EP3443084A4 - Procédés de création de chromosomes synthétiques dotés de systèmes régulateurs de gènes et leurs utilisations - Google Patents

Procédés de création de chromosomes synthétiques dotés de systèmes régulateurs de gènes et leurs utilisations Download PDF

Info

Publication number
EP3443084A4
EP3443084A4 EP17783084.1A EP17783084A EP3443084A4 EP 3443084 A4 EP3443084 A4 EP 3443084A4 EP 17783084 A EP17783084 A EP 17783084A EP 3443084 A4 EP3443084 A4 EP 3443084A4
Authority
EP
European Patent Office
Prior art keywords
methods
gene regulatory
regulatory systems
creating synthetic
synthetic chromosomes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP17783084.1A
Other languages
German (de)
English (en)
Other versions
EP3443084A2 (fr
Inventor
Edward Perkins
Amy Greene
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carrygenes Bioengineering LLC
Original Assignee
Synploid Biotek LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synploid Biotek LLC filed Critical Synploid Biotek LLC
Priority to EP21173114.6A priority Critical patent/EP3910058B1/fr
Priority to EP24217879.6A priority patent/EP4495248A3/fr
Publication of EP3443084A2 publication Critical patent/EP3443084A2/fr
Publication of EP3443084A4 publication Critical patent/EP3443084A4/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3007—Carcino-embryonic Antigens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Oncology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP17783084.1A 2016-04-12 2017-04-12 Procédés de création de chromosomes synthétiques dotés de systèmes régulateurs de gènes et leurs utilisations Pending EP3443084A4 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP21173114.6A EP3910058B1 (fr) 2016-04-12 2017-04-12 Procédés de création de chromosomes synthétiques dotés de systèmes régulateurs de gènes et leurs utilisations
EP24217879.6A EP4495248A3 (fr) 2016-04-12 2017-04-12 Procédés de création de chromosomes synthétiques ayant des systèmes de régulation génique et leurs utilisations

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662321720P 2016-04-12 2016-04-12
PCT/US2017/027270 WO2017180786A2 (fr) 2016-04-12 2017-04-12 Procédés de création de chromosomes synthétiques dotés de systèmes régulateurs de gènes et leurs utilisations

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP21173114.6A Division EP3910058B1 (fr) 2016-04-12 2017-04-12 Procédés de création de chromosomes synthétiques dotés de systèmes régulateurs de gènes et leurs utilisations
EP24217879.6A Division EP4495248A3 (fr) 2016-04-12 2017-04-12 Procédés de création de chromosomes synthétiques ayant des systèmes de régulation génique et leurs utilisations

Publications (2)

Publication Number Publication Date
EP3443084A2 EP3443084A2 (fr) 2019-02-20
EP3443084A4 true EP3443084A4 (fr) 2019-11-13

Family

ID=60042729

Family Applications (3)

Application Number Title Priority Date Filing Date
EP17783084.1A Pending EP3443084A4 (fr) 2016-04-12 2017-04-12 Procédés de création de chromosomes synthétiques dotés de systèmes régulateurs de gènes et leurs utilisations
EP21173114.6A Active EP3910058B1 (fr) 2016-04-12 2017-04-12 Procédés de création de chromosomes synthétiques dotés de systèmes régulateurs de gènes et leurs utilisations
EP24217879.6A Pending EP4495248A3 (fr) 2016-04-12 2017-04-12 Procédés de création de chromosomes synthétiques ayant des systèmes de régulation génique et leurs utilisations

Family Applications After (2)

Application Number Title Priority Date Filing Date
EP21173114.6A Active EP3910058B1 (fr) 2016-04-12 2017-04-12 Procédés de création de chromosomes synthétiques dotés de systèmes régulateurs de gènes et leurs utilisations
EP24217879.6A Pending EP4495248A3 (fr) 2016-04-12 2017-04-12 Procédés de création de chromosomes synthétiques ayant des systèmes de régulation génique et leurs utilisations

