EP1444524A2 - Verfahren zur identifizierung von modulatoren der magnesium chelatase-aktivität und -expression in pflanzen - Google Patents

Verfahren zur identifizierung von modulatoren der magnesium chelatase-aktivität und -expression in pflanzen

Info

Publication number: EP1444524A2
Authority: EP; European Patent Office
Prior art keywords: chelatase; plant; arabidopsis; compound; chl
Prior art date: 2001-09-14
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP01970987A

Other languages

English (en)

French (fr)

Inventor

Jeffrey P. Woessner

Adel Zayed

Jorn Gorlach

Douglas Boyes

Keith Davis

Carol Hamilton

Neil Hoffman

Andreas Kloti

Robert Ascenzi

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Cogenics Icoria Inc

Original Assignee

Paradigm Genetics Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-09-14

Filing date

2001-09-14

Publication date

2004-08-11

2001-09-14 Application filed by Paradigm Genetics Inc filed Critical Paradigm Genetics Inc

2004-08-11 Publication of EP1444524A2 publication Critical patent/EP1444524A2/de

Status Withdrawn legal-status Critical Current

Links

108010086470 magnesium chelatase Proteins 0.000 title claims abstract description 139
238000000034 method Methods 0.000 title claims abstract description 66
230000014509 gene expression Effects 0.000 title claims abstract description 46
230000000694 effects Effects 0.000 title abstract description 26
150000001875 compounds Chemical class 0.000 claims abstract description 59
241000219194 Arabidopsis Species 0.000 claims abstract description 54
239000004009 herbicide Substances 0.000 claims abstract description 39
230000012010 growth Effects 0.000 claims abstract description 29
230000027455 binding Effects 0.000 claims abstract description 17
241000196324 Embryophyta Species 0.000 claims description 91
230000002363 herbicidal effect Effects 0.000 claims description 40
150000001413 amino acids Chemical class 0.000 claims description 15
229920001184 polypeptide Polymers 0.000 claims description 15
108090000765 processed proteins & peptides Proteins 0.000 claims description 15
102000004196 processed proteins & peptides Human genes 0.000 claims description 15
241000219195 Arabidopsis thaliana Species 0.000 claims description 13
239000000126 substance Substances 0.000 claims description 11
230000035899 viability Effects 0.000 claims description 11
230000007423 decrease Effects 0.000 claims description 10
108020004999 messenger RNA Proteins 0.000 claims description 8
238000007421 fluorometric assay Methods 0.000 claims description 2
125000003275 alpha amino acid group Chemical group 0.000 claims 2
230000008635 plant growth Effects 0.000 abstract description 14
238000011161 development Methods 0.000 abstract description 12
230000008121 plant development Effects 0.000 abstract description 7
208000006278 hypochromic anemia Diseases 0.000 abstract description 4
230000008859 change Effects 0.000 abstract description 3
101150118163 h gene Proteins 0.000 abstract description 3
230000005764 inhibitory process Effects 0.000 abstract description 3
238000004383 yellowing Methods 0.000 abstract description 3
230000009547 development abnormality Effects 0.000 abstract description 2
230000009546 growth abnormality Effects 0.000 abstract description 2
210000004027 cell Anatomy 0.000 description 27
108090000623 proteins and genes Proteins 0.000 description 14
238000003556 assay Methods 0.000 description 13
230000000692 anti-sense effect Effects 0.000 description 12
ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 11
ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 11
229960001456 adenosine triphosphate Drugs 0.000 description 11
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 9
102000004190 Enzymes Human genes 0.000 description 9
108090000790 Enzymes Proteins 0.000 description 9
239000003446 ligand Substances 0.000 description 9
JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 8
230000003993 interaction Effects 0.000 description 8
229910001425 magnesium ion Inorganic materials 0.000 description 8
102000004169 proteins and genes Human genes 0.000 description 8
239000000243 solution Substances 0.000 description 8
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
238000003780 insertion Methods 0.000 description 6
230000037431 insertion Effects 0.000 description 6
241000589158 Agrobacterium Species 0.000 description 5
108020004414 DNA Proteins 0.000 description 5
235000002637 Nicotiana tabacum Nutrition 0.000 description 5
244000061176 Nicotiana tabacum Species 0.000 description 5
238000001514 detection method Methods 0.000 description 5
