EP1221340A1 - Méthode et dispositif modulaire de détection ou d'analyse quantitative de molécules ou de structures - Google Patents

Méthode et dispositif modulaire de détection ou d'analyse quantitative de molécules ou de structures Download PDF

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Publication number
EP1221340A1
EP1221340A1 EP01100287A EP01100287A EP1221340A1 EP 1221340 A1 EP1221340 A1 EP 1221340A1 EP 01100287 A EP01100287 A EP 01100287A EP 01100287 A EP01100287 A EP 01100287A EP 1221340 A1 EP1221340 A1 EP 1221340A1
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EP
European Patent Office
Prior art keywords
sample
molecules
tube
test
radius
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01100287A
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German (de)
English (en)
Inventor
Heiko Dr. Schwertner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Schwertner Heiko Dr
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to EP01100287A priority Critical patent/EP1221340A1/fr
Publication of EP1221340A1 publication Critical patent/EP1221340A1/fr
Withdrawn legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/028—Modular arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes

Definitions

  • the rapid determination or analysis of molecules or structures is many Areas such as medicine, biotechnology, food industry or forensics an important part of the control, quality assurance or treatment of Human and animal.
  • chromatographic methods e.g. TLC, HPLC, FPLC, GC, CE or column chromatography. These are through immunological (e.g. ELISA, EIA, FIA, RIA etc.) or molecular biological methods (e.g. PCR, primer hybridization) added. A molecule is detected by means of Dye reaction or its physical properties, such as light absorption properties, magnetic or electrochemical properties.
  • OTC tests e.g. pregnancy tests, drug tests
  • strip tests e.g. pH or nickel determination
  • the invention of the universal modular rapid test method on which this patent is based must therefore be adaptable to a variety of different parameters be operated with a large number of sample material and lead to the result without or with little technical equipment. additionally
  • the rapid test procedure should be designed uniformly so that the layperson can use the system only has to learn once and can then use it safely. The expert should be informed about the diverse adaptability of the system and this on can apply a variety of diagnostic parameters so that it can be used in emergencies or difficult situations the system can use or as an upstream test system for screening or screening.
  • test system is intended for a wide variety of areas in which a fast analytics that do not require tools can be used; for example at Food, in human medicine, biotechnology, in forensic analysis in in veterinary medicine or in microbiological processes.
  • the object is achieved by a device which in principle consists of different devices mutually compatible modules solved.
  • the basic module is a tube with an internal flow layer that is also separate can be used and which differ in at least two of each other Zones or areas is divided.
  • the tube is in at least one or more Divide areas that should perform independent tasks.
  • she can consist of different materials, e.g. made of glass, metal, plastic, Ceramics or biopolymers as well as combinations of the materials mentioned. The choice depends on the intended use. By default used a translucent plastic tube.
  • the carrier tube can be designed in various cross-sectional shapes and sizes become. Round, hexagonal and triangular cross-section types have proven particularly useful.
  • the carrier tube material can be made of various materials such as plastic, Glass, quartz, metal or polymers can be produced. Tried and tested have clear and transparent materials here; for example plastics, glass and quartz, since here an evaluation of the test with the help of the human eye is possible. Execution with composite materials is also possible without further ado. Furthermore, an increase in sensitivity by optical detection using fluorine essence, UV or chemical essence can be achieved.
  • a defined volume of fluid can be created as a result of the capillary forces, which depending on the radius and the Test tube material is taken up by simply immersing it in the sample to be tested become. This gives the analyst a defined starting volume that he can refer to in later calculations.
  • the sample ante serves for Recording by simply immersing or inserting it into the sample starting material.