Country Status (8)

Country Link
US (1) US12559567B2 (fr)
EP (3) EP3443084A4 (fr)
JP (3) JP2019513396A (fr)
CN (1) CN109415719A (fr)
AU (1) AU2017250265B2 (fr)
CA (1) CA3020984A1 (fr)
RU (1) RU2018139465A (fr)
WO (1) WO2017180786A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
WO2015105955A1 (fr) 2014-01-08 2015-07-16 Flodesign Sonics, Inc. Dispositif d'acoustophorèse avec double chambre acoustophorétique
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US12559567B2 (en) * 2016-04-12 2026-02-24 Carrygenes Bioengineering, Llc Methods for creating synthetic chromosomes having gene regulatory systems and uses thereof
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
WO2019118921A1 (fr) 2017-12-14 2019-06-20 Flodesign Sonics, Inc. Circuit d'excitation et circuit de commande de transducteur acoustique
WO2020209458A1 (fr) * 2019-04-12 2020-10-15 주식회사 휴맵 Chromosome recombiné artificiel et son utilisation
CN114787373A (zh) * 2019-07-25 2022-07-22 免疫苏醒公司 测量通过效应子进行的细胞介导的杀伤的方法
EP4395838A1 (fr) 2021-08-30 2024-07-10 CarryGenes Bioengineering, LLC tCD34 ET AUTRES MARQUEURS POUR L'IDENTIFICATION ET LE TRI DE CELLULES ET POUR UNE UTILISATION EN TANT QU'ASSISTANT DE SUIV IN VIVO

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4966843A (en) 1982-11-01 1990-10-30 Cetus Corporation Expression of interferon genes in Chinese hamster ovary cells
US4833080A (en) 1985-12-12 1989-05-23 President And Fellows Of Harvard College Regulation of eucaryotic gene expression
US5681713A (en) 1987-05-08 1997-10-28 Smithkline Beecham Corporation Expression of heterologous proteins in Drosophila cells
US5011776A (en) 1988-02-11 1991-04-30 The Royal Institution For The Advancement Of Learning (Mcgill University) Shuttle vector, utilizing the E. coli asparagine synthetase gene, and capable of dominant transfection and amplification in animal cells
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5747308A (en) 1989-10-25 1998-05-05 Celltech Therapeutics Limited Recombinant DNA method
US5658772A (en) 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
CA2042093C (fr) 1990-05-09 2002-12-24 Gyula Hadlaczky Lignee de cellules renfermant un exces de centromeres mammaliens
US5288625A (en) 1991-09-13 1994-02-22 Biologic Research Center Of The Hungarian Academy Of Sciences Mammalian artificial chromosomes
JP3314241B2 (ja) 1992-04-06 2002-08-12 ヤマハ発動機株式会社 自動二輪車用エンジンの排気浄化装置
US5527695A (en) 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
US5869294A (en) 1995-06-07 1999-02-09 Case Western Reserve University Method for stably cloning large repeating DNA sequences
US5695967A (en) 1995-06-07 1997-12-09 Case Western Reserve University Method for stably cloning large repeating units of DNA
US5721118A (en) 1995-10-31 1998-02-24 The Regents Of The University Of California, San Diego Mammalian artificial chromosomes and methods of using same
US20030033617A1 (en) 1996-04-10 2003-02-13 Gyula Hadlaczky Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US6025155A (en) 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US6077697A (en) 1996-04-10 2000-06-20 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
WO1998008964A1 (fr) 1996-08-26 1998-03-05 Tsuneko Okazaki Chromosomes artificiels de mammifere
US7585622B1 (en) 1996-10-01 2009-09-08 Geron Corporation Increasing the proliferative capacity of cells using telomerase reverse transcriptase
JP3151657B2 (ja) 1997-03-26 2001-04-03 農林水産省農業生物資源研究所長 遺伝子導入法
US6365373B2 (en) 1997-04-25 2002-04-02 Genentech, Inc. Nucleic acids encoding NGF variants
DE69908308T2 (de) 1998-02-20 2004-05-06 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Molekular-rechenelemente : gates und flip-flops
GB9821193D0 (en) 1998-09-30 1998-11-25 Medical Res Council Mammalian artificial chromosomes and uses thereof
ATE452972T1 (de) * 2001-05-01 2010-01-15 Ca Nat Research Council Induzierbares expressionssystem in eukaryotischen zellen
AU2002310275B2 (en) 2001-05-30 2006-08-31 Glaxo Group Limited Chromosome-based platforms
WO2002096923A1 (fr) 2001-05-30 2002-12-05 Chromos Molecular Systems, Inc. Chromosomes artificiels de plantes, utilisation de ces derniers et procedes de preparation de chromosomes artificiels de plantes
AU2003261863C1 (en) 2002-09-03 2008-06-12 Japan Science And Technology Agency Artificial mammalian chromosome
US20060185025A1 (en) 2002-10-04 2006-08-17 Kirin Beer Kabushiki Kaisha Human artificial chromosome (hac) vector
US7267984B2 (en) 2002-10-31 2007-09-11 Rice University Recombination assembly of large DNA fragments
WO2009063722A1 (fr) 2007-11-14 2009-05-22 National University Corporation Tottori University Vecteur chromosomique artificiel de mammifère portant le gène (groupe de gènes) de cytochrome p450 humain, et mammifère non humain portant le vecteur
EP2522725B1 (fr) 2010-01-06 2016-10-05 National University Corporation Tottori University Vecteur de chromosome artificiel de souris
US9458509B2 (en) * 2010-07-22 2016-10-04 President And Fellows Of Harvard College Multiple input biologic classifier circuits for cells
HU230368B1 (hu) 2012-11-16 2016-03-29 Magyar Tudományos Akadémia Szegedi Biológiai Kutatóközpont Új módszer az emlős mesterséges kromoszóma több génnel való feltöltésére
WO2015006294A2 (fr) * 2013-07-10 2015-01-15 President And Fellows Of Harvard College Protéines cas9 orthogonales pour la régulation et l'édition de gènes avec arn guide
WO2015066722A1 (fr) * 2013-11-04 2015-05-07 Bgn Tech Llc Procédés de préparation de vanilline par fermentation microbienne d'acide férulique à partir d'eugénol au moyen d'une déshydrogénase végétale
CA2975526C (fr) 2015-02-09 2023-11-07 Edward Perkins Compositions et methodes pour la surveillance de la construction en temps reel et du genie genetique biomedical de chromosomes synthetiques de mammifere
US12559567B2 (en) * 2016-04-12 2026-02-24 Carrygenes Bioengineering, Llc Methods for creating synthetic chromosomes having gene regulatory systems and uses thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MARTELLA ANDREA ET AL: "Mammalian Synthetic Biology: Time for Big MACs", ACS SYNTHETIC BIOLOGY, vol. 5, no. 10, Sp. Iss. SI, October 2016 (2016-10-01), pages 1040 - 1049, XP002794563, ISSN: 2161-5063 *
SHIGEYUKI YAMAGUCHI ET AL: "A Method for Producing Transgenic Cells Using a Multi-Integrase System on a Human Artificial Chromosome Vector", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 6, no. 2, 24 February 2011 (2011-02-24), pages e17267 - 1, XP002741727, ISSN: 1932-6203, DOI: 10.1371/JOURNAL.PONE.0017267 *
TERUHIKO SUZUKI ET AL: "A Novel System for Simultaneous or Sequential Integration of Multiple Gene-Loading Vectors into a Defined Site of a Human Artificial Chromosome", PLOS ONE, vol. 9, no. 10, 10 October 2014 (2014-10-10), pages 1 - 9, XP055386725, DOI: 10.1371/journal.pone.0110404 *