230000018109 developmental process Effects 0.000 description 5
238000000338 in vitro Methods 0.000 description 5
239000003112 inhibitor Substances 0.000 description 5
108091033319 polynucleotide Proteins 0.000 description 5
102000040430 polynucleotide Human genes 0.000 description 5
239000002157 polynucleotide Substances 0.000 description 5
210000001519 tissue Anatomy 0.000 description 5
230000009261 transgenic effect Effects 0.000 description 5
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
229920000936 Agarose Polymers 0.000 description 4
QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
108020005544 Antisense RNA Proteins 0.000 description 4
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
229930002875 chlorophyll Natural products 0.000 description 4
235000019804 chlorophyll Nutrition 0.000 description 4
ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 4
239000003184 complementary RNA Substances 0.000 description 4
230000003247 decreasing effect Effects 0.000 description 4
230000005284 excitation Effects 0.000 description 4
239000012634 fragment Substances 0.000 description 4
239000000203 mixture Substances 0.000 description 4
UHSGPDMIQQYNAX-UHFFFAOYSA-N protoporphyrinogen Chemical compound C1C(=C(C=2C=C)C)NC=2CC(=C(C=2CCC(O)=O)C)NC=2CC(N2)=C(CCC(O)=O)C(C)=C2CC2=C(C)C(C=C)=C1N2 UHSGPDMIQQYNAX-UHFFFAOYSA-N 0.000 description 4
239000000758 substrate Substances 0.000 description 4
241000588724 Escherichia coli Species 0.000 description 3
102100039556 Galectin-4 Human genes 0.000 description 3
101000608765 Homo sapiens Galectin-4 Proteins 0.000 description 3
240000005979 Hordeum vulgare Species 0.000 description 3
235000007340 Hordeum vulgare Nutrition 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
108091023040 Transcription factor Proteins 0.000 description 3
102000040945 Transcription factor Human genes 0.000 description 3
230000002159 abnormal effect Effects 0.000 description 3
125000000539 amino acid group Chemical group 0.000 description 3
238000006243 chemical reaction Methods 0.000 description 3
238000010276 construction Methods 0.000 description 3
238000007824 enzymatic assay Methods 0.000 description 3
238000002060 fluorescence correlation spectroscopy Methods 0.000 description 3
230000035772 mutation Effects 0.000 description 3
VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
210000000056 organ Anatomy 0.000 description 3
230000000243 photosynthetic effect Effects 0.000 description 3
229950003776 protoporphyrin Drugs 0.000 description 3
SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 3
230000009466 transformation Effects 0.000 description 3
108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 2
ZBMRKNMTMPPMMK-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid;azane Chemical compound [NH4+].CP(O)(=O)CCC(N)C([O-])=O ZBMRKNMTMPPMMK-UHFFFAOYSA-N 0.000 description 2
241000894006 Bacteria Species 0.000 description 2
108010003118 Bacteriochlorophylls Proteins 0.000 description 2
235000010662 Bidens pilosa Nutrition 0.000 description 2
244000104272 Bidens pilosa Species 0.000 description 2
KSFOVUSSGSKXFI-GAQDCDSVSA-N CC1=C/2NC(\C=C3/N=C(/C=C4\N\C(=C/C5=N/C(=C\2)/C(C=C)=C5C)C(C=C)=C4C)C(C)=C3CCC(O)=O)=C1CCC(O)=O Chemical compound CC1=C/2NC(\C=C3/N=C(/C=C4\N\C(=C/C5=N/C(=C\2)/C(C=C)=C5C)C(C=C)=C4C)C(C)=C3CCC(O)=O)=C1CCC(O)=O KSFOVUSSGSKXFI-GAQDCDSVSA-N 0.000 description 2
240000006122 Chenopodium album Species 0.000 description 2
244000152970 Digitaria sanguinalis Species 0.000 description 2
235000010823 Digitaria sanguinalis Nutrition 0.000 description 2
244000058871 Echinochloa crus-galli Species 0.000 description 2
230000005526 G1 to G0 transition Effects 0.000 description 2
229920002148 Gellan gum Polymers 0.000 description 2
102000053187 Glucuronidase Human genes 0.000 description 2
108010060309 Glucuronidase Proteins 0.000 description 2
239000005561 Glufosinate Substances 0.000 description 2
240000004296 Lolium perenne Species 0.000 description 2
SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 description 2
108091028043 Nucleic acid sequence Proteins 0.000 description 2
240000006928 Persicaria lapathifolia Species 0.000 description 2
108700008625 Reporter Genes Proteins 0.000 description 2
240000004808 Saccharomyces cerevisiae Species 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
229930006000 Sucrose Natural products 0.000 description 2
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
229910021529 ammonia Inorganic materials 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
DSJXIQQMORJERS-AGGZHOMASA-M bacteriochlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC([C@H](CC)[C@H]3C)=[N+]4C3=CC3=C(C(C)=O)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 DSJXIQQMORJERS-AGGZHOMASA-M 0.000 description 2
