EP4728092A1 - Bestimmung von modifizierten cytosinen - Google Patents

Bestimmung von modifizierten cytosinen

Info

Publication number
EP4728092A1
EP4728092A1 EP24735888.0A EP24735888A EP4728092A1 EP 4728092 A1 EP4728092 A1 EP 4728092A1 EP 24735888 A EP24735888 A EP 24735888A EP 4728092 A1 EP4728092 A1 EP 4728092A1
Authority
EP
European Patent Office
Prior art keywords
strand
polynucleotide
hairpin
sequence
library
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24735888.0A
Other languages
English (en)
French (fr)
Inventor
Colin Brown
Sarah SHULTZABERGER
Aathavan KARUNAKARAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of EP4728092A1 publication Critical patent/EP4728092A1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP24735888.0A 2023-06-14 2024-06-13 Bestimmung von modifizierten cytosinen Pending EP4728092A1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363508166P 2023-06-14 2023-06-14
PCT/EP2024/066447 WO2024256581A1 (en) 2023-06-14 2024-06-13 Determination of modified cytosines

Publications (1)

Publication Number Publication Date
EP4728092A1 true EP4728092A1 (de) 2026-04-22

Family

ID=91664534

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24735888.0A Pending EP4728092A1 (de) 2023-06-14 2024-06-13 Bestimmung von modifizierten cytosinen

Country Status (2)

Country Link
EP (1) EP4728092A1 (de)
WO (1) WO2024256581A1 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025254779A1 (en) * 2024-06-06 2025-12-11 Epicrispr Biotechnologies, Inc. Engineered epigenetic effectors

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1591541B1 (de) 1997-04-01 2012-02-15 Illumina Cambridge Limited Verfahren zur Vervielfältigung von Nukleinsäuren
EP1498494A3 (de) 1997-04-01 2007-06-20 Solexa Ltd. Methode zur Sequenzierung von Nukleinsäuren
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
EP1301591A4 (de) 2000-07-13 2004-05-26 Invitrogen Corp Verfahren und zusammensetzungen zur schnellen protein- und peptidextraktion und -isolierung mittels lysismatrize
EP1483404A2 (de) 2002-03-05 2004-12-08 Solexa Ltd. Methoden zur detektion genom-überspannender sequenzvariationen, welche mit einem phenotyp assoziiert sind
EP1682680B2 (de) 2003-10-31 2018-03-21 AB Advanced Genetic Analysis Corporation Verfahren zur herstellung eines gepaarten tag aus einer nukleinsäuresequenz sowie verfahren zur verwendung davon
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
EP1877576B1 (de) 2005-04-12 2013-01-23 454 Life Sciences Corporation Verfahren zur bestimmung von sequenzvarianten mittels ultra-deep-sequenzierung
CA2615323A1 (en) 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
US8428882B2 (en) 2005-06-14 2013-04-23 Agency For Science, Technology And Research Method of processing and/or genome mapping of diTag sequences
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
EP1987159B2 (de) 2006-02-08 2020-08-12 Illumina Cambridge Limited Verfahren zur sequenzierung einer polynukleotidmatrize
EP2021503A1 (de) 2006-03-17 2009-02-11 Solexa Ltd. Isothermische methoden zur entstehung von arrays von einzelnen molekülen
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
EP3623481B1 (de) 2011-09-23 2021-08-25 Illumina, Inc. Zusammensetzungen zur nukleinsäuresequenzierung
US9121061B2 (en) 2012-03-15 2015-09-01 New England Biolabs, Inc. Methods and compositions for discrimination between cytosine and modifications thereof and for methylome analysis
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US20150011396A1 (en) * 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
EP3845668A1 (de) 2015-10-30 2021-07-07 New England Biolabs, Inc. Zusammensetzungen und verfahren zur bestimmung von modifizierten cytosinen durch sequenzierung
WO2019136376A1 (en) 2018-01-08 2019-07-11 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
EP4007818A4 (de) * 2019-08-01 2023-09-20 Twinstrand Biosciences, Inc. Verfahren und reagenzien zum sequenzieren von nukleinsäuren und zugehörige anwendungen
US11359238B2 (en) * 2020-03-06 2022-06-14 Singular Genomics Systems, Inc. Linked paired strand sequencing
AU2021366658A1 (en) 2020-10-21 2023-06-22 Illumina Cambridge Limited Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput
EP4396339A4 (de) * 2021-09-03 2025-08-06 Singular Genomics Systems Inc Verfahren zur differenzierung modifizierter nukleobasen

Also Published As

Publication number Publication date
WO2024256581A1 (en) 2024-12-19

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