EP2941480B2 - Incubateur - Google Patents

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Publication number
EP2941480B2
EP2941480B2 EP13810891.5A EP13810891A EP2941480B2 EP 2941480 B2 EP2941480 B2 EP 2941480B2 EP 13810891 A EP13810891 A EP 13810891A EP 2941480 B2 EP2941480 B2 EP 2941480B2
Authority
EP
European Patent Office
Prior art keywords
sample chamber
incubator
sample
loading
access opening
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP13810891.5A
Other languages
German (de)
English (en)
Other versions
EP2941480A1 (fr
EP2941480B1 (fr
Inventor
Michael MOMBOISSE
Christian George
Volker Lob
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INHECO INDUSTRIAL HEATING AND COOLING GmbH
Original Assignee
Inheco Industrial Heating And Cooling GmbH
INHECO Ind HEATING AND COOLING GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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Application filed by Inheco Industrial Heating And Cooling GmbH, INHECO Ind HEATING AND COOLING GmbH filed Critical Inheco Industrial Heating And Cooling GmbH
Publication of EP2941480A1 publication Critical patent/EP2941480A1/fr
Publication of EP2941480B1 publication Critical patent/EP2941480B1/fr
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control

Definitions

  • the present invention relates to an incubator for taking samples taken in sample containers with a specific predetermined temperature comprising a temperature-controllable sample chamber with at least one closable access opening and at least one input and export mechanism carrying a sample container carrier, with which the sample container carrier carrying a sample container is passed through an access opening of the sample container Incubators can be moved into the sample chamber and moved out of this.
  • Incubators of the aforementioned type are known from the prior art, for example from the DE 10 2005 036 763 A1 , well known.
  • a drawer carrying the sample container and a corresponding drawer mechanism are provided, with which the sample container can be moved into the sample chamber through an access opening of the incubator, which can be closed by means of a lid, and moved out of the sample chamber again after an incubation procedure has been carried out.
  • the drawer mechanism carrying the drawer and the lid is at least partially arranged and mounted inside the sample chamber.
  • the drawer mechanism of such an incubator is often exposed to the potentially harmful atmosphere in the sample chamber, in particular by within the incubator if necessary.
  • Partially evaporating chemical or biological samples or externally introduced into the sample chamber Gases is contaminated, which makes a regular cleaning of the drawer mechanism required and the other - eg due to thereby promoted corrosion processes - detrimental to the life of a corresponding drawer mechanism.
  • the sample chamber as such is difficult and cumbersome to clean because of the drawer mechanism arranged or mounted at least partially therein, which also proves to be disadvantageous.
  • the at least one input and export mechanism is completely mounted outside the sample chamber and carries the retracted into the sample chamber Proben inviteniscle even with a closed access opening.
  • an incubator with an input and export mechanism for loading and unloading the sample chamber is provided with the samples to be tempered in suitable sample containers, in which the outside of the sample chamber mounted, ie outside the sample chamber or stored supported input and export mechanism carries the sample container located within the sample chamber even when the access opening is closed.
  • the entire support (ie the suspension or storage) of the input and export mechanism of the incubator outside of the sample chamber is arranged, so by at least one sample chamber limiting the sample chamber wall, which is advantageously fixedly mounted inside the incubator, sealed off from the interior of the sample chamber.
  • the input and export mechanism is not within the sample chamber, for example, mounted on a sample chamber wall defining the sample chamber, which also significantly simplifies the cleaning of the sample chamber because of a better accessibility so far, and also a more reliable sterility of the incubator can be achieved.
  • an easy to handle and compact design feasible input and export mechanism is provided, which permanently during the incubation process within the sample chamber Proben experiencenisvic, ie during the incubation, wearing, thus no settling or pulling out of Proben artnislvess is necessary before closing the access opening of the sample chamber.