230000015572 biosynthetic process Effects 0.000 description 2
239000000872 buffer Substances 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
230000001419 dependent effect Effects 0.000 description 2
238000004520 electroporation Methods 0.000 description 2
238000000295 emission spectrum Methods 0.000 description 2
230000002255 enzymatic effect Effects 0.000 description 2
238000005558 fluorometry Methods 0.000 description 2
WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
230000001771 impaired effect Effects 0.000 description 2
229930027917 kanamycin Natural products 0.000 description 2
229960000318 kanamycin Drugs 0.000 description 2
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
229930182823 kanamycin A Natural products 0.000 description 2
231100000518 lethal Toxicity 0.000 description 2
230000001665 lethal effect Effects 0.000 description 2
229910001629 magnesium chloride Inorganic materials 0.000 description 2
238000004949 mass spectrometry Methods 0.000 description 2
244000005700 microbiome Species 0.000 description 2
238000012986 modification Methods 0.000 description 2
230000004048 modification Effects 0.000 description 2
239000012071 phase Substances 0.000 description 2
229920003023 plastic Polymers 0.000 description 2
238000007747 plating Methods 0.000 description 2
239000000047 product Substances 0.000 description 2
238000000159 protein binding assay Methods 0.000 description 2
238000005204 segregation Methods 0.000 description 2
239000002689 soil Substances 0.000 description 2
238000009331 sowing Methods 0.000 description 2
241000894007 species Species 0.000 description 2
238000005507 spraying Methods 0.000 description 2
239000005720 sucrose Substances 0.000 description 2
238000013518 transcription Methods 0.000 description 2
230000035897 transcription Effects 0.000 description 2
241000219144 Abutilon Species 0.000 description 1
240000000321 Abutilon grandifolium Species 0.000 description 1
241000589155 Agrobacterium tumefaciens Species 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
235000013479 Amaranthus retroflexus Nutrition 0.000 description 1
244000237956 Amaranthus retroflexus Species 0.000 description 1
241000192542 Anabaena Species 0.000 description 1
241001520750 Arabidopsis arenosa Species 0.000 description 1
241000395972 Arabidopsis cebennensis Species 0.000 description 1
241000396001 Arabidopsis croatica Species 0.000 description 1
241001610441 Arabidopsis halleri Species 0.000 description 1
241000610258 Arabidopsis lyrata Species 0.000 description 1
241000190586 Arabidopsis neglecta Species 0.000 description 1
241000190571 Arabidopsis suecica Species 0.000 description 1
108010016529 Bacillus amyloliquefaciens ribonuclease Proteins 0.000 description 1
244000062995 Cassia occidentalis Species 0.000 description 1
235000001948 Cassia occidentalis Nutrition 0.000 description 1
235000009344 Chenopodium album Nutrition 0.000 description 1
235000011498 Chenopodium album var missouriense Nutrition 0.000 description 1
235000013328 Chenopodium album var. album Nutrition 0.000 description 1
235000014052 Chenopodium album var. microphyllum Nutrition 0.000 description 1
235000014050 Chenopodium album var. stevensii Nutrition 0.000 description 1
235000013012 Chenopodium album var. striatum Nutrition 0.000 description 1
108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
KZBUYRJDOAKODT-UHFFFAOYSA-N Chlorine Chemical compound ClCl KZBUYRJDOAKODT-UHFFFAOYSA-N 0.000 description 1
108020004635 Complementary DNA Proteins 0.000 description 1
108091035707 Consensus sequence Proteins 0.000 description 1
102000004420 Creatine Kinase Human genes 0.000 description 1
108010042126 Creatine kinase Proteins 0.000 description 1
241000607074 Crucihimalaya himalaica Species 0.000 description 1
241001310865 Crucihimalaya wallichii Species 0.000 description 1
241000206573 Cyanophora paradoxa Species 0.000 description 1
239000003154 D dimer Substances 0.000 description 1
230000004568 DNA-binding Effects 0.000 description 1
235000001602 Digitaria X umfolozi Nutrition 0.000 description 1
235000017898 Digitaria ciliaris Nutrition 0.000 description 1
235000005476 Digitaria cruciata Nutrition 0.000 description 1
235000006830 Digitaria didactyla Nutrition 0.000 description 1
235000005804 Digitaria eriantha ssp. eriantha Nutrition 0.000 description 1
LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 1
235000014716 Eleusine indica Nutrition 0.000 description 1
108700039887 Essential Genes Proteins 0.000 description 1
241000195619 Euglena gracilis Species 0.000 description 1
