EP2910258A2 - Appareil de concentration de plasma riche en plaquettes - Google Patents

Appareil de concentration de plasma riche en plaquettes Download PDF

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Publication number
EP2910258A2
EP2910258A2 EP15159104.7A EP15159104A EP2910258A2 EP 2910258 A2 EP2910258 A2 EP 2910258A2 EP 15159104 A EP15159104 A EP 15159104A EP 2910258 A2 EP2910258 A2 EP 2910258A2
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EP
European Patent Office
Prior art keywords
prp
assembly
concentrator
separation
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP15159104.7A
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German (de)
English (en)
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EP2910258A3 (fr
EP2910258B1 (fr
Inventor
Randel Dorian
Michael Leach
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Hanuman LLC
Biomet Biologics LLC
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Hanuman LLC
Biomet Biologics LLC
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Publication of EP2910258A2 publication Critical patent/EP2910258A2/fr
Publication of EP2910258A3 publication Critical patent/EP2910258A3/fr
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Publication of EP2910258B1 publication Critical patent/EP2910258B1/fr
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/02—Blood transfusion apparatus
    • A61M1/0281—Apparatus for treatment of blood or blood constituents prior to transfusion, e.g. washing, filtering or thawing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/34—Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration
    • A61M1/3472—Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration with treatment of the filtrate
    • A61M1/3482—Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration with treatment of the filtrate by filtrating the filtrate using another cross-flow filter, e.g. a membrane filter
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/34—Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration
    • A61M1/3496—Plasmapheresis; Leucopheresis; Lymphopheresis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3621—Extra-corporeal blood circuits
    • A61M1/3627—Degassing devices; Buffer reservoirs; Drip chambers; Blood filters
    • A61M1/3633—Blood component filters, e.g. leukocyte filters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3679—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits by absorption
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
    • A61M1/3696—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P41/00—Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2202/00—Special media to be introduced, removed or treated
    • A61M2202/04—Liquids
    • A61M2202/0413—Blood
    • A61M2202/0415—Plasma
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2202/00—Special media to be introduced, removed or treated
    • A61M2202/04—Liquids
    • A61M2202/0413—Blood
    • A61M2202/0427—Platelets; Thrombocytes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/44—Cartridge types
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/58—Parts of membrane modules specifically adapted for single use
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B04B2005/0478—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation with filters in the separation chamber

Definitions

  • This invention relates to a device and method for preparing platelet-plasma concentrates with improved wound healing properties for use as a tissue sealant and adhesive.
  • the product has a fully active (un-denatured) fibrinogen concentration that is several times greater than the concentration of fibrinogen in blood and a platelet concentration that is greater than the concentration of platelets in blood.
  • Plasma is a water solution of salts, metabolites, peptides, and many proteins ranging from small (insulin) to very large molecules (complement components).
  • the bottom, high-density layer is a deep red viscous fluid comprising anuclear red blood cells (erythrocytes) specialized for oxygen transport.
  • the red color is imparted by a high concentration of chelated iron or heme that is responsible for the erythrocytes' high specific gravity.
  • the relative volume of whole blood that consists of erythrocytes is called the hematocrit, and in normal human beings this can range from about 37% to about 52% of whole blood.
  • the intermediate layer is the smallest, appearing as a thin white band above the erythrocyte layer and below the plasma layer; this is called the buffy coat.
  • the buffy coat itself has two major components, nucleated leukocytes (white blood cells) and anuclear smaller bodies called platelets (or thrombocytes).
  • Leukocytes confer immunity and contribute to debris scavenging. Platelets seal ruptures in blood vessels to stop bleeding, and deliver growth and wound healing factors to a wound site. Slower speed centrifugation or shorter duration centrifugation permits separation of erythrocytes and leukocytes from plasma, while the smaller platelets remain suspended in the plasma, resulting in platelet rich plasma (PRP).
  • PRP platelet rich plasma
  • U.S. Patent No. 5,585,007 identifies methods for making plasma concentrates from whole blood for use in wound healing and as a tissue sealant.
  • This patent is hereby incorporated by reference in its entirety.
  • This device designed for placement in a medical laboratory or surgical amphitheatre, uses a disposable cartridge for preparing tissue sealant. The device was particularly applicable for stat preparations of autologous tissue sealants. Preparation in the operating room of 5 ml of sealant from 50 ml of patient blood required less than 15 minutes and only one simple operator step. There was no risk of tracking error because preparation could take place in the operating room during the surgical procedure. Chemicals added could be limited to anticoagulant (e.g., citrate) and calcium chloride.
  • anticoagulant e.g., citrate
  • the disposable cartridge could fit in the palm of the hand and was hermetically sealed to eliminate possible exposure to patient blood and to ensure sterility.
  • Adhesive and tensile strengths of the product were comparable or superior to pooled blood fibrin sealants made by precipitation methods.
  • Use of antifibrinolytic agents (such as aprotinin) was not necessary because the tissue sealant contained high concentrations of natural inhibitors of fibrinolysis from the patient's blood.
  • This device used a new sterile disposable cartridge with the separation chambers for each run. Since the device was designed to be used in a normal medical setting with ample power, the permanent components were designed for long-term durability, safety and reliability, and were relatively heavy, using conventional centrifuge motors and accessories.
  • centrifugal devices for obtaining platelet concentrates from blood are described in commonly assigned, copending application Serial No. 10/394,828 filed March 21, 2003 , the entire contents of which are hereby incorporated by reference.
  • This device separates blood into erythrocyte, plasma and platelet layers and selectively removes the platelet layer as a platelet concentrate, that is, platelets suspended in a minimal amount of plasma.
  • the plasma fraction being in an unconcentrated form, is not effective as a hemostat or tissue adhesive.
  • Platelet rich plasma is a concentrated platelet product that can he produced from whole blood through commercially available systems, resulting in varying levels of platelet concentration. Platelets play a crucial role in the signaling cascade of normal wound healing. Activated platelets release the contents of their ⁇ -granules resulting in a deposition of powerful growth factors such as platelet derived growth factor (PDGF), transforming growth factor ⁇ -(TGF- ⁇ ), vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF).
  • PDGF platelet derived growth factor
  • TGF- ⁇ transforming growth factor
  • VEGF vascular endothelial growth factor
  • EGF epidermal growth factor
  • PRPs have demonstrated numerous clinical benefits to patients. There are many devices on the market that concentrate platelets to differing levels. At this time, it is unclear the amount of platelets that is most efficient for each surgical application. Concentrations of at least 1,000x10 3 platelets/ ⁇ L are recommended.
  • the system described in copending Application Serial No. 10/394,828 can provide platelets up to 8 time baseline concentration, and the normal human platelet range is 200x10 3 platelets/ ⁇ L to 400x10 3 platelets/ ⁇ L. This means a highly effective concentrate in a range of 1,600x10 3 platelets/ ⁇ L to 3,200x10 3 platelets/ ⁇ L.
  • the PRP products of the prior invention while achieving greatly increased platelet concentrations, did not have tissue sealant and hemostatic properties needed for many surgeries.
  • the device of this invention is a PRP separator-concentrator comprising a housing, a PRP separation assembly, and a PRP concentration assembly.
  • the concentration assembly has a PRP concentration sump.
  • An axially concentric rigid stationary outlet tube is secured to the housing and extends through the PRP separation assembly to the PRP concentrate sump.
  • the PRP separation assembly is attached to and positioned above the PRP concentration assembly to form a combined separator-concentrator assemblage that is rotatable about the outlet tube.
  • the PRP separation assembly can comprise a separation chamber having an outer wall with an inner wall surface and a sloped floor secured to the outer wall, the inner wall surface being lined with a depth filter having pores and passageways that are sized to receive and entrap erythrocytes during centrifuging.
  • the PRP separation assembly includes a blood inlet.
  • the separation chamber can include a top plate and a balanced distribution of separator plates attached to the outer wall and floor of the separation chamber, the separator plates lying in a plane that is parallel to the central axis.
  • the separator plates can extend from the outer wall radially inward to a distance beyond the surface of the depth filter and from the floor to a position spaced from the top plate.
  • the separation chamber is balanced for substantially vibration-free rotation about the central axis.
  • the PRP concentrator can comprise a concentration chamber having a floor for supporting desiccated beads and a wall with at least one opening closed with a screen.
  • the screen has openings that are sized to retain the desiccated beads in the concentration chamber.
  • the concentration chamber can be surrounded by an outer wall with a sloped floor secured thereto, the sloped floor including at its center, a PRP concentrate sump.
  • the concentrator can have a distribution of upright screen supports, the upright screen supports having an inner surface and an outer surface, the cylindrical screen being supported on the outer surface of the upright screen supports.
  • a stationary bead rake can be secured to the stationary tube and extend outward therefrom, the rake having distal ends that are spaced at a distance from the upright screen supports.
  • the rake can comprise a longitudinal body, the center of which is secured to the rigid outlet tube.
  • the longitudinal body can optionally have weakened fracture points adjacent to the rigid tube, whereby the longitudinal body fractures when it is exposed to excessive strain from swelled bead contact during high speed centrifugation.
  • the concentration assembly can have secured to its bottom, an axially concentric concentrator drive coupling, the PRP separator-concentrator including a motor assembly with a motor coupling that engages the concentrator drive coupling.
  • the motor assembly can comprise a motor control system for timed rotations of the drive coupling during an acceleration phase, a rapid centrifugal erythrocyte separation phase, a deceleration phase, a slow stir concentrating phase, an acceleration phase, and a rapid centrifugal PRP concentrate separation phase.
  • the PRP separator-concentrator of this invention can include a valve assembly and a central passageway connecting the separation chamber and the concentration chamber, the upper surface of the central passageway including a valve seat.
  • the valve seat includes a valve face that forms a seal with the valve seat in the close position and separates to disengage the seal in the open position.
  • the valve assembly can include a pair of opposed normally upright valve operator arms, each operator arm having an inflexible body with a weighted distal end and a flexible proximal end. Each flexible proximal end can be secured to the valve face at a level that elevates the valve face in an axial direction to move the valve face to the open position when the operator arms pivot outward under centrifugal force during fast rotation of the separator-concentrator about its central axis.
  • the flexible proximal ends can be positioned between opposed plates extending upward from the floor of the separation assembly, each plate having plate side edges, the plate side edges being positioned to contact the operator arms and thereby restrain the proximal ends against rotation around the central axis when the arms are in the upright position and to free the operator arms from rotation when the flexible proximal ends are raised above the plate side edges when the valve is opened.
  • the plates can have a top edge that is positioned to support the operator arms after their axial rotation, thereby preventing their return to the upright position when centrifugal rotation is ended, thereby preventing closure of the valve assembly.
  • the method of this invention for preparing PRP concentrate comprises the steps of preparing PRP from patient blood by capturing patient blood erythrocytes in a depth filter and preparing PRP concentrate by absorbing water in the PRP with absorbent beads.
  • the method includes capturing the erythrocytes by rotating blood at centrifugal speeds in a balanced cylindrical separation chamber that is lined with the depth filter, the separation chamber and depth filter being segmented by radially extending plates into separation zones, the plates maintaining substantially balanced distribution of the blood in the separation zones during rotation of the separation chamber, thereby reducing vibration and erythrocyte displacement from the depth filter.
