EP1664293A2 - Activateurs plasminogenes a capacite de liaison lysine reduite - Google Patents

Activateurs plasminogenes a capacite de liaison lysine reduite

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Publication number
EP1664293A2
EP1664293A2 EP04765139A EP04765139A EP1664293A2 EP 1664293 A2 EP1664293 A2 EP 1664293A2 EP 04765139 A EP04765139 A EP 04765139A EP 04765139 A EP04765139 A EP 04765139A EP 1664293 A2 EP1664293 A2 EP 1664293A2
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EP
European Patent Office
Prior art keywords
plasminogen
plasminogen activator
use according
dspa
activator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP04765139A
Other languages
German (de)
English (en)
Inventor
Wolfgang Söhngen
Oliver Kops
Vincent School of Biological Sciences ELLIS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Paion Deutschland GmbH
Original Assignee
Paion Deutschland GmbH
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Filing date
Publication date
Application filed by Paion Deutschland GmbH filed Critical Paion Deutschland GmbH
Priority to EP10011604A priority Critical patent/EP2368984A1/fr
Publication of EP1664293A2 publication Critical patent/EP1664293A2/fr
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/49—Urokinase; Tissue plasminogen activator
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Definitions

  • the invention relates to an advantageous use of plasminogen activators and claims the priority of German patent application 103 42 518.7, to which reference is made in terms of content.
  • thrombolysis as a therapy option for various thromboembolic diseases is considered largely completed.
  • the main interest of thrombolysis research is therefore the further development and modification of known thrombolytics and / or the improvement of accompanying therapies.
  • tissue plasminogen activator t-PA
  • fibrin selectivity an important thrombolytic agent as the starting point for new thrombolytic agents
  • urokinase an important thrombolytic agent as the starting point for new thrombolytic agents
  • tissue plasminogen activator t-PA
  • t-PA tissue plasminogen activator
  • Further developments of this plasminogen activator are the deletion mutants reteplase and lanoteplase as well as tenecteplase.
  • the tissue plasminogen activator is a single-chain glycoprotein consisting of 527 amino acids.
  • the initially single-chain molecule (single-chain; sct-PA) is split into a two-chain form (two chain; tct-PA) by proteolysis.
  • the t-PA has certain structural and functional domains.
  • the N-terminal partial chain comprises the so-called finger domain (F, Ser1 - Lys49), the epidermal growth factor domain (E, Ser ⁇ O - Asp87) and the two kinkle domains (K1, Thr88 - Gly176; and K2, Asn177 - Cys261).
  • the C-terminal chain with a serine proteinase domain (P) comprises the amino acids Ser262 to Pro527.
  • the C-terminal chain forms the active center with the amino acids His322, Asp371 and Ser478.
  • t-PA and its recombinant variants and their production are the subject of, for example, US Pat. No. 4,766,075 and various publications (such as Bode and Renatus: Tissue-type plasminogen activator: variants and crystal / soiution structures demarcate structural determinants of function, Current Opinion in Structural Biology 1997, 7: 865-872).
  • a schematic representation of the t-PA structure is attached as Fig. 1.
  • the sct-PA is converted into the tct-PA form by cleavage.
  • the cleavage takes place at the bond between Arg275 and IIe276.
  • the two chains are then held together only via a single disulfide bridge between Serin264 and Serin395.
  • This numbering of the t-PA cleavage site corresponds to that chosen by Bode and Renatus (op. Cit.). However, other authors use a different numbering and define the cleavage or activation site as R15-I16 or R310-I311. However, the positions defined in this way do not differ functionally and structurally.
  • tissue plasminogen activator (t-PA; old plase) can activate plasminogen to plasmin.
  • Kinetic constants show that t-PA only activates circulating plasminogen with weak activity.
  • the plasminogen bound to fibrin is activated with a catalytic potency improved by three powers of ten compared to the free plasminogen. Accordingly, the thrombolytic property of the tissue plasminogen activator is greatly enhanced by the presence of fibrin.
  • a relative fibrin selectivity with a factor of 500 to 1000 is given for t-PA.
  • the catalytic effect can also be increased by the Interaction of the piasminogen activator with ⁇ -amyloid or fibrinogen can be achieved.
  • the activation of t-PA by fibrinogen is comparable to the activation by ß-amyloid.
  • the action of the piasminogen activator is controlled physiologically by inhibitors, with the plasminogen activator inhibitor I (PAI-1) being the main antagonist.
