EP2428272A2 - Seringue pour pipette à revêtement hydrophobe - Google Patents

Seringue pour pipette à revêtement hydrophobe Download PDF

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Publication number
EP2428272A2
EP2428272A2 EP11173625A EP11173625A EP2428272A2 EP 2428272 A2 EP2428272 A2 EP 2428272A2 EP 11173625 A EP11173625 A EP 11173625A EP 11173625 A EP11173625 A EP 11173625A EP 2428272 A2 EP2428272 A2 EP 2428272A2
Authority
EP
European Patent Office
Prior art keywords
pipetting
tip
hydrophobic
region
pipette tip
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP11173625A
Other languages
German (de)
English (en)
Other versions
EP2428272A3 (fr
EP2428272B1 (fr
Inventor
Vinzenz Kirste
Renato Nay
Rainer Beckbissinger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hamilton Bonaduz AG
Original Assignee
Hamilton Bonaduz AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hamilton Bonaduz AG filed Critical Hamilton Bonaduz AG
Publication of EP2428272A2 publication Critical patent/EP2428272A2/fr
Publication of EP2428272A3 publication Critical patent/EP2428272A3/fr
Application granted granted Critical
Publication of EP2428272B1 publication Critical patent/EP2428272B1/fr
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Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B01L2300/166—Suprahydrophobic; Ultraphobic; Lotus-effect
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05C—APPARATUS FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05C17/00—Hand tools or apparatus using hand held tools, for applying liquids or other fluent materials to, for spreading applied liquids or other fluent materials on, or for partially removing applied liquids or other fluent materials from, surfaces
    • B05C17/005—Hand tools or apparatus using hand held tools, for applying liquids or other fluent materials to, for spreading applied liquids or other fluent materials on, or for partially removing applied liquids or other fluent materials from, surfaces for discharging material from a reservoir or container located in or on the hand tool through an outlet orifice by pressure without using surface contacting members like pads or brushes
    • B05C17/00503—Details of the outlet element