  • the rotational speed of the separation chamber can be accelerated to centrifugal speeds at a rate that allows balanced distribution of blood in the separation zones, and after the centrifuging is complete, the rotation speed of the separation chamber can be decelerated to below centrifugal speeds at a rate that allows balanced distribution of the PRP in the separation zones, thereby reducing vibration and erythrocyte displacement from the depth filter.
  • the PRP can be contacted in a rotating concentrating chamber with desiccated beads to produce PRP concentrate while the beads are stirred with a stationary rake.
  • the PRP concentrate can be collected by rotating the concentration chamber at centrifugal speeds to separate PRP concentrate from the beads.
  • the method for preparing PRP concentrate can comprise the steps of preparing PRP from patient blood by capturing patient blood erythrocytes in a depth filter, and preparing PRP concentrate by absorbing water in the PRP with absorbent beads.
  • PRP concentrate can be produced by contacting PRP with desiccated beads in a rotating concentrating chamber while the beads are stirred with a stationary rake.
  • the PRP concentrate can be collected by rotating the concentration chamber at centrifugal speeds to separate PRP concentrate from the beads.
  • the apparatus and method of this invention prepares a novel PRP concentrate that combines enhanced platelet levels in a plasma concentrate in which the fibrinogen levels have not been significantly denatured.
  • the novel product combines the sealant and haemostatic properties of the plasma concentrates greatly valued in certain types of surgery with the enhanced healing properties provided by elevated platelet levels.
  • Fig. 1 is a cross-sectional view of a disposable separation and concentration assembly and a permanent drive assembly, with desiccated beads shown in half of the concentration subassembly. Details of the subsections of this assembly are hereinafter described in conjunction with more detailed drawings.
  • the upper housing 2 is described in greater detail hereinbelow in conjunction with Figs. 2 and 3 .
  • the motor drive subsystem 4 is described together with the motor drive system in conjunction with Figs. 22-24 .
  • the separation system 3 enclosed in the upper housing 2 is described in greater detail with regard to Fig. 4 .
  • the separation system comprises a combination of subsystems including the outer cap subassembly 6 described in greater detail with respect to Figs. 5-7 ; a top bucket 8 described in greater detail with regard to Figs. 8 and 9 ; a sample inlet subassembly shown in Fig. 10 ; a top bucket cap subassembly 10 described in greater detail with respect to Figs. 11 and 12 ; and a valve subassembly 12 described in greater detail with respect to Figs. 13 and 14 .
  • the concentrating system 11 includes a lower bucket 14 and drive connector 16, described in greater detail with regard to Figs. 15 and 26 ; a basket subassembly 18 described in greater detail with regard to Figs. 17 and 18 ; and a mixer assembly described in greater detail with regard to Figs. 19 to 21 .
  • Fig. 2 is a front view of the outer housing of the separation-concentration assembly of this invention
  • Fig. 3 is a perspective view of the outer housing of Fig. 2 showing details of the motor assembly connector.
  • the upper housing 2 isolates the sterile separation and concentration systems shown in Fig. 1 .
  • the upper portion of the outer housing 2 is sealed with an outer cap subassembly 34 having a blood inlet tube 86 and a PRP concentrate outlet port 62 and cap 66.
  • the lower assembly connector has a drive recess 42 shaped to engage the motor subassembly, and with spacer receptors 44 for holding spacers 46.
  • the outer housing 2 and its enclosed separation components are a disposable unit to be used with a permanent drive assembly shown in Figs. 1 and 22 to 24 .
  • the lower assembly includes an axially concentric motor drive receptor 48 and a plurality of tapered engagement and locking slots 50 that engage with corresponding mounting projections of the motor drive assembly (not shown).
  • Fig. 4 is a cross-sectional drawing of the separation-concentration sub-assemblies shown in Fig. 1 .
  • the outer housing 2 encloses an upper separation subassembly 3 and a lower concentration subassembly 11.
  • the top of the outer housing 2 is closed with outer cap subassembly 6 shown in greater detail with regard to Figs. 5-7 .
  • the outer cap subassembly 6 comprises a circular cap 56 with an annular flange 58 extending downward for securing it to the top of the upper housing 2.
  • Concentrate outlet conduit 60 passes through an outlet conduit hole 62 in the center of the plate 56, extending through the plate and communicating with the separation chamber 64 ( Fig. 4 ).
  • Circular cap 66 has a central receptor 68 that engages with a Luer fitting 70 on the upper end of the outlet conduit 60 to maintain a sterile closure during the separation process.
  • An inlet port hole 72 is positioned in the circular cap 56, spaced from the central axis.
  • the inlet port hole 72 is sized to engage the exterior inlet conduit 74 shown in Fig. 4 .
  • the Luer fitting 70 is provided to engage an empty applicator syringe for removing platelet rich plasma concentrate product according to this invention.
  • the lower end of the concentrate outlet conduit 60 constitutes a receptor for receiving the upper end of rigid tube 74 ( Fig. 4 ).
  • Fig. 8 is a top view of the top bucket cap subassembly 10 of the separation-concentration assembly shown in Fig. 4
  • Fig. 9 is a cross-sectional view of the top bucket cap subassembly shown in Fig. 10 , taken along the line 9-9.
  • the cap subassembly 10 closes the top separation bucket 8 shown in greater detail with respect to Figs. 11 and 12 .
  • the top bucket cap 10 comprises a circular plate 76 with a connecting flange 78 that extends downward from the lower edge of plate 76. While the upper plate 6 is fixed to the outer housing 2 ( Fig. 4 ) and is stationary during the separation and concentration processes, top bucket cap 10 is secured to the top bucket 8 for rotation with the top bucket 8 during the separation and concentration processes.
  • the circular cap 10 has an axially concentric hole with a valve assembly guide tube 80 extending downwardly therefrom.
  • the lower end of the guide tube 80 has a valve assembly stop flange 82 secured thereto.
  • the upper end of the guide tube 80 supports sleeve bearing 84.
  • the circular cap 10 has a sample inlet subassembly 86 that aligns with the hole 72 in the circular cap 56 ( Fig. 5 ).
  • Fig. 10 is an exploded view of the sample inlet subassembly 86.
  • the sample inlet subassembly 86 comprises an inlet tube 92 mounted in the plate 76, the top of the inlet tube 92 including an annular receptor 94.
  • a sterile filter 96 is positioned in the lower end of the passageway 97 of tube 92.
  • the subassembly 86 includes a removable inlet tube 98.
  • Inlet tube 98 comprises a central tube 100 having at its upper end an integral Luer fitting 102.
  • annular plate 103 extends outward from the tube.
  • An integral cylindrical flange 104 extends downward from the outer edge of the plate 103. The flange 104 is sized to engage the receptor 94.
  • the lower end 105 of the tube 100 is sized to engage the upper end of the passageway 97.
  • the inlet tube is provided with a cap 106 that engages the Luer fitting 102 to provide a sterile closure of the removable inlet tube 98 during shipment and handling prior to use.
  • the inlet tube 98 in passing through the hole 72 in the stationary circular cap 56 locks the separation and concentration subassemblies against rotation during shipment and storage. After the patient blood is introduced into the top bucket 8 ( Fig. 4 ) through the inlet subassembly 86, the inlet tube 98 is removed, unlocking the separation and concentration sub-assemblies 3 and 11 from the stationary circular cap 6, freeing them for rotation about the central tube 74.
  • a sterile breathing tube 108 is secured to the circular plate 76 to permit air flow from the separation chamber 64 when blood is introduced and to permit air movement into the system when platelet-rich concentrate is removed from the concentrating system 11, as described in greater detail hereinafter.
  • Sterile air filter 110 in breathing tube 108 ( Fig. 9 ) prevents entrance of microorganisms into the interior of the separation chamber, preserving sterility.
  • Fig. 11 is a top view of the top bucket subassembly 10 of the separation-concentration assembly shown in Fig. 4
  • Fig. 12 is a cross-sectional view of the top bucket subassembly of Fig. 11 , taken along the line 12-12.
  • the top bucket subassembly 10 comprises a cylindrical outer wall 112 having a top edge 114 that is secured to the inner surface of the flange 58 of the upper bucket cap 10.
  • the lower end of the cylindrical outer wall 112 is closed with integral sloped floor plate 116 with a central passageway 118 that constitutes a central flow passageway for separated platelet-plasma.
  • the inner wall surface of the passageway 118 constitutes a valve seat 119 for the valve assembly described in greater detail hereinafter with respect to Figs. 13 and 14 .
  • vent columns 120 Spaced from the central passageway 118 and secured to the floor plate 116 are vent columns 120 with filters 122 in their bottom.
  • the columns 120 serve as vents allowing movement of air from the concentration subassembly into the separation chamber when liquid flows through downward through the central passage 118, as is explained hereinafter.
  • Filters 122 prevent escape of hydrogel beads from the basket subassembly 18 through the vent columns 120 during transport or handling of the device of this invention.
  • Surrounding the central passageway 118 and secured to the upper surface of the tapered floor plate 116 are upwardly extending abutment plates 124, each having an upper valve arm abutment surface 128.
  • a plurality of radially inwardly extending separation plates 130 are secured to the inner surface of the cylindrical outer wall 112 and the sloped floor plate 116. Each adjacent pair of these plates defines a separation zone 132.
  • the plates 130 must be evenly spaced around the cylindrical outer wall to provide a balanced subassembly. They can be in matched, opposed pairs, for example the three matched sets as shown in Fig. 11 .
  • the top edge 134 of each of the separation plates 130 is spaced at a distance below the top edge 114 to permit overflow of blood in order to achieve an even distribution of blood between each the separation zones 132 during the spin acceleration stages and during the spin deacceleration stages, thus maintaining balance and minimizing vibration of the rotating assembly.
  • the interior surface 136 of the cylindrical outer wall segments in each of the each separation zones 132 is lined with an open-cell foam segment or depth filter segment 138.
  • the foam segments 138 have pores and passageways sized to allow infiltration of erythrocytes into the foam and subsequent entrapment of erythrocytes during the high speed centrifugation of the separation stage.
  • the pores and passageways are sized to retain entrapped erythrocytes thereafter when the spinning slows or stops and the erythrocyte-free platelet-plasma suspension flows downward through the opening 118.
  • Fig. 13 is a front view of the valve assembly 12 of the separation-concentration assembly shown in Fig. 4
  • Fig. 14 is an exploded, isometric view of the valve assembly of Fig. 13
  • the valve assembly 12 comprises a central tube 140, the lower end constituting a valve face 142.
  • the valve face 142 comprises an annular receptor 144 that receives and holds an O-ring 146.
  • the outermost surface of the O-ring 146 is sized to form a sealing engagement with the valve seat 119 (See Figs. 11 and 12 ).
  • the valve assembly 12 includes two opposed centrifugal arms 148 secured to the tube 140 above the valve face 142. Each centrifugal arm 148 has a flexible portion 150 adjacent the tube 140 and a rigid arm portion 152. The distal end of the rigid arm portion 152 includes a weight receptor 154 in which a weight 156 is secured to provide additional weight to the end of the rigid arm portion. Operation of the valve assembly is described hereinafter with respect to Figs. 25-31 .
  • the lower bucket 14 in Fig. 4 is shown in detail in Figs. 15 and 16 .
  • the lower bucket 14 has a cylindrical sidewall 158 and a sloped bucket bottom 160, the lower portion of which forms a platelet-plasma concentrate sump 162 in which concentrated platelet and plasma concentrate collects.