  • sample containers and correspondingly adapted or adaptable sample container carriers can be used, wherein - in the sense of the particularly preferred use of standardized sample containers - in particular so-called microtiter plates (in English also "microwell plates” or “microplates”). called) and adapted thereto sample containers are used.
  • Microtiter plates are essentially rectangular sample containers, usually made of plastic, with a plurality of separate wells arranged in rows and columns for receiving samples which are either fully supported by the sample container carrier adapted thereto or at least kept in a peripheral area.
  • Typical microtiter plates which can be used advantageously in the context of the present invention, have e.g. 6, 12, 24, 48, 96, 384 or 1536 individual wells for receiving biological and / or chemical samples which are introduced into the respective wells by means of suitable pipettes (for example using a pipetting robot with multichannel pipettes).
  • suitable pipettes for example using a pipetting robot with multichannel pipettes.
  • an incubator according to the invention can also be adapted to the use of so-called "deep-well microplates", ie microtiter plates with comparatively deep cavities.
  • the sample chamber of an incubator according to the invention is suitably tempered, e.g. heating and / or cooling elements in the form of heating foils, Peltier and / or PTC elements, which, as is known from the prior art, to achieve as homogeneous a temperature as possible within the sample chamber at a suitable location within the chamber
  • Incubators are to be arranged, eg at the back of the sample chamber not facing the interior of at least one sample chamber wall bounding the sample chamber.
  • corresponding heating / cooling elements do not have to be directly adjacent to a sample chamber wall, but may advantageously be e.g. Also by means of suitable (eg flat design) heat pipes (so-called. "Vapor Chamber”) to achieve a unifmormen possible temperature of the at least one sample chamber wall are brought into thermally conductive contact with a specialist also apparent the geometry and the material of the sample chamber in customary manner designed or selects suitable.
  • an incubator according to the invention may, in addition to suitable means for controlling the temperature of the sample chamber, also comprise further means for the targeted setting of further ambient conditions within the sample chamber, e.g. Means for adjusting the humidity, means for supplying one or more different gases, means for exposing the samples to electromagnetic radiation (e.g., infrared or ultraviolet light, microwaves, etc.), to name just a few examples.
  • suitable means for controlling the temperature of the sample chamber also comprise further means for the targeted setting of further ambient conditions within the sample chamber, e.g. Means for adjusting the humidity, means for supplying one or more different gases, means for exposing the samples to electromagnetic radiation (e.g., infrared or ultraviolet light, microwaves, etc.), to name just a few examples.
  • electromagnetic radiation e.g., infrared or ultraviolet light, microwaves, etc.
  • a first preferred embodiment of an incubator according to the invention provides that the input and export mechanism not only completely outside the sample chamber mounted, but - even when the sample chamber is closed with befindlichem within the sample chamber Proben electniscle - is located completely outside the sample chamber.
  • the sample container carrier which is arranged within the sample chamber when the access opening is closed, can be suitably - e.g. by means of a suitable holder, which if necessary.
  • a the access opening to the sample chamber occluding lid partially penetrates - is carried by the arranged outside the sample chamber input and export mechanism, while ensuring the necessary (thermal) closure of the sample chamber.
  • the at least one access opening is closed by means of a lid, wherein the lid is advantageously heated and / or cooled.
  • a particularly homogeneous temperature distribution of the sample chamber which in turn can be tempered, can be effectively prevented, wherein in particular the heatability of the cover can also effectively prevent an otherwise possible formation of condensation on the inside of the lid closing the access opening to the interior of the sample chamber.
  • the at least one cover for closing an access opening is arranged on the at least one input and export mechanism such that the cover closes the access opening when the sample container carrier is completely retracted into the sample chamber.
  • the storage and retrieval mechanism of an incubator according to the invention which is mounted or arranged outside the sample chamber, advantageously not only carries the sample container carrier which can be moved in and out through the access opening of the sample chamber, but at the same time also the lid, with which said access opening is closed in an air-tight and gas-tight manner becomes.
