EP1070963A2 - Bac de rinçage - Google Patents

Bac de rinçage Download PDF

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Publication number
EP1070963A2
EP1070963A2 EP00112838A EP00112838A EP1070963A2 EP 1070963 A2 EP1070963 A2 EP 1070963A2 EP 00112838 A EP00112838 A EP 00112838A EP 00112838 A EP00112838 A EP 00112838A EP 1070963 A2 EP1070963 A2 EP 1070963A2
Authority
EP
European Patent Office
Prior art keywords
tub
trough
flushing
holes
pipette tips
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP00112838A
Other languages
German (de)
English (en)
Other versions
EP1070963A3 (fr
EP1070963B1 (fr
Inventor
Peter Zimmermann
Uwe Naumann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cybio AG
Original Assignee
Cybio Instruments GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cybio Instruments GmbH filed Critical Cybio Instruments GmbH
Publication of EP1070963A2 publication Critical patent/EP1070963A2/fr
Publication of EP1070963A3 publication Critical patent/EP1070963A3/fr
Application granted granted Critical
Publication of EP1070963B1 publication Critical patent/EP1070963B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/04—Cleaning involving contact with liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L13/00—Cleaning or rinsing apparatus
    • B01L13/02—Cleaning or rinsing apparatus for receptacle or instruments
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips

