EP0805865A1 - Acide desoxyribonucleique codant pour la glutathion-s-transferase et son utilisation - Google Patents

Acide desoxyribonucleique codant pour la glutathion-s-transferase et son utilisation

Info

Publication number
EP0805865A1
EP0805865A1 EP96900929A EP96900929A EP0805865A1 EP 0805865 A1 EP0805865 A1 EP 0805865A1 EP 96900929 A EP96900929 A EP 96900929A EP 96900929 A EP96900929 A EP 96900929A EP 0805865 A1 EP0805865 A1 EP 0805865A1
Authority
EP
European Patent Office
Prior art keywords
dna
gstiiic
plants
transgenic plants
dna according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP96900929A
Other languages
German (de)
English (en)
Inventor
Barbara Bieseler
Peter Reinemer
Rüdiger Hain
Karlheinz Mann
Hans-Jörg REIF
Jürgen Ernst THOMZIK
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience AG
Original Assignee
Bayer AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer AG filed Critical Bayer AG
Publication of EP0805865A1 publication Critical patent/EP0805865A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1088—Glutathione transferase (2.5.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance

Definitions

  • GSTIIIc glutathione-S-transferase IIIc
  • plants in whose genome the new GSTIIIc DNA was incorporated have an increased resistance to herbicides, preferably heteroaryloxyacetamide herbicides, in comparison with the corresponding “starting plants”
  • the GSTIIIc DNA as Xbal / BamHI fragment was purified from the vector pET3a-GSTIIIc and cloned into the plasmid Bluescript-SKII (Xbal / BamHI-linearized; Stratagene).
  • the GSTIIIc DNA was then isolated from the plasmid Bluescript-SKII-GSTIIIc obtained in this way via an Sstl / Smal restriction cleavage and ligated into the vector pRTlOl (Sstl / Smal-linerized; Töpfer et al. 1987).
  • the Escherichia coli strain pET3a-GSTIIIc was obtained from the German Collection of Microorganisms (DSM), Mascheroder Weg lb, D-38124 Braunschweig, Federal Republic of Germany in accordance with the provisions of the Treaty of Budapest on the international recognition of the deposit of microorganisms for the Purposes of patent procedures deposited (Filing date: January 17, 1995).
  • the strain received the deposit number DSM 9677.
  • Buffer B 1.0 M potassium phosphate pH 7.3
  • Gradient 0-100% B in 500 ml
  • I I ⁇ m reaction mixture (bulk reaction mix) consisting of:
  • the mRNA is removed by alkaline hydrolysis.
  • the synthesized cDNA is precipitated and used as a template for the polymerase chain reaction.
  • the shoot cultures are kept in a culture room at 24-26 ° C under 12 h light (1000-3000 lux).
  • leaf protoplasts approx. 2 g of leaves (approx. 3-5 cm long) are cut into small pieces (0.5 cm x 1 cm) with a fresh razor blade.
  • the leaf material is in 20 ml enzyme solution consisting of K3
  • the GSTIIIc DNA was then purified as an EcoRI / Smal fragment from the vector pRTl01 -GSTIIIc and linearized into the expression vector pSS (EcoRI / Smal, cloned (Voss A et al, Molec. Breeding 1: 15-26 (1995)) .
  • the Zeil suspension is at a density of 5 x 10 4 / ml in K3 medium (0.3 m sucrose) with 1 mg / 1 NAA (naphthyl-1-acetic acid), 0.2 mg / 1 kinetin and 500 mg / 1 of the cephalosporin antibiotic cefotaxime.
  • K3 medium 0.3 m sucrose
  • NAA naphthyl-1-acetic acid
  • kinetin 500 mg / 1 of the cephalosporin antibiotic cefotaxime.
  • the cell suspension is diluted every week with fresh K3 medium and the osmotic value of the medium is gradually increased by 0.05 M sucrose (approx. 60 mOsm / kg) per Week reduced.
  • a modified "bead type culture” technique (Shillito et al. 1983) is used for the culture and selection of kanamycin-resistant colonies described below.
  • K3 medium 0.3 M sucrose + hormones; 1.2% (Seaplaque) LMT agarose (low melting agarose, marine Colloids) are mixed in 5 cm Petri dishes, for this purpose agarose is dry autoclaved and briefly boiled in the microwave after adding K3 medium.
  • T L -DNA gene 5 controls the tissue- specific expression of chimaeric genes carried by a noval type of Agrobacterium linary vector. Mol. Gen. Genet. (1986) 204: 338-396
  • the plants are grown at a temperature of 23 ° C and a relative humidity of 70 - 80%.
  • the treatment with the herbicidal compound mentioned above, formulated as 70 WP (wettable powder), takes place 24 hours after laying out the seeds at concentrations which correspond to an application rate of 2,000-4,000 g of active ingredient / ha.
  • This international depository accepts the microorganism designated under I, which it received on 19 95 - 01 - 17 (date of first deposit) 1 .