  • the at least one input and export mechanism with two guides is designed like a drawer, wherein the two guides on the left and right sides each outside the sample chamber laterally delimiting wall are arranged at approximately the same height and wherein the Proben excniscle to a cross member is attached, which connects two guided in the two guides rails of the input and export mechanism together.
  • the cross member is advantageously mounted in an end region of the two laterally next to the sample chamber from the incubator out of the incubator and out retractable rails and advantageously always oriented horizontally and parallel to a housing front of the incubator. It can thus advantageously - including the cover attached thereto and the sample container carrier held thereon - be moved between a first position releasing the access opening and a second position closing the access opening by means of the lid. In the first position, the sample container carrier should evidently be moved out of the sample chamber of the incubator so far that it can be equipped with a sample container or a sample container can be removed from it, either manually or by means of an external robot system.
  • the sample container carrier In the second position, the sample container carrier is fully retracted into the sample chamber, while the cover also mounted on the cross member closes the access opening.
  • a holder connecting the sample container carrier to the cross member can be at least partially or completely projected through the cover closing the access opening or, in turn, fastened to the side of the lid facing the sample chamber (and thus indirectly to the cross member of the input and export mechanism) ,
  • the entire input and export mechanism is to be regarded as completely outside the sample chamber, since the Proben electniscle, the Proben artnis redesign the cross member mounting bracket and the lid in the sense of the invention not as part the actual import and export mechanism are to be considered, even if said components would if necessary be designed integrally with the other components of the import and export mechanism.
  • the input and export mechanism is formed in this embodiment of the invention rather by the advantageously arranged in the incubator guides, the rails guided therein and the rails connecting the cross member.
  • the cross member of the drawer-like input and export mechanism of an incubator according to the invention of the type described above is advantageously made of a material as low as possible thermal conductivity or, more preferably, at least partially coated with a (thermal) insulation.
  • the (at least one) input and export mechanism at least) one e.g. electronically controllable drive unit has or driven by this, wherein the drive unit can be advantageously formed by an electric motor.
  • Such a drive unit can then be arranged in a preferred embodiment of the present invention (also) completely outside the sample chamber, whereby on the one hand not the possibly. Dangerous atmosphere in the sample chamber and on the other hand not directly exposed to the possibly. High temperature in the sample chamber.
  • sample chamber is bounded or encapsulated on five sides by at least one sample chamber wall fixedly arranged within a housing of the incubator and on the sixth side by a closure cover having the at least one closable access opening.
  • sample chamber is e.g. bottom side, ceiling side, back, left and right sides, limited by firmly mounted inside the incubator sample chamber walls, wherein a part or all of the adjacent sample chamber walls can be integrally configured from a suitably shaped or suitably formed component.
  • Sample chamber walls which are not designed in one piece with adjacent sample chamber walls are advantageously welded to adjacent (side) walls, molded or at least suitably sealed against them.
  • the sixth side of the sample chamber which is advantageously the front of the incubator and in which the at least one access opening to the sample chamber is formed, is advantageously provided by a - e.g.
  • the end cover mounted on the outer housing of the incubator, is suitably sealed against its adjacent sample chamber walls.
  • the at least one access opening formed in the end cap is suitably dimensioned for loading and unloading purposes of the sample chamber and by means of a separate lid, e.g. can be mounted on the input and export mechanism, lockable.
