EP1070963B1 - Bac de rinçage - Google Patents

Bac de rinçage Download PDF

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Publication number
EP1070963B1
EP1070963B1 EP00112838A EP00112838A EP1070963B1 EP 1070963 B1 EP1070963 B1 EP 1070963B1 EP 00112838 A EP00112838 A EP 00112838A EP 00112838 A EP00112838 A EP 00112838A EP 1070963 B1 EP1070963 B1 EP 1070963B1
Authority
EP
European Patent Office
Prior art keywords
tray
rinsing
pipette tips
holes
upper tray
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP00112838A
Other languages
German (de)
English (en)
Other versions
EP1070963A3 (fr
EP1070963A2 (fr
Inventor
Peter Zimmermann
Uwe Naumann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cybio AG
Original Assignee
Cybio AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cybio AG filed Critical Cybio AG
Publication of EP1070963A2 publication Critical patent/EP1070963A2/fr
Publication of EP1070963A3 publication Critical patent/EP1070963A3/fr
Application granted granted Critical
Publication of EP1070963B1 publication Critical patent/EP1070963B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/04—Cleaning involving contact with liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L13/00—Cleaning or rinsing apparatus
    • B01L13/02—Cleaning or rinsing apparatus for receptacle or instruments
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips

Definitions

  • the invention relates to an apparatus and a method for cleaning the pipette tips of multipipettors or the transfer needles of replication systems with matrix-shaped pipette tips or transfer needles.
  • the device consists of a pump and a Spülwannensystem, as it is basically from the DE 196 35 004 C1 is known.
  • MTP microtitration plate
  • Replication systems such as those offered by V & P Scientific Inc., CA, have increasingly been used for test substance transfer for some time.
  • Replication systems consist of two-dimensionally arranged transfer needles of the same diameter and are also used for test substance transfer in HTS applications.
  • the transfer needles are advantageously designed in such a way that, when immersed in a test substance, test substance is deposited on the meniscus-shaped surface exclusively on its end face.
  • the volume of the transferable substance is determined essentially by the diameter of the transfer needles used and can be substantially lower than is possible with the above-mentioned multipipettors.
  • a common requirement for both multipipettors and replication systems is the cleaning of the pipette tips after one cycle of transfer of substances, contamination of the test substance upon resumption or crosstalk in the subsequent cycle and thus the falsification of the results in subsequent cycles to prevent.
  • the cleaning of the pipette tips takes place by picking up clean rinsing liquid from a first vessel and ejecting it into an adjacently arranged second vessel.
  • the disadvantage of such a solution is, on the one hand, that the two vessels must be arranged alternately under the pipette tips or the pipette tips must be guided over the vessels and, on the other, the outer surface of the pipette tips by immersion in the substantially flow-free flushing liquid only inadequately is cleaned, which also leads to contamination of the clean rinse with test substance.
  • the first-mentioned disadvantage is achieved with a Spülpannensystem for a Multipipettor, according to DE 196 35 004 C1 solved.
  • a Spülwannensystem consists of a first tray for receiving the unconsumed (clean) rinsing liquid and a second tray for receiving the contaminated rinsing liquid, wherein the first tray is arranged to sit on the second tray and distributed over its bottom webs with through holes (through holes) in Pitch of a matrix-shaped multipipettor are present.
  • the first bath is filled with the clean rinsing liquid via an inlet.
  • the level of the first tub does not exceed the web height and thereby on the through holes in the second Tray can drain, an overflow or a level sensor is provided.
  • the pipette tips are then immersed in the clean rinsing liquid, filled with this by suction and emptied again through the through-holes, so that the rinsing liquid contaminated with residues of the previously pipetted test substance passes into the second basin. Whether the required relative movement takes place by moving the flushing system or the pipette tips is irrelevant.
  • Such a Spülwannensystem has some significant disadvantages:
  • the object of the invention is to provide a method with which it is possible, using a known Spülwannensystems matrix-like arranged pipette tips or transfer needles to clean completely residue-free effectively and perform the rinsing process time-effective.
  • Essential to the invention is the continuous filling of the lower trough of the flushing trough system with clean rinsing liquid, which in the process is conveyed via the through-holes into the upper trough and discharged therefrom.
  • the pipette tips or transfer needles are introduced into the through-holes of the flushing tub system.
  • the outer surface of the pipette tips or transfer needles is flowed around by the flow generated in the through holes of the clean rinsing liquid and thus cleaned.
  • the thereby dissolving residues of test substance are conveyed by the flow generated by the continuous supply of clean rinsing liquid in the through holes upwards and rinsed in each case over the edge thereof.