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne un nouvel acide désoxyribonucléique (ADN) et son utilisation pour la transformation de vecteurs, d'organismes hôtes et de végétaux ainsi que pour la production de végétaux présentant une résistance élevée aux herbicides.
EP96900929A 1995-01-23 1996-01-10 Acide desoxyribonucleique codant pour la glutathion-s-transferase et son utilisation Withdrawn EP0805865A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19501840 1995-01-23
DE19501840A DE19501840A1 (de) 1995-01-23 1995-01-23 Desoxyribonukleinsäure und ihre Verwendung
PCT/EP1996/000068 WO1996023072A1 (fr) 1995-01-23 1996-01-10 Acide desoxyribonucleique codant pour la glutathion-s-transferase et son utilisation

Publications (1)

Publication Number Publication Date
EP0805865A1 true EP0805865A1 (fr) 1997-11-12

Family

ID=7752046

Family Applications (1)

Application Number Title Priority Date Filing Date
EP96900929A Withdrawn EP0805865A1 (fr) 1995-01-23 1996-01-10 Acide desoxyribonucleique codant pour la glutathion-s-transferase et son utilisation

Country Status (11)

Country Link
US (1) US5968796A (fr)
EP (1) EP0805865A1 (fr)
JP (1) JPH10512451A (fr)
CN (1) CN1169160A (fr)
AU (1) AU4484996A (fr)
BR (1) BR9606780A (fr)
CA (1) CA2210901A1 (fr)
DE (1) DE19501840A1 (fr)
RU (1) RU2169196C2 (fr)
UA (1) UA51642C2 (fr)
WO (1) WO1996023072A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6063570A (en) 1997-09-05 2000-05-16 E. I. Du Pont De Nemours And Company Soybean glutathione-S-transferase enzymes
US6096504A (en) * 1997-09-05 2000-08-01 E. I. Du Pont Nemours And Company Maize glutathione-S-transferase enzymes
US6168954B1 (en) 1997-09-05 2001-01-02 E. I. Du Pont De Nemours & Company Soybean glutathione-S-transferase enzymes
WO2000018937A1 (fr) * 1998-09-30 2000-04-06 E.I. Du Pont De Nemours And Company Enzymes de glutathione-s-transferase issues du mais
CA2340791A1 (fr) * 1998-09-30 2000-04-06 E.I. Du Pont De Nemours And Company Enzymes de glutathione-s-transferase issues du soja
AU2924800A (en) * 1999-03-03 2000-09-21 Syngenta Limited Use of glutathione-s-transferase to increase stress tolerance in plants
GT200400123AA (es) * 2003-06-20 2009-07-10 Modulacion de los niveles de un precursor de saborizante de cafe en los granos de cafe verde (solicitud fraccionaria)
CN103194466B (zh) * 2013-04-24 2014-05-07 昆明理工大学 一种岷江百合谷胱甘肽S-转移酶基因LrGSTU5及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA20977A1 (fr) * 1986-05-19 1987-12-31 Ciba Geigy Ag Plantes tolerant les herbicides contenant le gene de gluthathione S-Transferase
US5714365A (en) * 1990-07-20 1998-02-03 Roussel Uclaf Sucrose phosphate synthetase isolated from maize
GB9114259D0 (en) * 1991-07-02 1991-08-21 Ici Plc Plant derived enzyme and dna sequences

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9623072A1 *

Also Published As

Publication number Publication date
CN1169160A (zh) 1997-12-31
BR9606780A (pt) 1997-12-30
DE19501840A1 (de) 1996-07-25
CA2210901A1 (fr) 1996-08-01
UA51642C2 (uk) 2002-12-16
RU2169196C2 (ru) 2001-06-20
US5968796A (en) 1999-10-19
AU4484996A (en) 1996-08-14
WO1996023072A1 (fr) 1996-08-01
JPH10512451A (ja) 1998-12-02

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