Definitions

  • the invention relates to a rinsing tub system for cleaning the pipette tips of Multipipettors or the transfer needles of replication systems with a matrix arranged pipette tips or transfer needles.
  • Muitipipettors are used in particular in chemical and biochemical drug research to test a large variety of substances (substance libraries) for their effectiveness for an intended use (HTS: high throughput screening).
  • the highest throughputs are achieved in particular with multipipettors with pipette tips arranged in a matrix.
  • the commercially available multi-pipettors from Robbins Scientific Sunnyvale, CA and OpalJena GmbH (DD-PS 260571) may be mentioned as an example.
  • MTP microtitration plate
  • test substance transfer has also increased Replication systems used, such as those from V&P Scientific Inc., CA are offered.
  • Replication systems consist of two-dimensionally arranged transfer needles of the same diameter and are also used for test substance transfer in HTS applications.
  • the transfer needles are advantageously designed such that that when immersed in a test substance only on its face meniscus-like test substance.
  • the volume of the transferable Substance essentially due to the diameter of the transfer needles used determined and can be significantly less than with the above Multipipettors is possible.
  • a common requirement for both multipipettors and Replication systems is after cleaning the pipette tips or transfer needle after a cycle of transfer of substances to contaminate the Test substance for the new admission or a crosstalk in the follow-up cycle and thus prevent the results from being falsified in subsequent cycles.
  • the pipette tips are cleaned by in a first container of clean rinsing liquid and in an adjacent one arranged second vessel is ejected.
  • a disadvantage of such The solution is, on the one hand, that the two vessels alternate under the pipette tips must be arranged or the pipette tips guided over the tubes and on the other hand through the outer surface of the pipette tips immersion in the essentially flow-free rinsing liquid only insufficient cleaning, which also contaminates the clean Rinsing liquid with test substance leads.
  • the first-mentioned disadvantage is solved with a flushing tub system for a multipipettor, according to DE 196 35 004 C1.
  • a washing tub system consists of a first tub for receiving the unused (clean) washing liquid and a second tub for receiving the contaminated washing liquid, the first tub being arranged on the second tub and having webs with through openings (through holes) in the floor distributed over its bottom Grid dimensions of a matrix-shaped multipipettor are available.
  • the first tub is filled with the clean rinsing liquid via an inlet.
  • An overflow or a fill level sensor is provided so that the fill level of the first tub does not exceed the web height and can thus run through the through holes into the second tub.
  • the object of the invention is to provide a flushing tub system in the preamble of To create claim 1 type, with which it is possible to form a matrix arranged pipette tips or transfer needles effectively without residue clean and perform the rinsing process more effectively.
  • a washing tub system according to the preamble of claim 1 in that the lower tub for continuous filling with washing liquid has at least one inflow and at least one outlet is present on the upper tub.
  • the flushing tub system according to the invention is not a trivial kinematic reversal, since a completely different effect is achieved here by a different arrangement of the means known per se and thus such a flushing tub system has different features than those known from the prior art.
  • Essential to the invention is the continuous filling of the lower trough with clean washing liquid, which is conveyed through the through holes into the upper trough and discharged from there. The pipette tips or transfer needles are inserted into the through holes for cleaning.
  • the flow of the clean rinsing liquid flows around the outer surface of the pipette tips or transfer needles due to the flow generated in the through-holes and thus cleans them.
  • the residues of test substance that are released in the process are conveyed upward in the through holes by the flow, generated by the continuous supply of clean rinsing liquid, and are rinsed over the edge thereof.
  • the decisive advantage over the prior art is the contamination of the container for the clean rinsing liquid, which, in the expanded sense, includes the conversions of the through holes that protrude into the upper trough as well as the contamination of those located therein clean rinsing liquid, safely avoided.
  • the safe avoidance of contamination of the through hole conversions is important not only to prevent the contamination of the clean liquid rising therein, but also to prevent the pipette tips or transfer needles from being reintroduced when the rinsing process is repeated, and not again or additionally Contamination of the conversions. Since the pipette tips or transfer needles are immersed in the through holes for cleaning, in contrast to the prior art, their conversions need only protrude slightly into the interior of the upper tub 1, as a result of which they form elevations of low height.
  • the edge area around the through holes should either be very narrow, or a flat surface inclined to the trough floor of the upper trough represent.
  • the first exemplary embodiment shown in FIGS. 1 and 2 essentially consists of an upper trough 1.1, a lower trough 2.1, an intermediate trough 3 and a multiplicity of tubes 4 of the same dimensions, which are arranged in a matrix, with one end in the intermediate trough 3 are attached.
  • the free ends of the tubes 4 protrude through openings 5, which have a larger diameter than the outer diameter of the tubes 4, perpendicular to the tub bottom into the interior of the upper tub 1.1.
  • the fixed end of the tube 4 is connected via a channel 6 to the lower trough 2.1.
  • the lower tub 2.1 is continuously filled with clean washing liquid.
  • the rinsing liquid rises up through the channels 6 in the tube 4 and flows over the edge thereof, in order to then flow into the intermediate trough 3 through the free area of the openings 5. From here, the rinsing liquid can drain through the outlet 9 due to gravity or can be removed by pumping.
  • the inside diameters of channels 6 are chosen to be significantly smaller than the inside diameters of tubes 4. So that the rising washing liquid can leave the tubes 4 unhindered, tubes 4 with a small wall thickness are used. The wall thickness is decisive for the edge surface, which the rinsing liquid must overflow in the horizontal direction.
  • the cleaning process for the pipette tips or transfer needles generally consists of repeating the rinsing process.
  • the rinsing process begins by positioning the rinsing system and the matrix-like arrangement of pipette tips or transfer needles in relation to one another such that the individual pipette tips or transfer needles are each aligned with a tube 4 above it.
  • By lifting the flushing system or lowering the pipette tips or transfer needles they are immersed in the tubes 4.
  • the clean rinsing liquid flows around the outer surface of the pipette tips or transfer needle due to the flow generated in the tubes 4 and thus cleans them.
  • the interior of the pipette is simultaneously filled with clean rinsing liquid while it is being rinsed around.
  • the pipette tips are raised again or the flushing system is lowered.
  • a horizontal relative movement follows, preferably by half the distance between two adjacent tubes 4, and the pipette contents are dispensed into the upper tub 1.1.
  • the rinsing process can be repeated any number of times without a complete exchange of the clean rinsing liquid being necessary.
  • the effectiveness of cleaning for the outer surface of the pipette tips or transfer needle depends solely on the duration of the flow through the clean rinsing liquid and its flow rate. The number of cycles of suction and expulsion, ie the repeated repetition of the rinsing process, is decisive for the effectiveness of cleaning the inner surface of the pipette tips.
  • FIGS. 3 and 4 A technologically simpler embodiment of a flushing tub system according to the invention is described in a second exemplary embodiment with reference to FIGS. 3 and 4.
  • the second exemplary embodiment consists of an upper trough 1.2 and a lower trough 2.2, the bottom of the upper trough 1.2 being designed in the form of webs 7 arranged next to one another with through bores 8.
  • the lower trough 2.2 is continuously filled via an inlet 10 with clean washing liquid.
  • the rinsing liquid rises above the through holes 8 in the webs 7 and flows over the edge thereof into the interior of the upper tub 1.2. From here the rinsing liquid can be pumped out via an outlet 9. It is also important here that the through bores 8 are arranged in the same pitch as the pipette tips or transfer needles.
  • the edge region of the webs 7 around the through bores should be designed as a flat surface inclined towards the bottom of the upper trough 1.2, as shown in FIG. 4 .
  • the upper trough 1.2 is advantageously made of a hydrophobic material or coated with such a material so that a coherent liquid level is formed in it and thus a uniform suction of the contaminated rinsing liquid is made possible.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Cleaning By Liquid Or Steam (AREA)
  • Cleaning In General (AREA)
EP00112838A 1999-07-19 2000-06-17 Bac de rinçage Expired - Lifetime EP1070963B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19934090A DE19934090A1 (de) 1999-07-19 1999-07-19 Spülwannensystem
DE19934090 1999-07-19

Publications (3)

Publication Number Publication Date
EP1070963A2 true EP1070963A2 (fr) 2001-01-24
EP1070963A3 EP1070963A3 (fr) 2002-09-18
EP1070963B1 EP1070963B1 (fr) 2008-11-19

Family

ID=7915476

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00112838A Expired - Lifetime EP1070963B1 (fr) 1999-07-19 2000-06-17 Bac de rinçage

Country Status (4)

