EP1809089A1 - Preparations fongiques de mycorhize, leur production et leur utilisation pour inoculer des plantes - Google Patents

Preparations fongiques de mycorhize, leur production et leur utilisation pour inoculer des plantes

Info

Publication number
EP1809089A1
EP1809089A1 EP05798802A EP05798802A EP1809089A1 EP 1809089 A1 EP1809089 A1 EP 1809089A1 EP 05798802 A EP05798802 A EP 05798802A EP 05798802 A EP05798802 A EP 05798802A EP 1809089 A1 EP1809089 A1 EP 1809089A1
Authority
EP
European Patent Office
Prior art keywords
mill
mycorrhizal
preparation
particle size
arbuskel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP05798802A
Other languages
German (de)
English (en)
Inventor
Michel De Rougement
Meinolf Stützer
Angelika Spindler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mycosym International AG
Original Assignee
Mycosym International AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mycosym International AG filed Critical Mycosym International AG
Publication of EP1809089A1 publication Critical patent/EP1809089A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/10—Mycorrhiza; Mycorrhizal associations
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/28—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture specially adapted for farming

Definitions

  • the present invention relates to arbore-forming mycorrhizal fungal preparations which can be used to improve plant inoculation.
  • the inoculation of plants with mycorrhizal fungi serves as a useful complement to fertilization and crop protection in agriculture, especially in the cultivation of vegetables, fruits and flowers, as well as in forestry.
  • AM fungi Arbuscone-forming mycorrhizal fungi enter into a beneficial symbiosis with a large number of plants. Colonization of the root cells of a plant with such fungi results in the development of a more extensive, better net-like, intergrown root system. Thus, mushroom hyphae grow outside the normal root ball into a zone further out, in which nutrients, such as in particular phosphorus, are normally inaccessible to the plant. It has been shown that AM fungi cause a significant improvement in plant growth (see, for example, S. Gianinazzi, H, Schüepp, J, M. Barea, K, Haselwandter, "Mycorrhizal technology in Agriculture", Birkhäuser 2002, E.
  • AM mushroom preparations have not yet gained much importance in agriculture, particularly in the cultivation of vegetables and fruits, or in forestry. The reason for this is partly due to the lack of availability of larger amounts of applicator properly flawless preparations.
  • AM fungi are obligate phytotrophs that can not multiply without host plant. Their production must therefore take place in a combined system.
  • AM mushroom ocellulara in particular Glomus sp. , prepared by culturing host plants in pots or beds on inert substrates (see, for example, H, Baltruschat et al., EP 0 163 840, 1985).
  • Such culturing methods have the advantage of simplicity and enable the production of a large amount of AM fungal spores.
  • All porous and biologically inert materials are suitable as substrates for cultivation.
  • a relatively coarse particle size of about 1 to 8 mm and pore diameters of the same order of magnitude as the spore dimensions have proven to be particularly suitable.
  • Expanded clay, swelling schist, volcanic rock or perlite have these properties. The use of smaller particle sizes or less porous materials leads to lower process yields.
  • AM fungal preparations are prepared at about 100-200 infectious units per milliliter (see Gianinazzi, supra). Depending on the grain size of the substrate, these units are split into only 5 to 20 grains per milliliter. It follows that, despite a high number of active spores and hyphae, their spatial distribution around the plant roots is unsatisfactory. The effective and economically justifiable application of AM fungal preparations thus remains limited.
  • the aim of the invention is to solve the problem of spatially limited application of AM mushroom preparations. H2005 / 000648
  • a crude cocum is obtained by cultivation of AM fungi, such as Glomus sp. , in particular Glomus Intraradlces, produced on a porous granule as substrate, as described by Baltruschat (see above).
  • AM fungi such as Glomus sp. , in particular Glomus Intraradlces
  • Expanded clay or attapulgite or expanded slate or volcanic rock or perlite or vermiculite or a mixture of two or more of these materials is expediently used as porous granules for this purpose.
  • the particle size of this porous granules is expediently about 1 and 6 mm.
  • the Rohinokulum contains spores, hyphae, as well as fine root remnants of the host plants.
  • Analytical Biological Testing according to Sieverding reveals a probable number of infectious units of typically 300 to 600 per gram. In highly productive plants, this number may even exceed 2000. Since these units are buried in the pores of the substrate, isolated spores of the AM fungi, such as Glomus sp., Especially Glomus intraradices, can not be detected under the microscope.
  • the Rohinokulum is comminuted so gently that the active spores are not destroyed.
  • This can be done by using an impact / impact rather than a friction milling process.
  • Suitable types of impact / impact mills for this purpose are, in particular, hammer mills, impact mills or air jet mills ("jet mills").
  • the operational parameters of the mill must be adjusted so that the particle size of the milled product is in a range of about 100-500 ⁇ m, preferably about 100-250 ⁇ m. In particular, therefore, the rotational speed must be set accordingly and the discharge sieve selected accordingly. Under the microscope, up to 1000 free spores or spore groups per gram can be counted in the ground product.
  • the minced product obtained according to the invention can be used directly as a solid for inoculating plants with AM fungi, or it can be standardized to a standard activity by dilution with a conventional inert substance. It can also be formulated with customary formulation auxiliaries to form a water-suspendable preparation.
  • An AM fungal inoculum was prepared in expanded clay granules with a particle size of 2-4 mm after Baltruschat (see above).
  • the resulting mycorrhizal-containing product was milled with various mills under various conditions. The number of free spores or spore groups in the millbase was determined under the microscope.
  • Plastic plant pots (round, height 6.5 cm, diameter: 9 cm at the top, 6 cm at the bottom) were filled with a mixture of on the one hand 500 mg or 1000 mg of the product ground in accordance with Example 1 with the pilot hammer mill No. 1 or the crude product Example 1 and on the other hand, a substrate consisting of seed soil (Floradur-Fein) and sterilized Sand in the ratio 1: 1. Only pots filled with substrate (potting soil / sand) served as control,
  • the pots were poured normally with tap water and fertilized once a week with a Wuxal top N solution (concentration 0.05% during the first 3 weeks, 0.1% after).