Also Published As

Publication number Publication date
EP3910058B1 (fr) 2025-01-08
AU2017250265B2 (en) 2023-08-17
JP2025071134A (ja) 2025-05-02
CN109415719A (zh) 2019-03-01
AU2017250265A1 (en) 2018-11-22
EP3443084A2 (fr) 2019-02-20
JP2019513396A (ja) 2019-05-30
EP3910058A1 (fr) 2021-11-17
EP4495248A2 (fr) 2025-01-22
WO2017180786A3 (fr) 2017-11-23
US20190345259A1 (en) 2019-11-14
WO2017180786A2 (fr) 2017-10-19
JP2022097481A (ja) 2022-06-30
RU2018139465A (ru) 2020-05-13
US12559567B2 (en) 2026-02-24
EP3910058C0 (fr) 2025-01-08
CA3020984A1 (fr) 2017-10-19
EP4495248A3 (fr) 2025-04-02

Similar Documents

Publication Publication Date Title
EP3443084A4 (fr) Procédés de création de chromosomes synthétiques dotés de systèmes régulateurs de gènes et leurs utilisations
EP3358940A4 (fr) Systèmes et procédés de culture
EP3700980A4 (fr) Systèmes particulaires et procédés
EP3420456A4 (fr) Systèmes et procédés anti-répétitions
EP3426338A4 (fr) Systèmes et procédés de neurostimulation de contractilité cardiaque
EP3676335A4 (fr) Systèmes particulaires et procédés
EP3676901A4 (fr) Systèmes et procédés de particules
EP3522814A4 (fr) Systèmes et procédés de télé-chirurgie assistée par ordinateur
IL261126A (en) Methods and systems for evaluating tumor mutational burden
EP3512451A4 (fr) Systèmes et procédés de télé-chirurgie assistée par ordinateur
EP3468498A4 (fr) Systèmes et procédés de télé-chirurgie assistée par ordinateur
EP3523783A4 (fr) Systèmes et procédés de modélisation de surface
EP3669166A4 (fr) Systèmes et procédés de séparation de particules
EP3513265A4 (fr) Systèmes et procédés de détermination de quasi-collision
EP3384468A4 (fr) Systèmes et procédés de relocalisation
EP3586297A4 (fr) Systèmes et procédés de covoiturage
MA41507A (fr) Systèmes et procédés de production de polymères
EP3353298A4 (fr) Édition de gènes allèle sélective et utilisations associées
EP3303188A4 (fr) Système de stockage et de récupération
EP3383904A4 (fr) Anticorps ctla-4 et leurs utilisations
MA53145A (fr) Agents de liaison cd123 et leurs utilisations
EP3451919A4 (fr) Systèmes et procédés de neuro-rétroaction
EP3334706A4 (fr) Pillararènes et utilisations de ceux-ci
EP3538261A4 (fr) Catalyseurs en pérovskite et leurs utilisations
EP3353679A4 (fr) Systèmes et procédés de reconnaissance de points intéressants

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20181101

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 15/87 20060101ALI20190927BHEP

Ipc: C12N 15/63 20060101ALI20190927BHEP

Ipc: C12N 15/85 20060101ALI20190927BHEP

Ipc: C12N 15/09 20060101AFI20190927BHEP

Ipc: C12N 15/90 20060101ALI20190927BHEP

A4 Supplementary search report drawn up and despatched

Effective date: 20191014

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

17Q First examination report despatched

Effective date: 20200716

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: CARRYGENES BIOENGINEERING, LLC

RAP3 Party data changed (applicant data changed or rights of an application transferred)

Owner name: CARRYGENES BIOENGINEERING, LLC

RIN1 Information on inventor provided before grant (corrected)

Inventor name: GREENE, AMY

Inventor name: PERKINS, EDWARD

RIN1 Information on inventor provided before grant (corrected)

Inventor name: GREENE, AMY

Inventor name: PERKINS, EDWARD