244000048459 Euphorbia cyathophora Species 0.000 description 1
235000010469 Glycine max Nutrition 0.000 description 1
244000068988 Glycine max Species 0.000 description 1
108010068250 Herpes Simplex Virus Protein Vmw65 Proteins 0.000 description 1
101000582320 Homo sapiens Neurogenic differentiation factor 6 Proteins 0.000 description 1
206010020649 Hyperkeratosis Diseases 0.000 description 1
235000021506 Ipomoea Nutrition 0.000 description 1
241000207783 Ipomoea Species 0.000 description 1
241000207890 Ipomoea purpurea Species 0.000 description 1
241000209510 Liliopsida Species 0.000 description 1
235000003403 Limnocharis flava Nutrition 0.000 description 1
108060001084 Luciferase Proteins 0.000 description 1
239000005089 Luciferase Substances 0.000 description 1
239000006137 Luria-Bertani broth Substances 0.000 description 1
108010059343 MM Form Creatine Kinase Proteins 0.000 description 1
235000012629 Mentha aquatica Nutrition 0.000 description 1
241000203407 Methanocaldococcus jannaschii Species 0.000 description 1
102100030589 Neurogenic differentiation factor 6 Human genes 0.000 description 1
241000326556 Odontella <springtail> Species 0.000 description 1
241001310889 Olimarabidopsis cabulica Species 0.000 description 1
241001279369 Olimarabidopsis pumila Species 0.000 description 1
241001644833 Olisthodiscus luteus Species 0.000 description 1
108700026244 Open Reading Frames Proteins 0.000 description 1
238000010222 PCR analysis Methods 0.000 description 1
235000011999 Panicum crusgalli Nutrition 0.000 description 1
239000001888 Peptone Substances 0.000 description 1
108010080698 Peptones Proteins 0.000 description 1
235000017337 Persicaria hydropiper Nutrition 0.000 description 1
235000004442 Polygonum persicaria Nutrition 0.000 description 1
241000206609 Porphyra Species 0.000 description 1
241000191023 Rhodobacter capsulatus Species 0.000 description 1
235000008406 SarachaNachtschatten Nutrition 0.000 description 1
238000012300 Sequence Analysis Methods 0.000 description 1
235000005775 Setaria Nutrition 0.000 description 1
241000232088 Setaria <nematode> Species 0.000 description 1
235000002248 Setaria viridis Nutrition 0.000 description 1
240000003461 Setaria viridis Species 0.000 description 1
235000010086 Setaria viridis var. viridis Nutrition 0.000 description 1
240000006410 Sida spinosa Species 0.000 description 1
241000700584 Simplexvirus Species 0.000 description 1
239000005708 Sodium hypochlorite Substances 0.000 description 1
241000207763 Solanum Species 0.000 description 1
235000004790 Solanum aculeatissimum Nutrition 0.000 description 1
235000008424 Solanum demissum Nutrition 0.000 description 1
235000018253 Solanum ferox Nutrition 0.000 description 1
235000000208 Solanum incanum Nutrition 0.000 description 1
235000013131 Solanum macrocarpon Nutrition 0.000 description 1
235000002594 Solanum nigrum Nutrition 0.000 description 1
244000061457 Solanum nigrum Species 0.000 description 1
235000009869 Solanum phureja Nutrition 0.000 description 1
235000000341 Solanum ptychanthum Nutrition 0.000 description 1
235000017622 Solanum xanthocarpum Nutrition 0.000 description 1
241000192581 Synechocystis sp. Species 0.000 description 1
UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 description 1
108700026226 TATA Box Proteins 0.000 description 1
241000395969 Transberingia bursifolia Species 0.000 description 1
239000007997 Tricine buffer Substances 0.000 description 1
239000007983 Tris buffer Substances 0.000 description 1
ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical class O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 1
244000067505 Xanthium strumarium Species 0.000 description 1
239000002253 acid Substances 0.000 description 1
230000004913 activation Effects 0.000 description 1
239000012190 activator Substances 0.000 description 1
230000004075 alteration Effects 0.000 description 1
230000003321 amplification Effects 0.000 description 1
239000008346 aqueous phase Substances 0.000 description 1
238000002869 basic local alignment search tool Methods 0.000 description 1
102000005936 beta-Galactosidase Human genes 0.000 description 1
108010005774 beta-Galactosidase Proteins 0.000 description 1
239000007844 bleaching agent Substances 0.000 description 1
210000004899 c-terminal region Anatomy 0.000 description 1
229940041514 candida albicans extract Drugs 0.000 description 1
238000006555 catalytic reaction Methods 0.000 description 1
238000000423 cell based assay Methods 0.000 description 1
238000004113 cell culture Methods 0.000 description 1
230000003833 cell viability Effects 0.000 description 1