  • a plurality of basket supports 164 extend upward from the top surface of the slopped bucket bottom 160, the top surfaces 166 of which support a concentrating basket subassembly 18 described hereinafter with regard to Figs. 17 and 18 .
  • An axially concentric drive receptor 168 shown in detail in Fig. 16 is secured to the bottom surface of the slopped bucket bottom 160.
  • the drive connector receptor 168 can have any configuration that will releasably couple with a suitably configured motor drive connector.
  • the drive receptor 168 comprises an outer cylinder 170 and a plurality of ridges 172, each ridge having a tapered leading engagement surface 174, an abutment surface 176 and an upper plate 178.
  • the upper plate 178 transmits the torque from the drive motor (described hereinafter with respect to Figs. 22-24 ) to the lower bucket bottom 160 and from there to the concentrating and separating subassemblies, all of which are secured together to form a unitary rotatable assembly.
  • Fig. 17 is a front view of the basket subassembly 18 of the separation-concentration assembly shown in Fig. 4
  • Fig. 18 is a cross-sectional view of the basket subassembly of Fig. 17 , taken along the line 18-18.
  • the basket subassembly 18 comprises a cylinder 180 secured to a circular floor plate 182.
  • a slip bearing 184 is positioned in the axial center of the circular plate 182 for engaging the rigid tube 74 ( Fig. 4 ).
  • the cylinder 180 has an array of windows 186 around its circumference, each window closed with a fine screen 188 having a mesh size sufficiently small to prevent escape of hydrogel beads 19 ( Figs. 1 and 4 ) from the basket during spinning.
  • Fig. 19 is a top view of the mixer assembly of the separation-concentration assembly shown in Fig. 4 .
  • Fig. 20 is a cross-sectional view of the mixer assembly of Fig. 18 , taken along the line 20-20, and
  • Fig. 21 is an isometric view of the mixer assembly of Figs. 19 and 20 .
  • the mixer assembly 20 comprises a rake 190 secured to stationary tube 74.
  • the upper end 192 of the stationary tube 74 is secured to the upper cap subassembly 34 to secure it against rotation.
  • the lower end 194 of the stationary tube 74 is an inlet port for removal of platelet-plasma concentrate from the sump 162 ( Fig. 15 ).
  • the rake 190 comprises a radially extending spine 196 from which integral rake elements 198 extend downward to an elevation short of the bottom plate 182 of the basket subassembly 18 as shown in Figs. 4 , 17 and 18 .
  • the spine 196 can have optional breakaway notches 200 adjacent its center. The notches 200 weaken the spine and direct fracture of the spine 196 at the location of the notches if the event that the pressure produced by contact by beads 19 with the rake elements 198 during the final centrifugal spin become excessive.
  • the stationary tube 74 extends through the sleeve bearing 184 of the basket subassembly 18 and through the sleeve bearing 84 of the top bucket cap, permitting free rotation of the separating and concentrating assemblies around the stationary tube.
  • the stationary tube 74 is fixed to the outer cap subassembly 6 and the stationary outer housing 2.
  • Fig. 4 is a comprehensive assemblage of the components shown in Figs. 5-20 .
  • Concentrating desiccated hydrogel beads 19 fill the lower half of the basket 18 (only one side is shown empty to enable unobstructed viewing of the windows 186 and screen 188 elements ( Figs. 17 and 18 ).
  • the concentrating desiccated hydrogel beads 19 can be insoluble beads or disks that will absorb a substantial volume of water and low molecular weight solutes while excluding high molecular weight solutes and particulates and will not introduce undesirable contaminants into the plasma. They can be dextranomer or acrylamide beads that are commercially available (Debrisan from Pharmacia and BIO-GEL PTM from Bio-Rad Laboratories, respectively). Alternatively, other concentrators can be used, such as SEPHADEXTM moisture or water absorbents (available from Pharmacia), silica gel, zeolites, crosslinked agarose, etc., in the form of insoluble inert beads.
  • Fig. 4 in conjunction with subassembly Figs. 5-21 shows the assembly prior to use with the valve assembly 12 secured for shipment by the sleeve 80 into which the valve assembly tube 140 extends and the abutment flange 82 secured to the bottom of the sleeve 80.
  • the valve face 142 is shown in position against the seat 119. This confines the beads to the basket 18 and prevents escape of beads into the upper separation chamber 64 if the device is inverted or shaken during transport or handling.
  • the assembly is secured against rotation around the rigid tube 74 by the position of the removable inlet tube 98 in the hole 72 of the stationary outer cap subassembly 6.
  • the upper edge of the cylinder 180 of the basket assembly 18 is secured against the lower surface of the tapered bottom 116, and the lower surface of the plate182 is secured against the upper edge surfaces 166 ( Fig. 15 )of the supports 164.
  • the upper separation subassembly 3 and the lower concentration subassembly 11 rotate as a single unit around the fixed tube 74.
  • the upper separation subassembly is positioned on the central tube 74 by the slip bearing 84 through which the fixed tube 74 extends.
  • the lower separation subassembly is positioned on the central tube 74 by the slip bearing 184 through which the fixed tube extends.
  • the rake assembly 20 including the tube 74 remain stationary during rotation of the separation and concentration subassemblies 3 and 11 in the separation and concentration phases, to be described in greater detail hereinafter.
  • Fig. 22 is a perspective view of the motor drive assembly of this invention.
  • Fig. 23 is a cross-sectional view of the motor drive assembly taken along the line 23-23, and
  • Fig. 24 is a cross-sectional view of the motor drive assembly taken along the line 24-24.
  • the outer shell 202 of the motor housing 4 encloses the motor 218 and supports the control interface 204 and the power connector 206.
  • the separation-concentrating assemblies are supported on the raised annular support surface 208 surrounding the motor connector 210.
  • Motor connector 210 has a configuration that will releasably engage the drive receptor 168 ( Fig. 16 ).
  • the bottom of the housing 22 is closed by support plate 212.
  • a control and power plate 214 for the system is supported by four support struts 216 attached to the underside of the housing shell 202.
  • Plate 214 is a conventional printed circuit or equivalent board with the electronic components of the control and power system for the device, and in its center, a support 217 for the motor 218.
  • the electrical components are connected to the control interface 204 and power connector 206 by conventional wiring circuits (not shown).
  • Four support feet 220 are secured to the bottom of the support plate 212 and provide friction surfaces 222 to secure the device on a laboratory surface.
  • Figs. 25-31 illustrate the operation of the valve subassembly during and immediately after the initial separation process. Blood and blood products are omitted from these cross-sectional views to allow an unobstructed view of the valve assembly elements at each stage.
  • Fig. 25 is a cross-sectional view of the upper bucket and valve subassembly of Fig. 4 , taken along the central axis
  • Fig. 26 is a cross-sectional view of the upper bucket and valve assembly of Fig. 25 , taken along the line 26-26. This is the view when blood is initially introduced into the top bucket 8.
  • the arms 148 of the valve subassembly are in their initial upright position, with the central tube 140 positioned in the guide tube 80 and the upper end of each arm contacting the flange 82.
  • the valve face 142 is in position in the valve seat 119 ( Fig. 12 ) at the upper end of the central passageway 118, closing the passageway and preventing escape of blood.
  • the flexible portions 150 of the arms 148 are positioned in the channels between the abutment plates 124 and 126, preventing rotation of the arms 148 about tube 74 during shipment and handling.
  • Fig. 27 is a cross-sectional view of the upper bucket and valve assembly of Figs. 25 and 26 , after the centrifugal action of the spinning upper bucket has extended the arms of the valve assembly and opened the valve
  • Fig. 28 is a cross-sectional view of the view of upper bucket and valve assembly of Fig. 27 , taken along the line 28-28.
  • valve arms 148 rotate outward until they contact the sloped floor 116. This action slides the valve central tube 140 upward to the upper portion of the guide cylinder 180, pulling the valve face 142 from the central passageway 118 and out of contact with the valve seat 119 to open the passageway 118. As the arms 148 rotate outward and the valve face 142 is lifted, the lower flexible ends 150 of the arms 148 are also pivoted upward from between the abutment plates 124 and 126, freeing the arms for rotation about the tube 74. Because the liquid is held against the foam segments 138 by centrifugal force, it does not flow through the open passageway 118.
  • Fig. 29 is a cross-sectional view of the upper bucket and valve assembly of Figs. 27 and 28 , after rotational displacement of the arms of the valve assembly
  • Fig. 30 is a cross-sectional view of the valve structure of Fig. 29 , taken along the line 30-30.
  • Fig. 31 is a cross-sectional view of the upper bucket and valve assembly of Fig. 29 and 30 , after centrifugal separation has been completed and the rotation of the separation and concentration subassemblies is slowed or stopped.
  • the platelet-plasma mixture flows to the bottom of the tapered floor 116, down its sloped surface to the central passageway 118, and through the central passageway 118 to the basket subassembly 18 for concentration.
  • the removal of the strong centrifugal action may permit the arms 148 to spring upward, causing the valve face 142 to move downward toward the central passageway 118. This movement is stopped when one or both flexible arm portions 150 contact an opposed abutment surface 128, leaving the central passageway open to the flow of the platelet-plasma mixture.
  • Fig. 32 is a cross-sectional view of the separation and concentration assemblies of Fig. 4 , after blood 202 has been introduced into the separation assembly 3 through the tube 110 from a syringe secured to the Luer fitting 102.
  • the upper tube 100 with the Luer fitting is then removed, unlocking the separation and concentration assemblies 3 and 11 for rotation.
  • the blood flows into the bottom of the top bucket 8. Air displaced by the incoming liquid escapes through breathing tube 108.
  • the valve face 142 is in a closed position, preventing escape of the blood from the bucket 8.
  • the operation of the system is then initiated, and the motor 218 spins the separation and concentration assemblies together around the rigid tube 74.
  • Fig. 33 is a cross-sectional view of the separation and concentration assemblies of Fig. 32 as erythrocytes are separated from the plasma-platelet mixture during high speed centrifugation.
  • the separation and concentration assemblies turn at a high speed, the blood is forced against the foam 138.
  • the erythrocytes being more dense than other blood components, preferentially migrate into the pores and passageways of the foam.
  • the valve subassembly opens the valve 142 as the centrifugal forces pivot the outer ends of the arms 148 away from the center, raising the valve face 142 face from valve seat 119 in the central passageway 118.
  • all of the liquid is maintained against the foam.
  • centrifugal forces also force the hydrogel beads 19 radially outward against the outer screens 188 of the basket subassembly, out of contact with elements of the rake 190. Centrifugation is continued until a majority of the erythrocytes a completely trapped in the foam. Because any erythrocytes weaken the gel product formed when the product of this invention is applied, the removal of a maximum proportion of the erythrocytes is desired. The speed of centrifugation tends to separate erythrocytes from platelets, leaving a substantial portion of the platelets in the plasma while entrapping a majority of the erythrocytes in the foam.
  • Fig. 34 is a cross-sectional view of the separation and concentration assembly of Fig. 33 .
  • the platelet-plasma fraction 204 flows to the bottom of the upper bucket 8 and down through the central passageway 118 into the basket subassembly 18 where it comes into contact with the desiccated hydrogel beads 19.
  • These beads concentrate the plasma by absorbing water from the liquid.