  • the filters serve as a cleaning stage, but can also be coated with chemically active substances or biomolecules as an analytically active zone can be designed, for example, by coupling antibodies to disruptive Substances are removed.
  • the filters can be in cross section, shape, material and Layer can be designed variable.
  • the space between the filters can be filled with different materials, e.g. C18 material, gel or activated carbon. These can be like activated carbon or C18 materials absorb whole groups of substances to undesirable chemical groups to remove from the liquid sample.
  • the test strip base serves as a support for the functional areas and the starting point or derivatization point.
  • the shape is adapted to the test system tube, however planar, curved or kinked.
  • the test strip base can be in different materials, such as plastic, glass, quartz, metal or Polymers. Clear and transparent materials such as Plastic, glass and quartz, since here an evaluation of the test with the help of human Eye is possible. A version with composite materials is also straightforward possible.
  • the functional surfaces are used to hold substances or molecules that are used for Analysis, such as Capture antibodies or small molecules such as Biotin and avidin.
  • the functional surfaces can also be cut out attached to the test carrier base.
  • the functional areas can be in different Materials are made, such as plastic, glass, quartz, metal or polymers. Clear and transparent materials such as plastics, Glass and quartz. A design with composite materials is also straightforward possible.
  • This embodiment variant enables detection by spectroscopic Methods such as UV, IR, laser, visible light, fluorescence or other processes possible.
  • the test strip base is also without the Carrier tube can be used.
  • the test strip base can be used for documentation purposes or serve to further check the result, as well as in further investigations be used.
  • the carrier tube can be designed in various cross-sectional shapes and sizes become. Round, hexagonal and triangular cross-section types have proven particularly useful.
  • the carrier tube material can be made from various materials such as plastic, glass, quartz, metals or polymers. Tried and tested have clear and translucent materials such as plastics, glass and quartz, because here an evaluation of the test with the help of the human eye is possible. Also execution with composite materials is easily possible.
  • the carrier cup is designed so that it is able to test the test system tube in different Hold positions.
  • the closure is usually designed as a twist or clip closure and is Compatible with the closure of the test system tube and / or the encasing tube. This means that sample preparation can be carried out internally and externally.
  • the in Containers inside can be made of fragile material such as glass, Plastic or sponges. Instead of the containers, gels can also be used under pressure, friction, heating, light or electrical Electricity release their ingredients or liquefy. additional fragile containers and / or gels allow the ingredients to be gradually released.
  • the sponge is used to catch excess liquid material while the laser diode, the optical sensor, the conductor tracks etc. form the measuring system.
  • FIG. 008 A compilation of an overall test system using the example of a simple application is shown in FIG. 008. This consists of the basic elements or parts of these described above. A brief description is given in the following table (Tab.002):
  • the so-called start area (Stb) on which the substance to be detected is covalent has been bound, the detection antibody (NAK) is added, so that saturation the substance takes place.
  • particles e.g. gold
  • dyes on the NAK e.g. fluorescein
  • the free substance binds the sample competitively to the detection antibody.
  • the solution, which now contains substance-NAK is generated by means of these capillary forces or Flow forces passed over a capture area (ca).
  • an antibody is used as a positive control given species deviating from the NAK. This migrates with the sample liquid as well as free okadaic acid of the sample on a control layer (KS), on which there is are also covalently bound antibodies that have an affinity for the Fc part own the KAK.
  • KS control layer
  • a multiple parameter system can be developed from this two-parameter system by adding the different NAK to the dye or particle have a further defined binding site for a specific capture antibody (sFAK). These would be bound to separate areas and allow so the determination of several parameters in the sample.
  • sFAK specific capture antibody