  • PAI-1 the plasminogen activator inhibitor I
  • PAI-1 the plasminogen activator inhibitor I
  • the structure of the catalytic center is changed so that an activation reaction with the plasminogen can no longer take place (Bennet WF, Paoni NF, Keyt BA, Botstein D, Jones AJS, Presta L, Wurm FM, Zoller M: High resolution analysis of functional determinants in human tissue-type plasminogen activator. Journal of Biological Chemistry 1991; 266: 5191-5201).
  • tissue plasminogen activator is rapidly metabolized by the liver. Therefore, a patient's hepatic insufficiency prolongs the plasma half-life (Emeis JJ, van den Hoogen CM, Jense D: Hepatic clearance of tissue-type plasminogen activator in rats, Thomb Haemostas 1985; 54: 661-664; Tiefenbrunn AJ, Robison AK , Kurnik PB, Ludbrook PA, Sobel BE. Clinical pharmacology in patients with evolving myocardial infection of tissue plasminogen activator produced by recombinant DNA technology Circulation 1985; 71: 110-116).
  • T-PA is the only thrombolytic currently approved for stroke treatment by the Food and Drug Administration (FDA) in the United States.
  • FDA Food and Drug Administration
  • t-PA shows the expected positive thrombolytic effects on the one hand in the treatment of stroke, but on the other hand also wanted tissue destruction is responsible.
  • NMDA-dependent glutamate receptors (Liberatore GT, Samson A, Bladin C, Schleuning WD, Medcalf RL: Vampire bat salivary plasminogen activator (desmoteplase): a unique fibrinolytic enzyme that does not promote neurodegeneration, Stroke 2003; 34 (2): 537-543).
  • a proteolytic cleavage of the NMDA receptor by t-PA could be responsible for this effect (Nicole O, Docagne F, Ali C, Margaill I, Carmeliet P, MacKenzie ET, et al: The proteolytic activity of tissue-plasminogen activator enhances NMDA receptor -mediated signaling.Nat Med 2001; 7 (1): 59-64).
  • the plasminogen activator has a modified Kringel domain, preferably a Kringel 2 domain modified in comparison to the native t-PA. This can be completely or partially deleted so that the lysine binding capacity is reduced.
  • the K2 domain - or regions which are essentially homologous in terms of function or structure - can advantageously be modified in such a way that the lysine residues no longer bind or only bind with low affinity.
  • the plasminogen activator that can be used according to the invention is modified t-PA.
  • the plasminogen activating factors which can be used according to the invention show, owing to their lack of or reduced lysine binding capacity, an increased fibrin selectivity and a reduced activatability by fibrinogen or ⁇ -amyloid. This reduced fibrinogen activatability is probably the cause of the fibrin selectivity.
  • a high fibrin selectivity of the piasminogen activator is particularly important in the treatment of stroke, since, for example, the native t-PA can be activated via the fibrinogen that overcomes the damaged blood-brain barrier and the glutamate can be activated via the subsequent activation of the NMDA receptors mediated excitotoxicity stimulated.
  • the reduction in the fibrinogen activatability of the piasminogen activator according to the invention accordingly also leads to reduced neurotoxicity.
  • DSPA is a plasminogen activator that was originally isolated from the saliva of the vampire bat (see US Pat. No. 6,008,019; EP 0 383 417).
  • the DSPA can be isolated in four isoforms, of which the DSPA ⁇ l can be produced recombinantly via CHO cells.
  • DSPA only has a kinkle domain.
  • This domain corresponds functionally and structurally to the K1 domain of the t-PA rather than the K2 domain and has no lysine binding sites (Bringmann P, Gruber D, Liese A, Toschi L, Krhartschmar J, Schleuning WD, Donner P: Structural features mediating fibrin selectivity of vampire bat plasminogen activators; Journal of Biological Chemistry 1995; 270 (43): 25596-25603). It is therefore spoken in the literature in part that DSPA has no Kringel 2 domains.
  • DSPA is always available as a single-chain molecule because there is no plasma activation site as in t-PA.
  • DSPA shows 45,000-fold stimulation of activity in the presence of fibrin compared to t-PA, according to Gardell SJ, Duong LT, Diehl, York JD, Hare TR, Register RB, Jacobs JW, Dixon RA, Friedman PA (isolation, characterization and c-DNA cloning of a vampire bat salivary plasminogen activator: Journal of Biological Chemistry 1989; 264 (30): 17947-17952) is 205.
  • FIG. 2b A schematic representation of the structure of the DSPA is added as FIG. 2.
  • FIG. 2b A comparison of the amino acid sequence of the t-PA with that of the DSPA is shown in FIG. 2b.