  • the separation and concentrating assemblies are then rotated at a slow speed by the motor 218, stirring the beads by moving them through the stationary spines 196 of the rake 190. Agitating the beads insures maximum contact of the beads surfaces with the plasma and reduces gel polarization that arises when the plasma thickens adjacent the bead surfaces. This phase is continued until the desired proportion of the water has been removed and the desired concentration of the plasma has been achieved.
  • Fig. 35 is a cross-sectional view of the separation and concentration assembly of Fig. 34 at the beginning of high speed centrifugation separation of the platelet-plasma concentrate from the hydrogel beads.
  • the separation and concentration assemblies are rapidly rotated by the motor 218 around the stationary tube 74, creating centrifugal forces that force the platelet rich plasma concentrate and the beads 19 against the screen elements 188 of the basket 18.
  • the screen elements prevent escape of the beads 19 as the continuing centrifugal force causes the platelet enriched plasma concentrate to flow from the beads and through the screen. This high speed centrifugation is continued until a maximum recovery of the platelet rich plasma is obtained.
  • the absorption of water by the hydrogel beads is accompanied by an increase in bead diameter, increasing the bead volume. If the increased bead volume causes the ends of the rake 190 to drag on beads packed on the screen surface, the rake breaks along the break-away notches 200 ( Fig. 19 ), and the rake fragments become mixed with the beads.
  • Fig. 36 is a cross a cross-sectional view of the separation and concentration assembly of Fig. 35 after the high speed centrifugation has ended and the platelet-plasma concentrate has flowed into the platelet-plasma concentrate sump 162.
  • the cap 66 has been removed, exposing Luer fitting 70 at the upper end of the tube 60.
  • An applicator syringe (not shown) is secured to the Luer fitting 70.
  • the platelet-rich plasma concentrate is removed from the sump 162 by retracting the barrel of the applicator syringe, drawing platelet rich plasma concentrate up through the tubes 74 and 60 and into the syringe.
  • Breathing tube 108 permits air to flow into the system to replace the volume of liquid removed by the syringe, thus preventing the creation of a partial vacuum in the system that would impede liquid removal.
  • concentration factor for maximum wound-healing, the platelet level is maximized and high concentrate ion factors are sought.
  • concentration factor for maximum wound-healing, the platelet level is maximized and high concentrate ion factors are sought.
  • plasma concentrations of 3 to 4 fold over anti-coagulated plasma levels are most effective. Concentrations below 3 fold have an insufficient fibrinogen concentrate ion. Concentrations higher than 4 old have excessive levels of total protein (principally albumin) which interferes with the fibrin gel structure.
  • a concentration range of 3 to 4 fold over anti-coagulated plasma levels is a best choice. For applications where sealant activity is not desired, high concentrations may be preferred.
  • erythrocyte levels normal human hematocrits vary from 37 percent or lower to about 52 percent for whole blood, measured after a very high speed spin. To achieve concentrations of 3 fold or higher, some erythrocyte removal is necessary. However, the tensile strength of concentrated plasma gels diminish as the level of erythrocyte contamination increases. The concentration of erythrocytes in the final concentrate should be less than 3 to 5 percent to provide effective haemostatic properties.
  • the device of this invention is intended to remove as much of the erythrocytes as is technically practical with the system, although trace contamination is accept able. For applications where sealant activities are not desired, higher levels of erythrocytes are tolerable.
  • both the depth filter and the beads reduce the liquid volumes being processed. Because of this volume loss, only from 14 to 17 percent volume yields of effective haemostatic wound-healing product is generally obtained from average patient blood with the device of this invention.
  • the depth filter volume is selected to retain about 50 percent of the anti-coagulated blood (blood containing anticoagulant) and product about a 50 percent yield of PRP.
  • the amount of the beads, in water absorption units, is selected to retain water equaling about 67 percent of the PRP volume.
  • the amount of the depth filter and beads in each system is carefully selected to yield an optimum product.
  • accuracy the amount of the depth filter and beads in each system is carefully selected to yield an optimum product.
  • the volume retained by the depth filter is about half the total volume of blood to be processed, if the volume of blood introduced into the device is too small, a substantially lower volume of PRP will be delivered to the beads. For example, if the blood volume is low by only 25 percent, this will result in only 50 percent of the desired volume being delivered to the beads. If the volume of PRP contacting the beads is low by 33 percent or more, no product will be recovered because the beads will always absorb 67 percent of the targeted PRP volume.
  • the systems can be designed to specifically match the hematocrit levels of the particular patient's blood to be processed.
  • the device is optimized for the average patient blood, using fixed volumes of depth filter and blood, and a fixed bead water absorption capacity.
  • the device can incorporate an overflow chamber as described in provisional patent application Serial No. 60/654,718 filed February 17, 2005 and concurrently filed application Attorney Docket No. 426.P009B, the contents of which are hereby incorporated by reference.

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EP15159104.7A 2005-02-07 2006-01-30 Appareil de concentration de plasma riche en plaquettes Expired - Lifetime EP2910258B1 (fr)

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US65105005P 2005-02-07 2005-02-07
US65471805P 2005-02-17 2005-02-17
US72331205P 2005-10-04 2005-10-04
PCT/US2006/003597 WO2006086199A1 (fr) 2005-02-07 2006-01-30 Appareil et procede de preparation de concentres de plasma riche en plaquettes
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Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7992725B2 (en) * 2002-05-03 2011-08-09 Biomet Biologics, Llc Buoy suspension fractionation system
US7832566B2 (en) 2002-05-24 2010-11-16 Biomet Biologics, Llc Method and apparatus for separating and concentrating a component from a multi-component material including macroparticles
US20030205538A1 (en) 2002-05-03 2003-11-06 Randel Dorian Methods and apparatus for isolating platelets from blood
WO2003099412A1 (fr) 2002-05-24 2003-12-04 Biomet Manufacturing Corp. Appareil et procede pour separer et concentrer des fluides contenant de multiples composants
US7845499B2 (en) 2002-05-24 2010-12-07 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US20060278588A1 (en) 2002-05-24 2006-12-14 Woodell-May Jennifer E Apparatus and method for separating and concentrating fluids containing multiple components
US7354515B2 (en) 2004-02-23 2008-04-08 Millennium Medical Technologies, Inc. Fluid concentrator
US7708152B2 (en) 2005-02-07 2010-05-04 Hanuman Llc Method and apparatus for preparing platelet rich plasma and concentrates thereof
US7866485B2 (en) 2005-02-07 2011-01-11 Hanuman, Llc Apparatus and method for preparing platelet rich plasma and concentrates thereof
ES2426941T3 (es) 2005-02-07 2013-10-25 Hanuman Llc Aparato y procedimiento de concentrados de plasma rico en plaquetas
US7640301B2 (en) * 2006-04-06 2009-12-29 Att Knowledge Ventures, L.P. System and method for distributing video conference data over an internet protocol television system
US8567609B2 (en) 2006-05-25 2013-10-29 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
CA2677581C (fr) * 2007-02-06 2018-11-13 Yoav Kimchy Detection intra-lumenale de polypes
US8034014B2 (en) 2007-03-06 2011-10-11 Biomet Biologics, Llc Angiogenesis initation and growth
US8328024B2 (en) 2007-04-12 2012-12-11 Hanuman, Llc Buoy suspension fractionation system
JP5479319B2 (ja) 2007-04-12 2014-04-23 バイオメット・バイオロジックス・リミテッド・ライアビリティ・カンパニー ブイ式懸濁液分画システム
US20080269762A1 (en) * 2007-04-25 2008-10-30 Biomet Manufacturing Corp. Method and device for repair of cartilage defects
US7901344B2 (en) * 2007-05-11 2011-03-08 Biomet Biologics, Llc Methods of reducing surgical complications in cancer patients
US20090192528A1 (en) * 2008-01-29 2009-07-30 Biomet Biologics, Inc. Method and device for hernia repair
EP2567692B1 (fr) 2008-02-27 2016-04-06 Biomet Biologics, LLC Utilisation d'un dispositif pour obtenir des solutions riches en antagoniste de récepteur d'interleukine-1
US8753690B2 (en) 2008-02-27 2014-06-17 Biomet Biologics, Llc Methods and compositions for delivering interleukin-1 receptor antagonist
WO2009111338A1 (fr) 2008-02-29 2009-09-11 Biomet Manufacturing Corp. Système et procédé pour la séparation d'une matière
US7850917B2 (en) * 2008-03-11 2010-12-14 Ortho-Clinical Diagnostics, Inc. Particle agglutination in a tip
US8012077B2 (en) 2008-05-23 2011-09-06 Biomet Biologics, Llc Blood separating device
US8309343B2 (en) 2008-12-01 2012-11-13 Baxter International Inc. Apparatus and method for processing biological material
US8177072B2 (en) 2008-12-04 2012-05-15 Thermogenesis Corp. Apparatus and method for separating and isolating components of a biological fluid
US8187475B2 (en) 2009-03-06 2012-05-29 Biomet Biologics, Llc Method and apparatus for producing autologous thrombin
US8313954B2 (en) 2009-04-03 2012-11-20 Biomet Biologics, Llc All-in-one means of separating blood components
US8540078B2 (en) 2009-06-16 2013-09-24 Biomet Biologics, Llc Liquid separation from adipose tissue
US8790519B2 (en) * 2009-06-16 2014-07-29 Biomet Biologics, Llc Liquid separation from adipose tissue
US9011800B2 (en) 2009-07-16 2015-04-21 Biomet Biologics, Llc Method and apparatus for separating biological materials
US20110052561A1 (en) * 2009-08-27 2011-03-03 Biomet Biologics,LLC Osteolysis treatment
US9763875B2 (en) 2009-08-27 2017-09-19 Biomet Biologics, Llc Implantable device for production of interleukin-1 receptor antagonist
KR101080290B1 (ko) 2010-01-22 2011-11-08 은형일 자가혈 필러 제조방법
US8591391B2 (en) 2010-04-12 2013-11-26 Biomet Biologics, Llc Method and apparatus for separating a material
AU2011296356B2 (en) 2010-09-03 2015-07-09 Biomet Biologics, Llc Methods and compositions for delivering interleukin-1 receptor antagonist
US9555171B2 (en) 2010-09-30 2017-01-31 Depuy Mitek, Llc Methods and devices for collecting separate components of whole blood
US8317672B2 (en) 2010-11-19 2012-11-27 Kensey Nash Corporation Centrifuge method and apparatus
US8870733B2 (en) 2010-11-19 2014-10-28 Kensey Nash Corporation Centrifuge
US8469871B2 (en) 2010-11-19 2013-06-25 Kensey Nash Corporation Centrifuge
US8394006B2 (en) 2010-11-19 2013-03-12 Kensey Nash Corporation Centrifuge
US20220031925A1 (en) 2010-11-19 2022-02-03 Dsm Ip Assets B.V. Centrifuge
US8556794B2 (en) 2010-11-19 2013-10-15 Kensey Nash Corporation Centrifuge
KR101263820B1 (ko) 2010-12-09 2013-05-13 도병록 재생성 세포 추출장치
