EP2532426A2 - Cartouche, centrifugeuse et procédé - Google Patents

Cartouche, centrifugeuse et procédé Download PDF

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Publication number
EP2532426A2
EP2532426A2 EP20120163519 EP12163519A EP2532426A2 EP 2532426 A2 EP2532426 A2 EP 2532426A2 EP 20120163519 EP20120163519 EP 20120163519 EP 12163519 A EP12163519 A EP 12163519A EP 2532426 A2 EP2532426 A2 EP 2532426A2
Authority
EP
European Patent Office
Prior art keywords
drum
actuator
chamber
cartridge
central axis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP20120163519
Other languages
German (de)
English (en)
Other versions
EP2532426A3 (fr
EP2532426B1 (fr
Inventor
Martina Daub
Juergen Steigert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch GmbH filed Critical Robert Bosch GmbH
Publication of EP2532426A2 publication Critical patent/EP2532426A2/fr
Publication of EP2532426A3 publication Critical patent/EP2532426A3/fr
Application granted granted Critical
Publication of EP2532426B1 publication Critical patent/EP2532426B1/fr
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/15—Use of centrifuges for mixing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/30—Mixing the contents of individual packages or containers, e.g. by rotating tins or bottles
    • B01F29/32—Containers specially adapted for coupling to rotating frames or the like; Coupling means therefor
    • B01F29/321—Containers specially adapted for coupling to rotating frames or the like; Coupling means therefor of test-tubes or the like
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/42—Mixers with shaking, oscillating, or vibrating mechanisms with pendulum stirrers, i.e. with stirrers suspended so as to oscillate about fixed points or axes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
    • B01F33/453—Magnetic mixers; Mixers with magnetically driven stirrers using supported or suspended stirring elements
    • B01F33/4533—Magnetic mixers; Mixers with magnetically driven stirrers using supported or suspended stirring elements supporting the stirring element in one point
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/713—Feed mechanisms comprising breaking packages or parts thereof, e.g. piercing or opening sealing elements between compartments or cartridges
    • B01F35/7137—Piercing, perforating or melting membranes or closures which seal the compartments
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/716—Feed mechanisms characterised by the relative arrangement of the containers for feeding or mixing the components
    • B01F35/7161—Feed mechanisms characterised by the relative arrangement of the containers for feeding or mixing the components the containers being connected coaxially before contacting the contents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
    • B01F35/71725—Feed mechanisms characterised by the means for feeding the components to the mixer using centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0672—Integrated piercing tool
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0841—Drums
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces

Definitions

  • biochemical processes are based in particular on the handling of liquids. Typically, this manipulation is done manually with tools such as pipettes, reaction vessels, active probe surfaces or laboratory equipment. By pipetting robots or special equipment, these processes are already partially automated.
  • Lab-on-a-Chip systems (also referred to as the West Pocket Laboratory or the Chiplabor) accommodate all the functionality of a macroscopic laboratory on a plastic plastic-sized plastic substrate.
  • Lab-on-a-chip systems typically consist of two major components.
  • a test carrier includes structures and mechanisms for the implementation of basic fluidic operations (e.g., mixers), which may consist of passive components such as channels, reaction chambers and upstream reagents, or even active components such as valves or pumps.
  • the second main component is actuation, detection and control units.
  • Such systems make it possible to carry out biochemical processes fully automatically.
  • a lab-on-a-chip system is for example in the document DE 10 2006 003 532 A1 described.
  • This system comprises a rotor chip, which is rotatably provided with respect to a stator chip.
  • the rotor chip can be coupled by means of fluidic channels with the stator chip for filling or emptying the rotor chip.
  • the cartridge defined in claim 1, the centrifuge defined in claim 13 and the method defined in claim 15 have the advantage over conventional solutions that the processing of at least one component in the cartridge can be carried out independently of the rotational speed of the centrifuge.
  • the first and second chambers or the first and third chambers can be conductively connected to one another by actuation of the actuator.
  • the operation can still be supported by the centrifugal force.
  • the first chamber is conductively connected to the second chamber, and the first component is thereafter transferred from the second chamber to the first chamber due to the action of the centrifugal force. Thereafter, the first chamber is in turn connected by actuation of the actuator with the third chamber, and the second component thereafter also flows into the first chamber, for example in order to be mixed with the first component.
  • the first chamber is first conductively connected to the second chamber and filled a certain proportion of the component in the second chamber. Thereafter, the first chamber is conductively connected to the third chamber and filled a further portion of the component in the third chamber.
  • the first and third chambers or the second and third chambers can be conductively connected to one another by actuation of the actuator.
  • the operation can still be supported by the centrifugal force.
  • the second variant at least one first component in the first chamber and a second component in the second chamber held.
  • the first chamber is conductively connected to the third chamber, and the first component is thereafter transferred from the first chamber to the third chamber due to the action of the centrifugal force.
  • the second chamber is in turn connected by actuation of the actuator with the third chamber, and the second component thereafter flows into the third chamber, for example, to be mixed with the first component.
  • the first chamber is first conductively connected to the third chamber and filled a certain proportion of the component in the first chamber.
  • the second chamber is conductively connected to the third chamber and filled a further portion of the component in the second chamber.
  • the first and second variants can also be combined.
  • Component in this case means a liquid, a gas or a particle.
  • chamber is presently preferably a line section, which is formed on both sides or only open on one side, as well as a substantially closed space meant, which has an inlet and / or outlet.
  • first chamber with either the second or with the third chamber (first variant) or either the first chamber with the third chamber or the second chamber with the third chamber (second variant) in dependence is connected by an actuation by the actuator.
  • the actuator is provided electrically, mechanically and / or pressure-operated.
  • a piezoelectrically, electrostatically, semi-mechanically manually or electromagnetically operated actuator is suitable.
  • Printing companies means that the actuator operates the first drum by utilizing a gas or liquid pressure.
  • the actuator has an actuator which is connected to the first drum in order to rotate it directly and if necessary to move along the central axis.
  • the actuator has an actuator, which is connected to the first drum to move along the central axis and thereby rotate.
  • the actuator actuates the ballpoint pen mechanism described below, whereupon the ballpoint pen mechanism rotates the first drum.
  • the first drum is thus indirectly operated.