  • Bakker et al. made various modifications of the t-PA, such as two deletion mutants lacking either the Kringel-2, the finger domain, or the epidermal growth factor domain. These mutations were also combined and in some cases additionally provided with a point mutation in Kringel-2, namely with the substitution D236N. This targeted amino acid substitution leads to an exchange of the Asp in position 236 to asparagine and thus to a deletion of the lysine binding site (LBS in the K2 domain). To provide the mutation, reference is made to the publication by Bakker et al. including the references cited therein.
  • Bakker et al. were able to show in their investigations that the occupation of the lysine binding site in the K2 domain with EACA ( ⁇ -aminocaproic acid) significantly weakened the binding of the native t-PA to fibrin. The same applies to the modification of the LBS with the substitution D236N, whereby this weakening was less.
  • the deletion mutant which consisted only of the K2 domain and the proteolytic C terminus, was still bound to fibrin, albeit slightly. Only the K2P mutation with deleted LBS no longer showed any fibrin binding.
  • Example 1 confirm the different affinities of DSPA 1 and recombinant human t-PA for the cofactors amyloid ⁇ (1-42) and fibrinogen and fibrin.
  • the kinetic parameters kcat and Km as well as the ratio kcat / Km were determined for each plasminogen activator / CoFactor combination.
  • the efficiency of the DSPA 1 for plasminogen activation was approximately 100 times lower than that of the t-PA, while both were equally efficient in the presence of fibrin.
  • the DSPA ⁇ l was about 30 times less effective than t-PA. This data gives a ratio of the efficiency of fibrin as a cofactor to the cofactors fibrinogen or ⁇ -amyloid at a physiological plasminogen concentration of 2 ⁇ M of 480 for DSPA ⁇ l and 16 for human t-PA.
  • the plasminogen-activating factor used according to the invention is - as already stated above - characterized in that it has no or a modified lysine binding site on Kringel-2.
  • the Kringel-2 is deleted.
  • the plasminogen activator used - in particular the modified t-PA - can be modified by deleting the KringeI-1 such that receptor binding of the t-PA is no longer possible.
  • the tissue plasminogen activator is therefore no longer metabolized in the native way via the liver, so that the in vivo half-life is extended (Rijken DC, Otter M, Kuiper J, from Berkel TJC: Receptor-mediated endocytosis of tissue-type plasminogen activator (t-PA) by liver cells. Thromb. Res. 1990; Supel. X: 63-71).
  • Kringle-1 is known from the reteplase and is known, for example, from Martin U, Bader R, Böhm E, Kohnert U, from Möllendorf E, Fischer S, Sponder G. (BM 06.022: A Novel recombinant plasminogen activator. Cardiovascular drug reviews 1993; 11: 299-311). Mutants with a deletion of amino acids 4-176 are used for this. By extending the half-life, a bolus application of the piasminogen activator is possible.
  • the plasminogen activator which can be used according to the invention is based on one of the amino acid sequences shown in FIGS. 3, 11 or 13.
  • This is a modified t-PA with a deleted curl 2 and a structural change in the sequence region of the activation cleavage site of the t-PA.
  • This change can either consist in the substitution of only the amino acids R and I of the activation cleavage site (for example by HS; see sequence according to FIG. 3) or may also relate to adjacent amino acids (for example substitution of FRIK by LHST in the sequence in accordance with FIG. 11) ).
  • the latter modification corresponds to the structure of the native DSPA at this point.
  • the deletion of the curl 2 in the t-PA is accordingly selected such that a transition area to the structure below is created which corresponds to the comparable structure of the DSPA.
  • the transition region between the remaining curl 1 domain of the modified t-PA and the subsequent cysteine bridge is accordingly advantageously formed by the sequence SKAT.
  • the sequence SKAT can be found in the DSPA between the curling and the cysteine bridge.
  • FIG. 12 A comparison between t-PA and the two described embodiments according to the invention is shown in FIG. 12 (multiple sequence alignment).
  • a plasminogen activator according to FIG. 13 is used.
  • proteins according to the invention which have sequences which are homologous or partially identical to the amino acid sequences shown in FIGS. 3, 11 and 13. Homologies or identities of at least 70, preferably between 80 and 95% are preferred. These homologous or identical proteins show the activity of a plasminogen-activating factor (preferably shown as release of the pNA) and cause the lysis of a blood clot in vitro (see example 3).
  • a plasminogen-activating factor preferably shown as release of the pNA