Definitions

  • the present invention relates to a pipetting tip for aspirating and dispensing pipetting fluid which extends along a pipetting tip longitudinal axis, wherein a first axial longitudinal end region of the pipetting tip has a pipetting longitudinal end region having a pipetting opening operable by pipetting fluid, and a second opposing pipetting longitudinal end region in the axial direction axial longitudinal end portion of the pipette tip as a coupling longitudinal end region coupling geometry for, preferably releasable, coupling to a coupling counter-geometry of a pipetting device, the pipette tip on its outer side an outer hydrophobia area and on its inside an inner hydrophobicity each with a square roughness in a range of 100 nm to 1000 nm, preferably from 150 nm to 750 nm and more preferably from 200 nm to 500 nm, and having a peak-to-peak roughness in a range vo n 800 nm to 5500 nm, preferably from 1750 nm
  • Such pipetting tips are for example from the WO 03/013731 A known.
  • the roughness ranges mentioned serve the hydrophobic formation of surfaces by utilizing the so-called “lotus effect", as it is also observed on lotus flowers.
  • hydrophobic formation of surfaces of pipette tips facilitates the complete emptying of the pipette tip and thus increases the dimensional accuracy dispensed liquid quantities. Furthermore, hydrophobic formation of surfaces of pipetting tips also reduces the risk of undesirable contamination of pipetting fluids in case of multiple use of a pipette tip. This problem is also referred to in the literature as "cross contamination". It results from the fact that a remainder of a first pipetting fluid remains adhering to a surface of the pipette tip as a wetting droplet from a previous pipetting operation and can thus enter a subsequently pipetted second pipetting fluid.
  • the WO 03/013731 A discloses for hydrophobic formation of a pipette tip a method which initially provides for providing a polymer surface at the pipetting tip. This can be done by preparing the pipetting tip from a corresponding polymer or by coating a pipetting tip by immersion in a corresponding polymer melt.
  • the polymer surface is dissolved with a solvent which contains undissolved particles, which after the removal of the solvent are at least partially firmly bonded to the polymer surface.
  • the particles are present in the solvent dispersed or suspended at the beginning of the process.
  • the hydrophobic surface area on the outside of the pipette tip differs in terms of its axial length relative to the pipette tip longitudinal axis from the axial extension region of the hydrophobic region on the inside of the pipette tip.
  • the pipette tip can and therefore only needs to be made hydrophobic in those areas in which such a design is actually required.
  • the inside of the pipetting tip is considered to be the side whose surface has a normal vector which has an extension component to the imaginary pipetting tip longitudinal axis. Accordingly, the outside is the side whose surface has a normal vector which has an extension component away from the pipette tip longitudinal axis.
  • Pipetting tip assists the complete emptying of the pipette tip during dispensing, it is advantageous if the outer hydrophobicity region and the inner hydrophobicity range each extend differently from one edge of the pipetting opening in the axial direction. As a result, it can be ensured that the edge of the pipetting opening through which the pipetting fluid must pass during dispensing receives a hydrophobic formation.
  • the pipetting tip is less, and in some cases even less deeply, immersed in a pipetting fluid reservoir, as pipetting fluid is aspirated high into the pipetting fluid receiving space of the pipetting tip bounded by the inside of the pipetting tip.
  • This can be taken into account by the fact that the end of the inner hydrophobic area axially further from the pipetting opening is located further from the pipetting opening than the end of the outer hydrophobic area axially further from the pipetting opening.
  • pipetting therefore suffices, starting from the edge of the pipetting opening on the outside of the pipetting tip, to provide a hydrophobic formation of the surface only over a smaller axial extension length than on the inside of this pipetting tip, in order to ensure that the surfaces wetted by the pipetting fluid are as intended the pipette tip are hydrophobic.
  • the edge of the pipetting opening is made hydrophobic. Since a drop of pipetting fluid which wets the pipette tip surface extends over a more or less large wetting spot on the pipette tip surface, it is particularly preferred to support a complete emptying of the pipette tip such that the outer hydrophobic area and the inner hydrophobic area form a coherent hydrophobicity region over a border of the pipetting opening defining a boundary between the outside and the inside of the pipetting tip.
  • the hydrophobic embodiment according to the present application is based on the provision of an initially defined roughness of the corresponding surface areas, the desired surface roughness of injection-molded pipetting tips can be produced by corresponding roughness of the mold cavity surfaces generating the surface regions.
  • the pipetting tip may have a more hydrophobic coating in at least one hydrophobic area of inner hydrophobic area and outer hydrophobic area compared to the material of the uncoated pipetting point.
  • the pipetting device includes a pipetting channel in which the negative pressure and / or overpressure is generated and / or provided, which is required for the aspiration and dispensing of pipetting fluid into and out of a pipetting tip.