KR101016166B1 (ko) 2011-01-05 2011-03-18 문상호 자가 혈소판 농축물질 분리방법
US20130310815A1 (en) * 2011-01-28 2013-11-21 Ysec Company, Limited Fibrin-gel compression device
KR101031460B1 (ko) 2011-02-21 2011-04-26 문상호 자가 혈소판 농축물질 추출용 장치
US9011684B2 (en) 2011-03-07 2015-04-21 Spinesmith Holdings, Llc Fluid concentrator with removable cartridge
US9164079B2 (en) 2011-03-17 2015-10-20 Greyledge Technologies Llc Systems for autologous biological therapeutics
US9011846B2 (en) 2011-05-02 2015-04-21 Biomet Biologics, Llc Thrombin isolated from blood and blood fractions
US9327296B2 (en) 2012-01-27 2016-05-03 Fenwal, Inc. Fluid separation chambers for fluid processing systems
DE102012201717A1 (de) * 2012-02-06 2013-08-08 AusBio Laboratories Co, Ltd. Probenträger-Zentrifuge
US9120095B2 (en) * 2012-03-29 2015-09-01 Biomet Biologics, Llc Apparatus and method for separating and concentrating a component of a fluid
WO2013183797A1 (fr) * 2012-06-05 2013-12-12 주식회사 휴림바이오셀 Dispositif d'extraction de cellule régénérative
US9642956B2 (en) 2012-08-27 2017-05-09 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
AU2013309502B2 (en) * 2012-08-28 2017-04-20 Fenwal, Inc. Spring-open sheeting for fluid processing cassette
US10208095B2 (en) 2013-03-15 2019-02-19 Biomet Manufacturing, Llc Methods for making cytokine compositions from tissues using non-centrifugal methods
US10143725B2 (en) 2013-03-15 2018-12-04 Biomet Biologics, Llc Treatment of pain using protein solutions
US9895418B2 (en) 2013-03-15 2018-02-20 Biomet Biologics, Llc Treatment of peripheral vascular disease using protein solutions
US20140271589A1 (en) 2013-03-15 2014-09-18 Biomet Biologics, Llc Treatment of collagen defects using protein solutions
US9950035B2 (en) 2013-03-15 2018-04-24 Biomet Biologics, Llc Methods and non-immunogenic compositions for treating inflammatory disorders
US9758806B2 (en) 2013-03-15 2017-09-12 Biomet Biologics, Llc Acellular compositions for treating inflammatory disorders
US9878011B2 (en) 2013-03-15 2018-01-30 Biomet Biologics, Llc Treatment of inflammatory respiratory disease using biological solutions
US9833474B2 (en) 2013-11-26 2017-12-05 Biomet Biologies, LLC Methods of mediating macrophage phenotypes
ES2754326T3 (es) 2014-01-31 2020-04-17 Dsm Ip Assets Bv Centrifugadora de tejido adiposo y procedimiento de uso
US10441635B2 (en) 2014-11-10 2019-10-15 Biomet Biologics, Llc Methods of treating pain using protein solutions
US9763800B2 (en) 2015-03-18 2017-09-19 Biomet C. V. Implant configured for hammertoe and small bone fixation
US9713810B2 (en) 2015-03-30 2017-07-25 Biomet Biologics, Llc Cell washing plunger using centrifugal force
US9757721B2 (en) 2015-05-11 2017-09-12 Biomet Biologics, Llc Cell washing plunger using centrifugal force
US10207044B2 (en) 2015-07-29 2019-02-19 Fenwal, Inc. Five-port blood separation chamber and methods of using the same
US10792411B2 (en) 2016-05-09 2020-10-06 Jae-Hyung Robert CHANG Accelerated method for preparing platelet rich plasma
CN111282721A (zh) * 2018-08-08 2020-06-16 田飞标 一种天然水果香精制备用果汁分离设备及分离方法
WO2020174005A1 (fr) 2019-02-28 2020-09-03 The Regenerative Group Ltd. Combinaison régénérative de plasma et de tissu adipeux
US11931674B2 (en) * 2019-04-04 2024-03-19 Natera, Inc. Materials and methods for processing blood samples
IT201900006867A1 (it) * 2019-05-15 2020-11-15 Prometheus Soc A Responsabilita Limitata Metodo e dispositivo di realizzazione di una composizione comprendente plasma arricchito di piastrine, un kit per detto dispositivo di realizzazione, detta composizione stessa e uso di detta composizione
KR102345147B1 (ko) * 2019-12-09 2021-12-30 문상호 다중 혈액유로를 갖는 혈소판 활성화 키트
GB2587257B (en) * 2020-03-09 2021-09-15 Alma Lasers Ltd Lipoaspirate processing
US12220512B2 (en) 2020-04-23 2025-02-11 Hanuman Pelican, Inc. Plasma/cell concentrator apparatus and methods
CN112058174B (zh) * 2020-09-24 2022-05-13 北京大学第三医院（北京大学第三临床医学院） 一种富血小板血浆（prp）喷枪装置
CN112169419A (zh) * 2020-09-28 2021-01-05 廖琼红 一种利用离心力触发压电效应从而过滤菜籽油杂质装置
CN112547319B (zh) * 2020-11-23 2022-03-29 西安大兴医院 一种输血科血液处理装置
US12343738B2 (en) 2021-04-05 2025-07-01 Fenwal, Inc. Continuous flow centrifugation chambers
CN113499624B (zh) * 2021-05-31 2023-02-17 中工武大设计研究有限公司 一种多级混肥过滤系统及其操作方法
CN114773450A (zh) * 2022-04-27 2022-07-22 万绵水 一种生长因子提取装置
EP4445905A1 (fr) 2023-04-14 2024-10-16 Santxa Research S.L.U. Plasma enrichi en plaquettes et molécules de plasma
CN116712775B (zh) * 2023-08-07 2023-10-20 深圳美讯医学检验科技有限公司 一种血液过滤装置
CN117861280A (zh) * 2024-01-23 2024-04-12 淮北矿山机器制造有限公司 一种浓密机布料装置

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585007A (en) 1994-12-07 1996-12-17 Plasmaseal Corporation Plasma concentrate and tissue sealant methods and apparatuses for making concentrated plasma and/or tissue sealant

Family Cites Families (276)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2553004A (en) * 1949-03-11 1951-05-15 Rabatine Michael Dividing protractor
SE169293C1 (fr) 1958-03-13 1959-11-10
US3141846A (en) 1962-04-05 1964-07-21 Western States Machine Co Load control unit for cyclical centrifugal installation
DK113248B (da) 1963-09-02 1969-03-03 Pharmacia Ab Fremgangsmåde til fremstilling af med en eller flere mono- eller dihydroxyalkylgrupper substitueret dekstran.
JPS497539B1 (fr) * 1966-04-13 1974-02-21
US3469369A (en) 1966-12-29 1969-09-30 Bell Telephone Labor Inc Method for preparing and applying a viscous fluid
US3441143A (en) * 1967-02-10 1969-04-29 Marvel Eng Co Plural element filter assembly
US3409165A (en) 1967-04-03 1968-11-05 Olin Mathieson Floating deck
US3508653A (en) * 1967-11-17 1970-04-28 Charles M Coleman Method and apparatus for fluid handling and separation
DE1782612B1 (de) 1968-09-25 1971-05-19 Westphalia Separator Ag Vorrichtung zum einleiten der entschlammung bei selbstreinigenden schammzentrifugen
US3647070A (en) * 1970-06-11 1972-03-07 Technicon Corp Method and apparatus for the provision of fluid interface barriers
USRE32089E (en) * 1970-08-25 1986-03-04 Amicon Corporation Blood fractionating process and apparatus for carrying out same
US3814248A (en) 1971-09-07 1974-06-04 Corning Glass Works Method and apparatus for fluid collection and/or partitioning
US3779383A (en) 1972-04-25 1973-12-18 Becton Dickinson Co Sealed assembly for separation of blood components and method
US3785549A (en) * 1972-07-31 1974-01-15 Haemonetics Corp Centrifuge chuck for disposable, snap-in centrifuge rotor
US4537767A (en) 1973-01-29 1985-08-27 Pharmacia Aktiebolag Method for cleansing fluid discharging skin surfaces, wounds and mucous membranes and means for carrying out the method
SE452109B (sv) 1973-01-29 1987-11-16 Pharmacia Ab Rengoringsmedel for vetskande utvertes sarytor
US3850369A (en) 1973-03-08 1974-11-26 Coulter Electronics Centrifuge for preparing platelet rich plasma
US3879295A (en) * 1973-08-17 1975-04-22 Eastman Kodak Co Vacutainer with positive separation barrier
US4001122A (en) * 1973-08-22 1977-01-04 Telan Corporation Method and device for separating blood components
US3941699A (en) * 1974-02-27 1976-03-02 Becton, Dickinson And Company Plasma separator with centrifugal valve
US3909419A (en) 1974-02-27 1975-09-30 Becton Dickinson Co Plasma separator with squeezed sealant
US3935113A (en) * 1974-02-27 1976-01-27 Becton, Dickinson And Company Serum/plasma separator with centrifugal valve
US3931010A (en) * 1974-02-27 1976-01-06 Becton, Dickinson And Company Serum/plasma separators with centrifugal valves
US3897343A (en) 1974-02-27 1975-07-29 Becton Dickinson Co Plasma separator-hydrostatic pressure type
US3894952A (en) 1974-02-27 1975-07-15 Becton Dickinson Co Serum/plasma separator assembly having interface-seeking piston
US3929646A (en) 1974-07-22 1975-12-30 Technicon Instr Serum separator and fibrin filter
US3931018A (en) * 1974-08-09 1976-01-06 Becton, Dickinson And Company Assembly for collection, separation and filtration of blood
US4059108A (en) 1974-08-15 1977-11-22 Haemonetics Corporation Process for pheresis procedure and disposable pheresis bowl therefor
US4204537A (en) 1974-08-15 1980-05-27 Haemonetics Corporation Process for pheresis procedure and disposable plasma
US3982691A (en) 1974-10-09 1976-09-28 Schlutz Charles A Centrifuge separation and washing device and method
US3972812A (en) 1975-05-08 1976-08-03 Becton, Dickinson And Company Blood serum separation filter disc
US4055501A (en) 1976-01-16 1977-10-25 Sherwood Medical Industries Inc. Fluid collection device with phase partitioning means
US4027660A (en) * 1976-04-02 1977-06-07 Wardlaw Stephen C Material layer volume determination
CA1074273A (fr) * 1976-05-06 1980-03-25 Sherwood Medical Industries Inc. Dispositif pour la separation de phases
US4046699A (en) 1976-11-01 1977-09-06 Corning Glass Works Access device for centrifugal separation assemblies
DE2800934C2 (de) 1977-01-10 1986-09-18 Robert Aaron Guilford Conn. Levine Verfahren zur Durchführung von Volumenmessungen an der Zwischenschicht zwischen der Erythrozytenschicht und der Plasmaschicht einer zentrifugierten Blutprobe
US5217426A (en) 1977-08-12 1993-06-08 Baxter International Inc. Combination disposable plastic blood receiving container and blood component centrifuge
US4187979A (en) * 1978-09-21 1980-02-12 Baxter Travenol Laboratories, Inc. Method and system for fractionating a quantity of blood into the components thereof
US4303193A (en) 1979-01-22 1981-12-01 Haemonetics Corporation Apparatus for separating blood into components thereof
US4229298A (en) 1979-02-05 1980-10-21 The Western States Machine Company Method and apparatus for determining the thickness of a charge wall formed in a centrifugal basket
AT359653B (de) 1979-02-15 1980-11-25 Immuno Ag Verfahren zur herstellung eines gewebekleb- stoffes
AT359652B (de) 1979-02-15 1980-11-25 Immuno Ag Verfahren zur herstellung eines gewebekleb- stoffes
US4314823A (en) * 1979-03-05 1982-02-09 Dionex Corporation Combination apparatus and method for chromatographic separation and quantitative analysis of multiple ionic species
JPS5917386B2 (ja) 1979-03-23 1984-04-20 テルモ株式会社 血液分離方法および装置
US4300717A (en) 1979-04-02 1981-11-17 Haemonetics Corporation Rotary centrifuge seal
US4269718A (en) * 1980-05-05 1981-05-26 The Institutes Of Medical Sciences Process and device for centrifugal separation of platelets
EP0039898B1 (fr) 1980-05-08 1984-08-22 Terumo Corporation Appareil pour la séparation du sang
US4332351A (en) 1980-09-22 1982-06-01 International Business Machines Corporation Blood fraction extracting centrifuge
DE3101733C2 (de) 1981-01-21 1982-10-14 Uwe Dr.Med. 2300 Kiel Ballies Trennelement in einem Trennröhrchen zur Zentrifugaltrennung
US4424132A (en) * 1981-02-05 1984-01-03 Asahi Kasei Kogyo Kabushiki Kaisha Apparatus and method for separating blood components
DE3105624A1 (de) 1981-02-16 1982-09-02 Hormon-Chemie München GmbH, 8000 München Material zum abdichten und heilen von wunden
US4417981A (en) 1981-05-04 1983-11-29 Becton, Dickinson And Company Blood phase separator device
US4322298A (en) * 1981-06-01 1982-03-30 Advanced Blood Component Technology, Inc. Centrifugal cell separator, and method of use thereof
DE3175003D1 (en) * 1981-06-25 1986-08-28 Serapharm Gmbh & Co Kg Enriched plasma derivative for promoting wound sealing and wound healing
ATE20824T1 (de) 1981-06-25 1986-08-15 Serapharm Gmbh & Co Kg Angereichertes plasmaderivat zur unterstuetzung von wundverschluss und wundheilung.
