EP1809089A1 - Mykorrhiza-pilzpräparate , deren herstellung und deren verwendung für die inokulierung von pflanzen - Google Patents
Mykorrhiza-pilzpräparate , deren herstellung und deren verwendung für die inokulierung von pflanzenInfo
- Publication number
- EP1809089A1 EP1809089A1 EP05798802A EP05798802A EP1809089A1 EP 1809089 A1 EP1809089 A1 EP 1809089A1 EP 05798802 A EP05798802 A EP 05798802A EP 05798802 A EP05798802 A EP 05798802A EP 1809089 A1 EP1809089 A1 EP 1809089A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- mill
- mycorrhizal
- preparation
- particle size
- arbuskel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 241000233866 Fungi Species 0.000 title claims abstract description 15
- 238000004519 manufacturing process Methods 0.000 title description 4
- 238000011081 inoculation Methods 0.000 title description 3
- 239000008187 granular material Substances 0.000 claims abstract description 10
- 241000235504 Rhizophagus intraradices Species 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 14
- 239000000758 substrate Substances 0.000 claims description 10
- 239000002245 particle Substances 0.000 claims description 9
- 239000004927 clay Substances 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 7
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 6
- 230000002538 fungal effect Effects 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 3
- 239000010451 perlite Substances 0.000 claims description 3
- 235000019362 perlite Nutrition 0.000 claims description 3
- 239000011435 rock Substances 0.000 claims description 3
- 241000235503 Glomus Species 0.000 claims description 2
- 229960000892 attapulgite Drugs 0.000 claims description 2
- 230000004720 fertilization Effects 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims description 2
- 229910052625 palygorskite Inorganic materials 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 239000010455 vermiculite Substances 0.000 claims description 2
- 229910052902 vermiculite Inorganic materials 0.000 claims description 2
- 235000019354 vermiculite Nutrition 0.000 claims description 2
- 230000035939 shock Effects 0.000 claims 1
- 239000013589 supplement Substances 0.000 claims 1
- 241001287359 Glomus sp. Species 0.000 abstract description 4
- 239000002054 inoculum Substances 0.000 abstract description 4
- 241000196324 Embryophyta Species 0.000 description 13
- 239000011148 porous material Substances 0.000 description 3
- 239000012043 crude product Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 238000003801 milling Methods 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 239000001707 (E,7R,11R)-3,7,11,15-tetramethylhexadec-2-en-1-ol Substances 0.000 description 1
- 241000773655 Ambispora fennica Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- BLUHKGOSFDHHGX-UHFFFAOYSA-N Phytol Natural products CC(C)CCCC(C)CCCC(C)CCCC(C)C=CO BLUHKGOSFDHHGX-UHFFFAOYSA-N 0.000 description 1
- 241000923606 Schistes Species 0.000 description 1
- 235000012308 Tagetes Nutrition 0.000 description 1
- 241000736851 Tagetes Species 0.000 description 1
- HNZBNQYXWOLKBA-UHFFFAOYSA-N Tetrahydrofarnesol Natural products CC(C)CCCC(C)CCCC(C)=CCO HNZBNQYXWOLKBA-UHFFFAOYSA-N 0.000 description 1
- BOTWFXYSPFMFNR-OALUTQOASA-N all-rac-phytol Natural products CC(C)CCC[C@H](C)CCC[C@H](C)CCCC(C)=CCO BOTWFXYSPFMFNR-OALUTQOASA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011362 coarse particle Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- BOTWFXYSPFMFNR-PYDDKJGSSA-N phytol Chemical compound CC(C)CCC[C@@H](C)CCC[C@@H](C)CCC\C(C)=C\CO BOTWFXYSPFMFNR-PYDDKJGSSA-N 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 239000010454 slate Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/28—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture specially adapted for farming
Definitions
- the present invention relates to arbore-forming mycorrhizal fungal preparations which can be used to improve plant inoculation.
- the inoculation of plants with mycorrhizal fungi serves as a useful complement to fertilization and crop protection in agriculture, especially in the cultivation of vegetables, fruits and flowers, as well as in forestry.
- AM fungi Arbuscone-forming mycorrhizal fungi enter into a beneficial symbiosis with a large number of plants. Colonization of the root cells of a plant with such fungi results in the development of a more extensive, better net-like, intergrown root system. Thus, mushroom hyphae grow outside the normal root ball into a zone further out, in which nutrients, such as in particular phosphorus, are normally inaccessible to the plant. It has been shown that AM fungi cause a significant improvement in plant growth (see, for example, S. Gianinazzi, H, Schüepp, J, M. Barea, K, Haselwandter, "Mycorrhizal technology in Agriculture", Birkhäuser 2002, E.