  • Aerosol contamination is achieved by sucking vaporized or atomized portions of a pipetting fluid aspirated into the pipette tip from the pipetting fluid into the respectively coupled pipetting channel.
  • the vaporized or atomized pipetting liquid can then undesirably pass from the pipetting channel back into the pipetting fluid receiving space of a pipetting tip during a subsequent pipetting operation and contaminate the pipetting fluid received there. This can be done on the basis of the described pollution mechanism involving the pipette tip separate pipetting even with the one-time use of disposable pipetting tips on one and the same pipetting.
  • the filter is preferably provided closer to the coupling longitudinal end portion than to the pipetting longitudinal end portion of the pipetting tip.
  • the filter is preferably made of porous, gas-permeable material, such as a sintered plastic material or fiber tangle, or a combination of such materials.
  • gas-permeable filters substantially more effectively prevent undesired passage of moisture when they are at least partially hydrophobic on their porous surface.
  • this can be achieved particularly advantageously by providing a coating which is more hydrophobic compared to the uncoated material of the filter in at least one section of the filter.
  • the wettability of the filter material and thus also the pore wall of the filter in the coated area drops considerably, which leads to an increase in the wetting angles that can be measured between the filter material and a droplet adhering thereto.
  • the wetting angle increases, one and the same droplets adhering to the filter material project more and more away from it, so that with an increasingly hydrophobic coating of the filter material, a smaller amount of liquid is sufficient to render the filter virtually gas-impermeable by narrowing the flow paths.
  • the applicant reserves the right to seek independent protection regardless of a hydrophobic formation of areas of the pipette tip receiving the filter.
  • Such a filter which is at least partially hydrophobic, can thus also be provided in a pipetting tip which is not designed to be hydrophobic or only on its inside or only on its outside or as described above.
  • the filter is provided in a built-up in the pipette tip preferably at least at that end region with a compared to the material of the uncoated filter more hydrophobic coating, which faces the pipetting.
  • the filter in order to increase the effect of the filter, it is particularly preferred to make the filter completely hydrophobic, in particular to provide it with the abovementioned hydrophobic coating.
  • the pipetting tip described hitherto can preferably be realized in that the uncoated pipetting tip has a plastic material at least on its outside and / or on its inside.
  • the pipetting tip over its entire thickness comprises a uniform plastic material, preferably formed from the plastic material.
  • plastic material a polymer or a copolymer such as polypropylene or / and polyethylene has been found, as well as polyamide. Due to their material properties, these materials are already liquid-repellent on their surface. Likewise, blends of these plastics can be used.
  • the hydrophobic coating has a plastic material, which is preferably compatible with the plastic material of the pipetting tip for easier connection.
  • the pipette tip and the hydrophobic coating on the same plastic material.
  • a pipetting tip which has proved to be hydrophobic in the uncoated state, at least on its side, has proved to be particularly advantageous Coating specific area comprises polypropylene, preferably made of polypropylene, and wherein the hydrophobic coating comprises a polypropylene-polyethylene copolymer.
  • the above-mentioned object is also achieved according to a method aspect of the present invention by a method for hydrophobic coating of pipette tips, which comprises wetting at least portions of the outside and inside of the pipette tip with a wetting solution.
  • the method of the invention comprises a step of coupling the pipetting tip to a fluid pressure source of variable fluid pressure.
  • a fluid pressure is meant which serves for sucking and discharging pipetting fluid, that is to say a pressure of a working fluid other than the pipetting fluid, as a rule of a gas, in particular of air.
  • the inventive method comprises immersing the coupled pipette tip in the wetting solution, whereby it is possible with the simplest means to wet the outside of the pipette tip depending on the depth of immersion.
  • the method according to the invention comprises the step of aspirating wetting solution into the pipetting tip, whereby the inside of the pipetting tip can be wetted by wetting solution and thus provided with a hydrophobic coating.
  • the areas of the pipette tip wetted with wetting solution form the hydrophobically coated areas of the pipette tip after completion of the process.
  • the inventive method comprises dispensing the aspirated wetting solution, so that the pipetting tip can be made free again after wetting.
  • the method of the invention further comprises the step of evaporating solvent contained in the wetting solution whereby the wetted solution wetted portions of the pipetting tip are dried to form a hydrophobic coating.
  • the pipette tip provided with a hydrophobic coating is usually finished coated.
  • the outer hydrophobicity region should extend in the axial direction less far away from the pipetting opening of the pipette tip than the inner hydrophobic region, it can be provided according to a development of the method according to the invention that the height of the aspirated wetting solution column in the pipette tip differs from the immersion depth of the pipette tip in the wetting solution, preferably exceeds the latter.
  • the provision of the receiving cavity may preferably be effected by coupling such a receiving cavity to a pipetting device.
  • a tube element in particular a glass tube element with a smaller diameter than at least the closer the coupling longitudinal end portion of the pipette tip is used as a receiving cavity, so that the receiving cavity can be introduced from a coupling longitudinal end of the pipette forth here.
  • the outer hydrophobicity region can therefore have an axial extension length of zero in this case.
  • the pipetting tip can be connected to the receiving cavity in any desired manner, preferably in such a way that the pipetting tip surrounds the receiving cavity from its coupling-side longitudinal end, so that when the wetting solution is dispensed from the receiving cavity, an interior region of the pipetting tip is rinsed with a binding solution.
  • the pipette tip can be connected directly to the receiving cavity by the pipette tip is attached to the receiving cavity.
  • the pipette tip can be indirectly connected to the receiving cavity via the common pipetting device, so that pipette tip and receiving cavity are connected to the same fluid pressure source.
  • a pipetting device and a reservoir of wetting solution are sufficient.
  • the pipetting device for coupling the receiving cavity must be slightly modified, but this is not absolutely necessary for the use of the present invention.
  • the evaporation of the solvent can advantageously be effected thermally and / or convectively, for example by virtue of the fact that the evaporation step comprises heating the pipetting tip and / or flowing the pipetting tip with a fluid, preferably with a gas, more preferably with air, in particular dry air.
  • the method comprises providing the wetting solution at a temperature in a range of 65 ° C to 85 ° C, preferably in a range of 70 ° C to 80 ° C, more preferably about 75 ° C includes.
  • the wetting solution preferably comprises a polymer or copolymer, particularly preferably a polypropylene-polyethylene copolymer, and a solvent which dissolves the polymer or copolymer contained.
  • a solvent in particular xylene-based solvents have proven to be advantageous.
  • a coating thus obtained leads to a surface roughness in the aforementioned range.
  • the wetting solution has a flow rate of from 0.3 ml / s to 0.7 ml / s, preferably from 0.4 ml / s to 0 , 6 ml / s is dispensed through the pipette tip or / and when the wetting solution at a temperature of 20 ° C to 30 ° C, preferably from 21 ° C to 25 ° C, particularly preferably from 22 ° C to 25 ° C by the pipette tip is dispensed through.
  • FIG. 1 a pipetting tip according to the invention is generally designated 10.
  • the pipetting tip 10 extends along a pipetting tip longitudinal axis L from a pipetting opening 12 to a coupling longitudinal end 14.
  • the pipetting tip 10 comprises a first pipetting longitudinal end region 16 which includes the pipetting opening 12, and further comprises a second coupling longitudinal end region 18 which includes the coupling longitudinal end 14.
  • the coupling longitudinal end region 18 is known to be provided with a coupling inner geometry 20 for releasable form-fitting engagement with a coupling counter-geometry of a pipetting device (see FIG. 2 ).
  • the coupling internal geometry 20 can have a circumferential groove 22 around the pipetting tip longitudinal axis L, into which, in the coupled state of the pipetting tip 10, an elastomer ring squeezed in the axial direction and thus extended in the radial direction (see FIG FIG. 2 ) can engage positively.
  • a pipetting fluid receiving space 24 is advantageously provided into which pipetting fluid aspirates through the pipetting opening 12 and from which pipetting fluid can be dispensed again via the same route.
  • Pipette tip 10 exemplified is preferably injection molded from polypropylene, as this material is less wettable by water than other materials, which, when dispensing pipetting fluid, typically pipetting fluid, desirably facilitates complete emptying of pipette tip 10.
  • a part of the surface of the pipetting tip 10 is made hydrophobic beyond the hydrophobic basic material properties of the preferably used polypropylene.
  • a portion of the pipette tip surface of the inner side 28 of the pipette tip 10 is hydrophobic as the inner hydrophobic region 26, and further, a portion of the surface of the outer surface 30 of the pipette tip 10 is made hydrophobic as the outer hydrophobic region 32.
  • the inner hydrophobicity region 26 and the outer hydrophobicity region 32 coalesce over the edge 34 of the pipetting orifice 12 to form a unitary contiguous hydrophobic surface region of the pipette tip 12.
  • This has the advantage that the edge 34 of the pipetting orifice wetted with pipetting fluid is particularly frequent 12 is hydrophobic, so that the risk of undesired adhesion of Pipettierfluidtröpfchen is at least reduced.
  • the hydrophobic formation of surface areas of the pipette tip 10 is accomplished by providing a defined roughness, such as by providing a surface having a roughness in the range of 220 to 300 nm and with a peak-to-peak roughness in the range of 3000 to 3300 nm.
