EP4731784A2 - Nick-initiierte radfahremission und ersatzreaktion - Google Patents

Nick-initiierte radfahremission und ersatzreaktion

Info

Publication number
EP4731784A2
EP4731784A2 EP24826844.3A EP24826844A EP4731784A2 EP 4731784 A2 EP4731784 A2 EP 4731784A2 EP 24826844 A EP24826844 A EP 24826844A EP 4731784 A2 EP4731784 A2 EP 4731784A2
Authority
EP
European Patent Office
Prior art keywords
sequence
probe
stranded
hairpin
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24826844.3A
Other languages
English (en)
French (fr)
Inventor
Joseph Dunham
Benjamin AKINS
Arnel Arnold Santos GANCENIA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seqonce Biosciences Inc
Original Assignee
Seqonce Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seqonce Biosciences Inc filed Critical Seqonce Biosciences Inc
Publication of EP4731784A2 publication Critical patent/EP4731784A2/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP24826844.3A 2023-06-23 2024-06-24 Nick-initiierte radfahremission und ersatzreaktion Pending EP4731784A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363509990P 2023-06-23 2023-06-23
PCT/US2024/035320 WO2024264074A2 (en) 2023-06-23 2024-06-24 Nick initiated cycling emission and replacement reaction

Publications (1)

Publication Number Publication Date
EP4731784A2 true EP4731784A2 (de) 2026-04-29

Family

ID=93936355

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24826844.3A Pending EP4731784A2 (de) 2023-06-23 2024-06-24 Nick-initiierte radfahremission und ersatzreaktion

Country Status (3)

Country Link
EP (1) EP4731784A2 (de)
CN (1) CN122161944A (de)
WO (1) WO2024264074A2 (de)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE457363T1 (de) * 2004-12-29 2010-02-15 Applied Biosystems Llc Verfahren, zusammensetzungen und kits zur bildung einander ergänzender polynukleotide
JP2012509675A (ja) * 2008-11-25 2012-04-26 ジェン−プロウブ インコーポレイテッド 低分子rnaを検出するための組成物および方法、ならびにその使用
WO2021003410A1 (en) * 2019-07-03 2021-01-07 Napigen, Inc. Organelle genome modification
DK4172357T3 (da) * 2020-06-24 2024-08-05 Claret Bioscience Llc Fremgangsmåder og sammensætninger til analyse af nucleinsyrer
US20220403458A1 (en) * 2021-06-21 2022-12-22 10X Genomics, Inc. Methods to generate circularizable probes in situ

Also Published As

Publication number Publication date
WO2024264074A3 (en) 2025-05-08
WO2024264074A2 (en) 2024-12-26
CN122161944A (zh) 2026-06-05

Similar Documents

Publication Publication Date Title
JP7210203B2 (ja) リコンビナーゼポリメラーゼ増幅を多重化するための方法
JP2025026849A (ja) 核酸検出方法
CN107760770B (zh) 用于核酸合成和扩增的组合物、方法和试剂盒
US8916349B2 (en) Assay method for target nucleic acid by signal amplification using probe hybridization and restriction
US20060292616A1 (en) Single molecule miRNA-based disease diagnostic methods
JP5385973B2 (ja) 反応における複数の核酸配列の同時検出
JP6641397B2 (ja) 核酸酵素基質
JP6876437B2 (ja) 鎖侵入に基づくdna増幅法
CN106434857A (zh) 靶区分性探针及其用途
US20080081335A1 (en) Oligonucleotide probe/primer methods for polynucleotide detection
JP2023518217A (ja) 標的核酸を検出するためのループプライマー及びループ・デ・ループ方法
EP2971147A1 (de) Multiplex-allelnachweis
JP2008529528A (ja) ポリヌクレオチドの検出用のキープローブ組成物および方法
WO2021091487A1 (en) Nucleic acid detection method using lamp and probe detection
CN109680044B (zh) 一种基于选择性消除野生链背景干扰的基因突变检测方法
CN108588279A (zh) Hev测定
WO2006045009A2 (en) Triplex probe compositions and methods for polynucleotide detection
WO2024264074A2 (en) Nick initiated cycling emission and replacement reaction
KR102678676B1 (ko) 인공핵산을 이용한 표적핵산의 검출 방법
KR20220131925A (ko) 바이러스 검출
JP4238381B2 (ja) ピレン修飾rna、およびrnaの分析方法
WO2024003792A1 (en) Viral detection assays
CN114807315A (zh) Ψ型杂交和Cas12a辅助的信号放大检测microRNA的方法及应用
JPWO2007111324A1 (ja) 遺伝子中の5−メチルシトシン検出方法および検出キット
WO2026050332A1 (en) Methods and compositions for amplifying and/or detecting rotavirus nucleic acids

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20260123

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR