
Authority
EP
European Patent Office
Prior art keywords
tissue
culturing
matrix
gelatin
artificial skin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP19950114741
Other languages
English (en)
French (fr)
Other versions
EP0702081A2 (de
EP0702081B1 (de
Inventor
Katsuyasu Morota
Shinichiro Morita
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gunze Ltd
Original Assignee
Gunze Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gunze Ltd filed Critical Gunze Ltd
Priority to EP20030015613 priority Critical patent/EP1361265B1/de
Publication of EP0702081A2 publication Critical patent/EP0702081A2/de
Publication of EP0702081A3 publication Critical patent/EP0702081A3/de
Application granted granted Critical
Publication of EP0702081B1 publication Critical patent/EP0702081B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/249921—Web or sheet containing structurally defined element or component
    • Y10T428/249953—Composite having voids in a component [e.g., porous, cellular, etc.]
    • Y10T428/249961—With gradual property change within a component
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/249921—Web or sheet containing structurally defined element or component
    • Y10T428/249953—Composite having voids in a component [e.g., porous, cellular, etc.]
    • Y10T428/249981—Plural void-containing components
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
EP19950114741 1994-09-19 1995-09-19 Matrix für Gewebekultur, Verfahren zur Kultivierung von Gewebe, Verfahren zum Fixieren von kultiviertem Gewebe und fixierte Kunsthaut Expired - Lifetime EP0702081B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP20030015613 EP1361265B1 (de) 1994-09-19 1995-09-19 Verfahren zum Fixieren von kultiviertem Gewebe und fixierte Kunsthaut

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP26304794A JP2858087B2 (ja) 1994-09-19 1994-09-19 組織培養用基材及び組織培養法
JP263047/94 1994-09-19
JP26304794 1994-09-19

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP20030015613 Division EP1361265B1 (de) 1994-09-19 1995-09-19 Verfahren zum Fixieren von kultiviertem Gewebe und fixierte Kunsthaut

Publications (3)

Publication Number Publication Date
EP0702081A2 EP0702081A2 (de) 1996-03-20
EP0702081A3 true EP0702081A3 (de) 2000-01-26
EP0702081B1 EP0702081B1 (de) 2006-03-15

Family

ID=17384129

Family Applications (2)

Application Number Title Priority Date Filing Date
EP20030015613 Expired - Lifetime EP1361265B1 (de) 1994-09-19 1995-09-19 Verfahren zum Fixieren von kultiviertem Gewebe und fixierte Kunsthaut
EP19950114741 Expired - Lifetime EP0702081B1 (de) 1994-09-19 1995-09-19 Matrix für Gewebekultur, Verfahren zur Kultivierung von Gewebe, Verfahren zum Fixieren von kultiviertem Gewebe und fixierte Kunsthaut

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP20030015613 Expired - Lifetime EP1361265B1 (de) 1994-09-19 1995-09-19 Verfahren zum Fixieren von kultiviertem Gewebe und fixierte Kunsthaut

Country Status (4)

Country Link
US (2) US6051425A (de)
EP (2) EP1361265B1 (de)
JP (1) JP2858087B2 (de)
DE (2) DE69534869T2 (de)