  • the pipette tip 10 was advantageously immersed first with its outer hydrophobic area 32 in a wetting solution with a polypropylene-polyethylene copolymer dissolved in a xylene-based solvent, so that the entire outer hydrophobic area 32 was wetted by said wetting solution.
  • wetting solution was aspirated into the pipetting fluid receiving space 24 until the surface of the inner side 28 in the region of the inner hydrophobic area 26 was also wetted with wetting solution.
  • the pipetting tip 10 wetted by a residual wetting solution film, was convectively dried in a gas stream after completion of the dispensing process.
  • the wetting solution provided is effectively used, since it is applied only there on the pipette tip 10, where in the later pipetting is actually needed.
  • the axial extent of the inner hydrophobic region 26 is approximately four times the axial extent of the outer hydrophobic region 32. However, this need not be so.
  • the internal hydrophobicity range may also be twice, three times, five times, or a non-integer multiple of the axial extent of the external hydrophobicity region.
  • the pipetting tip 10 preferably at a region closer to the coupling longitudinal end region 18, may have a filter 36 which reduces the risk of aerosol contamination of the distance between the pipetting tip axially located between it and the coupling longitudinal end 14 10 and thus in particular an aerosol contamination of a coupled to the pipette tip 10 pipetting device is used.
  • the filter 36 can be formed, for example, of porous, gas-permeable material in the dry state, for example of sintered plastic, in particular sintered polypropylene and / or polyethylene, and / or of a fiber entanglement.
  • this may, as in FIG. 1 indicated, at least partially, here: about its half axial total extension, also be formed hydrophobic by wetting with the wetting solution described above.
  • the filter 36 is wetted by the hydrophobic coating advantageously worse than in the uncoated state, resulting in that deposited on the filter material pipetting fluid droplets in the deposited state protrude more strongly from the filter material than if the filter material were uncoated, whereby the precipitate on the filter material unwanted Pipettierfluidtröpfchen faster than in the case of an uncoated filter material to close the pores responsible for the gas permeability of the filter material and a Prevent pipetting fluid passage from the pipetting fluid receiving space 24 to the coupling longitudinal end 14 of the pipette tip 10 out advantageous.
  • the filter 36 is thus more appropriate to refer to as a self-regulating moisture-dependent valve, which allows gas to pass in the dry state and to prevent gas passage in the wet state.
  • FIG. 2 a situation is shown which immediately precedes a coating of a portion of the inner surface of a pipetting tip.
  • FIG. 2 The embodiment of the FIG. 2 will be described below only insofar as they differ from that of the FIG. 1 whose description otherwise expressly refers to.
  • the pipette tip 110 of FIG. 2 corresponds exactly in terms of their shape, the pipette tip 10 of FIG. 1 , with the exceptions that the pipette tip 110 has no hydrophobic coating and no filter is provided.
  • the pipetting tip 110 is shown in a state in which it is coupled to a pipetting channel 140.
  • a conical coupling portion 142 corresponding to the conicity of the coupling inner geometry 120 of the pipette tip 110 can be inserted into the pipetting tip 110 in the axial direction from the coupling longitudinal end 114.
  • a relative to the coupling portion 142 axially movable Quetschkolben 144 can be displaced in a conventional manner axially to the coupling portion 142 to thereby squeeze axially between a coupling portion 142 and pinch piston 144 elastomer ring and thereby stretch radially.
  • the elastomeric ring 146 in its crimped state can come into positive engagement with the annular groove 122.
  • a receiving cavity 148 in the form of a glass tube is accommodated on the pipetting channel 140, into which wetting solution 150 has been aspirated through the pipetting channel 40.
  • the pipette tip 110 surrounds the receiving cavity 148 so that it is at least partially received in the pipetting fluid receiving space 124 of the pipetting tip 110.
  • the wetting solution 150 will be dispensed from the receiving cavity 148 by overpressure in the pipetting channel 140 so that it will flush at least one portion of the inner surface 128 of the pipetting tip 110 near the pipetting aperture 112.
  • the wetting solution 150 will also be expelled from the pipetting tip 110 through the pipetting opening 112 so that in the pipetting tip 110 a wetted inner hydrophobing section extending from the edge 134 of the pipetting opening 112 in the axial direction over a certain distance into the pipetting fluid receiving space 124 with a desired roughness, which will be completed after its complete drying.
  • the pipette tip 110 is to be at least partially hydrophobic on its outside 130 at a later time, this can be done by simply dipping the pipetting tip into the corresponding wetting solution and then drying the surface section of the pipetting tip 110 so wetted.
  • filter 36 may be not only partially, but completely hydrophobic, preferably by complete wetting with the corresponding wetting solution.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Application Of Or Painting With Fluid Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
EP11173625.2A 2010-07-12 2011-07-12 Procédé de revêtement hyrophobe d'embouts de pipette Active EP2428272B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102010031240A DE102010031240A1 (de) 2010-07-12 2010-07-12 Pipettierspitze mit hydrophober Oberflächenausbildung