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP01100287A 2001-01-04 2001-01-04 Méthode et dispositif modulaire de détection ou d'analyse quantitative de molécules ou de structures Withdrawn EP1221340A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP01100287A EP1221340A1 (fr) 2001-01-04 2001-01-04 Méthode et dispositif modulaire de détection ou d'analyse quantitative de molécules ou de structures

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP01100287A EP1221340A1 (fr) 2001-01-04 2001-01-04 Méthode et dispositif modulaire de détection ou d'analyse quantitative de molécules ou de structures

Publications (1)

Publication Number Publication Date
EP1221340A1 true EP1221340A1 (fr) 2002-07-10

Family

ID=8176148

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01100287A Withdrawn EP1221340A1 (fr) 2001-01-04 2001-01-04 Méthode et dispositif modulaire de détection ou d'analyse quantitative de molécules ou de structures

Country Status (1)

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EP (1) EP1221340A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006002258A1 (de) * 2006-01-17 2007-07-26 Siemens Ag Modul zur Aufbereitung einer biologischen Probe, Biochip-Satz und Verwendung des Moduls
NL1033365C2 (nl) * 2007-02-09 2008-08-12 Medavinci Dev B V Inrichting en werkwijze voor scheiden en analyseren van bloed.
WO2012066032A1 (fr) * 2010-11-16 2012-05-24 D-Tek S.A. Support solide servant à la détection d'analytes
CN109070079A (zh) * 2016-03-30 2018-12-21 索尼公司 样本分离套件、样本分离装置
RU2733517C2 (ru) * 2015-07-17 2020-10-02 Стат-Диагностика Энд Инновэйшн, С.Л. Способ и устройство для разделения и сбора образцов

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4235839A (en) * 1978-12-11 1980-11-25 Olof Vesterberg Device for analysis
US4308028A (en) * 1980-04-14 1981-12-29 Elkins Carlos D Device and method for the chemical testing and microscopic examination of liquid specimens
DE3241922A1 (de) * 1982-11-12 1984-05-17 Walter Sarstedt Kunststoff-Spritzgußwerk, 5223 Nümbrecht Fluiduntersuchungsanordnung
US4876068A (en) * 1989-03-03 1989-10-24 Chemetrics, Inc. Test kit for colorimetric analysis
US5215102A (en) * 1991-10-25 1993-06-01 La Mina Ltd. Capillary blood antigen testing apparatus
EP0737443A2 (fr) * 1989-01-10 1996-10-16 La Mina Ltd. Dispositif pour recueillir un fluide biologique
WO1999021655A1 (fr) * 1997-10-27 1999-05-06 Idexx Laboratories, Inc. Dispositif et procedes permettant de mesurer un analyte dans une solution

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4235839A (en) * 1978-12-11 1980-11-25 Olof Vesterberg Device for analysis
US4308028A (en) * 1980-04-14 1981-12-29 Elkins Carlos D Device and method for the chemical testing and microscopic examination of liquid specimens
DE3241922A1 (de) * 1982-11-12 1984-05-17 Walter Sarstedt Kunststoff-Spritzgußwerk, 5223 Nümbrecht Fluiduntersuchungsanordnung
EP0737443A2 (fr) * 1989-01-10 1996-10-16 La Mina Ltd. Dispositif pour recueillir un fluide biologique
US4876068A (en) * 1989-03-03 1989-10-24 Chemetrics, Inc. Test kit for colorimetric analysis
US5215102A (en) * 1991-10-25 1993-06-01 La Mina Ltd. Capillary blood antigen testing apparatus
WO1999021655A1 (fr) * 1997-10-27 1999-05-06 Idexx Laboratories, Inc. Dispositif et procedes permettant de mesurer un analyte dans une solution

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006002258A1 (de) * 2006-01-17 2007-07-26 Siemens Ag Modul zur Aufbereitung einer biologischen Probe, Biochip-Satz und Verwendung des Moduls
DE102006002258B4 (de) * 2006-01-17 2008-08-21 Siemens Ag Modul zur Aufbereitung einer biologischen Probe, Biochip-Satz und Verwendung des Moduls
US8771609B2 (en) 2006-01-17 2014-07-08 Siemens Aktiengesellschaft Module for processing a biological sample, biochip kit, and use of the module
NL1033365C2 (nl) * 2007-02-09 2008-08-12 Medavinci Dev B V Inrichting en werkwijze voor scheiden en analyseren van bloed.
WO2008097091A1 (fr) * 2007-02-09 2008-08-14 Medavinci Development B.V. Appareil et procédé pour séparer et analyser du sang
US9993816B2 (en) 2007-02-09 2018-06-12 Fabpulous B.V. Apparatus and method for separating and analyzing blood
WO2012066032A1 (fr) * 2010-11-16 2012-05-24 D-Tek S.A. Support solide servant à la détection d'analytes
RU2733517C2 (ru) * 2015-07-17 2020-10-02 Стат-Диагностика Энд Инновэйшн, С.Л. Способ и устройство для разделения и сбора образцов
US10919040B2 (en) 2015-07-17 2021-02-16 Stat-Diagnostica & Innovation, S.L. Method for sample separation and collection
CN109070079A (zh) * 2016-03-30 2018-12-21 索尼公司 样本分离套件、样本分离装置
US11385165B2 (en) 2016-03-30 2022-07-12 Sony Corporation Sample isolation kit, sample isolation device

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