  • a decisive advantage of the method according to the invention over a method as it is with a Spülwannensystem according to the DE 196 35 004 C1 is carried out, the safe avoidance of contamination of the container for the clean rinse liquid, which includes in addition to the lower trough in the extended sense, the transformations of the through holes, which protrude into the upper trough as surveys, as well as the contamination of the clean rinsing liquid therein.
  • Safely avoiding the contamination of the through-hole conversions is not only important to prevent the contamination of the clean liquid rising therein, but also to prevent re-introduction of the pipette tips or transfer needles when repeating the flushing process To contaminate touch with the transformations.
  • Another side effect is the possibility of a tighter arrangement of the through holes and thus an increase in the pitch compared to a Spülwannensystem according to the DE 196 35 004 C1 because no vents are required. Center distances of the through holes of less than 9 mm, for example 4.5 mm, 2.25 mm or 1.125 mm are feasible. Also, no overflow or overflow sensor is required, resulting in a constructive simplification. In order to produce a uniform flow of the rinsing liquid in all through holes, the cross section of the lower trough may be tapered to restrict the flow.
  • the edge surface around the through-holes should either be very narrow, or represent a plane surface inclined with respect to the trough bottom of the upper trough.
  • a Spülwannensystem consists essentially of an upper tub 1.1, a lower tub 2.1, a tundish 3 and a plurality of tubes 4, the same dimensions, which are arranged in a matrix, are fixed with one end in the trough 3.
  • the free ends of the tubes 4 protrude through openings 5, which have a larger diameter than the outer diameter of the tubes 4, perpendicular to the tub bottom into the interior of the upper tub 1.1 inside.
  • the attached end of the tube 4 is in each case via a channel 6 with the lower tray 2.1 in combination.
  • the rinsing liquid thereby rises above the channels 6 in the tube 4 upwards and flows over the edge, to then flow through the free area of the openings 5 in the intermediate trough 3. From here, the rinsing liquid can drain through the drain 9 by gravity or be removed by pumping.
  • the inner diameter of the channels 6 are chosen to be much smaller than the inner diameter of the tube. 4 So that the ascending rinsing liquid can leave the tubes 4 unhindered, tubes 4 are used with a small wall thickness. The wall thickness is decisive for the edge surface, which must overflow the rinsing liquid in the horizontal direction. To reduce this edge surface, the tubes 4 may advantageously be chamfered.
  • the cleaning process for the pipette tips or transfer needles usually consists of repeatedly performing the rinsing process.
  • the rinsing process begins by positioning the rinsing system and the matrix-like arrangement of pipette tips or transfer needles relative to one another such that the individual pipette tips or transfer needles are respectively aligned with a small tube 4 above it. By lifting the flushing system or lowering the pipette tips or transfer needles they are immersed in the tube 4. In this case, the outer surface of the pipette tips or transfer needles is flowed around by the flow generated in the tube 4 of the clean rinsing liquid and thus cleaned.
  • the pipette tips In the case of the pipette tips, during the flushing of the outer surface at the same time the pipette interior is filled by suction with clean rinsing liquid. When filled, the pipette tips are raised again or the flushing system is lowered. This is followed by a horizontal relative movement, preferably by half the distance between two adjacent tubes 4 and the dispensing of the pipette contents into the upper tub 1.1.
  • the rinsing process can be repeated as often as required without a complete replacement of the clean rinsing liquid.
  • the effectiveness of the cleaning for the outer surface of the pipette tips or transfer needles depends solely on the duration of the circulation through the clean rinsing liquid and its flow rate. For the effectiveness of cleaning the inner surface of the pipette tips, the number of cycles of suction and ejection, ie the repeated repetition of the flushing process is decisive.
  • a technologically easier to manufacture embodiment of a Spülwannensystems invention is in a second embodiment with reference to Fig. 3 and Fig. 4 described.
  • the second embodiment consists of an upper trough 1.2 and a lower trough 2.2, wherein the bottom of the upper trough 1.2 is formed in the form of juxtaposed webs 7 with through holes 8.
  • the lower trough 2.2 is filled continuously via an inlet 10 with clean rinsing liquid.
  • the rinsing liquid rises over the through holes 8 in the webs 7 after above and flows over the edge into the interior of the upper tub 1.2. From here, the rinsing liquid can be pumped through a drain 9. It is also important here that the through-holes 8 are arranged in the same grid as the pipette tips or transfer needles.
  • the edge region of the ridges 7 should be formed around the through-bores as a plane surface inclined towards the trough bottom of the upper trough 1.2, as in FIG Fig. 4 shown.
  • the upper pan 1.2 is advantageously made of a hydrophobic material or coated with such a material, so that it forms a coherent liquid level and thus a uniform suction of the contaminated flushing liquid is made possible.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Cleaning By Liquid Or Steam (AREA)
  • Cleaning In General (AREA)