Publications (3)

Publication Number Publication Date
EP2428272A2 true EP2428272A2 (fr) 2012-03-14
EP2428272A3 EP2428272A3 (fr) 2015-04-29
EP2428272B1 EP2428272B1 (fr) 2017-08-23

Family

ID=45372655

Family Applications (1)

Application Number Title Priority Date Filing Date
EP11173625.2A Active EP2428272B1 (fr) 2010-07-12 2011-07-12 Procédé de revêtement hyrophobe d'embouts de pipette

Country Status (5)

Country Link
US (2) US8753715B2 (fr)
EP (1) EP2428272B1 (fr)
JP (2) JP2012073227A (fr)
DE (1) DE102010031240A1 (fr)
NO (1) NO2428272T3 (fr)

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JP6004149B1 (ja) 2015-03-16 2016-10-05 パナソニック株式会社 ピペットチップ及びピペッティング方法
JP6174182B1 (ja) * 2016-03-22 2017-08-02 キヤノンマシナリー株式会社 分注ノズルおよび分注ノズルの製造方法
AU2018313965A1 (en) * 2017-08-11 2020-02-06 Neomed, Inc. Fluid transfer connector
FR3077995B1 (fr) 2018-02-20 2020-02-14 Gilson Sas Procede de fluoration d'un filtre d'embout de pipette, embout de pipette, son procede de fabrication et pipette associes
WO2020050234A1 (fr) * 2018-09-03 2020-03-12 京セラ株式会社 Capillaire et pipette
CN112827533B (zh) * 2021-01-08 2022-03-08 青岛大学 一种基于超疏水材料的桌面型微液滴化学反应实验平台
CN115256781B (zh) * 2022-07-14 2024-10-22 浙江曼薇尼生物科技有限公司 低吸附塑料移液吸头的表面处理方法
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EP2428272A3 (fr) 2015-04-29
US8753715B2 (en) 2014-06-17
JP6140245B2 (ja) 2017-05-31
DE102010031240A1 (de) 2012-01-12
US20120009100A1 (en) 2012-01-12
JP2012073227A (ja) 2012-04-12
EP2428272B1 (fr) 2017-08-23
US8840957B2 (en) 2014-09-23
NO2428272T3 (fr) 2018-01-20
US20130280423A1 (en) 2013-10-24

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