  • an incubator according to the invention can then be provided in a further preferred manner that the at least one - the sample chamber to five sides limiting - sample chamber wall except possibly provided therein openings for supplying gases into the sample chamber and / or to carry out from sensors in the sample chamber has no further openings and / or no attached to the sample chamber wall or through the sample chamber wall mounted attachments.
  • this provides an incubator with a sample chamber that is particularly easy to clean.
  • the at least one sample chamber wall has a smooth course on its inner side delimiting the sample chamber with curves between the different sides of the sample chamber, which allows a further simpler and more reliable cleaning of the sample chamber.
  • the at least one input and export mechanism, the sample container carriers carried by the at least one input and export mechanism, and the sample container are in direct contact with at least one sample chamber wall in any position of the input and export mechanism ,
  • the sample container carrier is always floating from the input and export mechanism, i. without contact to the fixedly installed in the incubator walls in the sample chamber, worn, which in particular a plurality of sample containers simultaneously (and optionally. One above the other) can be tempered in one and the same sample chamber, without the need for special installations in the sample chamber.
  • the sample chamber of the incubator has a plurality of access openings and a plurality of respectively one access opening associated with input and export mechanisms, so that one and the same sample chamber of the incubator for simultaneous temperature control of the samples in different Proben thereinissen serve can, for this purpose each input and export mechanism advantageously carries exactly a sample container and this in the sense of the invention - together with the respectively on it or carried sample container - through the respective access opening into the sample chamber and can extend out of the sample chamber.
  • Proben meetingniscle differ and are adapted to different types of sample containers, but this is not mandatory.
  • such an incubator with a plurality of access openings and their associated input and export mechanisms (for loading and unloading of samples through the respective access opening) provided that the access openings of the sample chamber in a sample chamber to one side are formed towards the end cap limiting, wherein the incubator at least two replaceable end caps are associated with a different number of access openings and / or a different size of the access openings.
  • a first of two interchangeable end caps having a first plurality (e.g., two, four, six, etc.) at access ports for simultaneously loading the sample chamber of the incubator with said plurality of common "microplates", while a second (replacement) end cap e.g. a smaller number (e.g., one, two, three, etc.) at larger than the access openings in the first end cover access openings for loading the incubator with respect to conventional "microplates" significantly higher "deep-well microplates” has.
  • the cover (s) closing the respective access openings may then be necessary, if necessary, for the cover (s) closing the respective access openings to be adaptable to the different sized access openings or be exchangeable by suitably dimensioned (replacement) covers.
  • a set of an incubator according to the invention with two different (replacement) end caps and, if necessary, different (replacement) covers can be provided for closing the access openings, which may be of different sizes, in the different end caps, thus making the inventive incubator is easily adaptable to different requirement profiles.
  • an incubator 1 serves for the simultaneous admission of recorded in a total of four sample containers 2 samples with a specific predetermined temperature.
  • the incubator 1 has a temperature-controllable sample chamber 3, which is accessible from a front side F of the incubator 1 through a total of four closable access openings 4.
  • the access openings 4 are formed in a the sample chamber 3 to the front side F towards limiting end plate 5 and are each sealed by means suitably adapted thereto cover 6.
  • the incubator 1 further comprises a total of four - drawer-like design - import and export mechanisms 7, each carrying a Proben therenis redesign 8 and with which the Proben thereniscle 8 retracted by the respective input and export mechanism 7 associated access opening 4 in the sample chamber 3 and from this can be moved out.
  • Each sample container carrier 8 is in turn adapted to each receive or carry a sample container 2.
  • Each of the present total of four input and export mechanisms 7 is mounted completely outside the sample chamber 3 and arranged completely outside the sample chamber 3 and includes two each - at the same height of the incubator 1 - arranged guides 10, the left and right sides outside of a sample chamber. 3 laterally delimiting wall 21, 22 (see Fig. 4 ) are arranged.