US4442655A (en) * 1981-06-25 1984-04-17 Serapharm Michael Stroetmann Fibrinogen-containing dry preparation, manufacture and use thereof
US4735726A (en) * 1981-07-22 1988-04-05 E. I. Du Pont De Nemours And Company Plasmapheresis by reciprocatory pulsatile filtration
GB2104401B (en) * 1981-08-25 1985-03-20 Krauss Maffei Ag Support plate for centrifuges
US4464167A (en) 1981-09-03 1984-08-07 Haemonetics Corporation Pheresis apparatus
US4416654A (en) 1981-09-03 1983-11-22 Haemonetics Corporation Pheresis apparatus
DE3203775A1 (de) * 1982-02-04 1983-08-11 Behringwerke Ag, 3550 Marburg Fibrinogenzubereitung, verfahen zu ihrer herstellungund ihre verwendung
JPS5928766A (ja) 1982-08-10 1984-02-15 Sony Corp 遅延回路
US4680025A (en) * 1982-08-24 1987-07-14 Baxter Travenol Laboratories, Inc. Blood component collection systems and methods
US4722790A (en) * 1982-09-13 1988-02-02 Cawley Leo P On-line plasma modification means
US4839058A (en) 1982-09-13 1989-06-13 Cawley Leo P On-line plasma modification method
EP0109374A1 (fr) 1982-10-08 1984-05-23 Antoon Soetens Dispositif pour capter l'énergie solaire sur un toit ou analogue
SE8206767D0 (sv) 1982-11-26 1982-11-26 Seroteknik Hb Sett och anordning for satsvis centrifugalseparering av blod
US5034135A (en) 1982-12-13 1991-07-23 William F. McLaughlin Blood fractionation system and method
US4632761A (en) 1983-08-15 1986-12-30 W. R. Grace & Co. Centrifugal microconcentrator and methods for its use
US4755301A (en) 1983-08-15 1988-07-05 W. R. Grace & Co. Apparatus and method for centrifugal recovery of retentate
US4672969A (en) 1983-10-06 1987-06-16 Sonomo Corporation Laser healing method
JPS60250014A (ja) 1984-05-28 1985-12-10 Mitsui Toatsu Chem Inc 濃縮用樹脂
CH665565A5 (de) * 1983-10-22 1988-05-31 Mitsui Toatsu Chemicals Verfahren zur steuerung der konzentration einer eine makromolekulare verbindung enthaltenden waessrigen loesung oder emulsion.
CA1244774A (fr) 1983-11-09 1988-11-15 Thomas Jefferson University Milieu de conservation des plaquettes sanguines
DE3410286C2 (de) 1984-03-21 1986-01-23 Fresenius AG, 6380 Bad Homburg Verfahren zur Trennung von Blut sowie Vorrichtung zur Durchführung des Verfahrens
AT379311B (de) 1984-03-29 1985-12-27 Immuno Ag Vorrichtung zur applikation eines gewebeklebstoffes
JPS6144825A (ja) * 1984-08-09 1986-03-04 Unitika Ltd 止血剤
US4610656A (en) * 1984-08-21 1986-09-09 Mehealus Partnership Fully portable semi-automatic mechanical heart-lung substitution system and method
US4776964A (en) 1984-08-24 1988-10-11 William F. McLaughlin Closed hemapheresis system and method
US4928603A (en) * 1984-09-07 1990-05-29 The Trustees Of Columbia University In The City Of New York Method of preparing a cryoprecipitated suspension and use thereof
US4627879A (en) 1984-09-07 1986-12-09 The Trustees Of Columbia University In The City Of New York Fibrin adhesive prepared as a concentrate from single donor fresh frozen plasma
US5165938A (en) 1984-11-29 1992-11-24 Regents Of The University Of Minnesota Wound healing agents derived from platelets
US4639316A (en) * 1984-12-14 1987-01-27 Becton, Dickinson And Company Automatic liquid component separator
AT382783B (de) * 1985-06-20 1987-04-10 Immuno Ag Vorrichtung zur applikation eines gewebeklebstoffes
SE448323B (sv) 1985-08-27 1987-02-09 Ersson Nils Olof Forfarande och anordnig att separera serum eller plasma fran blod
US4846974A (en) 1985-11-14 1989-07-11 Norfolk Scientific, Inc. Centrifuge system and fluid container therefor
US4724317A (en) * 1985-12-05 1988-02-09 Baxter Travenol Laboratories, Inc. Optical data collection apparatus and method used with moving members
US4871462A (en) 1985-12-23 1989-10-03 Haemonetics Corporation Enhanced separation of blood components
US4755300A (en) 1985-12-23 1988-07-05 Haemonetics Corporation Couette membrane filtration apparatus for separating suspended components in a fluid medium using high shear
AU578554B2 (en) 1986-01-24 1988-10-27 Japanese Red Cross Society Centrifugal method of separating blood components
US5112490A (en) * 1986-02-19 1992-05-12 Jon Turpen Sample filtration, separation and dispensing device
ES2004281A6 (es) 1986-04-04 1988-12-16 Univ Jefferson Una superficie protesica implantable para implantacion en un paciente humano
SE8601891D0 (sv) 1986-04-24 1986-04-24 Svante Jonsson Maskin for plasmabytesbehandling och trombocytgivning
DE3622642A1 (de) 1986-07-05 1988-01-14 Behringwerke Ag Einkomponenten-gewebekleber sowie verfahren zu seiner herstellung
US4983158A (en) * 1986-07-22 1991-01-08 Haemonetics Corporation Plasmapheresis centrifuge bowl
US4943273A (en) 1986-07-22 1990-07-24 Haemonetics Corporation Disposable centrifuge bowl for blood processing
US4714457A (en) 1986-09-15 1987-12-22 Robert Alterbaum Method and apparatus for use in preparation of fibrinogen from a patient's blood
DK475386D0 (da) 1986-10-03 1986-10-03 Weis Fogh Ulla Sivertsen Fremgangsmaade og apparat til fremstilling af biologiske stoffer
US4929242A (en) * 1986-11-26 1990-05-29 Baxter International Inc. Solution and method for maintaining patency of a catheter
CH673117A5 (fr) * 1986-12-10 1990-02-15 Ajinomoto Kk
US4933291A (en) 1986-12-22 1990-06-12 Eastman Kodak Company Centrifugable pipette tip and pipette therefor
US5053127A (en) 1987-01-13 1991-10-01 William F. McLaughlin Continuous centrifugation system and method for directly deriving intermediate density material from a suspension
US5370802A (en) 1987-01-30 1994-12-06 Baxter International Inc. Enhanced yield platelet collection systems and methods
US4834890A (en) * 1987-01-30 1989-05-30 Baxter International Inc. Centrifugation pheresis system
US5104526A (en) * 1987-01-30 1992-04-14 Baxter International Inc. Centrifugation system having an interface detection system
US4832851A (en) * 1987-02-02 1989-05-23 W. R. Grace & Co. Centrifugal force-enhanced filtration of fluids
US4983157A (en) * 1987-03-23 1991-01-08 Ceramics Process Systems Corp. Centrifugation system using static layer
US5019243A (en) * 1987-04-03 1991-05-28 Mcewen James A Apparatus for collecting blood
US5030341A (en) 1987-04-03 1991-07-09 Andronic Technologies, Inc. Apparatus for separating phases of blood
IT1203461B (it) * 1987-04-08 1989-02-15 Dideco Spa Cella per la centrifugazione del sangue
US4738655A (en) * 1987-06-17 1988-04-19 Utah Bioresearch, Inc. Apparatus and method for obtaining a rapid hematocrit
DE3723092C1 (de) 1987-07-13 1989-01-26 Westfalia Separator Ag Durchlaufzentrifuge zur industriellen Produktion von Proteinen aus menschlichem Blutplasma
US5260420A (en) 1987-07-30 1993-11-09 Centre Regional De Transfusion Sanguine De Lille Concentrate of thrombin coagulable proteins, the method of obtaining same and therapeutical use thereof
DE3726227A1 (de) * 1987-08-07 1989-02-16 Krauss Maffei Ag Vorrichtung zum ergebnisabhaengigen steuern einer filterzentrifuge
US4877520A (en) 1987-10-08 1989-10-31 Becton, Dickinson And Company Device for separating the components of a liquid sample having higher and lower specific gravities
US4818386A (en) * 1987-10-08 1989-04-04 Becton, Dickinson And Company Device for separating the components of a liquid sample having higher and lower specific gravities
US4844818A (en) 1987-10-23 1989-07-04 Becton Dickinson & Company Method for separating the cellular components of blood samples
US4957638A (en) 1987-10-23 1990-09-18 Becton Dickinson And Company Method for separating the cellular components of blood samples
US5290552A (en) * 1988-05-02 1994-03-01 Matrix Pharmaceutical, Inc./Project Hear Surgical adhesive material
DE68902698C5 (de) * 1988-06-23 2005-07-14 Asahi Medical Co. Ltd. Verfahren zur Trennung von Blut in Blutkomponenten und Einheit zur Trennung von Blutkomponenten.