- AM mushroom preparations have not yet gained much importance in agriculture, particularly in the cultivation of vegetables and fruits, or in forestry. The reason for this is partly due to the lack of availability of larger amounts of applicator properly flawless preparations.
- AM fungi are obligate phytotrophs that can not multiply without host plant. Their production must therefore take place in a combined system.
- AM mushroom ocellulara in particular Glomus sp. , prepared by culturing host plants in pots or beds on inert substrates (see, for example, H, Baltruschat et al., EP 0 163 840, 1985).
- Such culturing methods have the advantage of simplicity and enable the production of a large amount of AM fungal spores.
- All porous and biologically inert materials are suitable as substrates for cultivation.
- a relatively coarse particle size of about 1 to 8 mm and pore diameters of the same order of magnitude as the spore dimensions have proven to be particularly suitable.
- Expanded clay, swelling schist, volcanic rock or perlite have these properties. The use of smaller particle sizes or less porous materials leads to lower process yields.
- AM fungal preparations are prepared at about 100-200 infectious units per milliliter (see Gianinazzi, supra). Depending on the grain size of the substrate, these units are split into only 5 to 20 grains per milliliter. It follows that, despite a high number of active spores and hyphae, their spatial distribution around the plant roots is unsatisfactory. The effective and economically justifiable application of AM fungal preparations thus remains limited.
- the aim of the invention is to solve the problem of spatially limited application of AM mushroom preparations. H2005 / 000648
- a crude cocum is obtained by cultivation of AM fungi, such as Glomus sp. , in particular Glomus Intraradlces, produced on a porous granule as substrate, as described by Baltruschat (see above).
- AM fungi such as Glomus sp. , in particular Glomus Intraradlces
- Expanded clay or attapulgite or expanded slate or volcanic rock or perlite or vermiculite or a mixture of two or more of these materials is expediently used as porous granules for this purpose.
- the particle size of this porous granules is expediently about 1 and 6 mm.
- the Rohinokulum contains spores, hyphae, as well as fine root remnants of the host plants.
- Analytical Biological Testing according to Sieverding reveals a probable number of infectious units of typically 300 to 600 per gram. In highly productive plants, this number may even exceed 2000. Since these units are buried in the pores of the substrate, isolated spores of the AM fungi, such as Glomus sp., Especially Glomus intraradices, can not be detected under the microscope.
- the Rohinokulum is comminuted so gently that the active spores are not destroyed.
- This can be done by using an impact / impact rather than a friction milling process.
- Suitable types of impact / impact mills for this purpose are, in particular, hammer mills, impact mills or air jet mills ("jet mills").
- the operational parameters of the mill must be adjusted so that the particle size of the milled product is in a range of about 100-500 ⁇ m, preferably about 100-250 ⁇ m. In particular, therefore, the rotational speed must be set accordingly and the discharge sieve selected accordingly. Under the microscope, up to 1000 free spores or spore groups per gram can be counted in the ground product.
- the minced product obtained according to the invention can be used directly as a solid for inoculating plants with AM fungi, or it can be standardized to a standard activity by dilution with a conventional inert substance. It can also be formulated with customary formulation auxiliaries to form a water-suspendable preparation.
- An AM fungal inoculum was prepared in expanded clay granules with a particle size of 2-4 mm after Baltruschat (see above).
- the resulting mycorrhizal-containing product was milled with various mills under various conditions. The number of free spores or spore groups in the millbase was determined under the microscope.
- Plastic plant pots (round, height 6.5 cm, diameter: 9 cm at the top, 6 cm at the bottom) were filled with a mixture of on the one hand 500 mg or 1000 mg of the product ground in accordance with Example 1 with the pilot hammer mill No. 1 or the crude product Example 1 and on the other hand, a substrate consisting of seed soil (Floradur-Fein) and sterilized Sand in the ratio 1: 1. Only pots filled with substrate (potting soil / sand) served as control,
- the pots were poured normally with tap water and fertilized once a week with a Wuxal top N solution (concentration 0.05% during the first 3 weeks, 0.1% after).
