EP0709132A1 - Dispositif de filtration de fluide - Google Patents

Dispositif de filtration de fluide Download PDF

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Publication number
EP0709132A1
EP0709132A1 EP94117112A EP94117112A EP0709132A1 EP 0709132 A1 EP0709132 A1 EP 0709132A1 EP 94117112 A EP94117112 A EP 94117112A EP 94117112 A EP94117112 A EP 94117112A EP 0709132 A1 EP0709132 A1 EP 0709132A1
Authority
EP
European Patent Office
Prior art keywords
test tube
tubular body
fluid
centrifuge
tube assembly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP94117112A
Other languages
German (de)
English (en)
Inventor
Giuseppe Cianci
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MEMBRANE SEPARATION TECHNOLOGIES Srl
Original Assignee
MEMBRANE SEPARATION TECHNOLOGIES Srl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MEMBRANE SEPARATION TECHNOLOGIES Srl filed Critical MEMBRANE SEPARATION TECHNOLOGIES Srl
Priority to EP94117112A priority Critical patent/EP0709132A1/fr
Publication of EP0709132A1 publication Critical patent/EP0709132A1/fr
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes

Definitions

  • the present invention relates to a fluid filtration device particularly useful for laboratory analysis, and in particular to a test tube assembly containing a fluid to be separated by means of a centrifuge.
  • filtration devices comprise a centrifuge, and a test tube assembly presenting a first tube with a filtration membrane and into which the fluid to be filtered is introduced, and a second tube into which part of the test fluid is filtered by centrifugal action.
  • known filtration devices provide for concentrating inside the second tube the fluid portion of a smaller molecular size than the pores of the membrane, which portion is then subjected to laboratory analysis. For analyzing different molecular size portions of the same fluid, this must be filtered twice using two test tube assemblies fitted, naturally, with membranes of different filtration grades. That is, when once filtered, the concentrated portion in the second tube of the first assembly must be poured into the first tube of the second assembly and the centrifuge operated once more.
  • a fluid filtration device presenting a centrifuge; a test tube assembly; and a mechanical arm supporting the test tube assembly; characterized in that said test tube assembly comprises a number of successive chambers, each separated from the adjacent chamber by a respective filtration membrane, so that the fluid introduced into the top said chamber is cascade filtered repeatedly during operation of the centrifuge.
  • Number 1 in Figure 1 indicates a filtration device comprising a known centrifuge 2 (therefore shown only schematically), and a test tube assembly 3 presenting a number of successive chambers, each separated from the adjacent chamber by a filtration membrane.
  • test tube assembly 3 provides for performing two filtrations and therefore comprises three successive test tubes 4, 5 and 6.
  • the fluid to be filtered is introduced into tube 4; assembly 3 is fitted to a mechanical arm (not shown) of centrifuge 2; and centrifuge 2 is operated to separate the fluid portion of a molecular size smaller than the diameter of the pores of the membrane between tubes 4 and 5, and to separate, from the fluid filtered into tube 5, the fluid portion of a molecular size smaller than the diameter of the pores of the membrane between tubes 5 and 6.
  • test tube 4 comprises a cylindrical body 7 made of transparent plastic material and presenting a top end 8 with an inside diameter equal to the outside diameter of the rest of the body, so as to define an inner annular shoulder 11.
  • body 7 presents an inner annular projection 12; and test tube 4 also comprises an upside down cup-shaped body 13 presenting a base wall 14, a cylindrical lateral wall 15, and an annular flange 16 extending outwards from the bottom edge of wall 15.
  • Body 13 is made of transparent plastic material and fitted inside the bottom end of body 7, with the exception of flange 16 which contacts the bottom edge of body 7 and presents an outside diameter equal to that of body 7.
  • Test tube 4 also comprises a circular filtration membrane 17 fitted between the upper face of wall 14 of body 13 and projection 12 of body 7. More specifically, the periphery of membrane 17 is pressed against projection 12 by the periphery of wall 14, which wall 14 presents a number of holes 18 for permitting the fluid filtered through membrane 17 to flow into test tube 5.
  • membrane 17 and body 13 are made, e.g. welded, integral with each other and with the bottom end of body 7.