  • the adjusting device comprises a first bevel, which cooperates with a second bevel of the first drum, in order from a first position in which this is a form-locking engagement with a housing of the cartridge in the rotational direction about the central axis in a second position along the central axis to spend, in which the positive connection is canceled and the first drum rotates about the central axis due to the action of a return means or the actuator or another actuator.
  • a ballpoint pen mechanism is provided.
  • the actuator actuates the first slope for cooperation with the second slope.
  • the actuator moves the first slope in a direction away from the pivot point of the cartridge of the centrifuge along the central axis.
  • the first drum is a second drum upstream or downstream relative to the central axis, wherein the actuator actuates the second drum for rotating the first drum.
  • the first drum is thus actuated indirectly by means of the second drum.
  • the actuator abuts flat against the first and / or second drum for pressing against it, or the actuator is fixedly connected to the first or second drum.
  • the configuration according to which the actuator abuts flat against the first and / or second drum for pressing against it, is characterized by a simple construction and a simple assembly.
  • the fixed connection between the first or second drum and the actuator has the advantage that the actuator can move the first or second drum in opposite directions.
  • the actuator is arranged between the first and second drum.
  • the actuator is preferably designed as a piezoelectric element.
  • the cartridge has a housing which is closed at one end by means of an adapter, wherein the actuator is attached to the adapter.
  • the actuator is integrated in the adapter.
  • the adapter has a flexible membrane which can be actuated on its one side by means of the actuator and acts on its other side on the first drum or the second drum. This can create a more sterile outcome.
  • the actuator is thus preferably outside the interior of the housing.
  • the second and / or third chamber of the first drum upstream or downstream relative to the central axis and / or is formed in the second drum.
  • a second chamber of the first drum and a third chamber of the first drum may be downstream.
  • the second chamber is provided in the second drum and the third chamber is provided in a third drum.
  • the actuator is arranged in front of or behind the first drum with respect to a pivot point of the cartridge in the centrifuge. If the actuator is arranged behind the first drum with respect to the fulcrum, it is advantageous that no other return means is required or this is formed by the actuator itself.
  • the actuation of the first drum is effected by the actuator in cooperation with the centrifugal force.
  • FIG. 1 shows in a sectional view a cartridge 100 according to an embodiment of the present invention.
  • the cartridge 100 includes a housing 102 in the form of a tube.
  • the housing 102 may be formed as a 15 mL centrifuge tube, 1.5 mL or 2 mL Eppendorf tube, or alternatively as a microtiter plate (e.g., 20 ⁇ L per well).
  • the longitudinal axis of the housing 102 is designated 104.
  • a first drum 108, a second drum 106 and a third drum 110 are accommodated in the housing 102.
  • the drums 106, 108, 110 are arranged one behind the other and with their respective central axes coaxial with the longitudinal axis 104.
  • the housing 102 is formed closed at its one end 112. Between the closed end 112 and the drum 110 arranged adjacent thereto, a return means, for example in the form of a spring 114, is arranged.
  • the spring 114 may be in the form of a coil spring or a polymer, in particular an elastomer.
  • the other end 116 of the housing 102 is closed by means of a closure 118.
  • the closure 118 may be removed to remove the drums 106, 108, 110 from the housing 102.
  • the housing 102 itself can be dismantled to remove the drums 106, 108, 110 or to reach the chambers, for example the chamber 136.
  • the spring 114 is disposed between the shutter 118 and the drum 106 so that the spring 114 is stretched to generate a restoring force.
  • Other arrangements of the spring 114 are conceivable.
  • a respective drum 106, 108, 110 may have one or more chambers:
  • the second drum 106 includes a plurality of reagent chambers 120 and another chamber 122 for receiving a sample, such as a blood sample, taken from a patient.
  • a sample such as a blood sample
  • the first drum 108 connected downstream of the second drum 106 comprises a mixing chamber 124, in which the reagents from the chambers 120 with the sample the chamber 122 are mixed.
  • the first drum 108 includes, for example, a chamber 126 in which the mixture from the mixing chamber 124 is separated into a liquid and a solid phase 128 and 130, respectively.