Claims (9)

  1. Dispositif de nettoyage pour pointes de pipettes ou aiguilles de transfert, disposées sous la forme d'une matrice, ledit dispositif comprenant un système de bacs de rinçage, comportant un bac inférieur (2) et un bac supérieur (1), et des protubérances entrant dans l'espace intérieur du bac supérieur (1), lesdites protubérances présentant des trous de passage qui sont perpendiculaires au fond du bac supérieur (1) et qui sont disposés de manière à présenter la même trame entre eux que les pointes de pipettes ou aiguilles de transfert, caractérisé en ce que l'on prévoit une pompe, qui est en communication avec le bac inférieur (2) au moyen d'une entrée (10) afin de remplir le bac inférieur (2) en continu avec du liquide de rinçage, et qu'au moins une sortie (9) est prévue sur le bac supérieur (1).
  2. Système de bacs de rinçage selon la revendication 1, caractérisé en ce que lesdites protubérances sont formées par les extrémités de tubes (4) à paroi mince, qui sont fixés par leur autre extrémité dans un bac intermédiaire (3) et font saillie dans le bac supérieur (1) à travers d'ouvertures rondes (5) dans le fond du bac supérieur (1).
  3. Système de bacs de rinçage selon la revendication 2, caractérisé en ce que les ouvertures (5) présentent un diamètre qui est supérieur au diamètre extérieur des tubes (4), de sorte que les ouvertures (5) constituent des sorties (9) et le bac intermédiaire (3) présente une sortie d'extrémité pour l'évacuation du liquide de rinçage par pompe.
  4. Système de bacs de rinçage selon la revendication 2 ou 3, caractérisé en ce que les tubes (4) présentent un chanfrein circonférentiel dans leur région périphérique.
  5. Système de bacs de rinçage selon la revendication 1, caractérisé en ce que lesdites protubérances constituent des entretoises (7) formées sur le fond du bac supérieur (1) et que les trous de passage sont des alésages de passage (8), la région périphérique autour desdites alésages de passage (8) constituant une face plane inclinée vers le fond du bac supérieur (1).
  6. Système de bacs de rinçage selon la revendication 1, caractérisé en ce qu'au moins le bac supérieur (1) est constitué d'un matériau hydrophobique ou est revêtu d'un matériau hydrophobique.
  7. Système de bacs de rinçage selon la revendication 1, caractérisé en ce que la trame, c'est-à-dire la distance de centre à centre des trous de passage, est de moins de 9 mm, en particulier 4,5 mm, 2,25 mm, ou 1,125 mm.
  8. Procédé de nettoyage d'un ensemble matriciel de pointes de pipettes ou aiguilles de transfert, dans lequel procédé ledit ensemble et un système de rinçage d'un dispositif selon la revendication 1 sont positionnés l'un par rapport à l'autre de sorte que chacune des pointes de pipettes ou aiguilles de transfert soit disposée en alignement avec un trou de passage respectif et au-dessus de celui-ci, caractérisé en ce que les pointes de pipettes ou aiguilles de transfert sont immergées dans les trous de passage, le bac inférieur (2) étant en même temps rempli de liquide de rinçage propre, cette dernière montant vers le haut à travers les trous de passage et entourant la surface extérieure des pointes de pipettes ou aiguilles de transfert tout en les nettoyant.
  9. Procédé de nettoyage d'un ensemble matriciel de pointes de pipettes selon la revendication 8, caractérisé en ce que, pendant que le liquide entoure la surface extérieure, l'intérieur des pipettes est rempli par aspiration avec du liquide de rinçage propre, les pointes de pipettes ainsi remplies étant relevées encore une fois ou le système de rinçage étant abaissé, et les pointes de pipettes étant déplacées horizontalement par rapport au système de rinçage afin de décharger les contenus des pipettes dans le bac supérieur (1).
EP00112838A 1999-07-19 2000-06-17 Bac de rinçage Expired - Lifetime EP1070963B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19934090A DE19934090A1 (de) 1999-07-19 1999-07-19 Spülwannensystem
DE19934090 1999-07-19

