EP1092041A4 - Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease - Google Patents

Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease

Info

Publication number
EP1092041A4
EP1092041A4 EP00919763A EP00919763A EP1092041A4 EP 1092041 A4 EP1092041 A4 EP 1092041A4 EP 00919763 A EP00919763 A EP 00919763A EP 00919763 A EP00919763 A EP 00919763A EP 1092041 A4 EP1092041 A4 EP 1092041A4
Authority
EP
European Patent Office
Prior art keywords
exonuclease
nucleic acid
directed evolution
mediated nucleic
acid reassembly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00919763A
Other languages
German (de)
English (en)
Other versions
EP1092041A1 (fr
Inventor
Jay M Short
Tsotne David Djavakhishvili
Gerhard Johann Frey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Diversa Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/276,860 external-priority patent/US6352842B1/en
Priority claimed from US09/332,835 external-priority patent/US6537776B1/en
Application filed by Diversa Corp filed Critical Diversa Corp
Publication of EP1092041A1 publication Critical patent/EP1092041A1/fr
Publication of EP1092041A4 publication Critical patent/EP1092041A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP00919763A 1999-03-26 2000-03-27 Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease Withdrawn EP1092041A4 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US276860 1988-11-28
US09/276,860 US6352842B1 (en) 1995-12-07 1999-03-26 Exonucease-mediated gene assembly in directed evolution
US332835 1999-06-14
US09/332,835 US6537776B1 (en) 1999-06-14 1999-06-14 Synthetic ligation reassembly in directed evolution
PCT/US2000/008245 WO2000058517A1 (fr) 1999-03-26 2000-03-27 Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease

Publications (2)

Publication Number Publication Date
EP1092041A1 EP1092041A1 (fr) 2001-04-18
EP1092041A4 true EP1092041A4 (fr) 2001-09-19

Family

ID=26958167

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00919763A Withdrawn EP1092041A4 (fr) 1999-03-26 2000-03-27 Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease

Country Status (6)

Country Link
EP (1) EP1092041A4 (fr)
JP (1) JP2004500019A (fr)
AU (1) AU4039400A (fr)
CA (1) CA2329122A1 (fr)
MX (1) MXPA00011569A (fr)
WO (1) WO2000058517A1 (fr)