  • a rail 11 is guided according to double arrow L linearly movable, wherein the two rails 11 of the respective input and export mechanism 7 in a protruding from the outer housing 9 of the incubator 1 end portion of the respective rail 11 by means of a cross member 12 stably connected together are.
  • both the respective access opening 4 in the end cover 5 occlusive lid 6 and the extending from the cross member 12 in the direction of the sample chamber 3 sample container carrier 8 is mounted, wherein the sample container carrier 8 is fixed by means of two retaining clips 14 fixed to the cross member 12.
  • the sample container carriers 8 are, as in particular in Fig. 3 can be clearly seen, held by the always located outside the sample chamber 3 cross member 12 of the input and export mechanism 7, even if the sample container carrier 8 is fully retracted into the sample chamber 3 and the respective access opening 4 is closed by means of the respective cover 6.
  • holding elements 15 are suitably preloaded under spring force and adapted or adapted in their position to the geometry of the sample container 2 to be used intended.
  • the total of four covers 6 are pivotally mounted on the cross member 12 of the respective input and export mechanism 7 according to arrow C about its longitudinal axis.
  • Suitable stops and guide elements on the incubator 1 or on the infeed and export mechanism 7 of the incubator 1 ensure that the cover 6 completely closes its associated access opening 4 in an upright position when the sample container carrier 8 is completely retracted by means of the infeed and export mechanism 7 and that the lid 6 immediately at the beginning of the extension of the Proben therenislves 8 from the sample chamber 3 about a predetermined by the cross member 12 pivot axis - tilted by approximately 90 ° in a horizontal position - as in the representation of the incubator 1 from Fig. 1 is shown at the top, fully extended import and export mechanism 7 there.
  • a heating and / or cooling element 16 for example in the manner of a PTC element, can be installed, which in particular by thus possible heating of the sample chamber 3 facing inside of the respective cover 6 a formation Condensate on the inside of the lid is avoidable.
  • Fig. 3 (according to the angled section line III-III Fig. 2 ) not shown in cross-section rear part of the incubator 1 serves to accommodate its electronics, not shown, and the inclusion of - dashed lines shown drive units 17 (eg electric motors) - for automatically carried out driving the run in the guides 10 rails 11 of the respective input and export mechanism. 7 which because of the given cut in Fig. 3 not shown.
  • drive units 17 eg electric motors
  • the sample chamber 3 of the incubator 1 is on five sides, namely the bottom side, ceiling side, rear side, left and right sides by firmly within the incubator 1 built sample chamber walls 18, 19, 20 21, 22 (see Fig. 3 and 4 ), which in the present example are all smooth, without openings and with curves 23, 24 between the different sides.
  • the various sample chamber walls 18, 19, 20, 21, 22 can be made at least partially from one and the same component.
  • an incubator 1 can be realized with at least one integrated input and export mechanism 7, the sample chamber 3 the assembly and arrangement of the (at least one) input and export mechanism 7 taking place outside the sample chamber 3, as described above, is particularly easy to clean.
  • an incubator 1 according to the invention can not necessarily have exactly four, but also a larger or smaller number, in particular only one access opening 4 to the sample chamber 3 that can be loaded with samples by means of a corresponding infeed and export mechanism 7.
  • Fig. 5 shows in this context an incubator 1 ', which is substantially identical to the incubator 1 from the Fig. 1 - 4th is. It differs from this only by the fact that it has a differently shaped (exchange) end plate 5 'to the front boundary of the sample chamber 3 with a smaller number, but larger access openings and corresponding thereto adapted (replacement) lids 6', which is a - and export of a total of two "deep-well microplates" are suitable. Further, in the incubator 1 ' Fig. 5 only two of the four actually import and export mechanisms 7 needed so that the unnecessary two import and export mechanisms 7 were partially removed by removing the respective rails 11 from the respective guides 10. The remaining in the outer housing 9 of the incubator 1 openings of the unneeded guides 10 are covered by appropriate cover 25 to prevent ingress of dirt into the - located outside the sample chamber 3 - guides 10.