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
Description
Claims
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH18282004 | 2004-11-05 | ||
| PCT/CH2005/000648 WO2006047903A1 (de) | 2004-11-05 | 2005-11-04 | Mykorrhiza-pilzpräparate , deren herstellung und deren verwendung für die inokulierung von pflanzen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP1809089A1 true EP1809089A1 (de) | 2007-07-25 |
Family
ID=34974196
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP05798802A Withdrawn EP1809089A1 (de) | 2004-11-05 | 2005-11-04 | Mykorrhiza-pilzpräparate , deren herstellung und deren verwendung für die inokulierung von pflanzen |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US20080064598A1 (de) |
| EP (1) | EP1809089A1 (de) |
| WO (1) | WO2006047903A1 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL1036130C (nl) * | 2008-10-29 | 2010-05-03 | Arcadis Nederland B V | Werkwijze en samenstelling voor geschikt maken van landbouw- of natuurgebieden. |
| ES2343235B8 (es) * | 2009-01-23 | 2011-12-28 | Consejo Superior De Investigaciones Cientificas (Csic) | Hongo formador de micorrizas arbusculares y su uso para estimular el crecimiento de plantas. |
| MX2010010894A (es) * | 2010-10-04 | 2011-07-11 | Biokrone S A De C V | Proceso de elaboracion de granulos peletizados a base de hongos endomicorrizicos recubiertos con arcillas minerales y su composicion. |
| FR2969464B1 (fr) * | 2010-12-23 | 2015-10-23 | Inst Rech Developpement Ird | Nouvelles compositions d'inocula fongiques, leur procede de preparation et leur application a l'amelioration de la croissance des cultures |
| CA2976207A1 (en) * | 2014-02-17 | 2015-08-20 | Agpw, Llc | Porous matrices for culture and formulation of agricultural biopesticides and chemicals |
| BE1023546B1 (fr) * | 2015-04-07 | 2017-04-28 | Sous Les Fraises Sas | Supports pour cultiver un organisme, utilisations desdits supports et methodes de culture et d'epuration utilsant ces supports |
| EP4281427A4 (de) * | 2021-01-21 | 2025-04-09 | Carbo Ceramics Inc. | Pellets mit landwirtschaftlichen behandlungsmaterialien und verfahren zur herstellung davon |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2119617A (en) * | 1982-04-22 | 1983-11-23 | Nat Res Dev | Mycorrhizal seed pellets |
| DE3416315A1 (de) * | 1984-05-03 | 1985-11-07 | Chemische Werke Hüls AG, 4370 Marl | Herstellung und verwendung von adsorbentien zur inokulation von pflanzen mit vesikulaer-arbuskulaeren mykorrhizapilzen |
| DE3932746A1 (de) * | 1989-09-30 | 1991-04-11 | Weritz Juergen Dipl Agr Ing | Traegermaterial fuer die produktion und applikation von va-mykorrhizapilzpropagationsorganen sowie verfahren fuer dessen weiterverarbeitung |
| ATE117282T1 (de) * | 1990-11-08 | 1995-02-15 | Shell Int Research | Va-mykorrhizapilze enthaltende wasserdispergierbare granulate, ihre herstellung und ihre verwendung. |
| FR2678281A1 (fr) * | 1991-06-26 | 1992-12-31 | Agronomique Inst Nat Rech | Moyens pour ameliorer la croissance des plantes. |
| US20020103083A1 (en) * | 2000-08-11 | 2002-08-01 | Harman Gary E. | Promoting deeper root development, reducing nitrogen fertilizer usage, imparting drought resistance, and increasing tolerance to adverse soil conditions in plants |
| US6759232B2 (en) * | 2002-04-29 | 2004-07-06 | Wen-Kai Wang | Method of facilitating mass production and sporulation of arbuscular mycorrhizal fungi aseptic in vitro |
-
2005
- 2005-11-04 EP EP05798802A patent/EP1809089A1/de not_active Withdrawn
- 2005-11-04 WO PCT/CH2005/000648 patent/WO2006047903A1/de not_active Ceased
- 2005-11-04 US US11/718,759 patent/US20080064598A1/en not_active Abandoned
Non-Patent Citations (1)
| Title |
|---|
| See references of WO2006047903A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2006047903A1 (de) | 2006-05-11 |
| US20080064598A1 (en) | 2008-03-13 |
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Legal Events
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| DAX | Request for extension of the european patent (deleted) | ||
| GRAP | Despatch of communication of intention to grant a patent |
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| STAA | Information on the status of an ep patent application or granted ep patent |
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| 18D | Application deemed to be withdrawn |
Effective date: 20140520 |