EP1920253A2 - Detection de proteases secretees a partir de micro-organismes pathogenes - Google Patents

Detection de proteases secretees a partir de micro-organismes pathogenes

Info

Publication number
EP1920253A2
EP1920253A2 EP06789202A EP06789202A EP1920253A2 EP 1920253 A2 EP1920253 A2 EP 1920253A2 EP 06789202 A EP06789202 A EP 06789202A EP 06789202 A EP06789202 A EP 06789202A EP 1920253 A2 EP1920253 A2 EP 1920253A2
Authority
EP
European Patent Office
Prior art keywords
protease
substrate
reagent
test sample
color
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP06789202A
Other languages
German (de)
English (en)
Inventor
Xuedong Song
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kimberly Clark Worldwide Inc
Kimberly Clark Corp
Original Assignee
Kimberly Clark Worldwide Inc
Kimberly Clark Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/217,097 external-priority patent/US7504235B2/en
Application filed by Kimberly Clark Worldwide Inc, Kimberly Clark Corp filed Critical Kimberly Clark Worldwide Inc
Publication of EP1920253A2 publication Critical patent/EP1920253A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/521—Single-layer analytical elements
    • G01N33/523—Single-layer analytical elements the element being adapted for a specific analyte

Definitions

  • the diazonium ion may be zwitterionic in that the counterion of the diazonium moiety is covalently bound to the ring system.
  • the ring system of the diazonium ion may be substituted or unsubstituted.
  • the ion may be provided by a variety of suitable diazonium salts, such as diazonium chlorides, diazonium acid sulphates, diazonium alkyl sulphates, diazonium fluoborates, diazonium benzenesulphonates, diazonium acid 1 ,5-naphthalenedisulphonates, and so forth.
  • a substrate is employed.
  • the substrate may be mixed with the test sample prior to application to the device 20.
  • the substrate may be diffusively immobilized on the device 20 prior to application of the test sample.
  • Such pre-application provides a variety of benefits, including the elimination of the need for a subsequent user to handle and mix the reagents with the test sample or a diluent. This is particularly useful in point-of- care applications when the user is not generally a trained lab technician or medical professional.
  • the substrate may be disposed downstream from the sample application zone 24. In this manner, the test sample is capable of mixing with the substrate upon application. Alternatively, the substrate may be positioned upstream from the sample application zone 24. For instance, a diluent may be employed to induce mixing between the substrate and test sample.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention a trait à une trousse d'essai diagnostique pour la détection de la présence ou de l'absence d'une protéase (par exemple, l'aminopeptidase) au sein d'un échantillon d'essai. La trousse d'essai comporte un substrat qui est capable d'être clivé en présence de la protéase pour la libération d'un composé. La trousse comporte également un dispositif d'écoulement latéral comprenant un support chromatographique. Le support chromatographique définit une zone de détection au sein de laquelle est contenu un premier réactif (par exemple, l'ion diazonium) qui est capable de réagir avec le composé pour former un deuxième réactif (par exemple, un composé azoïque aromatique). Le deuxième réactif présente une couleur qui est différente de la couleur du premier réactif. Le dispositif d'écoulement latéral comporte également un matériau absorbant disposé adjacent au support chromatographique, le matériau absorbant recevant l'échantillon d'essai suite à son écoulement à travers le support chromatographique.
EP06789202A 2005-08-31 2006-08-01 Detection de proteases secretees a partir de micro-organismes pathogenes Withdrawn EP1920253A2 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/217,097 US7504235B2 (en) 2005-08-31 2005-08-31 Enzyme detection technique
US11/364,810 US7575887B2 (en) 2005-08-31 2006-02-28 Detection of proteases secreted from pathogenic microorganisms
PCT/US2006/030107 WO2007027353A2 (fr) 2005-08-31 2006-08-01 Detection de proteases secretees a partir de micro-organismes pathogenes

Publications (1)

Publication Number Publication Date
EP1920253A2 true EP1920253A2 (fr) 2008-05-14

Family

ID=37809345

Family Applications (1)

Application Number Title Priority Date Filing Date
EP06789202A Withdrawn EP1920253A2 (fr) 2005-08-31 2006-08-01 Detection de proteases secretees a partir de micro-organismes pathogenes

Country Status (2)

Country Link
EP (1) EP1920253A2 (fr)
WO (1) WO2007027353A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7504235B2 (en) * 2005-08-31 2009-03-17 Kimberly-Clark Worldwide, Inc. Enzyme detection technique
US7935538B2 (en) 2006-12-15 2011-05-03 Kimberly-Clark Worldwide, Inc. Indicator immobilization on assay devices
US20080269707A1 (en) 2007-04-30 2008-10-30 Kimberly-Clark Worldwide, Inc. Lateral Flow Device for Attachment to an Absorbent Article

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999027364A1 (fr) * 1997-11-24 1999-06-03 Quidel Corporation Mise en place du substrat enzymatique et enregistrement du produit dans des dosages immunoenzymatiques en une etape
WO2006006960A1 (fr) * 2004-06-30 2006-01-19 Kimberly-Clark Worldwide, Inc. Detection d'enzymes et d'amines en une seule operation
WO2006006961A1 (fr) * 2004-06-30 2006-01-19 Kimberly-Clark Worldwide, Inc. Detection d'enzymes

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3413077A1 (de) * 1984-04-06 1985-10-17 Miles Laboratories, Inc., Elkhart, Ind. Chromogene aminosaeure- und peptidester, verfahren zu deren herstellung, verwendung dieser verbindungen in analysenverfahren sowie mittel zum nachweis esterolytischer und/oder proteolytischer enzyme
US5571684A (en) * 1994-11-07 1996-11-05 Litmus Concepts, Inc. Assay for proline iminopeptidase and other hydrolytic activities
US7781172B2 (en) * 2003-11-21 2010-08-24 Kimberly-Clark Worldwide, Inc. Method for extending the dynamic detection range of assay devices
US20050220712A1 (en) * 2004-04-01 2005-10-06 Wright Daniel G Method for determining mucosal neutrophil counts in neutropenia patents
US7504235B2 (en) * 2005-08-31 2009-03-17 Kimberly-Clark Worldwide, Inc. Enzyme detection technique

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999027364A1 (fr) * 1997-11-24 1999-06-03 Quidel Corporation Mise en place du substrat enzymatique et enregistrement du produit dans des dosages immunoenzymatiques en une etape
WO2006006960A1 (fr) * 2004-06-30 2006-01-19 Kimberly-Clark Worldwide, Inc. Detection d'enzymes et d'amines en une seule operation
WO2006006961A1 (fr) * 2004-06-30 2006-01-19 Kimberly-Clark Worldwide, Inc. Detection d'enzymes

Also Published As

Publication number Publication date
WO2007027353A2 (fr) 2007-03-08
WO2007027353A3 (fr) 2007-06-07

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