Country Link
US (1) US6475444B1 (fr)
EP (1) EP1070963B1 (fr)
JP (1) JP2001054772A (fr)
DE (2) DE19934090A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1262306A1 (fr) * 2001-05-23 2002-12-04 Novartis AG Système et procédé pour traiter des objets, comme des lentilles ophtalmiques, lentilles de contact ou des dispositifs medicaux avec un fluide

Families Citing this family (19)

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Publication number Priority date Publication date Assignee Title
US7189365B1 (en) * 1999-03-04 2007-03-13 Riken Liquid treating equipment including a storage vessel and a discharge vessel
US20050106621A1 (en) * 2000-08-16 2005-05-19 Winegarden Neil A. Devices and methods for producing microarrays of biological samples
US6902702B1 (en) * 2000-08-16 2005-06-07 University Health Network Devices and methods for producing microarrays of biological samples
US6884396B2 (en) * 2001-03-22 2005-04-26 Thomas W. Astle Pipettor reservoir for particulate-containing liquids
US7754609B1 (en) 2003-10-28 2010-07-13 Applied Materials, Inc. Cleaning processes for silicon carbide materials
JP2008107318A (ja) * 2006-09-27 2008-05-08 Fujifilm Corp 液循環装置、及び、測定装置
US8268238B2 (en) 2006-09-29 2012-09-18 Tyco Healthcare Group Lp System and method for recycling sterilant gas
US7666369B2 (en) 2006-09-29 2010-02-23 Tyco Healthcare Group Lp System and method for recycling sterilant gas
KR101432161B1 (ko) * 2006-11-01 2014-08-20 퀀텀 글로벌 테크놀로지스, 엘엘씨 챔버 부품을 세정하기 위한 방법 및 장치
EP2205365B1 (fr) * 2007-10-17 2017-02-22 Aushon Biosystems Dispositif de lavage de tige à écoulement continu
CN201419170Y (zh) * 2009-03-18 2010-03-10 鸿富锦精密工业（深圳）有限公司 吸嘴清洗装置
DE102012106675B3 (de) * 2012-07-23 2013-10-17 Cybio Ag Absaugvorrichtung für eine matrixförmige Anordnung von Pipettenspitzen
CN102950075B (zh) * 2012-11-19 2015-09-16 苏州农业职业技术学院 喷水量均衡的洗瓶机喷针装置
ES2715391T3 (es) * 2013-05-01 2019-06-04 Douglas Scient Llc Lavado de pipetas
WO2017011243A1 (fr) * 2015-07-10 2017-01-19 Grenova, Llc Dispositif et procédé de lavage de pointe de pipette et système et procédé de distribution de fluide ou de semi-fluide associés
CN109070148B (zh) * 2016-05-11 2022-02-01 西门子医疗保健诊断公司 用于分析仪器的探针清洗站
KR102572150B1 (ko) * 2021-10-19 2023-08-28 송율아 초음파 세척이 가능한 초경봉 지지용 트레이
DE102024101291B4 (de) * 2023-01-20 2025-08-14 Miele & Cie. Kg Reinigungseinrichtung
DE102023123455B3 (de) * 2023-08-31 2025-01-16 Singulus Technologies Aktiengesellschaft Anlagemodul zum Sammeln und Entnehmen von Ausschuss

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JPH01209372A (ja) * 1988-02-18 1989-08-23 Toshiba Corp 自動化学分析装置の洗浄装置
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CH687592A5 (de) * 1993-10-18 1997-01-15 Eidgenoess Munitionsfab Thun Mehrgefaessanordnung zur Instrumental-Analyse.
US5916526A (en) * 1995-08-11 1999-06-29 Robbins Scientific Corporation Compartmentalized multi-well container
US5888830A (en) * 1995-09-22 1999-03-30 Berlex Laboratories, Inc. Apparatus and process for multiple chemical reactions
US5759494A (en) * 1995-10-05 1998-06-02 Corning Incorporated Microplates which prevent optical cross-talk between wells
DE19635004C1 (de) 1996-08-30 1997-11-20 Opaljena Ges Fuer Optische Ana Spülwannensystem für einen Multipipettor
US6054100A (en) * 1996-11-18 2000-04-25 Robbins Scientific Corporation Apparatus for multi-well microscale synthesis
US5961925A (en) * 1997-09-22 1999-10-05 Bristol-Myers Squibb Company Apparatus for synthesis of multiple organic compounds with pinch valve block
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1262306A1 (fr) * 2001-05-23 2002-12-04 Novartis AG Système et procédé pour traiter des objets, comme des lentilles ophtalmiques, lentilles de contact ou des dispositifs medicaux avec un fluide
US6884457B2 (en) 2001-05-23 2005-04-26 Novartis Ag System and method for treating articles with fluids
US7163583B2 (en) * 2001-05-23 2007-01-16 Novartis Ag System and method for treating articles with fluids

Also Published As

Publication number Publication date
EP1070963A3 (fr) 2002-09-18
DE19934090A1 (de) 2001-02-08
JP2001054772A (ja) 2001-02-27
US6475444B1 (en) 2002-11-05
DE50015451D1 (de) 2009-01-02
EP1070963B1 (fr) 2008-11-19

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