  • the solid phase 130 may be a gel column, a silica matrix, or a filter.
  • the third drum 110 which is in turn connected downstream of the first drum 108, comprises a chamber 132 for receiving a waste product 134 from the chamber 126. Furthermore, the third drum 110 comprises a further chamber 136 for receiving the desired end product 138.
  • the cartridge 100 has an outer geometry so that it can be used in a receptacle of a rotor of a centrifuge, in particular in a receptacle of a swing-bucket rotor or fixed-angle rotor of a centrifuge.
  • the cartridge 100 is moved by one in FIG. 1 schematically indicated pivot point 140 rotated at high speed.
  • the pivot point 140 lies on the longitudinal axis 104, so that a corresponding centrifugal force 142 along the longitudinal axis 104 acts on each component of the cartridge 100.
  • the mixing chamber 124 is first to be fluidly connected to the chamber 122 to receive the sample from the chamber 122. Thereafter, the mixing chamber 124 is to be connected to the chambers 120 to receive the reagents from these. Subsequently, the reagents and the sample are to be mixed in the mixing chamber 124. Similarly, the processes in chambers 126, 132 and 136 should also be controlled. In addition to the control of the processes by means of the actuator 139, the rotational speed of the centrifuge or a rotor thereof can be controlled accordingly.
  • the acting centrifugal force 142 has, in particular, the task of transferring the components, for example the sample or the reagents, between the chambers 120, 122 and the mixing chamber 124.
  • FIG. 2A-2G perspective view of various components of the cartridge 100 from FIG. 1 , Based on Figures 2A-2G in particular an adjusting device 300 (see Fig. 3A ) comprising the actuator 139, which enables the control of the above-mentioned processes.
  • the housing 102 on its inside projections 200.
  • the projections 200 are radially from the housing inner wall 202 toward the longitudinal axis 104.
  • the projections 200 form between them slots 204 which extend along the longitudinal axis 104.
  • the projections 200 are formed at their one end in each case with a slope 206.
  • the slopes 206 face away from the pivot point 140 during operation of the centrifuge with the cartridge 100.
  • FIG. 2B shows the end 112 of the housing 102, which is formed according to this embodiment as a removable cap.
  • the end 112 has at its inner periphery a plurality of grooves 208 which extend along the longitudinal axis 104.
  • FIG. 2C shows the second drum 106 with the chambers 120, 122.
  • the drum 106 has on its outer wall 210 a plurality of projections 212 which extend from the outer wall 210 radially outwardly.
  • the projections 212 of the drum 106 engage in the slots 204 of the housing 102.
  • a rotation of the drum 106 is locked about the longitudinal axis 104.
  • the drum 106 is slidable along the longitudinal axis 104 in the slots 204.
  • the second drum 106 furthermore has on its outer wall 210, in particular on its end 214 facing the first drum 108, a crown-like contour 216 which comprises a multiplicity of bevels 218, 220.
  • Two bevels 218, 220 each form a point of the crown-like contour 216.
  • the ramps 218, 220 also face away from the pivot point 140 during operation of the centrifuge with the cartridge 100.
  • FIG. 2D shows a view of the second drum 106 from Figure 2C from underneath.
  • the underside 222 of the drum 106 assigned to the end 214 has a plurality of openings 224 in order to connect the chambers 120, 122 to the mixing chamber 124 of the first drum 108 in liquid, gas and / or particle form (hereinafter "conductive").
  • the openings 224 may also conductively connect the chambers 120, 122 to the chamber 126 of the first drum 108.
  • a respective conductive connection is determined by the position of a respective opening 224 with respect to the chambers 124, 126. This position is achieved by rotating the first drum 108 relative to the second drum 106, as will be explained in more detail later.
  • FIG. 2E shows a lancing device 226, which in FIG. 1 not shown.
  • Lancing device 226 includes a plate 228 having one or more spikes 230 disposed adjacent to an opening 232 in plate 228, respectively.
  • the mandrels 230 serve to pierce a respective opening 224 in the underside 222 of the second drum 106 by means of suitable control by the actuator 139, whereupon in particular liquid from the corresponding chamber 120, 122 flows through the opening 232 into the chambers 124 or 126 ,
  • Figure 2F shows the first drum 108 with the chambers 124, 126.
  • the first drum 108 has a plurality of projections 240 on its outer wall 238.
  • the protrusions 240 are configured to engage the slots 204 (as well as the protrusions 212 of the second drum 106). As long as the projections 240 are engaged with the slots 240, rotation of the first drum 108 about the longitudinal axis 104 is disabled. However, the projections 240 together with the drum 108 are movable along the longitudinal axis 104 in the slots 204.