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US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US6500639B2 (en) 1998-10-07 2002-12-31 Maxygen, Inc. DNA shuffling to produce nucleic acids for mycotoxin detoxification
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
WO2000042560A2 (fr) 1999-01-19 2000-07-20 Maxygen, Inc. Methodes de fabrication de chaines de caracteres, de polynucleotides et de polypeptides
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
IL138206A (en) * 1999-02-04 2011-06-30 Verenium Corp Methods for non-stochastic generation of progeny polypeptides and hybrid polynucleotides
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
DE19953854C2 (de) 1999-11-09 2002-01-17 Max Planck Gesellschaft Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
US20010039014A1 (en) 2000-01-11 2001-11-08 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
AU2001268716A1 (en) 2000-06-23 2002-01-08 Maxygen, Inc. Novel chimeric promoters
AU2001272978A1 (en) 2000-06-23 2002-01-08 Maxygen, Inc. Novel co-stimulatory molecules
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
JP4589602B2 (ja) * 2000-12-01 2010-12-01 ヴェレニウム コーポレイション デハロゲナーゼ活性を有する酵素およびその利用方法
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
CA2474172C (fr) 2001-01-25 2012-07-10 Evolva Ltd. Genotheque cellulaire
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
NZ528260A (en) 2001-05-18 2005-09-30 Danisco Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride
EP1840211A1 (fr) 2001-10-31 2007-10-03 Danisco A/S Pyranosone dehydratase de phanerochaete chrysosporium
CA2485506C (fr) 2002-05-17 2012-02-28 Alligator Bioscience Ab Methode de developpement moleculaire in vitro d'une fonction de proteine
MXPA05007654A (es) 2003-01-17 2005-09-30 Danisco Metodo.
EP3323887A3 (fr) 2003-01-17 2018-09-12 DuPont Nutrition Biosciences ApS Procédé de production sur site d'un émulsifiant dans un aliment
KR101169265B1 (ko) 2003-12-24 2012-08-02 대니스코 에이/에스 단백질
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
EA014985B1 (ru) 2004-07-16 2011-04-29 Даниско А/С Способ ферментативного рафинирования пищевых масел гидратацией и применение липидацилтрансферазы для осуществления способа
GB0421598D0 (en) 2004-09-29 2004-10-27 Cambridge Advanced Tech Modification of plant development and morphology
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
ATE464382T1 (de) * 2005-01-10 2010-04-15 Medimmune Ltd Mutageneseverfahren
GB0500417D0 (en) 2005-01-10 2005-02-16 Cambridge Antibody Tech Method of mutagenesis
AU2007344910B2 (en) 2007-01-25 2013-03-28 Dupont Nutrition Biosciences Aps Production of a lipid acyltransferase from transformed Bacillus licheniformis cells
US8143046B2 (en) 2007-02-07 2012-03-27 Danisco Us Inc., Genencor Division Variant Buttiauxella sp. phytases having altered properties
CN103476425B (zh) 2007-03-30 2015-07-15 纽约州州立大学研究基金会 用于疫苗的减毒病毒
NZ585870A (en) 2007-12-21 2012-04-27 Danisco Process for edible oil refining using a lipid acyltransferase
US20100260739A1 (en) 2009-03-09 2010-10-14 Bioatla, Llc Mirac Proteins
GB0908770D0 (en) 2009-04-24 2009-07-01 Danisco Method
WO2010134035A1 (fr) 2009-05-19 2010-11-25 Danisco A/S Utilisation
AR077042A1 (es) 2009-06-12 2011-07-27 Danisco Metodo para tratar una composicion que contiene pirofiofitina
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
WO2011110967A1 (fr) 2010-03-12 2011-09-15 Danisco A/S Procédé
WO2011114251A1 (fr) 2010-03-18 2011-09-22 Danisco A/S Produits alimentaires
BR112012032176A2 (pt) 2010-06-17 2015-09-08 Dupont Nutrition Biosci Aps processo de tratamento de sementes que contém óleo, método de obtenção de óleo de sementes vegetais, processo de produção de um óleo vegetal refinado e óleo vegetal bruto ou refinado que pode ser obtido por meio de um método ou processo
GB201011513D0 (en) 2010-07-08 2010-08-25 Danisco Method
WO2012114232A1 (fr) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Procédé
ES2649912T3 (es) 2011-02-23 2018-01-16 Dupont Nutrition Biosciences Aps Tratamiento enzimático de la clorofila en los aceites vegetales
CA2825628A1 (fr) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Procede
US20140031272A1 (en) 2011-04-08 2014-01-30 Danisco Us Inc. Compositions
WO2013104660A1 (fr) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Procédé de traitement d'une huile végétale comprenant l'hydrolyse de chlorophylle ou d'un dérivé de chlorophylle et une neutralisation caustique partielle
WO2013104659A2 (fr) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Procédé
WO2013160372A1 (fr) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Procédé de traitement d'huile végétale comprenant l'ajout de doses successives de chlorophylle ou d'une enzyme de dégradation de dérivé de chlorophylle
WO2013160374A1 (fr) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Procédé de raffinage d'huile végétale brute comprenant une hydrolyse enzymatique et un recyclage de gommes
EP2909230B1 (fr) 2012-10-19 2019-05-22 Danisco US Inc. Stabilisation de membranes biométriques
CA2934039A1 (fr) 2013-12-19 2015-06-25 Danisco Us Inc. Utilisation d'hydrophobines pour augmenter le transfert de gaz dans des processus de fermentation aerobie
WO2015150372A1 (fr) 2014-04-01 2015-10-08 Dupont Nutrition Biosciences Aps Procédé permettant l'augmentation de rendements de production d'huile de palme brute
AU2015334890B2 (en) 2014-10-24 2021-12-23 Dupont Nutrition Biosciences Aps Proline tolerant tripeptidyl peptidases and uses thereof
US20170306360A1 (en) 2014-10-24 2017-10-26 Danisco Us Inc. Method for producing alcohol by use of a tripeptidyl peptidase
CA3013383A1 (fr) 2016-02-25 2017-08-31 Dupont Nutrition Biosciences Aps Procede de production d'un hydrolysat proteique utilisant la tripeptidyl-peptidase aspergillus
EP3940074A1 (fr) * 2016-07-29 2022-01-19 New England Biolabs, Inc. Procédés et compositions destinés à prévenir la concatémérisation pendant la commutation de matrice