US4874368A (en) 1988-07-25 1989-10-17 Micromedics, Inc. Fibrin glue delivery system
US4846780A (en) 1988-08-10 1989-07-11 Exxon Production Research Company Centrifuge processor and liquid level control system
US4902281A (en) * 1988-08-16 1990-02-20 Corus Medical Corporation Fibrinogen dispensing kit
US4946601A (en) 1988-08-22 1990-08-07 Sherwood Medical Company Blood serum separator tube
US5002571A (en) * 1989-02-06 1991-03-26 Donnell Jr Francis E O Intraocular lens implant and method of locating and adhering within the posterior chamber
SE8900586L (sv) 1989-02-21 1990-08-22 Pharmacia Ab Komposition och foerfarande foer att foerhindra adhesion mellan kroppsvaevnader
US5226877A (en) 1989-06-23 1993-07-13 Epstein Gordon H Method and apparatus for preparing fibrinogen adhesive from whole blood
US5000970A (en) * 1989-06-30 1991-03-19 Horizons International Foods, Inc. Process for preparing reheatable french fried potatoes
US5258126A (en) 1989-09-12 1993-11-02 Pall Corporation Method for obtaining platelets
US5316674A (en) 1989-09-12 1994-05-31 Pall Corporation Device for processing blood for human transfusion
US5100564A (en) * 1990-11-06 1992-03-31 Pall Corporation Blood collection and processing system
US5152905A (en) 1989-09-12 1992-10-06 Pall Corporation Method for processing blood for human transfusion
IL95641A0 (en) 1989-09-15 1991-06-30 Curative Tech Inc Preparation of a platelet releasate product
US5104375A (en) 1989-10-16 1992-04-14 Johnson & Johnson Medical, Inc. Locking holder for a pair of syringes and method of use
US5318524A (en) 1990-01-03 1994-06-07 Cryolife, Inc. Fibrin sealant delivery kit
US5030215A (en) 1990-01-03 1991-07-09 Cryolife, Inc. Preparation of fibrinogen/factor XIII precipitate
US5219328A (en) 1990-01-03 1993-06-15 Cryolife, Inc. Fibrin sealant delivery method
NL9000090A (nl) 1990-01-15 1991-08-01 Harimex Ligos Bv Werkwijze voor het bereiden van een fibrinogeenconcentraat uit bloedplasma, inrichting voor het uitvoeren van deze werkwijze en werkwijze voor het bereiden van fibrinogeen uit het concentraat.
US5185001A (en) * 1990-01-18 1993-02-09 The Research Foundation Of State University Of New York Method of preparing autologous plasma fibrin and application apparatus therefor
US5045048A (en) 1990-03-29 1991-09-03 Haemonetics Corporation Rotary centrifuge bowl and seal for blood processing
US5204537A (en) 1990-03-30 1993-04-20 Recognition Equipment Incorporated Thickness sensor comprising a leaf spring means, and a light sensor
DK119490D0 (da) 1990-05-14 1990-05-14 Unes As Apparat til fremstilling af et koncentrat af koagulationsfaktorer, saasom fibrinogen, fra en blodportion
US5171456A (en) 1990-06-14 1992-12-15 Baxter International Inc. Automated blood component separation procedure and apparatus promoting different functional characteristics in multiple blood components
US5156586A (en) * 1990-07-10 1992-10-20 Bardyne Orbital separator for orbitally separating a mixture
FR2665378B1 (fr) * 1990-08-03 1992-10-09 Guigan Jean Dispositif pour separer par centrifugation deux phases d'un echantillon d'un liquide heterogene, utilisable notamment pour la separation du plasma du sang total.
US5420250A (en) 1990-08-06 1995-05-30 Fibrin Corporation Phase transfer process for producing native plasma protein concentrates
US5641622A (en) * 1990-09-13 1997-06-24 Baxter International Inc. Continuous centrifugation process for the separation of biological components from heterogeneous cell populations
US5173295A (en) * 1990-10-05 1992-12-22 Advance Biofactures Of Curacao, N.V. Method of enhancing the regeneration of injured nerves and adhesive pharamaceutical formulation therefor
US5112484A (en) * 1990-10-11 1992-05-12 Zuk, Inc. Filtration apparatus
US5217627A (en) 1990-11-06 1993-06-08 Pall Corporation System and method for processing biological fluid
US6054122A (en) * 1990-11-27 2000-04-25 The American National Red Cross Supplemented and unsupplemented tissue sealants, methods of their production and use
US6197325B1 (en) * 1990-11-27 2001-03-06 The American National Red Cross Supplemented and unsupplemented tissue sealants, methods of their production and use
US6117425A (en) 1990-11-27 2000-09-12 The American National Red Cross Supplemented and unsupplemented tissue sealants, method of their production and use
US5234608A (en) 1990-12-11 1993-08-10 Baxter International Inc. Systems and methods for processing cellular rich suspensions
JPH0774772B2 (ja) * 1990-12-31 1995-08-09 エイ． レビン ロバート 血液サンプリング組立体、ターゲット細胞の採取方法およびターゲット成分の採取方法
US5137832A (en) 1991-01-02 1992-08-11 Becton Dickinson & Company Quantification of fibrinogen in whole blood samples contained in a tube using a float to separate materials
US5206023A (en) * 1991-01-31 1993-04-27 Robert F. Shaw Method and compositions for the treatment and repair of defects or lesions in cartilage
US5156613A (en) 1991-02-13 1992-10-20 Interface Biomedical Laboratories Corp. Collagen welding rod material for use in tissue welding
US5269927A (en) 1991-05-29 1993-12-14 Sherwood Medical Company Separation device for use in blood collection tubes
US5236604A (en) 1991-05-29 1993-08-17 Sherwood Medical Company Serum separation blood collection tube and the method of using thereof
FR2679251B1 (fr) * 1991-07-18 1993-11-12 Nord Assoc Essor Transfusion San Procede de preparation d'un concentre de thrombine humaine destine a un usage therapeutique.
DE4126341C1 (fr) * 1991-08-09 1993-01-28 Fresenius Ag, 6380 Bad Homburg, De
BR9103724A (pt) 1991-08-29 1993-03-30 Univ Estadual Paulista Julio D Cola de fibrina derivada de veneno de cobra e processo para sua preparacao
DE4129516C2 (de) * 1991-09-06 2000-03-09 Fresenius Ag Verfahren und Vorrichtung zum Trennen von Blut in seine Bestandteile
EP0534178B1 (fr) 1991-09-27 2001-04-18 Omrix Biopharmaceuticals S.A. Colle améliorée pour tissus préparée à partir de cryoprécipité
US5344752A (en) 1991-10-30 1994-09-06 Thomas Jefferson University Plasma-based platelet concentrate preparations
CA2074671A1 (fr) * 1991-11-04 1993-05-05 Thomas Bormann Dispositif et methode permettant de separer le plasma d'un liquide biologique
US5190057A (en) * 1991-12-13 1993-03-02 Faezeh Sarfarazi Sarfarazi method of closing a corneal incision
DE4202667C1 (fr) 1992-01-29 1993-05-13 Behringwerke Ag, 3550 Marburg, De
DE4204012A1 (de) 1992-02-12 1993-08-19 Ulrich Prof Dr Zimmermann Mitogenfreie substanz, deren herstellung und verwendung
US5298016A (en) 1992-03-02 1994-03-29 Advanced Haemotechnologies Apparatus for separating plasma and other wastes from blood
US5271852A (en) 1992-05-01 1993-12-21 E. I. Du Pont De Nemours And Company Centrifugal methods using a phase-separation tube
US5403272A (en) * 1992-05-29 1995-04-04 Baxter International Inc. Apparatus and methods for generating leukocyte free platelet concentrate
DK83092D0 (da) 1992-06-24 1992-06-24 Unes As Fremgangsmaade til udvinding af thrombin
US5443481A (en) 1992-07-27 1995-08-22 Lee; Benjamin I. Methods and device for percutaneous sealing of arterial puncture sites
WO1994006460A1 (fr) 1992-09-21 1994-03-31 Vitaphore Corporation Bouchons d'embolisation pour vaisseaux sanguins
FR2696095B1 (fr) 1992-09-30 1994-11-25 Inoteb Colle biologique à base de protéines coagulables par la thrombine, enrichie en facteurs plaquettaires, sa préparation et son application.