238000005919 chelatase reaction Methods 0.000 description 1
230000009920 chelation Effects 0.000 description 1
230000002759 chromosomal effect Effects 0.000 description 1
239000002299 complementary DNA Substances 0.000 description 1
238000011109 contamination Methods 0.000 description 1
230000007812 deficiency Effects 0.000 description 1
KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
MWYMHZINPCTWSB-UHFFFAOYSA-N dimethylsilyloxy-dimethyl-trimethylsilyloxysilane Chemical class C[SiH](C)O[Si](C)(C)O[Si](C)(C)C MWYMHZINPCTWSB-UHFFFAOYSA-N 0.000 description 1
230000008034 disappearance Effects 0.000 description 1
210000002257 embryonic structure Anatomy 0.000 description 1
230000007613 environmental effect Effects 0.000 description 1
238000001952 enzyme assay Methods 0.000 description 1
241001233957 eudicotyledons Species 0.000 description 1
210000003527 eukaryotic cell Anatomy 0.000 description 1
239000000284 extract Substances 0.000 description 1
108091006047 fluorescent proteins Proteins 0.000 description 1
102000034287 fluorescent proteins Human genes 0.000 description 1
230000004927 fusion Effects 0.000 description 1
239000011521 glass Substances 0.000 description 1
150000003278 haem Chemical class 0.000 description 1
238000004128 high performance liquid chromatography Methods 0.000 description 1
238000009396 hybridization Methods 0.000 description 1
239000001257 hydrogen Substances 0.000 description 1
229910052739 hydrogen Inorganic materials 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
238000007901 in situ hybridization Methods 0.000 description 1
238000000099 in vitro assay Methods 0.000 description 1
238000011534 incubation Methods 0.000 description 1
230000008595 infiltration Effects 0.000 description 1
238000001764 infiltration Methods 0.000 description 1
230000002401 inhibitory effect Effects 0.000 description 1
238000000670 ligand binding assay Methods 0.000 description 1
239000011777 magnesium Substances 0.000 description 1
238000005259 measurement Methods 0.000 description 1
239000002609 medium Substances 0.000 description 1
230000000442 meristematic effect Effects 0.000 description 1
MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
238000002493 microarray Methods 0.000 description 1
238000012544 monitoring process Methods 0.000 description 1
239000000178 monomer Substances 0.000 description 1
239000006870 ms-medium Substances 0.000 description 1
230000009871 nonspecific binding Effects 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
239000002245 particle Substances 0.000 description 1
235000019319 peptone Nutrition 0.000 description 1
210000000745 plant chromosome Anatomy 0.000 description 1
229920000233 poly(alkylene oxides) Polymers 0.000 description 1
238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
238000004382 potting Methods 0.000 description 1
239000002243 precursor Substances 0.000 description 1
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
238000000746 purification Methods 0.000 description 1
230000002285 radioactive effect Effects 0.000 description 1
239000011535 reaction buffer Substances 0.000 description 1
238000003753 real-time PCR Methods 0.000 description 1
230000009467 reduction Effects 0.000 description 1
230000003362 replicative effect Effects 0.000 description 1
239000004576 sand Substances 0.000 description 1
238000007789 sealing Methods 0.000 description 1
UMCYUPVKJYJUCD-UHFFFAOYSA-M sodium 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid hydroxide Chemical compound [OH-].[Na+].OCC(CO)(CO)NCC(O)=O UMCYUPVKJYJUCD-UHFFFAOYSA-M 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
230000009870 specific binding Effects 0.000 description 1
238000002798 spectrophotometry method Methods 0.000 description 1
230000001954 sterilising effect Effects 0.000 description 1
238000004659 sterilization and disinfection Methods 0.000 description 1
238000006467 substitution reaction Methods 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
238000004809 thin layer chromatography Methods 0.000 description 1
RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical class CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 1
108091006106 transcriptional activators Proteins 0.000 description 1
230000026683 transduction Effects 0.000 description 1
238000010361 transduction Methods 0.000 description 1
238000001890 transfection Methods 0.000 description 1
238000013519 translation Methods 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
241000701447 unidentified baculovirus Species 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1
238000001262 western blot Methods 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1