  • the projections 240 have bevels 242, which point in the direction of the pivot point 140 during operation of the centrifuge with the cartridge 100 and are formed corresponding to the bevels 206 and 220.
  • FIG. 2G shows the third drum 110 with the chambers 132, 136.
  • the drum 110 has projections 244 which project from the outer wall 246 of the drum 110 respectively.
  • the projections 244 are adapted to engage the grooves 208 of the end 112 so that the drum 110 is displaceable in the longitudinal direction 104 in the grooves 208. A rotation of the drum 110 about the longitudinal axis 104 is thus locked.
  • FIG. 3A-3E show several operating conditions during operation of the cartridge 100 FIG. 1 , wherein an additional drum 302 is shown, but this is not relevant in the present case.
  • the Figures 4A-4E correspond respectively with the Figures 3A-3E and illustrate the movement of the ramps 206, 218, 220, 242 relative to each other.
  • FIG. 3B shows an operating state of the cartridge 100 which is more advanced than that in FIG FIG. 4B shown condition.
  • the housing 102 is shown partially transparent to reveal the interior.
  • FIGS. 3A and 4A show a first position in which the projections 240 of the first drum 108 engage in the slots 204 and thus a rotation of the first drum 108 is locked about the longitudinal axis 104.
  • the actuator 139 now presses indirectly or directly on the second drum 106, the second drum 106 in turn pushes the bevels 242 of the first drum 108 against the action of the spring 114 by means of the bevels 220 of the contour 216, the spring 114 being compressed.
  • the first drum 108 moves in a direction away from the pivot point 140 as indicated by the corresponding arrows in FIGS FIGS. 4A and 4B indicated. This movement is continued until the protrusions 240 disengage from the protrusions 200.
  • the spring 114 again pushes the first drum 108 in the direction of the pivot point 140 by means of the third drum 110.
  • the second drum 106 together with its bevels 220 is likewise moved again in the direction of the pivot point 140 whereby the chamfers 242 of the first drum 108 come to rest against the chamfers 206 of the housing 102 and slide therealong into a third position, as in Figs Figures 4D and 4E shown.
  • the projections 240 of the first drum 108 are again located in the slots 204 of the housing 102, so that further rotation of the first drum 108 about the longitudinal axis 104 is again locked.
  • the process described above may be repeated as many times as desired to rotate the first drum 108 in a defined manner relative to the other drums 106 and 110.
  • the actuator 139 may be electrically, mechanically and / or pressure-operated.
  • a piezoelectrically, electrostatically, semi-mechanically manually or electromagnetically operated actuator 139 is suitable.
  • “Operated” here means the active principle which the actuator 139 exploits in order to generate the actuating force for actuating the first drum 106 (or, depending on the embodiment, also one of the other drums 108, 110).
  • the actuator 139 may include an electromagnet that cooperates with a metal part disposed in one of the drums 106, 108, 110 that the electromagnet attracts or repels by appropriately driving it to thereby adjust the above-described adjustment of the drums 106, 108, 110 to each other to reach.
  • the pressure force applied to the second drum 106 by means of the actuator 139 is typically 0.5-100 N.
  • the compressive force to be applied by the actuator is reduced in accordance with the centrifugal force acting.
  • a suitable, not shown, control device which controls the actuator 139, so that the drums 106, 108, 110 at the desired time to take the respective desired position to each other.
  • the control device may have a timer and / or an integrated circuit.
  • FIG. 5 shows again in a section, but schematically the cartridge 100 from FIG. 1 ,
  • Actuator 139 is preferably formed as a piezoelectric element, which is arranged between the lid 118, which faces the pivot point 140, and the second drum 106.
  • the actuator 139 is on the one hand attached to the lid 139 and pressed on the other hand with its end face 500 against the surface of the drum 106 without being connected to this.
  • the face 500 is shown spaced apart from the drum 106 for clarity only. In fact, however, the return means 114 constantly urges the drum 106 against the actuator 139.
  • the cartridge 100 is rotated around the fulcrum 140 at a constant speed.
  • the speed is chosen such that there is no automatic rotation of the first drum 108 with respect to the other drums 106, 110, ie the ballpoint pen mechanism is not already triggered due to the centrifugal force 142.