CN1091315A (zh) 1992-10-08 1994-08-31 E·R·斯奎布父子公司 血纤维蛋白封闭剂组合物及其使用方法
WO1994012223A1 (fr) * 1992-12-01 1994-06-09 Haemonetics Corporation Appareil et procede de pherese des globules rouges
US5290918A (en) * 1993-02-23 1994-03-01 Haemacure Biotech Inc. Process for the obtention of a biological adhesive made of concentrated coagulation factors by acidic precipitation
US5395923A (en) 1993-02-23 1995-03-07 Haemacure-Biotech, Inc. Process for the obtention of a biological adhesive made of concentrated coagulation factors by "salting-out"
JP3210759B2 (ja) 1993-02-25 2001-09-17 昭和電工株式会社 液晶カラーフィルター
US5330974A (en) 1993-03-01 1994-07-19 Fibratek, Inc. Therapeutic fibrinogen compositions
US5614106A (en) * 1993-03-12 1997-03-25 Baxter International Inc. Method and apparatus for collection of platelets
WO1994025086A1 (fr) * 1993-04-27 1994-11-10 Haemonetics Corporation Appareil et procede de pherese
US5456885A (en) 1993-07-12 1995-10-10 Coleman; Charles M. Fluid collection, separation and dispensing tube
CA2131738C (fr) 1993-11-17 2001-09-04 Lonny R. Kelley Separateur centrifuge
ZA948564B (en) 1993-11-19 1995-07-26 Bristol Myers Squibb Co Liquid separation apparatus and method
US5411885A (en) 1993-12-17 1995-05-02 New York Blood Center, Inc. Methods for tissue embedding and tissue culturing
US5437598A (en) * 1994-01-21 1995-08-01 Cobe Laboratories, Inc. Automation of plasma sequestration
JP4011106B2 (ja) 1994-02-23 2007-11-21 協和醗酵工業株式会社 血小板増殖促進剤
US5533518A (en) 1994-04-22 1996-07-09 Becton, Dickinson And Company Blood collection assembly including mechanical phase separating insert
US5577513A (en) 1994-08-31 1996-11-26 Activated Cell Therapy, Inc. Centrifugation syringe, system and method
US5733253A (en) * 1994-10-13 1998-03-31 Transfusion Technologies Corporation Fluid separation system
US5733446A (en) 1994-12-02 1998-03-31 Bristol-Myers Squibb Company Centrifuge with annular filter
CA2182862C (fr) 1994-12-07 2006-10-03 Richard D. Antanavich Compositions de concentre de plasma et d'agent de scellement tissulaire
US5560830A (en) 1994-12-13 1996-10-01 Coleman; Charles M. Separator float and tubular body for blood collection and separation and method of use thereof
NO301562B1 (no) * 1994-12-21 1997-11-10 Exxon Production Research Co Anordning for måling
US5510102A (en) * 1995-01-23 1996-04-23 The Regents Of The University Of California Plasma and polymer containing surgical hemostatic adhesives
US6676629B2 (en) * 1995-02-06 2004-01-13 Mark S. Andrew Tissue liquefaction and aspiration for dental treatment
US7011644B1 (en) * 1995-02-06 2006-03-14 Andrew Mark S Tissue liquefaction and aspiration for dental treatment
FR2731176B1 (fr) 1995-03-02 1997-04-30 Sidel Sa Installation de fabrication de recipients par soufflage de preformes en matiere plastique
US5733545A (en) * 1995-03-03 1998-03-31 Quantic Biomedical Partners Platelet glue wound sealant
US5643192A (en) 1995-04-06 1997-07-01 Hamilton Civic Hospitals Research Development, Inc. Autologous fibrin glue and methods for its preparation and use
US6053856A (en) * 1995-04-18 2000-04-25 Cobe Laboratories Tubing set apparatus and method for separation of fluid components
US6022306A (en) * 1995-04-18 2000-02-08 Cobe Laboratories, Inc. Method and apparatus for collecting hyperconcentrated platelets
US5674173A (en) 1995-04-18 1997-10-07 Cobe Laboratories, Inc. Apparatus for separating particles
JPH11508813A (ja) * 1995-06-06 1999-08-03 クウォンティック バイオメディカル パートナーズ 血漿を濃縮するための装置及び方法
US5738644A (en) * 1995-06-07 1998-04-14 Cobe Laboratories, Inc. Extracorporeal blood processing methods and apparatus
US5632905A (en) 1995-08-07 1997-05-27 Haynes; John L. Method and apparatus for separating formed and unformed components
EP0794835A1 (fr) 1995-10-03 1997-09-17 Beckman Instruments, Inc. Systeme et procede de separation du sang par rotation axiale
US5736033A (en) * 1995-12-13 1998-04-07 Coleman; Charles M. Separator float for blood collection tubes with water swellable material
US5643193A (en) 1995-12-13 1997-07-01 Haemonetics Corporation Apparatus for collection washing and reinfusion of shed blood
US6025201A (en) * 1995-12-28 2000-02-15 Bayer Corporation Highly sensitive, accurate, and precise automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples
US5889984A (en) * 1996-08-19 1999-03-30 Intel Corporation Floating point and integer condition compatibility for conditional branches and conditional moves
US5889584A (en) 1997-03-10 1999-03-30 Robert A. Levine Assembly for rapid measurement of cell layers
WO1998043720A1 (fr) 1997-04-03 1998-10-08 Baxter International Inc. Systemes et procedes de detection et de regulation d'une interface
WO1998048938A1 (fr) * 1997-04-25 1998-11-05 Washington State University Research Foundation Separateur des globules sanguins, centrifuge, semi-continu et de faible volume
US5860937A (en) * 1997-04-30 1999-01-19 Becton, Dickinson & Company Evacuated sample collection tube with aqueous additive
WO1998057678A2 (fr) 1997-06-18 1998-12-23 Cohesion Technologies, Inc. Compositions contenant de la thrombine et du collagene microfibrillaire; procede de preparation et d'utilisation de ces compositions
IT1292410B1 (it) * 1997-06-24 1999-02-08 Roberto Beretta Contenitore di pronto impiego per ottenere colla di fibrina autologa
US6979307B2 (en) 1997-06-24 2005-12-27 Cascade Medical Enterprises Llc Systems and methods for preparing autologous fibrin glue
US5918622A (en) 1997-07-01 1999-07-06 Bermad Separation valve
US6200287B1 (en) * 1997-09-05 2001-03-13 Gambro, Inc. Extracorporeal blood processing methods and apparatus
US5934803A (en) * 1997-10-30 1999-08-10 Physical Systems, Inc. Apparatus and method for mixing multi-part reaction materials under vacuum
US6051146A (en) * 1998-01-20 2000-04-18 Cobe Laboratories, Inc. Methods for separation of particles
US5924972A (en) 1998-03-24 1999-07-20 Turvaville; L. Jackson Portable D.C. powered centrifuge
JP2000117150A (ja) * 1998-10-16 2000-04-25 Kdk Corp 遠心分離方法及びそれに用いる遠心容器
US6563953B2 (en) 1998-11-30 2003-05-13 Microsoft Corporation Predictive image compression using a single variable length code for both the luminance and chrominance blocks for each macroblock
US6280400B1 (en) 1998-12-05 2001-08-28 Becton Dickinson And Company Device and method for separating component of a liquid sample
US6516953B1 (en) 1998-12-05 2003-02-11 Becton, Dickinson And Company Device for separating components of a fluid sample
US6153113A (en) 1999-02-22 2000-11-28 Cobe Laboratories, Inc. Method for using ligands in particle separation
US6322785B1 (en) 1999-03-02 2001-11-27 Natrex Technologies Methods and compositions for bone graft implants
US20050153442A1 (en) 1999-03-10 2005-07-14 Adam Katz Adipose-derived stem cells and lattices
US20030082152A1 (en) 1999-03-10 2003-05-01 Hedrick Marc H. Adipose-derived stem cells and lattices
KR100979664B1 (ko) 1999-03-10 2010-09-02 유니버시티 오브 피츠버그 오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 지방 유래 간세포 및 격자
US6777231B1 (en) 1999-03-10 2004-08-17 The Regents Of The University Of California Adipose-derived stem cells and lattices
US20050076396A1 (en) * 1999-03-10 2005-04-07 Katz Adam J. Adipose-derived stem cells and lattices
US6334842B1 (en) * 1999-03-16 2002-01-01 Gambro, Inc. Centrifugal separation apparatus and method for separating fluid components
US6296602B1 (en) 1999-03-17 2001-10-02 Transfusion Technologies Corporation Method for collecting platelets and other blood components from whole blood
US6629919B2 (en) 1999-06-03 2003-10-07 Haemonetics Corporation Core for blood processing apparatus
EP1057534A1 (fr) * 1999-06-03 2000-12-06 Haemonetics Corporation Bol de centrifugation avec noyau filtrant
US6472162B1 (en) 1999-06-04 2002-10-29 Thermogenesis Corp. Method for preparing thrombin for use in a biological glue
US6316247B1 (en) 1999-06-15 2001-11-13 University Of Pittsburgh System and method for refining liposuctioned adipose tissue
US6758978B1 (en) 1999-08-06 2004-07-06 Gl&V Management Hungary Kft. Deep bed thickener/clarifiers with enhanced liquid removal
US6860846B2 (en) * 1999-09-03 2005-03-01 Baxter International Inc. Blood processing systems and methods with umbilicus-driven blood processing chambers
ATE382408T1 (de) 2000-04-28 2008-01-15 Harvest Technologies Corp Plattentrenneinrichtung für blutbestandteile
US6764531B2 (en) 2001-07-06 2004-07-20 J. S. Hogan Method and apparatus for cleaning a gas
US20030054331A1 (en) * 2001-09-14 2003-03-20 Stemsource, Inc. Preservation of non embryonic cells from non hematopoietic tissues
KR100930139B1 (ko) 2001-12-07 2009-12-07 사이토리 테라퓨틱스, 인크. 처리된 리포애스퍼레이트 세포로 환자를 치료하기 위한시스템 및 방법
US7585670B2 (en) 2001-12-07 2009-09-08 Cytori Therapeutics, Inc. Automated methods for isolating and using clinically safe adipose derived regenerative cells
US9597395B2 (en) * 2001-12-07 2017-03-21 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions
US7514075B2 (en) * 2001-12-07 2009-04-07 Cytori Therapeutics, Inc. Systems and methods for separating and concentrating adipose derived stem cells from tissue
EP1467746A4 (fr) * 2001-12-20 2006-10-04 Macropore Inc Systemes et procedes permettant de traiter des patients avec un materiau riche en collagene extrait de tissus adipeux
AU2003247350A1 (en) * 2002-04-24 2003-11-10 Interpore Orthopaedics, Inc. Blood separation ande concentration system
US6905612B2 (en) 2003-03-21 2005-06-14 Hanuman Llc Plasma concentrate apparatus and method
US7374678B2 (en) 2002-05-24 2008-05-20 Biomet Biologics, Inc. Apparatus and method for separating and concentrating fluids containing multiple components
US20030205538A1 (en) 2002-05-03 2003-11-06 Randel Dorian Methods and apparatus for isolating platelets from blood
US20040182795A1 (en) * 2003-03-21 2004-09-23 Randel Dorian Apparatus and method for concentration of plasma from whole blood
WO2003099412A1 (fr) * 2002-05-24 2003-12-04 Biomet Manufacturing Corp. Appareil et procede pour separer et concentrer des fluides contenant de multiples composants
US7845499B2 (en) 2002-05-24 2010-12-07 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
WO2004009207A1 (fr) 2002-07-18 2004-01-29 Hanuman Llc Appareil et procede de concentration plasmatique
EP1549552B1 (fr) 2002-09-19 2013-06-26 Harvest Technologies Corporation Unite sterile jetable
US7445125B2 (en) 2003-05-19 2008-11-04 Harvest Technologies Corporation Method and apparatus for separating fluid components
ES2633604T3 (es) * 2003-09-17 2017-09-22 Cytori Therapeutics, Inc. Métodos para usar células regenerativas derivadas de adiposo en el tratamiento de una enfermedad vascular periférica
EP1688183A1 (fr) 2005-02-03 2006-08-09 Jean-Denis Rochat Procede et dispositif jetable pour la separation par centrifugation d'un liquide physiologique
US7708152B2 (en) 2005-02-07 2010-05-04 Hanuman Llc Method and apparatus for preparing platelet rich plasma and concentrates thereof
US7866485B2 (en) * 2005-02-07 2011-01-11 Hanuman, Llc Apparatus and method for preparing platelet rich plasma and concentrates thereof
ES2426941T3 (es) 2005-02-07 2013-10-25 Hanuman Llc Aparato y procedimiento de concentrados de plasma rico en plaquetas
US7766900B2 (en) 2005-02-21 2010-08-03 Biomet Manufacturing Corp. Method and apparatus for application of a fluid
US7694828B2 (en) 2005-04-27 2010-04-13 Biomet Manufacturing Corp. Method and apparatus for producing autologous clotting components
US7771590B2 (en) * 2005-08-23 2010-08-10 Biomet Manufacturing Corp. Method and apparatus for collecting biological materials
JP5479319B2 (ja) 2007-04-12 2014-04-23 バイオメット・バイオロジックス・リミテッド・ライアビリティ・カンパニー ブイ式懸濁液分画システム
US8012077B2 (en) 2008-05-23 2011-09-06 Biomet Biologics, Llc Blood separating device
US8187475B2 (en) 2009-03-06 2012-05-29 Biomet Biologics, Llc Method and apparatus for producing autologous thrombin

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585007A (en) 1994-12-07 1996-12-17 Plasmaseal Corporation Plasma concentrate and tissue sealant methods and apparatuses for making concentrated plasma and/or tissue sealant

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JP4974902B2 (ja) 2012-07-11
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JP2012030085A (ja) 2012-02-16
JP5523418B2 (ja) 2014-06-18
EP1848472A1 (fr) 2007-10-31
EP2666493A3 (fr) 2014-04-30
WO2006086199A1 (fr) 2006-08-17
US20060175244A1 (en) 2006-08-10
US20110042296A1 (en) 2011-02-24
EP2666494A3 (fr) 2014-04-23
JP4961354B2 (ja) 2012-06-27
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US7824559B2 (en) 2010-11-02
ES2426941T3 (es) 2013-10-25
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JP5248665B2 (ja) 2013-07-31
EP2666494A2 (fr) 2013-11-27
JP2008535531A (ja) 2008-09-04
EP2666493A2 (fr) 2013-11-27
US8096422B2 (en) 2012-01-17
EP2910258B1 (fr) 2018-08-01
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