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EP0438292A2 (fr) * 1990-01-17 1991-07-24 NIHON CHEMICAL RESEARCH KABUSHIKI KAISHA also known as JCR PHARMACEUTICALS CO., LTD Procédé pour la préparation d'amorces pour la réaction en chaîne de polymérase à l'aide de ces amorces
WO1991012341A1 (fr) * 1990-02-12 1991-08-22 Fred Hutchinson Cancer Research Center Procede ameliore pour deletions ordonnees a partir d'un point fixe dans un insert clone
WO1997020078A1 (fr) * 1995-11-30 1997-06-05 Maxygen, Inc. Procede d'elaboration de polynucleotides presentant des caracteristiques desirees par selection iterative et recombinaison
WO1997020918A1 (fr) * 1995-12-07 1997-06-12 Recombinant Biocatalysis, Inc. Procede de detection d'une activite enzymatique
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EP0316018A2 (fr) * 1985-03-29 1989-05-17 Cetus Oncology Corporation Modification de séquences d'ADN
EP0438292A2 (fr) * 1990-01-17 1991-07-24 NIHON CHEMICAL RESEARCH KABUSHIKI KAISHA also known as JCR PHARMACEUTICALS CO., LTD Procédé pour la préparation d'amorces pour la réaction en chaîne de polymérase à l'aide de ces amorces
WO1991012341A1 (fr) * 1990-02-12 1991-08-22 Fred Hutchinson Cancer Research Center Procede ameliore pour deletions ordonnees a partir d'un point fixe dans un insert clone
WO1997020078A1 (fr) * 1995-11-30 1997-06-05 Maxygen, Inc. Procede d'elaboration de polynucleotides presentant des caracteristiques desirees par selection iterative et recombinaison
WO1997020918A1 (fr) * 1995-12-07 1997-06-12 Recombinant Biocatalysis, Inc. Procede de detection d'une activite enzymatique
WO1997020950A1 (fr) * 1995-12-08 1997-06-12 Stratagene Mutagenese amelioree, dirigee sur un site d'un adn circulaire
WO1998001581A1 (fr) * 1996-07-09 1998-01-15 Recombinant Biocatalysis, Inc. Methode de remaniement d'adn avec des polynucleotides obtenus par blocage ou interruption d'un processus de synthese ou d'amplification
WO1998038297A1 (fr) * 1997-02-27 1998-09-03 Gesher - Israel Advanced Biotecs (1996) Ltd. Reunion, en une seule operation, de plusieurs fragments d'adn
WO1998058080A1 (fr) * 1997-06-16 1998-12-23 Bioinvent International Ab Procede permettant l'evolution moleculaire in vitro d'une fonction proteique

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See also references of WO0058517A1 *
STEMMER W: "DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 91, no. 22, 25 October 1994 (1994-10-25), pages 10747 - 10751, XP002170160 *
WAYNE M. COCO ET AL.: "DNA shuffling method for generating highly recombined genes and evolved enzymes", NATURE BIOTECHNOLOGY, vol. 19, no. 4, pages 354 - 359 *

Also Published As

Publication number Publication date
EP1092041A1 (fr) 2001-04-18
CA2329122A1 (fr) 2000-10-05
AU4039400A (en) 2000-10-16
MXPA00011569A (es) 2004-12-03
WO2000058517A1 (fr) 2000-10-05
JP2004500019A (ja) 2004-01-08

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