  • test tube 5 is identical to tube 4 - and therefore illustrated using the same component numbering system - except that filtration membrane 17 of tube 5 presents smaller-diameter pores than that of tube 4.
  • the bottom end of tube 4 is fitted inside the seat defined by top end 8 of tube 5, and flange 16 of body 13 of tube 4 rests on shoulder 11 of body 7 of tube 5.
  • test tube 6 comprises a cylindrical body 21 made of transparent plastic material and presenting a base wall 22, and a top end 23 with an inside diameter equal to the outside diameter of the rest of the body, so as to define an inner annular shoulder 24.
  • End 23 of tube 6 is identical to ends 8 of tubes 4 and 5, so as to receive the bottom end of tube 5.
  • test tube 4 presents a fluidtight cap 25 with a tubular stem 26 inserted inside the seat defined by top end 8 of body 7.
  • test tubes of the type indicated by 4 and 5 are available, each with a membrane 17 of a given filtration grade.
  • the appropriate test tubes are selected and fitted together; and, after assembling test tube 6 into which the smallest molecular size fluid portion is eventually centrifuged, the fluid to be filtered is introduced into the first test tube.
  • test tubes 4, 5 and 6 are all provided with a cap 25 which is removed for assembling the tubes, that of tube 4 naturally being replaced after introducing the fluid.
  • centrifuging comprises fitting test tube assembly 3, at an inclined angle in relation to a vertical axis of rotation, to a mechanical arm (not shown) of centrifuge 2.
  • a mechanical arm (not shown) of centrifuge 2.
  • centrifugal force is applied to the fluid inside test tube 4, which force produces such a pressure as to filter the fluid, so that the fluid portion of a molecular size smaller than the diameter of the pores of the first membrane 17 is fed into test tube 5.
  • test tube 5 receives said fluid portion, this too is subjected to centrifugal force, so that a further portion of said portion of fluid is filtered into test tube 6.
  • each test tube is located a different distance from the center of rotation of centrifuge 2. More specifically, the distance of test tube 5 is greater, as shown in Figure 1 wherein A and B indicate the segments between the center of rotation of centrifuge 2 and the barycenter of test tubes 4 and 5.
  • a and B indicate the segments between the center of rotation of centrifuge 2 and the barycenter of test tubes 4 and 5.
  • the inclined angle of test tube assembly 3 assists cascade filtration by increasing the distance of test tube 5 and so subjecting the fluid inside tube 5 to greater centrifugal force as compared with test tube 4.
  • the filtration device may be fitted with microfiltration membranes (of any molecular size), in particular inorganic, e.g. ceramic, membranes; organic, e.g. polymer, membranes; or affinity membranes. It may be fitted with ultrafiltration, nanofiltration or reverse osmosis membranes (of any molecular size), in particular inorganic, e.g. ceramic, membranes; organic, e.g. polymer, membranes; membranes resistant to organic solvents; liquid membranes; or affinity membranes.
  • microfiltration membranes of any molecular size
  • inorganic e.g. ceramic, membranes
  • organic, e.g. polymer, membranes e.g. polymer, membranes
  • affinity membranes of any molecular size
  • it may be used for purifying the fluid by providing at least one membrane-fitted test tube with a predetermined quantity of chromatographic resin or gel (filtration, ion exchange, affinity or liquid resins), so as to obtain, after centrifuging, a purified or waste product accordingly.
  • chromatographic resin or gel filtration, ion exchange, affinity or liquid resins
  • test tube assembly 3 which is therefore extremely easy to assemble.
  • test tube assembly may comprise a greater number of test tubes for performing more than the two filtrations provided for by the assembly described and illustrated herein; one of the test tubes may be in the form of an inverted Y with respective membranes, even of different filtration grades, along the two branch portions; and changes may be made to the manner in which the membrane and membrane supporting body are fitted to the test tube body.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
EP94117112A 1994-10-28 1994-10-28 Dispositif de filtration de fluide Withdrawn EP0709132A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP94117112A EP0709132A1 (fr) 1994-10-28 1994-10-28 Dispositif de filtration de fluide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP94117112A EP0709132A1 (fr) 1994-10-28 1994-10-28 Dispositif de filtration de fluide

Publications (1)

