EP2045005A2 - Vorrichtungen und Verfahren zur Durchführung chemischer Reaktionen unter Verwendung von durch Licht erzeugten Reagenzien - Google Patents

Vorrichtungen und Verfahren zur Durchführung chemischer Reaktionen unter Verwendung von durch Licht erzeugten Reagenzien Download PDF

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EP2045005A2
EP2045005A2 EP08168964A EP08168964A EP2045005A2 EP 2045005 A2 EP2045005 A2 EP 2045005A2 EP 08168964 A EP08168964 A EP 08168964A EP 08168964 A EP08168964 A EP 08168964A EP 2045005 A2 EP2045005 A2 EP 2045005A2
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EP
European Patent Office
Prior art keywords
reaction
microfluidic
reactor according
reactor
chamber
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EP08168964A
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English (en)
French (fr)
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EP2045005A3 (de
Inventor
Xiaochuan Zhou
Tiecheng Zhou
David Sun
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Xeotron Corp
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Xeotron Corp
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Withdrawn legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/045—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers using devices to improve synthesis, e.g. reactors, special vessels
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00709—Type of synthesis
    • B01J2219/00711—Light-directed synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
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Definitions

  • the main advantages of the new method include the minimum change to the well-established conventional synthesis procedure, commercial availability and low cost for the chemical reagents involved, and high yield comparable to that achievable with conventional synthesis procedure.
  • This method can be extended to control or initiate other chemical/biochemical reactions by light with the use of various properly chosen photogenerated reagents (PGR), such as photogenerated acids and bases.
  • PGR photogenerated reagents
  • microfluidic reactor embodiments are provided, as well as methods of using and methods of preparing the improved microfluidic reactors.
  • POP photogenerated-acid precursor
  • PGA photogenerated-acid
  • the illumination chamber 103 and the reaction chamber 105 which are part of a reaction cell, are spatially separated so that light exposure hv is prevented from being applied into the reaction chamber 105.
  • the solution 112 spends a sufficient amount time in the connection channel 104 so that any free radicals that may be generated in the illumination chamber 103 would be deactivated before the solution 112 entering the reaction chamber 105.
  • the preferred time for the solution 112 to spend in the connection channel 104 is longer than the half lifetime of the free radicals.
  • the more preferred time for the solution 112 to spend in the connection channel 104 is longer than twice the half lifetime of the free radicals. This would minimize the possibility of undesirable free-radical-induced side-reactions from taking place in the reaction chamber 105 of the reaction cell.
  • the silicon microfluidic template 310 is formed using etching processes which are well know to those skilled in the art of semiconductor processes and microfabrication (Madou, M., Fundamentals of Microfabrication , CRC Press, New York, (1997)).
  • the top surface 313 of the microfluidic template 310 is preferably coated with silicon dioxide, which can be made by either oxidation or evaporation during a fabrication process.
  • silicon dioxide can be made by either oxidation or evaporation during a fabrication process.
  • plastic materials are preferred. Plastic materials may also be preferred for large quantity production of the multi-cell reactor device even when the distance between adjacent reaction cells is less than 5,000 ⁇ m.
  • a feed solution 431 containing photogenerated reagent precursor flows from an inlet duct 422 into an illumination chamber 423.
  • active chemical reagents are produced and the resulted reactive solution 432 flows through a connection channel 424 into a reaction chamber 425.
  • the reactive solution 432 is in contact with immobilized molecules 441 and 442 on the top surface 413 of the microfluidic template 410 and the inner surface 462 of the second window plate 461, respectively.
  • Chemical reactions take place between the active reagents in the reactive solution 432 and the immobilized molecules 441 and 442.
  • the effluent 433 flows through an outlet duct 426 into the outlet channel 427.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Nanotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Physics & Mathematics (AREA)
  • Composite Materials (AREA)
  • Clinical Laboratory Science (AREA)
  • General Physics & Mathematics (AREA)
  • Materials Engineering (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micromachines (AREA)
  • Saccharide Compounds (AREA)
EP08168964A 2000-07-03 2001-07-03 Vorrichtungen und Verfahren zur Durchführung chemischer Reaktionen unter Verwendung von durch Licht erzeugten Reagenzien Withdrawn EP2045005A3 (de)

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US21571900P 2000-07-03 2000-07-03
EP01952397A EP1301268A2 (de) 2000-07-03 2001-07-03 Verfahren und vorrichtungen zur durchführung chemischer reaktionen unter anwendung von photochemisch erzeugten reagenzien

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EP01952397.6 Division 2001-07-03
EP01952397A Division EP1301268A2 (de) 2000-07-03 2001-07-03 Verfahren und vorrichtungen zur durchführung chemischer reaktionen unter anwendung von photochemisch erzeugten reagenzien

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EP2045005A2 true EP2045005A2 (de) 2009-04-08
EP2045005A3 EP2045005A3 (de) 2011-12-21

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EP01952397A Ceased EP1301268A2 (de) 2000-07-03 2001-07-03 Verfahren und vorrichtungen zur durchführung chemischer reaktionen unter anwendung von photochemisch erzeugten reagenzien

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US (5) US20020012616A1 (de)
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