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

L'invention concerne des préparations fongiques de mycorhize formant des arbuscules. On obtient ces préparations en cultivant un inoculum brut sur un granulat poreux et en fractionnant l'inoculum brut ainsi obtenu méticuleusement de façon à ne pas détruire les spores actives. Ces préparations sont particulièrement adaptées à la fertilisation et à la protection des végétaux en agriculture et en sylviculture. Glomus sp., notamment Glomus intraradices sont des champignons de mycorhize adaptés. L'inoculum brut est fractionné au moyen d'un moulin à pilons et impacts.
EP05798802A 2004-11-05 2005-11-04 Preparations fongiques de mycorhize, leur production et leur utilisation pour inoculer des plantes Withdrawn EP1809089A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH18282004 2004-11-05
PCT/CH2005/000648 WO2006047903A1 (fr) 2004-11-05 2005-11-04 Preparations fongiques de mycorhize, leur production et leur utilisation pour inoculer des plantes

Publications (1)

Publication Number Publication Date
EP1809089A1 true EP1809089A1 (fr) 2007-07-25

Family

ID=34974196

Family Applications (1)

Application Number Title Priority Date Filing Date
EP05798802A Withdrawn EP1809089A1 (fr) 2004-11-05 2005-11-04 Preparations fongiques de mycorhize, leur production et leur utilisation pour inoculer des plantes

Country Status (3)

Country Link
US (1) US20080064598A1 (fr)
EP (1) EP1809089A1 (fr)
WO (1) WO2006047903A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL1036130C (nl) * 2008-10-29 2010-05-03 Arcadis Nederland B V Werkwijze en samenstelling voor geschikt maken van landbouw- of natuurgebieden.
ES2343235B8 (es) * 2009-01-23 2011-12-28 Consejo Superior De Investigaciones Cientificas (Csic) Hongo formador de micorrizas arbusculares y su uso para estimular el crecimiento de plantas.
MX2010010894A (es) * 2010-10-04 2011-07-11 Biokrone S A De C V Proceso de elaboracion de granulos peletizados a base de hongos endomicorrizicos recubiertos con arcillas minerales y su composicion.
FR2969464B1 (fr) * 2010-12-23 2015-10-23 Inst Rech Developpement Ird Nouvelles compositions d'inocula fongiques, leur procede de preparation et leur application a l'amelioration de la croissance des cultures
CA2976207A1 (fr) * 2014-02-17 2015-08-20 Agpw, Llc Matrices poreuses pour la culture et la formulation de pesticides biologiques agricoles et de produits agrochimiques
BE1023546B1 (fr) * 2015-04-07 2017-04-28 Sous Les Fraises Sas Supports pour cultiver un organisme, utilisations desdits supports et methodes de culture et d'epuration utilsant ces supports
EP4281427A4 (fr) * 2021-01-21 2025-04-09 Carbo Ceramics Inc. Pastilles contenant des matériaux de traitement agricole et leurs procédés de fabrication

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2119617A (en) * 1982-04-22 1983-11-23 Nat Res Dev Mycorrhizal seed pellets
DE3416315A1 (de) * 1984-05-03 1985-11-07 Chemische Werke Hüls AG, 4370 Marl Herstellung und verwendung von adsorbentien zur inokulation von pflanzen mit vesikulaer-arbuskulaeren mykorrhizapilzen
DE3932746A1 (de) * 1989-09-30 1991-04-11 Weritz Juergen Dipl Agr Ing Traegermaterial fuer die produktion und applikation von va-mykorrhizapilzpropagationsorganen sowie verfahren fuer dessen weiterverarbeitung
ATE117282T1 (de) * 1990-11-08 1995-02-15 Shell Int Research Va-mykorrhizapilze enthaltende wasserdispergierbare granulate, ihre herstellung und ihre verwendung.
FR2678281A1 (fr) * 1991-06-26 1992-12-31 Agronomique Inst Nat Rech Moyens pour ameliorer la croissance des plantes.
US20020103083A1 (en) * 2000-08-11 2002-08-01 Harman Gary E. Promoting deeper root development, reducing nitrogen fertilizer usage, imparting drought resistance, and increasing tolerance to adverse soil conditions in plants
US6759232B2 (en) * 2002-04-29 2004-07-06 Wen-Kai Wang Method of facilitating mass production and sporulation of arbuscular mycorrhizal fungi aseptic in vitro

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2006047903A1 *

Also Published As

Publication number Publication date
WO2006047903A1 (fr) 2006-05-11
US20080064598A1 (en) 2008-03-13

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