Publication Number Publication Date
EP0709132A1 true EP0709132A1 (fr) 1996-05-01

Family

ID=8216418

Family Applications (1)

Application Number Title Priority Date Filing Date
EP94117112A Withdrawn EP0709132A1 (fr) 1994-10-28 1994-10-28 Dispositif de filtration de fluide

Country Status (1)

Country Link
EP (1) EP0709132A1 (fr)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0891742A3 (fr) * 1997-07-14 2000-01-12 Becton, Dickinson and Company Eprouvette collecteur de liquide corporel à capacité réduite
EP0901823A3 (fr) * 1997-09-12 2000-01-19 Becton, Dickinson and Company Dispositif avec récipient de prélèvement
GB2360715A (en) * 2000-03-30 2001-10-03 Formulations Pro Membrane based molecule separator
US7132531B1 (en) 1999-04-28 2006-11-07 Avecia Limited Reactor
EP2260943A1 (fr) * 2009-06-11 2010-12-15 Innosieve Diagnostics B.V. Tuyau centrifuge microfiltre
EP2467696A4 (fr) * 2009-07-27 2016-11-02 Thailand Res Fund Appareil et procédé d analyse de particules
CN111801148A (zh) * 2018-03-19 2020-10-20 株式会社村田制作所 过滤装置
CN115463438A (zh) * 2022-09-16 2022-12-13 河北科技大学 自动蒸馏萃取一体机及自动蒸馏萃取方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3300051A (en) * 1963-09-26 1967-01-24 Internat Equipment Co Filter tube for use in a centrifuge
JPS5840154A (ja) * 1981-09-03 1983-03-09 Michizo Yamano 円錐状メンブランフイルタ−を設置した試験管
US4636361A (en) * 1985-11-18 1987-01-13 Miklos Marian Device for separating liquid fractions
WO1991007648A1 (fr) * 1989-11-08 1991-05-30 Fmc Corporation Tube centrifuge combine et moyen de selection poreux pour la separation et la recuperation de matieres biologiques

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3300051A (en) * 1963-09-26 1967-01-24 Internat Equipment Co Filter tube for use in a centrifuge
JPS5840154A (ja) * 1981-09-03 1983-03-09 Michizo Yamano 円錐状メンブランフイルタ−を設置した試験管
US4636361A (en) * 1985-11-18 1987-01-13 Miklos Marian Device for separating liquid fractions
WO1991007648A1 (fr) * 1989-11-08 1991-05-30 Fmc Corporation Tube centrifuge combine et moyen de selection poreux pour la separation et la recuperation de matieres biologiques

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN vol. 7, no. 122 (C - 168) 26 May 1983 (1983-05-26) *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0891742A3 (fr) * 1997-07-14 2000-01-12 Becton, Dickinson and Company Eprouvette collecteur de liquide corporel à capacité réduite
EP0901823A3 (fr) * 1997-09-12 2000-01-19 Becton, Dickinson and Company Dispositif avec récipient de prélèvement
AU748070B2 (en) * 1997-09-12 2002-05-30 Becton Dickinson & Company Collection container assembly
US7132531B1 (en) 1999-04-28 2006-11-07 Avecia Limited Reactor
GB2360715A (en) * 2000-03-30 2001-10-03 Formulations Pro Membrane based molecule separator
GB2360715B (en) * 2000-03-30 2004-02-18 Formulations Pro Molecule separation device and method combining multiple filtration media
EP2260943A1 (fr) * 2009-06-11 2010-12-15 Innosieve Diagnostics B.V. Tuyau centrifuge microfiltre
WO2010143954A1 (fr) * 2009-06-11 2010-12-16 Innosieve Diagnostics B.V. Tube de centrifugation à microfiltre
EP2467696A4 (fr) * 2009-07-27 2016-11-02 Thailand Res Fund Appareil et procédé d analyse de particules
CN111801148A (zh) * 2018-03-19 2020-10-20 株式会社村田制作所 过滤装置
CN111801148B (zh) * 2018-03-19 2022-12-02 株式会社村田制作所 过滤装置
CN115463438A (zh) * 2022-09-16 2022-12-13 河北科技大学 自动蒸馏萃取一体机及自动蒸馏萃取方法

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