Classifications

- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value

Definitions

the invention relates generally to plant molecular biology.
the invention relates to methods for the identification of compounds that regulate plant growth and development through the modulation of magnesium chelatase expression or activity.
binding refers to an interaction between Mg-chelatase or a subunit thereof, and a molecule or compound, wherein the interaction is dependent upon the primary amino acid sequence or the conformation of Mg-chelatase and/or the Mg-chelatase subunit.
the Mg-chelatases useful in the methods of the invention can be from any species of Arabidopsis.
Arabidopsis species include, but are not limited to, Arabidopsis arenosa, Arabidopsis bursifolia, Arabidopsis cebennensis, Arabidopsis croatica, Arabidopsis grtffithiana, Arabidopsis halleri, Arabidopsis himalaica, Arabidopsis korshinskyi, Arabidopsis lyrata, Arabidopsis neglecta, Arabidopsis pumila, Arabidopsis suecica, Arabidopsis thaliana and Arabidopsis wallichii.
Mg-chelatase subunits are added to 6 ⁇ M deuteropo ⁇ hyrin IX, 5 mM ATP, 25 mM creatine kinase, 25 MgCl , 2.5 units creatine kinase, 20 mM Tris pH 9.0 in a volume of 50 ⁇ l, and incubated for 20 minutes at 30°C.
the reaction is stopped by the addition of 1 ml acetone/water/25% ammonia (80:20: 1 , vol/vol/vol) and 200 ⁇ l hexane.
the phases are then separated by brief centrifugation, and the emission spectrum of the bottom acetone phase is determined form 550 to 650 with an excitation wavelength of 408 nm.
the driver expression cassette was introduced into Arabidopsis thaliana by agroinfection. Transgenic plants that stably expressed the driver transcription factor were obtained.