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JP2858087B2 (ja) * 1994-09-19 1999-02-17 グンゼ株式会社 組織培養用基材及び組織培養法
JPH10136977A (ja) * 1996-11-11 1998-05-26 Toyobo Co Ltd 組織付属器官様構造体を含む人工組織およびその製造方法
KR100298846B1 (ko) * 1998-09-24 2003-10-22 한국원자력연구소 중화키토산스폰지또는중화키토산/콜라겐혼합스폰지를이용한인공진피
JP2000125855A (ja) * 1998-10-21 2000-05-09 Gunze Ltd 人工皮膚
WO2001011007A2 (en) * 1999-08-10 2001-02-15 Acorda Therapeutics, Inc. Bioartificial device for propagation of tissue, preparation and uses thereof
JP2005506839A (ja) * 2001-10-02 2005-03-10 ベクトン・ディキンソン・アンド・カンパニー 細胞外マトリックスおよび他の分子を使用する幹細胞の増殖および分化
US7923431B2 (en) 2001-12-21 2011-04-12 Ferrosan Medical Devices A/S Haemostatic kit, a method of preparing a haemostatic agent and a method of promoting haemostatis
US20040062882A1 (en) * 2002-09-30 2004-04-01 Andrea Liebmann-Vinson Cell adhesion resisting surfaces
US7955288B2 (en) 2002-12-11 2011-06-07 Ferrosan Medical Devices A/S Gelatine-based materials as swabs
JP4247432B2 (ja) * 2003-05-07 2009-04-02 独立行政法人理化学研究所 凹凸を有するハニカム構造体フィルム
ES2222093A1 (es) * 2003-07-01 2005-01-16 Advanced In Vitro Cell Technologies, S.L. Metodo para el almacenamiento y/o transporte de cultivos celulares in vitro.
US7375077B2 (en) 2003-09-19 2008-05-20 The Board Of Trustees Of The University Of Illinois In vivo synthesis of connective tissues
CA2554994C (en) 2004-01-30 2015-05-19 Ferrosan A/S Haemostatic sprays and compositions
KR20070046093A (ko) 2004-07-09 2007-05-02 훼로산 아크티에 셀스카브 히알루론산을 포함하는 지혈 조성물
DE102004039537A1 (de) * 2004-08-13 2006-02-23 Phenion Gmbh & Co. Kg Vernetzte Kollagenmatrix zur Herstellung eines Hautäquivalentes
US7993738B2 (en) * 2004-11-30 2011-08-09 The Regents Of The University Of Michigan Modified porous materials and method of forming the same
WO2006107188A1 (en) 2005-04-06 2006-10-12 Fujifilm Manufacturing Europe B.V. A non-porous film for culturing cells
US8227415B2 (en) 2005-04-06 2012-07-24 Fujifilm Manufacturing Europe B.V. Non-porous film for culturing cells
JP2005305177A (ja) * 2005-05-11 2005-11-04 Toyobo Co Ltd 組織付属器官様構造体を含む人工組織およびその製造方法
US8709081B2 (en) 2006-10-23 2014-04-29 Stemedica Cell Technologies, Inc. Cellular scaffold
US8105380B2 (en) * 2006-10-23 2012-01-31 Stemedica Cell Technologies, Inc. Cellular scaffold
US20090004455A1 (en) * 2007-06-27 2009-01-01 Philippe Gravagna Reinforced composite implant
JP5252538B2 (ja) * 2007-11-09 2013-07-31 グンゼ株式会社 弾性線維組織を有する培養血管の製造方法及び弾性線維組織を有する培養血管
US9308068B2 (en) 2007-12-03 2016-04-12 Sofradim Production Implant for parastomal hernia
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ATE509095T1 (de) 2008-05-28 2011-05-15 Symrise Ag Menschliches ex-vivo-hautmodell
US9242026B2 (en) * 2008-06-27 2016-01-26 Sofradim Production Biosynthetic implant for soft tissue repair
ES2343721B1 (es) 2008-12-19 2011-06-06 Histocell, S.L. Sistema de transporte de celulas.
FR2949688B1 (fr) 2009-09-04 2012-08-24 Sofradim Production Tissu avec picots revetu d'une couche microporeuse bioresorbable
JP5889780B2 (ja) 2010-03-02 2016-03-22 富士フイルム株式会社 細胞支持体及び骨再生材
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AU2012313984B2 (en) 2011-09-30 2016-02-11 Covidien Lp Reversible stiffening of light weight mesh
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CN104159527B (zh) 2012-03-06 2017-04-12 弗罗桑医疗设备公司 包含止血糊剂的压力容器
FR2990106B1 (fr) * 2012-05-03 2014-05-09 Genoskin Systeme permettant la maintenance en survie et le transport de biospsies de peau et ses applications
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FR2994185B1 (fr) 2012-08-02 2015-07-31 Sofradim Production Procede de preparation d’une couche poreuse a base de chitosane
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WO2014049446A2 (en) 2012-09-28 2014-04-03 Sofradim Production Packaging for a hernia repair device
FR3006578B1 (fr) 2013-06-07 2015-05-29 Sofradim Production Prothese a base d’un textile pour voie laparoscopique
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EP3085337B1 (de) 2015-04-24 2022-09-14 Sofradim Production Prothese zur unterstützung einer bruststruktur
ES2676072T3 (es) 2015-06-19 2018-07-16 Sofradim Production Prótesis sintética que comprende un tejido de punto y una película no porosa y método para formarla
CA2986981A1 (en) 2015-07-03 2017-01-12 Ferrosan Medical Devices A/S Syringe for mixing two components and for retaining a vacuum in a storage condition
CN113201482A (zh) * 2015-09-25 2021-08-03 广东博溪生物科技有限公司 一种用于含微血管管腔的双层皮肤培养的培养液
EP3195830B1 (de) 2016-01-25 2020-11-18 Sofradim Production Prothese zur hernienreparatur
US20180100142A1 (en) * 2016-10-06 2018-04-12 Mattek Corporation Methods and Kits for Production of Tissue Equivalents from Cryopreserved Cells
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WO2019143498A1 (en) * 2018-01-22 2019-07-25 ClearIt, LLC Methods and compositions for simulation of the dermal compartment
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EP3653171B1 (de) 2018-11-16 2024-08-21 Sofradim Production Implantate zur weichgewebereparatur
CA3124411A1 (en) 2018-12-19 2020-06-25 ClearIt, LLC Systems and methods for tattoo removal using an applied electric field
WO2021149761A1 (ja) * 2020-01-22 2021-07-29 国立研究開発法人産業技術総合研究所 表層に角質層が形成された不死化皮膚細胞の３次元培養物、前記３次元培養物の製造方法、および前記３次元培養物を用いた被検物質の評価方法
US12064330B2 (en) 2020-04-28 2024-08-20 Covidien Lp Implantable prothesis for minimally invasive hernia repair
KR102212669B1 (ko) * 2020-04-29 2021-02-05 (주)바이오다인 알코올계 용액 조성물의 젤리화를 이용한 세포 검사 방법
KR102182077B1 (ko) * 2020-04-29 2020-11-23 (주)바이오다인 세포 고정용 알코올계 젤리 조성물
EP4677357A1 (de) * 2023-03-08 2026-01-14 Unilever IP Holdings B.V. Verfahren zum nachweis der wirkungsart eines hautpflegeproduktes oder einer komponente davon

