EP4728091A1 - Gleichzeitige sequenzierung mit räumlich getrennten ringen - Google Patents

Gleichzeitige sequenzierung mit räumlich getrennten ringen

Info

Publication number
EP4728091A1
EP4728091A1 EP24734826.1A EP24734826A EP4728091A1 EP 4728091 A1 EP4728091 A1 EP 4728091A1 EP 24734826 A EP24734826 A EP 24734826A EP 4728091 A1 EP4728091 A1 EP 4728091A1
Authority
EP
European Patent Office
Prior art keywords
immobilised
proportion
primers
solid support
immobilised primers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24734826.1A
Other languages
English (en)
French (fr)
Inventor
Aathavan KARUNAKARAN
Jonathan Boutell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of EP4728091A1 publication Critical patent/EP4728091A1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP24734826.1A 2023-06-14 2024-06-13 Gleichzeitige sequenzierung mit räumlich getrennten ringen Pending EP4728091A1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363508070P 2023-06-14 2023-06-14
PCT/EP2024/066446 WO2024256580A1 (en) 2023-06-14 2024-06-13 Concurrent sequencing with spatially separated rings

Publications (1)

Publication Number Publication Date
EP4728091A1 true EP4728091A1 (de) 2026-04-22

Family

ID=91616878

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24734826.1A Pending EP4728091A1 (de) 2023-06-14 2024-06-13 Gleichzeitige sequenzierung mit räumlich getrennten ringen

Country Status (2)

Country Link
EP (1) EP4728091A1 (de)
WO (1) WO2024256580A1 (de)

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
EP1498494A3 (de) 1997-04-01 2007-06-20 Solexa Ltd. Methode zur Sequenzierung von Nukleinsäuren
EP1591541B1 (de) 1997-04-01 2012-02-15 Illumina Cambridge Limited Verfahren zur Vervielfältigung von Nukleinsäuren
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
EP1301591A4 (de) 2000-07-13 2004-05-26 Invitrogen Corp Verfahren und zusammensetzungen zur schnellen protein- und peptidextraktion und -isolierung mittels lysismatrize
EP1483404A2 (de) 2002-03-05 2004-12-08 Solexa Ltd. Methoden zur detektion genom-überspannender sequenzvariationen, welche mit einem phenotyp assoziiert sind
EP1682680B2 (de) 2003-10-31 2018-03-21 AB Advanced Genetic Analysis Corporation Verfahren zur herstellung eines gepaarten tag aus einer nukleinsäuresequenz sowie verfahren zur verwendung davon
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
EP2272983A1 (de) 2005-02-01 2011-01-12 AB Advanced Genetic Analysis Corporation Reagentien, Verfahren und Bibliotheken zur Sequenzierung mit Kügelchen
EP1877576B1 (de) 2005-04-12 2013-01-23 454 Life Sciences Corporation Verfahren zur bestimmung von sequenzvarianten mittels ultra-deep-sequenzierung
CA2615323A1 (en) 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
US8428882B2 (en) 2005-06-14 2013-04-23 Agency For Science, Technology And Research Method of processing and/or genome mapping of diTag sequences
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
EP1987159B2 (de) 2006-02-08 2020-08-12 Illumina Cambridge Limited Verfahren zur sequenzierung einer polynukleotidmatrize
EP2021503A1 (de) 2006-03-17 2009-02-11 Solexa Ltd. Isothermische methoden zur entstehung von arrays von einzelnen molekülen
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
ES2743029T3 (es) 2008-10-24 2020-02-18 Epicentre Tech Corporation Composiciones de extremo del transposón y métodos para modificar ácidos nucleicos
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
EP3623481B1 (de) 2011-09-23 2021-08-25 Illumina, Inc. Zusammensetzungen zur nukleinsäuresequenzierung
US20130143774A1 (en) 2011-12-05 2013-06-06 The Regents Of The University Of California Methods and compositions for generating polynucleic acid fragments
EP2820155B1 (de) * 2012-02-28 2017-07-26 Population Genetics Technologies Ltd. Verfahren zur befestigung einer zählersequenz an einer nucleinsäureprobe
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
WO2016075204A1 (en) * 2014-11-11 2016-05-19 Illumina, Inc. Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
EP4603581A3 (de) 2015-05-28 2025-11-12 Illumina Cambridge Limited Oberflächenbasierte tagmentation
WO2019136376A1 (en) 2018-01-08 2019-07-11 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
US12084474B2 (en) 2018-05-15 2024-09-10 Illumina, Inc. Compositions and methods for chemical cleavage and deprotection of surface-bound oligonucleotides
AU2021366658A1 (en) 2020-10-21 2023-06-22 Illumina Cambridge Limited Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput

Also Published As

Publication number Publication date
WO2024256580A1 (en) 2024-12-19

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