Landscapes

Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Zoology (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
General Health & Medical Sciences (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Environmental Sciences (AREA)
Biophysics (AREA)
Molecular Biology (AREA)
Immunology (AREA)
Biotechnology (AREA)
Analytical Chemistry (AREA)
Physics & Mathematics (AREA)
Agronomy & Crop Science (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
Pest Control & Pesticides (AREA)
Plant Pathology (AREA)
Dentistry (AREA)
Biochemistry (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Agricultural Chemicals And Associated Chemicals (AREA)
Enzymes And Modification Thereof (AREA)

EP01970987A 2001-09-14 2001-09-14 Verfahren zur identifizierung von modulatoren der magnesium chelatase-aktivität und -expression in pflanzen Withdrawn EP1444524A2 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
PCT/US2001/028761 WO2002022857A2 (en)	2000-09-12	2001-09-14	Methods for the identification of modulators of magnesium chelatase expression or activity in plants

Publications (1)

Publication Number	Publication Date
EP1444524A2 true EP1444524A2 (de)	2004-08-11

Family

ID=32653968

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP01970987A Withdrawn EP1444524A2 (de)	2001-09-14	2001-09-14	Verfahren zur identifizierung von modulatoren der magnesium chelatase-aktivität und -expression in pflanzen

Country Status (2)

Country	Link
EP (1)	EP1444524A2 (de)
WO (1)	WO2002022857A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US7329489B2 (en)	2000-04-14	2008-02-12	Matabolon, Inc.	Methods for drug discovery, disease treatment, and diagnosis using metabolomics
US20020009740A1 (en)	2000-04-14	2002-01-24	Rima Kaddurah-Daouk	Methods for drug discovery, disease treatment, and diagnosis using metabolomics
WO2008033575A2 (en)	2006-09-15	2008-03-20	Metabolon, Inc.	Methods of identifying biochemical pathways

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE19717656A1 (de) *	1997-04-25	1998-10-29	Hoechst Schering Agrevo Gmbh	DNA-Sequenzen kodierend für die Untereinheit CHLD pflanzlicher Magnesium-Chelatasen, sowie Verfahren zur Aktivitätsbestimmung pflanzlicher Magnesium-Chelatasen
US6570063B1 (en) *	1999-06-04	2003-05-27	E.I. Dupont De Nemours And Company	Magnesium chelatase

2001
- 2001-09-14 EP EP01970987A patent/EP1444524A2/de not_active Withdrawn
- 2001-09-14 WO PCT/US2001/028761 patent/WO2002022857A2/en not_active Ceased

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0222857A3 *

Also Published As

Publication number	Publication date
WO2002022857A2 (en)	2002-03-21
WO2002022857A3 (en)	2003-06-19
WO2002022857A8 (en)	2004-06-17

Legal Events

Date	Code	Title	Description
2004-06-25	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2004-08-11	17P	Request for examination filed	Effective date: 20040319
2004-08-11	AK	Designated contracting states	Kind code of ref document: A2 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
2004-08-11	AX	Request for extension of the european patent	Extension state: AL LT LV MK RO SI
2004-09-08	RIC1	Information provided on ipc code assigned before grant	Ipc: 7C 12Q 1/25 B Ipc: 7C 12Q 1/00 A
2004-10-20	RIN1	Information on inventor provided before grant (corrected)	Inventor name: ASCENZI, ROBERT Inventor name: KLOTI, ANDREAS Inventor name: HOFFMAN, NEIL Inventor name: HAMILTON, CAROL Inventor name: DAVIS, KEITH Inventor name: BOYES, DOUGLAS Inventor name: GORLACH, JORN Inventor name: ZAYED, ADEL Inventor name: WOESSNER, JEFFREY, P.
2005-03-02	RAP1	Party data changed (applicant data changed or rights of an application transferred)	Owner name: ICORIA, INC.
2006-08-25	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN
2006-09-27	18D	Application deemed to be withdrawn	Effective date: 20060401

