EP4720341A1 - Identification et cartographie de sites de méthylation - Google Patents

Identification et cartographie de sites de méthylation

Info

Publication number
EP4720341A1
EP4720341A1 EP24737230.3A EP24737230A EP4720341A1 EP 4720341 A1 EP4720341 A1 EP 4720341A1 EP 24737230 A EP24737230 A EP 24737230A EP 4720341 A1 EP4720341 A1 EP 4720341A1
Authority
EP
European Patent Office
Prior art keywords
dna
adapter
double
stranded
strand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24737230.3A
Other languages
German (de)
English (en)
Inventor
Daniel Wolf
Xi-jun CHEN
Pietro Gatti Lafranconi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of EP4720341A1 publication Critical patent/EP4720341A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente divulgation concerne des procédés d'identification et de cartographie de sites de méthylation. Dans certains modes de réalisation, ces procédés incorporent une désamination de cytosine et une réparation d'excision de bases. Dans certains modes de réalisation, ces procédés incorporent l'utilisation d'adaptateurs en épingle à cheveux. L'invention concerne également des procédés d'analyse de séquences.
EP24737230.3A 2023-06-05 2024-06-03 Identification et cartographie de sites de méthylation Pending EP4720341A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363506181P 2023-06-05 2023-06-05
PCT/US2024/032260 WO2024254003A1 (fr) 2023-06-05 2024-06-03 Identification et cartographie de sites de méthylation

Publications (1)

Publication Number Publication Date
EP4720341A1 true EP4720341A1 (fr) 2026-04-08

Family

ID=91738690

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24737230.3A Pending EP4720341A1 (fr) 2023-06-05 2024-06-03 Identification et cartographie de sites de méthylation

Country Status (2)

Country Link
EP (1) EP4720341A1 (fr)
WO (1) WO2024254003A1 (fr)

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006678A1 (fr) 1989-10-26 1991-05-16 Sri International Sequençage d'adn
EP2319854B1 (fr) 1997-01-08 2016-11-30 Sigma-Aldrich Co. LLC Bio-conjugaison de macromolécules
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
US7427678B2 (en) 1998-01-08 2008-09-23 Sigma-Aldrich Co. Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
ATE377093T1 (de) 2000-07-07 2007-11-15 Visigen Biotechnologies Inc Sequenzbestimmung in echtzeit
AU2002227156A1 (en) 2000-12-01 2002-06-11 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
JP4638225B2 (ja) 2002-05-30 2011-02-23 ザ スクリプス リサーチ インスティテュート 銅を触媒とするアジドとアセチレンのライゲーション
WO2004018497A2 (fr) 2002-08-23 2004-03-04 Solexa Limited Nucleotides modifies
US7259258B2 (en) 2003-12-17 2007-08-21 Illumina, Inc. Methods of attaching biological compounds to solid supports using triazine
EP1701785A1 (fr) 2004-01-07 2006-09-20 Solexa Ltd. Reseaux moleculaires modifies
CA2579150C (fr) 2004-09-17 2014-11-25 Pacific Biosciences Of California, Inc. Appareil et procede d'analyse de molecules
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
CA2648149A1 (fr) 2006-03-31 2007-11-01 Solexa, Inc. Systemes et procedes pour analyse de sequencage par synthese
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
EP2718465B1 (fr) 2011-06-09 2022-04-13 Illumina, Inc. Procede de fabrication d'un reseau d'analytes
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
CA3060708A1 (fr) 2014-04-29 2015-11-05 Illumina, Inc Analyse de l'expression de genes de cellules isolees multiplexees par commutation de matrice et fragmentation et etiquetage (tagmentation)
KR102866805B1 (ko) 2017-02-21 2025-09-30 일루미나, 인코포레이티드 링커를 갖는 고정된 트랜스포좀을 사용한 태그먼트화
US20220325316A1 (en) * 2019-08-09 2022-10-13 Battelle Memorial Institute Compositions and methods for detecting methylated dna
CN116043337A (zh) * 2019-08-27 2023-05-02 江苏鹍远生物科技股份有限公司 Dna甲基化标志物筛查试剂盒及方法
CN115125624A (zh) * 2021-03-25 2022-09-30 南方医科大学 一组条码接头以及中通量多重单细胞代表性dna甲基化建库和测序方法
AU2022333075A1 (en) * 2021-08-26 2024-01-04 Illumina, Inc. Methods and compositions for detecting genomic methylation

Also Published As

Publication number Publication date
WO2024254003A1 (fr) 2024-12-12
WO2024254003A9 (fr) 2025-01-09

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