  • the plasminogen activator that can be used according to the invention can be characterized by a lack of finger and epidermal growth factor domains. be drawn. This deletion significantly reduces the binding to the receptors of the liver and extends the half-life even further (Larson GR, Timony GA, Horgan PG, Barone KM, Henson KS, Angus LB, Stoudemire JB: Protein engineering of novel plasminogen activators with increased thrombolytic potency in rabbits relative to activase. Journal of biological Chemistry 1991; 266: 8156-8161; Smalling RW: Molecular biology of plasminogen activators: what are the clinical implications of drug design? Am J Cardiol 1996; 78 (suppl. 12): 2 - 7).
  • the plasminogen activators used according to the invention can also be modified only selectively by directed mutagenesis.
  • rt-PA-TNK tenecteplase
  • three mutations at the binding site of the plasminogen activator inhibitor on the one hand and at the binding site via which t-PA binds to the liver cells on the other hand increase the lead to catalytic activity while at the same time reducing PAI inactivability.
  • TNK- tissue plasminogen activators in myocardial infarction (TIMI) 10 Results of the initial patients in the TIMI 10 pilot - a phase 1, pharmacokinetics trial, Circulation 1995; 92 (suppl.): 1-415).
  • t-PA Recombinant t-PA (Actilyse® supplied by Paion, batch no. 102572) 10 mg dissolved in 10 ml sterile water to a final concentration of 1 mg / ml.
  • Human fibrinogen essentially plasminogen-free, was purchased from Sigma (Catalog No. F4883, Lot 12K7620). 25 mg was dissolved in 25 ml PCLA buffer to a final concentration of 1 mg / ml.
  • Flavigen.pli D-but-CHT-Lys-P-Nitroanelin-DHCL from Biopool (catalog no.101353, batch 1512016). 100 ⁇ M was dissolved in 50 ml PLCR buffer to a final concentration of 2 milimolar.
  • PLC buffer Jones 1990: 0.1 M NaCl.2H 2 O (Acros Catalog No. 20779); 0.03 M NaHCO 3 (Acros Catalog No. 21712); 4mM KCI (Fluka, Catalog No. 60130); 1mM CaCl 2 .2H 2 (Acros, Catalog No. 207780); 1mM Na 2 HPO 4 .2H 2 O (Fluka, Catalog No. 71638); 0.3 mM MgCl 2 .6H 2 0 (Acros, Catalog No. 197530); 0.4 mM MgSO 4 .7H 2 0 (Fluka, Catalog No. 63,140th); 20mM HEPES (Applichen, Catalog No. A1969); 0.01% Polysorbate 80 (Fluka, Catalog No. 93781)
  • the assay for plasminogen activation was carried out on microtiter plates at a total volume of 0.15 ml as described by Bringma ⁇ n et al. (loc. cit.).
  • the regents were added as follows:
  • the final concentration was as follows:
  • Plasminogen activator (t-PA or DSPA ⁇ l): 0.5 nM; Glu-plasminogen; 0.0625, 0.125, 0.25, 0.5, 1, 2, 4 and 8 ⁇ M; FlavigenPli: 1mM and cofactors: 100 ⁇ g / ml.
  • the cofactors amyloid ⁇ (1-42), fibrinogen and fibrin were compared with the control without cofactor.
  • the reaction mixture contained a further 0.13 units / ml of human thrombin.
  • optical density at 405 nm and 490 nm was measured at regular intervals.
  • the optical densities at 490 nm were subtracted from the optical densities at 405 nm to eliminate the differences due to the liquid movement. All experiments were carried out in three repetitions with each plasminogen concentration and plasminogen activator concentration.
  • plasminogen activators Optical density versus time curves of four different combinations of plasminogen activators and cofactors.
  • plasmogenogen concentrations 8; 4; 2; 1 ; 0.5; 0.25; 0.125 and 0.0625 ⁇ M are used.
  • Table 1 Values for kcat / Km for the activation of plasminogen by recombining human T-PA or DSPA ⁇ l with ß-amyloid (1 to 42), fibrinogen or fibrin as cofactors.
  • the prion diseases, the spongiform encephalopathies are fatal neurodegenerative phenomena, which are characterized by an accumulation of the abnormal isoform of the prion protein (PrP sc ) in amyloid deposits.
  • the normal prion protein (PrP c ) has been expressed in many tissues, especially in the brain, where it is concentrated in the synapses.
  • the mechanism of the conformational change from PrP c to PrP sc is still poorly understood, as is the mechanism that leads to neurodegeneration via the formation of PrP sc .
  • One possible mechanism could be the influence of PrP sc on plasminogen activation by t-PA.
  • PrP PrP to stimulate plasminogen activation
  • PrP conformation which was influenced by the presence or absence of Cu 2+ .
  • the latter form contains a large number of ß structures which correspond to PrP sc isoform and stimulate plasminogen activation.
  • PrP-stimulated plasminogen activation also includes the binding of t-PA apo-PrP (ie PrP without Cu 2+ ).
  • the structures required for this binding have not yet been evaluated in detail. However, the binding is specific because it was prevented by DFP-inactivated t-PA.