Publication	Publication Date	Title
Chapagain et al.	2018	Oryza sativa salt-induced RING E3 ligase 2 (OsSIRP2) acts as a positive regulator of transketolase in plant response to salinity and osmotic stress
US20060160700A1 (en)	2006-07-20	Nadh-dependent cytochrome b5 reductase as target for herbicides
EP1444524A2 (de)	2004-08-11	Verfahren zur identifizierung von modulatoren der magnesium chelatase-aktivität und -expression in pflanzen
US6582900B1 (en)	2003-06-24	Methods for the identification of modulators of magnesium chelatase expression or activity in plants
US20030108977A1 (en)	2003-06-12	Methods for the identification of inhibitors of pyruvate orthophosphate dikinase expression or activity in plants
US20050112715A1 (en)	2005-05-26	Methods for the identification of inhibitors of pectin esterase expression or activity in plants
WO2002046451A2 (en)	2002-06-13	Methods for the identification of inhibitors of cysteine syhthase in plants
US20040229208A1 (en)	2004-11-18	Methods for the identification of inhibitors of CAX1-like Ca+2/H+ antiporter activity in plants
US20050101485A1 (en)	2005-05-12	Methods for the identification of inhibitors of biotin synthase expression or activity in plants
US6770452B1 (en)	2004-08-03	Methods for the identification of inhibitors of serine acetyltransferase activity in plants
EP1386008A1 (de)	2004-02-04	Verfahren zur identifizierung von inhibitoren der expression oder aktivität von biotimsynthase in pflanzen
WO2002052035A2 (en)	2002-07-04	Methods for identification of inhibitors of cinnamyl alcohol dehydrogenase in plants
US20030186278A1 (en)	2003-10-02	Methods for the identification of inhibitors of 1-aminocyclopropane-1-carboxylate oxidase expression or activity in plants
US20020177527A1 (en)	2002-11-28	Methods for the identification of inhibitors of 2'-hydroxyisoflavone reductase expression or activity in plants
US20040265789A1 (en)	2004-12-30	Methods for the identification of inhibitors of 3-oxo-5-alpha-steroid 4-dehydrogenase expression or activity in plants
US20040248152A1 (en)	2004-12-09	Methods for the identification of inhibitors of carbonic anhydrase expression or activity in plants
US20050100879A1 (en)	2005-05-12	Methods for the identification of inhibitors of flavanone 3-hydroxylase expression or activity in plants
WO2002071841A2 (en)	2002-09-19	Methods for the identification of inhibitors of argininosuccinate synthase expression or activity in plants
US20040248228A1 (en)	2004-12-09	Methods for the identification of inhibitors of ferredoxin NADP oxidoreductase expression or activity in plants
WO2003040334A2 (en)	2003-05-15	Methods for the identification of inhibitors of 4-coumarate-coa ligase expression or activity in plants
US20040191851A1 (en)	2004-09-30	Methods for the identification of inhibitors of lipid transfer protein activity in plants
US20040191852A1 (en)	2004-09-30	Methods for the identification of inhibitors of NADPH:protochlorophyllide oxidoreductase activity in plants
US20030113786A1 (en)	2003-06-19	Methods for the identification of inhibitors of thioredoxin expression or activity in plants
EP1390484A2 (de)	2004-02-25	Verfahren zur identifizierung von inhibitoren der expression oder aktivität von pektinesterase in pflanzen
US20040265836A1 (en)	2004-12-30	Methods for the identification of inhibitors of C4 Long Chain Base Hydroxylase activity in plants