  • the actuator 139 is controlled by a corresponding control signal from the controller for applying a force to the second drum 106.
  • the first drum 108 is rotated in such a way that the chambers 120, 124 are opposite one another and conductively connected to one another.
  • the chambers 120, 124 closing cover (not shown) by means of mandrels 230 in this process pierced.
  • a first component 502 flows from the chamber 120 into the mixing chamber 124 under the action of centrifugal force 142.
  • the ball-point pen mechanism is actuated again by means of the actuator 139, whereupon the first drum 108 rotates such that the mixing chamber 124 faces the chamber 122 and with this is conductively connected.
  • a cover film closing the chamber 122 as described, which is pierced in this process, may also be provided.
  • a second component 504 flows from the chamber 122 into the mixing chamber 124 under the action of the centrifugal force 142 and can be mixed there.
  • the movements of the respective components 139, 106, 108, 110 is indicated by arrows.
  • the rotation of the first drum 108 and thus the processing of the components 502, 504 can thus take place largely independently of the centrifugal force 142.
  • FIG. 6 shows a variant opposite FIG. 5 .
  • an actuator 600 is provided which is fixed on the end face 500 of the actuator on the one hand and on the third drum 110 on the other hand.
  • the actuator 600 extends through a hole 602 in the second drum 106 and is connected to the first drum 108 and / or the third drum 110.
  • the restoring force is generated by the actuator 600, so that the spring 114 omitted, see FIG. 5 , can.
  • contraction of the actuator 139 the actuator 600 and thus the drum 108 is pulled upwards.
  • the rotation of the first drum 108 is performed as in the embodiment Fig. 5 ,
  • FIG. 7 shows another variant opposite FIG. 5 .
  • the actuator 139 is disposed between the fulcrum 140 and the second drum 106 and the first drum 108, respectively, in the embodiment according to FIG Fig. 7 the actuator 139 is disposed between the end 112 of the housing 102 and the third drum 110.
  • the actuation and return of the ballpoint pen mechanism is carried out in this embodiment by means of the actuator 139 or alternatively in conjunction with the centrifugal force 142nd
  • a spring 800 may additionally be provided between the lid 118 of the second drum 106, which biases the drums 106, 108, 110 against the actuator 139. This is in Fig. 8 shown, which is a variant opposite FIG. 7 represents.
  • Fig. 9 shows a still further variant opposite Fig. 5 .
  • FIG. 9 only the first drum 108 and the second drum 106 are provided.
  • An actuator 600 in the form of a shaft is connected on the one hand to the actuator 139 and on the other hand to the first drum 108.
  • the shaft 600 passes through a hole in the second drum 106, which provided fixed according to this embodiment.
  • the actuator 139 in particular an electric motor rotates the shaft 600 and thereby the first drum 108 about the central axis 104, whereby different chambers 120, 122, 124 are conductively connected to each other, as described above.
  • a ballpoint pen mechanism is not provided in this embodiment.
  • the actuator 139 may be further configured to also move the shaft 139 along the centerline 104 to thereby space the first drum 108 from the second drum 106 for rotation and to press the drums 106, 108 together again after rotation. whereby a tight, conductive connection is provided, for example, between the chamber 120 and the chamber 124.
  • the second drum 106 may be movably provided according to another embodiment by means of another actuator, not shown.
  • Fig. 10 shows a still further variant opposite Fig. 5 .
  • the lid 118 is designed in the form of an adapter for holding the actuator 139.
  • the actuator 139 extends through the adapter 118 and thus acts directly on the second drum 106 to this in a direction away from the pivot point 140, ie in Fig. 11 down, to move.
  • the actuator 139 for this purpose, an actuator, in particular a rod having, which presses against the drum 106.
  • the provision can be made as described above by means of the return means 114.
  • the actuator 139 such as the actuator, is fixedly connected to the second drum 106. This would allow the drum 106 to be rapidly reciprocated along the longitudinal direction 104 by means of the actuator 139, whereby a mixing chamber for mixing components in one of the chambers 120, 122 could be provided. If the amplitude of the to-and-fro motion is chosen to be sufficiently small, this movement can occur without twisting the drums 106, 108, 110 relative to each other, i. without triggering the ballpoint pen mechanism.
  • the embodiment according to FIG. 11 differs from the according to FIG. 10 in that the actuator 139 is attached to the outside of the adapter 118, ie, the actuator 139 does not penetrate the adapter 118 in this case. Rather, the actuator 139 acts indirectly, for example by means of a flexible membrane, on the second drum 106 to operate them in the first direction 207.
