EP4731784A2 - Émission de cycle initiée par coupure et réaction de remplacement - Google Patents

Émission de cycle initiée par coupure et réaction de remplacement

Info

Publication number
EP4731784A2
EP4731784A2 EP24826844.3A EP24826844A EP4731784A2 EP 4731784 A2 EP4731784 A2 EP 4731784A2 EP 24826844 A EP24826844 A EP 24826844A EP 4731784 A2 EP4731784 A2 EP 4731784A2
Authority
EP
European Patent Office
Prior art keywords
sequence
probe
stranded
hairpin
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24826844.3A
Other languages
German (de)
English (en)
Inventor
Joseph Dunham
Benjamin AKINS
Arnel Arnold Santos GANCENIA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seqonce Biosciences Inc
Original Assignee
Seqonce Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seqonce Biosciences Inc filed Critical Seqonce Biosciences Inc
Publication of EP4731784A2 publication Critical patent/EP4731784A2/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente divulgation concerne des détails de procédés isothermes sans polymérase pour détecter des séquences nucléotidiques. Des procédés utilisent des oligonucléotides en épingle à cheveux spécifiques à une cible, qui interagissent également particulièrement avec des sondes marquées par des fractions de colorant fluorescent et un extincteur de fluorescence, pour amplifier la détection de polynucléotides cibles par répétition de cycles de coupure à médiation par endonucléase des sondes pour provoquer la dénaturation de fragments de sonde marquée par fluorophore à l'opposé des complexes en épingle à cheveux-sonde pour dissocier les marqueurs fluorescents des extincteurs de fluorescence.
EP24826844.3A 2023-06-23 2024-06-24 Émission de cycle initiée par coupure et réaction de remplacement Pending EP4731784A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363509990P 2023-06-23 2023-06-23
PCT/US2024/035320 WO2024264074A2 (fr) 2023-06-23 2024-06-24 Émission de cycle initiée par coupure et réaction de remplacement

Publications (1)

Publication Number Publication Date
EP4731784A2 true EP4731784A2 (fr) 2026-04-29

Family

ID=93936355

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24826844.3A Pending EP4731784A2 (fr) 2023-06-23 2024-06-24 Émission de cycle initiée par coupure et réaction de remplacement

Country Status (2)

Country Link
EP (1) EP4731784A2 (fr)
WO (1) WO2024264074A2 (fr)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7169561B2 (en) * 2004-12-29 2007-01-30 Applera Corporation Methods, compositions, and kits for forming self-complementary polynucleotides
CN102292457B (zh) * 2008-11-25 2014-04-09 简·探针公司 检测小rna的组合物和方法及其用途
WO2021003410A1 (fr) * 2019-07-03 2021-01-07 Napigen, Inc. Modification du génome d'organites
EP4428244B1 (fr) * 2020-06-24 2025-10-15 Claret Bioscience, LLC Procédés et compositions pour analyser un acide nucléique
US20220403458A1 (en) * 2021-06-21 2022-12-22 10X Genomics, Inc. Methods to generate circularizable probes in situ

Also Published As

Publication number Publication date
WO2024264074A2 (fr) 2024-12-26
WO2024264074A3 (fr) 2025-05-08

Similar Documents

Publication Publication Date Title
JP5027126B2 (ja) リコンビナーゼポリメラーゼ増幅を多重化するための方法
JP2025026849A (ja) 核酸検出方法
CN107760770B (zh) 用于核酸合成和扩增的组合物、方法和试剂盒
US20060292616A1 (en) Single molecule miRNA-based disease diagnostic methods
JP6641397B2 (ja) 核酸酵素基質
US20120322067A1 (en) Assay method for target nucleic acid by signal amplification using probe hybridization and restriction
CN106434857A (zh) 靶区分性探针及其用途
US20080081335A1 (en) Oligonucleotide probe/primer methods for polynucleotide detection
JP6876437B2 (ja) 鎖侵入に基づくｄｎａ増幅法
JP2023518217A (ja) 標的核酸を検出するためのループプライマー及びループ・デ・ループ方法
WO2014152185A1 (fr) Détection d'allèles multiplex
JP2008529528A (ja) ポリヌクレオチドの検出用のキープローブ組成物および方法
WO2021091487A1 (fr) Procédé de détection d'acide nucléique à l'aide de la technique lamp et une détection de sonde
CN108588279A (zh) Hev测定
CN109680044B (zh) 一种基于选择性消除野生链背景干扰的基因突变检测方法
WO2006045009A2 (fr) Compositions de sonde triplex et procedes de detection de polynucleotide
EP4731784A2 (fr) Émission de cycle initiée par coupure et réaction de remplacement
KR102678676B1 (ko) 인공핵산을 이용한 표적핵산의 검출 방법
KR20220131925A (ko) 바이러스 검출
WO2024003792A1 (fr) Dosages de détection virale
JPWO2007111324A1 (ja) 遺伝子中の５−メチルシトシン検出方法および検出キット
US20070184453A1 (en) Fret process
WO2026050332A1 (fr) Procédés et compositions pour amplifier et/ou détecter des acides nucléiques de rotavirus
EP4426854A1 (fr) Compositions et procédés pour réduire la contamination de mélange maître
WO2025078657A1 (fr) Flux de travail d'enrichissement cible sans amplification pour détection directe de modifications d'acide nucléique

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20260123

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR