EP4720331A1 - Falsch-positive reduktion durch reparatur der translesion-polymerase - Google Patents

Falsch-positive reduktion durch reparatur der translesion-polymerase

Info

Publication number
EP4720331A1
EP4720331A1 EP24734742.0A EP24734742A EP4720331A1 EP 4720331 A1 EP4720331 A1 EP 4720331A1 EP 24734742 A EP24734742 A EP 24734742A EP 4720331 A1 EP4720331 A1 EP 4720331A1
Authority
EP
European Patent Office
Prior art keywords
altered
cytosine
subfamily
stranded dna
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24734742.0A
Other languages
English (en)
French (fr)
Inventor
Kayla BUSBY
Gaetano SPECIALE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of EP4720331A1 publication Critical patent/EP4720331A1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP24734742.0A 2023-05-31 2024-05-29 Falsch-positive reduktion durch reparatur der translesion-polymerase Pending EP4720331A1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363469860P 2023-05-31 2023-05-31
PCT/US2024/031370 WO2024249466A1 (en) 2023-05-31 2024-05-29 False positive reduction by translesion polymerase repair

Publications (1)

Publication Number Publication Date
EP4720331A1 true EP4720331A1 (de) 2026-04-08

Family

ID=91585818

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24734742.0A Pending EP4720331A1 (de) 2023-05-31 2024-05-29 Falsch-positive reduktion durch reparatur der translesion-polymerase

Country Status (2)

Country Link
EP (1) EP4720331A1 (de)
WO (1) WO2024249466A1 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2026006774A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Altered cytidine deaminases and methods of use

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
WO2004018497A2 (en) 2002-08-23 2004-03-04 Solexa Limited Modified nucleotides for polynucleotide sequencing
EP1701785A1 (de) 2004-01-07 2006-09-20 Solexa Ltd. Modifizierte molekülarrays
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
GB2457851B (en) 2006-12-14 2011-01-05 Ion Torrent Systems Inc Methods and apparatus for measuring analytes using large scale fet arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
CN107406890B (zh) 2015-02-10 2023-07-18 亿明达股份有限公司 用于分析细胞组分的方法和组合物
EP3904514A1 (de) 2016-07-22 2021-11-03 Oregon Health & Science University Einzellige gesamtgenombibliotheken und kombinatorische indexierungsverfahren zur herstellung davon
WO2018057779A1 (en) * 2016-09-23 2018-03-29 Jianbiao Zheng Compositions of synthetic transposons and methods of use thereof
EP3913053A1 (de) 2017-04-23 2021-11-24 Illumina Cambridge Limited Zusammensetzungen und verfahren zur verbesserung der probenidentifikation in indizierten nukleinsäurebibliotheken
DK3810774T3 (da) 2018-06-04 2023-12-11 Illumina Inc Enkeltcelletransskriptom-biblioteker med højt throughput og fremgangsmåder til fremstilling og anvendelse
WO2022159715A1 (en) * 2021-01-22 2022-07-28 The Broad Institute, Inc. Tracking apobec mutational signatures in tumor cells
CN115261363B (zh) * 2021-04-29 2024-01-30 中国科学院分子植物科学卓越创新中心 Apobec3a的rna脱氨酶活性测定方法及rna高活性的apobec3a变体
CA3246669A1 (en) * 2023-01-06 2024-07-11 Illumina, Inc. URACIL REDUCTION BY POLYMERASE

Also Published As

Publication number Publication date
WO2024249466A1 (en) 2024-12-05

Similar Documents

Publication Publication Date Title
US20240182881A1 (en) Altered cytidine deaminases and methods of use
US10704091B2 (en) Genotyping by next-generation sequencing
EP2623613B1 (de) Erhöhung der Verlässlichkeit von Allel-Aufrufen mit Molekülzählung
US20250327067A1 (en) Reducing uracils by polymerase
KR102313470B1 (ko) Dna의 에러-프리 염기서열 분석
WO2024118903A1 (en) Chemoenzymatic correction of false positive uracil transformations
US20250388894A1 (en) Methods of using cpg binding proteins in mapping modified cytosine nucleotides
US10100292B2 (en) Mutant endonuclease V enzymes and applications thereof
EP4594482A1 (de) Cytidindeaminasen und verfahren zur verwendung bei der kartierung modifizierter cytosinnukleotide
JP2022511207A (ja) ブリッジ増幅によるクラスタ生成のための方法および組成物
WO2025072800A2 (en) Altered cytidine deaminases and methods of use
EP4594481A1 (de) Helicase-cytidindeaminase-komplexe und verfahren zur verwendung
EP4720331A1 (de) Falsch-positive reduktion durch reparatur der translesion-polymerase
WO2025081064A2 (en) Thermophilic deaminase and methods for identifying modified cytosine
Reiter et al. RT-PCR optimization strategies
WO2026006774A1 (en) Altered cytidine deaminases and methods of use
JP2008178338A (ja) 断片化核酸が混入する核酸試料中の標的核酸を増幅する核酸増幅方法、及びそのキット
Beaulne Nature and structure of DNA through the process of degradation
HK1204337B (en) Genotyping by next-generation sequencing

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: UNKNOWN

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20251209

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR