EP4720331A1 - Réduction de faux positifs par réparation de polymérase par translésion - Google Patents

Réduction de faux positifs par réparation de polymérase par translésion

Info

Publication number
EP4720331A1
EP4720331A1 EP24734742.0A EP24734742A EP4720331A1 EP 4720331 A1 EP4720331 A1 EP 4720331A1 EP 24734742 A EP24734742 A EP 24734742A EP 4720331 A1 EP4720331 A1 EP 4720331A1
Authority
EP
European Patent Office
Prior art keywords
altered
cytosine
subfamily
stranded dna
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24734742.0A
Other languages
German (de)
English (en)
Inventor
Kayla BUSBY
Gaetano SPECIALE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of EP4720331A1 publication Critical patent/EP4720331A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés, des compositions et des kits pour éliminer les faux uraciles positifs en raison de la désamination de cytosines non méthylées dans des dosages à l'aide de cytosine désaminases modifiées pour désaminer des cytosines méthylées. Les procédés, les compositions et les kits utilisent un procédé enzymatique double. Après le traitement de la cytidine désaminase, l'ADN est d'abord incubé avec de l'uracile ADN glycosylase (UDG), qui génère des sites abasiques où des conversions de dC en dU se sont produites. L'ADN est ensuite incubé avec une polymérase à haute fidélité complétée par une désoxycytidyl transférase, telle que Rev1, pour réparer la lésion avec l'installation d'une cytidine.
EP24734742.0A 2023-05-31 2024-05-29 Réduction de faux positifs par réparation de polymérase par translésion Pending EP4720331A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363469860P 2023-05-31 2023-05-31
PCT/US2024/031370 WO2024249466A1 (fr) 2023-05-31 2024-05-29 Réduction de faux positifs par réparation de polymérase par translésion

Publications (1)

Publication Number Publication Date
EP4720331A1 true EP4720331A1 (fr) 2026-04-08

Family

ID=91585818

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24734742.0A Pending EP4720331A1 (fr) 2023-05-31 2024-05-29 Réduction de faux positifs par réparation de polymérase par translésion

Country Status (2)

Country Link
EP (1) EP4720331A1 (fr)
WO (1) WO2024249466A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2026006774A1 (fr) 2024-06-28 2026-01-02 Illumina, Inc. Cytidine désaminases modifiées et procédés d'utilisation

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
WO2004018497A2 (fr) 2002-08-23 2004-03-04 Solexa Limited Nucleotides modifies
EP1701785A1 (fr) 2004-01-07 2006-09-20 Solexa Ltd. Reseaux moleculaires modifies
WO2006064199A1 (fr) 2004-12-13 2006-06-22 Solexa Limited Procede ameliore de detection de nucleotides
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
GB2457851B (en) 2006-12-14 2011-01-05 Ion Torrent Systems Inc Methods and apparatus for measuring analytes using large scale fet arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
CN107406890B (zh) 2015-02-10 2023-07-18 亿明达股份有限公司 用于分析细胞组分的方法和组合物
EP3904514A1 (fr) 2016-07-22 2021-11-03 Oregon Health & Science University Bibliothèques de génome entier de cellules uniques et procédés d'indexage combinatoire pour leur fabrication
WO2018057779A1 (fr) * 2016-09-23 2018-03-29 Jianbiao Zheng Compositions de transposons synthétiques et leurs procédés d'utilisation
EP3913053A1 (fr) 2017-04-23 2021-11-24 Illumina Cambridge Limited Compositions et procédés permettant d'améliorer l'identification d'échantillons dans des bibliothèques d'acides nucléiques indexés
DK3810774T3 (da) 2018-06-04 2023-12-11 Illumina Inc Enkeltcelletransskriptom-biblioteker med højt throughput og fremgangsmåder til fremstilling og anvendelse
WO2022159715A1 (fr) * 2021-01-22 2022-07-28 The Broad Institute, Inc. Suivi de signatures mutationnelles d'apobec dans des cellules tumorales
CN115261363B (zh) * 2021-04-29 2024-01-30 中国科学院分子植物科学卓越创新中心 Apobec3a的rna脱氨酶活性测定方法及rna高活性的apobec3a变体
CA3246669A1 (fr) * 2023-01-06 2024-07-11 Illumina, Inc. Réduction des uraciles par polymérase

Also Published As

Publication number Publication date
WO2024249466A1 (fr) 2024-12-05

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