  • a section 1100 of the adapter 118 is made thin, wherein an actuated by the actuator actuator 139 600 deforms this thin portion 100 elastically.
  • the actuator 139 pressure-operated, for which the actuator is pressure-conductively connected to a pressure device, not shown, for example, a gas pressure cylinder, and is driven by this.
  • a pressure device not shown, for example, a gas pressure cylinder
  • the adapter 118 and the second drum 106 form a chamber with each other, which is acted upon by pressure from the pressure device and thus the actuator 139 is formed.
  • the actuator 139 could be provided in the form of a bellows, which is provided between the adapter 118 and the second drum 106.
  • the actuator 139 can also be provided elsewhere, for example between the first drum 108 and the second or third drum 106, 110.
  • the actuator 139 is preferably designed as a piezoelectric element.
  • Fig. 12 shows in a plan view schematically a centrifuge 1200 according to an embodiment of the present invention.
  • the centrifuge 1200 includes a rotor, not shown.
  • the cartridge 100 is inserted into the rotor and is moved on a circular path 1202.
  • a coil 1204 is provided, which drives the actuator 139 as soon as it passes through the coil 1204.
  • the actuator 139 then triggers the above-described rotational movement of the first drum 108.
  • a transmitting device not shown, could be provided which generates electromagnetic waves, in particular radio waves, short range.
  • An unillustrated, integrated into the cartridge 100 receiver device then controls the actuator 139, as soon as it passes the transmitter.
  • the actuator 139 can therefore be controlled wirelessly by a stationary control device, for example provided in the centrifuge housing. But also a control by means of sliding contact is conceivable.
  • the cartridge 100 could include a computer unit, not shown, which controls the actuator 139 on the basis of a computer program.
  • a start time of the computer program can be set by a user or transmitted wirelessly to the computer device by means of the aforementioned control device.
  • a power supply, not shown, for the actuator 139 can be integrated in the cartridge 100, for example in the form of a battery in the housing 102.
  • Electrical energy can be supplied externally wirelessly or by wire.
  • the energy can be induced by means of one or more coils (wireless).
  • the energy can be supplied via a sliding contact (wired).
  • a control unit controls the interaction of the actuator 139, which determines the spatial positioning of the drums 106, 108, 110, and the rotational speed of the centrifuge 1200 and the rotor, which in particular controls the flow rate of the components 502, 504 through the cartridge 100.
  • the second drum 106 and / or the third drum 110 may be fixed or movable with respect to the housing 102.
  • the drums 106, 110 may be provided, for example, each rotatable about the central axis 104 by means of a further actuator.
  • the mixing chamber 124 may comprise an obstacle structure, not shown, for example a sieve or a grid structure which is adapted to move through the liquids 502, 504 under the action of a centrifugal force (ie when the rotational speed of the centrifuge exceeds a predetermined threshold). in order to mix these.
  • an obstacle structure not shown, for example a sieve or a grid structure which is adapted to move through the liquids 502, 504 under the action of a centrifugal force (ie when the rotational speed of the centrifuge exceeds a predetermined threshold). in order to mix these.
  • the housing 102 and the drums 106, 108, 110 may be made of the same or different polymers.
  • the one or more polymers are, in particular, thermoplastics, elastomers or thermoplastic elastomers. Examples are cycloolefin polymer (COP), cycloolefin copolymer (COC), polycarbonates (PC), polyamides (PA), polyurethanes (PU), polypropylene (PP), polyethylene terephthalate (PET) or poly (methyl methacrylate) ( PMMA).
  • COP cycloolefin polymer
  • COC cycloolefin copolymer
  • PC polycarbonates
  • PA polyamides
  • PU polyurethanes
  • PP polypropylene
  • PET polyethylene terephthalate
  • PMMA poly (methyl methacrylate)
  • the second drum 106 and / or the third drum 110 may be formed integrally with the housing 102.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Centrifugal Separators (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
EP12163519.7A 2011-06-07 2012-04-10 Cartouche destinée à être inséré dans une centrifugeuse, centrifugeuse et procédé Not-in-force EP2532426B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102011077124A DE102011077124A1 (de) 2011-06-07 2011-06-07 Kartusche, Zentrifuge sowie Verfahren

Publications (3)

Publication Number Publication Date
EP2532426A2 true EP2532426A2 (fr) 2012-12-12
EP2532426A3 EP2532426A3 (fr) 2013-03-27
EP2532426B1 EP2532426B1 (fr) 2015-11-18

Family

ID=46027604

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12163519.7A Not-in-force EP2532426B1 (fr) 2011-06-07 2012-04-10 Cartouche destinée à être inséré dans une centrifugeuse, centrifugeuse et procédé

Country Status (4)

Country Link
US (1) US9272278B2 (fr)
EP (1) EP2532426B1 (fr)
CN (1) CN102814242A (fr)
DE (1) DE102011077124A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2532427A3 (fr) * 2011-06-07 2013-03-27 Robert Bosch Gmbh Cartouche, centrifugeuse et procédé
EP2532428A3 (fr) * 2011-06-07 2013-04-03 Robert Bosch Gmbh Cartouche, centrifugeuse ainsi que procédé de mélange d'un premier et d'un deuxième composant
EP2754494A1 (fr) * 2013-01-14 2014-07-16 Robert Bosch Gmbh Cartouche, centrifuge pour cette cartouche et procédé

Families Citing this family (7)

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US9308508B2 (en) * 2013-07-22 2016-04-12 Kianoosh Peyvan Sequential delivery device and method
US10112199B2 (en) 2014-12-03 2018-10-30 Fiberlite Centrifuge, Llc Centrifuge sample container and closure therefore
US9987634B2 (en) 2014-12-03 2018-06-05 Fiberlite Centrifuge, Llc Centrifuge sample container and closure therefor
USD777941S1 (en) * 2015-07-17 2017-01-31 Fiberlite Centrifuge, Llc Centrifuge bottle
DE102017115191A1 (de) * 2017-07-06 2019-01-10 A. u. K. Müller GmbH & Co. KG Ventil, insbesondere Servoventil
KR102028953B1 (ko) * 2019-02-01 2019-10-07 비아로지스 주식회사 생체 원료 제조 장치 및 그의 구동 방법
DE102023208590A1 (de) 2023-09-06 2025-03-06 Robert Bosch Gesellschaft mit beschränkter Haftung Freisetzvorrichtung für ein Analysegerät zum Analysieren einer in einer Kartusche enthaltene Probe, Analysegerät und Verfahren zum Betreiben des Analysegerätes

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DE102006003532A1 (de) 2006-01-24 2007-07-26 INSTITUT FüR MIKROTECHNIK MAINZ GMBH Mikrofluidische Anordnung und modulares Lab-On-A-Chip-System

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Cited By (5)

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Publication number Priority date Publication date Assignee Title
EP2532427A3 (fr) * 2011-06-07 2013-03-27 Robert Bosch Gmbh Cartouche, centrifugeuse et procédé
EP2532428A3 (fr) * 2011-06-07 2013-04-03 Robert Bosch Gmbh Cartouche, centrifugeuse ainsi que procédé de mélange d'un premier et d'un deuxième composant
US9399214B2 (en) 2011-06-07 2016-07-26 Robert Bosch Gmbh Cartridge, centrifuge and method
US9475043B2 (en) 2011-06-07 2016-10-25 Robert Bosch Gmbh Cartridge, centrifuge and method for mixing a first and second component
EP2754494A1 (fr) * 2013-01-14 2014-07-16 Robert Bosch Gmbh Cartouche, centrifuge pour cette cartouche et procédé

Also Published As

Publication number Publication date
US9272278B2 (en) 2016-03-01
EP2532426A3 (fr) 2013-03-27
EP2532426B1 (fr) 2015-11-18
US20120316050A1 (en) 2012-12-13
CN102814242A (zh) 2012-12-12
DE102011077124A1 (de) 2012-12-13

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