  • PrP approaches used in these studies were derived from recombinant murine PrP from E. coli. Following purification of the His labeled PrP, the protein folds again in the absence or presence of copper chloride to form either a holo-PrP or an apo-PrP. Aggregated forms of these proteins are created by quickly dissolving them in water. The protein was collected by centrifugation. The material is resistant to Proteinase-K digestion. A mutant form of ⁇ 51-90, which does not include the Cu 2+ binding site, was prepared in the same way.
  • PrP PrP
  • Lys plasmonogen 25 nm
  • DSPA DSPA (0.25 nm).
  • the plasminogen activation is determined by the hydrolysis of the plasmin-specific dye Hd-Val-Leu-Lys-7-amido-4-methylcoumarin at 37 ° C using the SPECTRAmax Gemini lourescence microplate reader.
  • the controls of these experiments included sc-t-PA, as a positive control for the effect of PrP on plasminogen activation, and Firbin fragments as a positive control for stimulation of t-PA and DSPA activity.
  • the specificity of the plasminogen binding was determined by competition with DFP-inactivated t-Pa.
  • the lysine analog EACA was also used as a competitive inhibitor.
  • Recombinant humanDSPA baculovirus DNA was produced using the Bac-to-Bac system (Invitrogen) and the purified virus DNA was transfected into Sf9 insect cells.
  • the recombinant baculovirus produced was amplified using Sf-9 cells.
  • HumDSAP was expressed with high five insect cells in serum-free medium in suspension culture. The cell culture supernatant with the recombinant protein was frozen at -20 ° C. until further processing.
  • the cell culture supernatant was slowly thawed at RT on a shaker and centrifuged for 1 hour at 4000xg.
  • the protein solution (1.51) was equilibrated to pH 6.0 with 50 mM ammonium acetate, loaded onto an SP-Sepharose XL (Amersham) ion exchange column (350 ml bed volume) and washed with 5 column volumes of 50 mM ammonium acetate solution.
  • the bound protein was eluted in a gradient of 0-100% 1 M ammonium acetate pH 6 over 10 column volumes.
  • the activity assay used is a determination of the rate at which a plasminogen activator converts the colorless substrate S-2288 into a colored product.
  • the assay represents a standard method for determining the proteolytic activity of plasminogen activating factors.
  • To display the activity the release of a chromogen (p-nitroaniline, pNA) is determined by the test substance.
  • the Chromogenix company offers the Chromogen S-2288.
  • reaction rate is not determined directly (e.g. in the catalytic converter), but is compared with a standard that is defined as 100% activity.
  • reaction rate measurement was carried out in a buffer which had the following composition: 25mM Tris-HCl pH8 / 0.1% albumin / 100mM NaCl / 1.1mM Glycine / 1.2mM mannitol / 2.5mM S-2288
  • the measurement was carried out in a 96-hole plate with blank values, standards and samples with different protein concentrations.
  • FIG. 14 The activity of the fractions (expressed in S-2288 activity) is shown in FIG. 14.
  • the silver-colored gels can be seen in FIGS. 15 a and 15 b.
  • FIG. 16a and 16b show the western blots.
  • the protein detected in the Western blot corresponds to the protein marked with the arrow head in the silver-colored gel.
  • Clot lysis (thrombolytic activity) The residue of the humaDSPA-containing fraction B6 was dissolved in 15 ml PBS and centrifuged at 4000xg for 15 min. This fraction was chosen because it showed the greatest purity. An aliquot was taken and used to determine activity. The catalytic activity of this sample was roughly comparable to the catalytic activity of 5 ⁇ g / ml activase.
  • the blood clots used originate from normal blood sampling 24 hours earlier and each resulted from 2 ml of blood that was transferred to polypropylene tubes and curdled under natural conditions.
  • the lysis was carried out in PBS. ,
  • the humaDSPA-containing fraction B6 (from SPXL-Sepharose capture step) was first lyophilized. The residue was dissolved in 15 ml of PBS and centrifuged for 15 min / 4000 g. Before the blood clot was added, a sample was taken for an S-2288 activity determination. The catalytic activity of this sample essentially corresponded to the catalytic activity of 5 ⁇ g / ml activase.
  • FIGS. 17a to 17d show the results of the clot lysis in the time course of 0h, 3h, 4h and 24h after addition of the humDSPA.
  • the left test approach shows the control.
  • PBS with humaDSPA is contained in the amount described above.
  • the blood cells slowly fall out of the clot that dissolves. After 24 hours, a kind of network remains that can be recognized after just 4 hours.
  • detached blood cells were aspirated with a syringe so that the structure of the remaining clot was visible.
  • fibrinolysis slows down when the clot no longer hangs in its own lysate. Therefore, a clot is still present after 24 hours.
  • the humDSPA shows a significant increase in activity, since at least four times the amount of DSPA must be used for the dissolution of a clot of the same size by DSPA.