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5263983A (en) * 1988-03-09 1993-11-23 Terumo Kabushiki Kaisha Medical material and prosthetic skin in which cells can invade
US5273900A (en) * 1987-04-28 1993-12-28 The Regents Of The University Of California Method and apparatus for preparing composite skin replacement
US5282859A (en) * 1990-04-24 1994-02-01 Mark Eisenberg Composite living skin equivalents

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4485096A (en) * 1982-02-26 1984-11-27 Massachusetts Institute Of Technology Tissue-equivalent and method for preparation thereof
US4837285A (en) * 1984-03-27 1989-06-06 Medimatrix Collagen matrix beads for soft tissue repair
US4835102A (en) * 1987-03-31 1989-05-30 Eugene Bell Tissue equivalent test systems
US5350583A (en) * 1988-03-09 1994-09-27 Terumo Kabushiki Kaisha Cell-penetrable medical material and artificial skin
US5024841A (en) * 1988-06-30 1991-06-18 Collagen Corporation Collagen wound healing matrices and process for their production
CA2119064A1 (en) * 1993-03-17 1994-09-18 Richard A. Berg Dermal-epidermal in vitro test system
JP2858087B2 (ja) * 1994-09-19 1999-02-17 グンゼ株式会社 組織培養用基材及び組織培養法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5273900A (en) * 1987-04-28 1993-12-28 The Regents Of The University Of California Method and apparatus for preparing composite skin replacement
US5263983A (en) * 1988-03-09 1993-11-23 Terumo Kabushiki Kaisha Medical material and prosthetic skin in which cells can invade
US5282859A (en) * 1990-04-24 1994-02-01 Mark Eisenberg Composite living skin equivalents

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NERMUT M V ET AL: "Method for the study of the underside of cultured cell monolayers.", EXPERIMENTAL CELL RESEARCH, (1991 JAN) 192 (1) 311-4., XP002123798 *

Also Published As

Publication number Publication date
JP2858087B2 (ja) 1999-02-17
DE69534869T2 (de) 2006-09-21
US6051425A (en) 2000-04-18
DE69535992D1 (de) 2009-09-24
DE69534869D1 (de) 2006-05-11
EP0702081A2 (de) 1996-03-20
EP0702081B1 (de) 2006-03-15
JPH0889239A (ja) 1996-04-09
EP1361265A1 (de) 2003-11-12
EP1361265B1 (de) 2009-08-12
US6300128B1 (en) 2001-10-09

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