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Claims (13)

  1. Incubateur (1) qui permet d'exposer des échantillons, contenus dans des récipients d'échantillon (2), à une température pouvant être spécifiquement définie au préalable et qui comprend
    - une chambre à échantillons (3) thermorégulable comportant au moins une ouverture d'accès (4) pouvant être obturée, et
    - au moins une mécanique d'entrée et de sortie (7) qui est pourvue d'un support de récipient d'échantillon (8) et qui permet de faire entrer le support de récipient d'échantillon (8), lequel est pourvu à son tour du récipient d'échantillon (2), dans la chambre à échantillons (3) et de le faire sortir de celle-ci, en passant une ouverture d'accès (4) de l'incubateur (1),
    caractérisé en ce que
    l'au moins une mécanique d'entrée et de sortie (7) est réalisée de manière à comporter deux guidages (10) et à ressembler à un tiroir, les deux guidages (10) étant disposés, approximativement à des hauteurs égales, sur les côtés gauche et droit chacun à l'extérieur d'une paroi (21, 22) délimitant la chambre à échantillons (3) latéralement, et le support de récipient d'échantillon (8) étant fixé sur un support transversal (12) qui relie entre eux deux rails (11) de la mécanique d'entrée et de sortie (7) lesquels sont guidés dans les deux guidages (10), et
    que l'au moins une mécanique d'entrée et de sortie (7) est montée intégralement à l'extérieur de la chambre à échantillons (3) tout en étant pourvue du support de récipient d'échantillon (8) rentré dans la chambre à échantillons (3) même lorsque l'ouverture d'accès est fermée (4).
  2. Incubateur selon la revendication 1,
    caractérisé en ce que
    l'au moins une mécanique d'entrée et de sortie (7) est disposée intégralement à l'extérieur de la chambre à échantillons (3).
  3. Incubateur selon l'une des revendications 1 ou 2,
    caractérisé en ce que
    l'au moins une ouverture d'accès (4) est fermée au moyen d'un couvercle (6), le couvercle (6) pouvant avantageusement être chauffé et/ou refroidi.
  4. Incubateur selon la revendication 3,
    caractérisé en ce que
    l'au moins un couvercle (6) destiné à fermer l'ouverture d'accès (4) est disposé sur l'au moins une mécanique d'entrée et de sortie (7) de manière à ce que le couvercle (6) ferme l'ouverture d'accès (4) lorsque le support de récipient d'échantillon (8) est complètement rentré dans la chambre à échantillons (3).
  5. Incubateur selon la revendication 1 et la revendication 4,
    caractérisé en ce que
    le couvercle (6) est fixé sur le support transversal (12) de la mécanique d'entrée et de sortie (7) ou monté pivotant sur celle-ci.
  6. Incubateur selon l'une des revendications 1 ou 5,
    caractérisé en ce que
    le support transversal (12) est entouré, au moins sur certaines parties, d'une isolation (13).
  7. Incubateur selon l'une des revendications précédentes,
    caractérisé en ce qu'
    au moins une unité d'entraînement (17) qui entraîne l'au moins une mécanique d'entrée et de sortie (7) est disposée intégralement à l'extérieur de ladite chambre à échantillons.
  8. Incubateur selon l'une des revendications précédentes,
    caractérisé en ce que
    la chambre à échantillons (3) est limitée, sur cinq de ses faces, par au moins une paroi de chambre à échantillons (18, 19, 20, 21, 22) disposée de manière fixe au sein d'un boîtier (9) de l'incubateur (1), et sur sa sixième face par un couvercle de fermeture (5, 5') présentant l'au moins une ouverture d'accès (4) pouvant être fermée.
  9. Incubateur selon la revendication 8,
    caractérisé en ce que
    l'au moins une paroi de chambre à échantillons (18, 19, 20, 21, 22) ne présente, à l'exception des percées y étant éventuellement prévues pour introduire des gaz dans la chambre à échantillons (3) et/ou pour faire passer des capteurs dans la chambre à échantillons (3), pas d'autres percées ni d'éléments rapportés qui sont montés sur la paroi de chambre à échantillons (18, 19, 20, 21, 22) ou à travers la paroi de chambre à échantillons (18, 19, 20, 21, 22).
  10. Incubateur selon les revendications 8 ou 9,
    caractérisé en ce que
    l'au moins une paroi de chambre à échantillons (18, 19, 20, 21, 22) suit, sur sa face intérieure délimitant la chambre à échantillons (3), un tracé lisse avec des parties arrondies (23, 24) entre les différentes faces de la chambre à échantillons (3).
  11. Incubateur selon l'une des revendications précédentes,
    caractérisé en ce que
    ni l'au moins une mécanique d'entrée et de sortie (7), ni le support de récipient d'échantillon (8) dont l'au moins une mécanique d'entrée et de sortie (7) est pourvue ni le récipient d'échantillon (2) ne soient en contact direct avec l'au moins une paroi de chambre à échantillons (18, 19, 20, 21, 22) quelle que soit la position de la mécanique d'entrée et de sortie (7).
  12. Incubateur selon l'une des revendications précédentes,
    caractérisé en ce que
    la chambre à échantillons (3) de l'incubateur (1) présente une pluralité d'ouvertures d'accès (4) et une pluralité de mécaniques d'entrée et de sortie (7) dont chacune est associée à une ouverture d'accès (4).
  13. Incubateur selon la revendication 12,
    caractérisé en ce que
    les ouvertures d'accès (4) de la chambre à échantillons (3) sont réalisées dans un couvercle de fermeture (5) délimitant la chambre à échantillons (3) sur l'une de ses faces, au moins deux couvercles de fermeture échangeables (5, 5') lesquels comportent un nombre différent d'ouvertures d'accès (4) et/ou des ouvertures d'accès (4) de tailles différentes étant associés à un incubateur (1).
EP13810891.5A 2013-01-07 2013-12-11 Incubateur Active EP2941480B2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102013000044.0A DE102013000044A1 (de) 2013-01-07 2013-01-07 Inkubator
PCT/EP2013/003735 WO2014106526A1 (fr) 2013-01-07 2013-12-11 Incubateur