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Abstract

L'invention concerne l'utilisation d'activateurs plasminogènes à capacité de liaison lysine réduite pour traiter une maladie thrombotique.
EP04765139A 2003-09-12 2004-09-13 Activateurs plasminogenes a capacite de liaison lysine reduite Withdrawn EP1664293A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP10011604A EP2368984A1 (fr) 2003-09-12 2004-09-13 Activateurs plasminogenes a capacite de liaison lysine reduite

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10342518A DE10342518A1 (de) 2003-09-12 2003-09-12 Plasminogen-Aktivatoren mit verringerter Lysin-Bindungskapazität
PCT/EP2004/010220 WO2005026341A2 (fr) 2003-09-12 2004-09-13 Activateurs plasminogenes a capacite de liaison lysine reduite

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Publication Number Publication Date
EP1664293A2 true EP1664293A2 (fr) 2006-06-07

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US8430749B2 (en) 2001-08-10 2013-04-30 Igt Dynamic casino tracking and optimization
US7946917B2 (en) 2001-08-10 2011-05-24 Igt Flexible loyalty points programs
DE10153601A1 (de) 2001-11-02 2003-05-22 Paion Gmbh DSPA zur Behandlung von Schlaganfall
DE10342518A1 (de) 2003-09-12 2005-05-12 Paion Gmbh Plasminogen-Aktivatoren mit verringerter Lysin-Bindungskapazität
IL190184A0 (en) * 2008-03-16 2009-02-11 Hadasit Med Res Service tPA MUTANT IN THE TREATMENT OF ACUTE BRAIN INJURY AND NEURODEGENERATIVE DISORDERS

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EP0383417A1 (fr) * 1989-02-13 1990-08-22 Schering Aktiengesellschaft Activateur du plasminogène salivaires de chauve-souris vPA-alpha 1

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EP0383417A1 (fr) * 1989-02-13 1990-08-22 Schering Aktiengesellschaft Activateur du plasminogène salivaires de chauve-souris vPA-alpha 1

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WO2005026341A3 (fr) 2005-09-01
CN1849394A (zh) 2006-10-18
DE10342518A1 (de) 2005-05-12
EA200600573A1 (ru) 2006-08-25
MXPA06002756A (es) 2007-08-24
BRPI0413718A (pt) 2006-10-17
IL173953A0 (en) 2006-07-05
CA2537696A1 (fr) 2005-03-24
NZ545825A (en) 2009-08-28
EP2368984A1 (fr) 2011-09-28
ZA200602716B (en) 2007-07-25
JP2007505079A (ja) 2007-03-08
EA011156B1 (ru) 2009-02-27
EA200801861A1 (ru) 2010-06-30
NO20061646L (no) 2006-04-11
WO2005026341A2 (fr) 2005-03-24
AU2004272731A1 (en) 2005-03-24

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