Publications (3)

Publication Number Publication Date
EP1070963A2 EP1070963A2 (fr) 2001-01-24
EP1070963A3 EP1070963A3 (fr) 2002-09-18
EP1070963B1 true EP1070963B1 (fr) 2008-11-19

Family

ID=7915476

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00112838A Expired - Lifetime EP1070963B1 (fr) 1999-07-19 2000-06-17 Bac de rinçage

Country Status (4)

Country Link
US (1) US6475444B1 (fr)
EP (1) EP1070963B1 (fr)
JP (1) JP2001054772A (fr)
DE (2) DE19934090A1 (fr)

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JP2003066381A (ja) * 2001-05-23 2003-03-05 Novartis Ag 流体で物品を処理するためのシステム及び方法
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JP2008107318A (ja) * 2006-09-27 2008-05-08 Fujifilm Corp 液循環装置、及び、測定装置
US8268238B2 (en) 2006-09-29 2012-09-18 Tyco Healthcare Group Lp System and method for recycling sterilant gas
US7666369B2 (en) 2006-09-29 2010-02-23 Tyco Healthcare Group Lp System and method for recycling sterilant gas
KR101432161B1 (ko) * 2006-11-01 2014-08-20 퀀텀 글로벌 테크놀로지스, 엘엘씨 챔버 부품을 세정하기 위한 방법 및 장치
EP2205365B1 (fr) * 2007-10-17 2017-02-22 Aushon Biosystems Dispositif de lavage de tige à écoulement continu
CN201419170Y (zh) * 2009-03-18 2010-03-10 鸿富锦精密工业（深圳）有限公司 吸嘴清洗装置
DE102012106675B3 (de) * 2012-07-23 2013-10-17 Cybio Ag Absaugvorrichtung für eine matrixförmige Anordnung von Pipettenspitzen
CN102950075B (zh) * 2012-11-19 2015-09-16 苏州农业职业技术学院 喷水量均衡的洗瓶机喷针装置
ES2715391T3 (es) * 2013-05-01 2019-06-04 Douglas Scient Llc Lavado de pipetas
WO2017011243A1 (fr) * 2015-07-10 2017-01-19 Grenova, Llc Dispositif et procédé de lavage de pointe de pipette et système et procédé de distribution de fluide ou de semi-fluide associés
CN109070148B (zh) * 2016-05-11 2022-02-01 西门子医疗保健诊断公司 用于分析仪器的探针清洗站
KR102572150B1 (ko) * 2021-10-19 2023-08-28 송율아 초음파 세척이 가능한 초경봉 지지용 트레이
DE102024101291B4 (de) * 2023-01-20 2025-08-14 Miele & Cie. Kg Reinigungseinrichtung
DE102023123455B3 (de) * 2023-08-31 2025-01-16 Singulus Technologies Aktiengesellschaft Anlagemodul zum Sammeln und Entnehmen von Ausschuss

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Also Published As

Publication number Publication date
EP1070963A3 (fr) 2002-09-18
DE19934090A1 (de) 2001-02-08
JP2001054772A (ja) 2001-02-27
US6475444B1 (en) 2002-11-05
EP1070963A2 (fr) 2001-01-24
DE50015451D1 (de) 2009-01-02

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