Publications (3)

Publication Number Publication Date
EP2941480A1 EP2941480A1 (fr) 2015-11-11
EP2941480B1 EP2941480B1 (fr) 2016-08-17
EP2941480B2 true EP2941480B2 (fr) 2019-07-24

Family

ID=49816895

Family Applications (1)

Application Number Title Priority Date Filing Date
EP13810891.5A Active EP2941480B2 (fr) 2013-01-07 2013-12-11 Incubateur

Country Status (6)

Country Link
US (1) US9505002B2 (fr)
EP (1) EP2941480B2 (fr)
CN (1) CN105026543B (fr)
CA (1) CA2896952C (fr)
DE (1) DE102013000044A1 (fr)
WO (1) WO2014106526A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102014011941B3 (de) 2014-08-14 2015-08-20 Ika-Werke Gmbh & Co. Kg Einlegeboden und Inkubator
EP3255137A4 (fr) * 2015-02-05 2018-09-12 Olympus Corporation Système de culture cellulaire
ITBO20150206A1 (it) 2015-04-23 2016-10-23 Comecer Spa Incubatore modulare
EP3964818A1 (fr) * 2020-09-04 2022-03-09 Leica Mikrosysteme GmbH Préparation d'un échantillon pour congélation à haute pression
CN112195101A (zh) * 2020-09-23 2021-01-08 武汉互创联合科技有限公司 一种培养箱

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2849862A1 (fr) † 2003-01-15 2004-07-16 In Cyto Tox Dispositif et procede de culture de cellules pour essai in vitro

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US5265951A (en) * 1992-02-13 1993-11-30 Khyber Technologies Corporation Card reading terminal having protective shield for input port thereof
JP2004180675A (ja) * 2002-11-19 2004-07-02 Sanyo Electric Co Ltd インキュベータ
CN2626329Y (zh) * 2003-02-13 2004-07-21 黄成钢 双屉嵌入式保洁柜的滑轨装置
JP2004267117A (ja) 2003-03-10 2004-09-30 Olympus Corp 自動培養装置
WO2005009126A1 (fr) 2003-07-23 2005-02-03 Essen Instruments, Inc. Systèmes d'analyse de prélèvements biologiques
CN1321698C (zh) * 2004-02-06 2007-06-20 周建达 隐蔽式导轨双立门餐具消毒柜
JP4473711B2 (ja) * 2004-11-25 2010-06-02 株式会社アステック 培養装置
DE102005036763B4 (de) 2005-08-04 2007-07-26 Inheco Industrial Heating And Cooling Gmbh System aus mehreren Inkubatoren
GB2473868A (en) * 2009-09-28 2011-03-30 Invitrogen Dynal As Apparatus and method of automated processing of biological samples
US9951305B2 (en) * 2011-04-13 2018-04-24 Hitachi, Ltd. Cell culture device and transport device

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FR2849862A1 (fr) † 2003-01-15 2004-07-16 In Cyto Tox Dispositif et procede de culture de cellules pour essai in vitro

Also Published As

Publication number Publication date
US9505002B2 (en) 2016-11-29
WO2014106526A1 (fr) 2014-07-10
CN105026543B (zh) 2018-04-17
CN105026543A (zh) 2015-11-04
CA2896952A1 (fr) 2014-07-10
EP2941480A1 (fr) 2015-11-11
EP2941480B1 (fr) 2016-08-17
CA2896952C (fr) 2022-12-06
US20150306600A1 (en) 2015-10-29
DE102013000044A1 (de) 2014-07-10

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