EP4735580A1 - Regulierung des zellstoffwechsels von zellen durch lipoatproteinligase und ihre verwendungen - Google Patents

Regulierung des zellstoffwechsels von zellen durch lipoatproteinligase und ihre verwendungen

Info

Publication number
EP4735580A1
EP4735580A1 EP24831010.4A EP24831010A EP4735580A1 EP 4735580 A1 EP4735580 A1 EP 4735580A1 EP 24831010 A EP24831010 A EP 24831010A EP 4735580 A1 EP4735580 A1 EP 4735580A1
Authority
EP
European Patent Office
Prior art keywords
cell
host cell
cells
interest
protein ligase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24831010.4A
Other languages
English (en)
French (fr)
Inventor
Anping ZENG
Zhijuan HU
Peiguo YANG
Runqing YANG
Yingying Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Westlake University
Original Assignee
Westlake University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Westlake University filed Critical Westlake University
Publication of EP4735580A1 publication Critical patent/EP4735580A1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/89—Algae ; Processes using algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP24831010.4A 2023-06-28 2024-06-28 Regulierung des zellstoffwechsels von zellen durch lipoatproteinligase und ihre verwendungen Pending EP4735580A1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN2023103401 2023-06-28
PCT/CN2024/102481 WO2025002383A1 (en) 2023-06-28 2024-06-28 Regulation of cellular energy metabolism by lipoate protein ligase and its applications

Publications (1)

Publication Number Publication Date
EP4735580A1 true EP4735580A1 (de) 2026-05-06

Family

ID=93937700

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24831010.4A Pending EP4735580A1 (de) 2023-06-28 2024-06-28 Regulierung des zellstoffwechsels von zellen durch lipoatproteinligase und ihre verwendungen

Country Status (3)

Country Link
EP (1) EP4735580A1 (de)
CN (1) CN121488029A (de)
WO (1) WO2025002383A1 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120796161B (zh) * 2025-09-09 2026-01-09 西湖大学 一种基因改造的链霉菌、提升次级代谢产物产量的链霉菌发酵方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005514915A (ja) * 2001-09-28 2005-05-26 セレクテックス ファーマシューティカルズ インコーポレーティッド 標的分子と結合する化合物の生成、選択および同定のための方法
DE10245993A1 (de) * 2002-10-02 2004-05-06 Consortium für elektrochemische Industrie GmbH Zellen zur fermentativen Herstellung von R-alpha-Liponsäure
CN101578361A (zh) * 2005-06-17 2009-11-11 米克罗比亚精密工程股份有限公司 改进的氨基酸和代谢物生物合成
WO2008101857A2 (en) * 2007-02-19 2008-08-28 Evonik Degussa Gmbh Coryneform bacteria with formate-thf-synthetase and/or glycine cleavage activity
EP3058059A4 (de) * 2013-10-18 2017-09-27 Biopetrolia AB Herstellung von acetyl-coa-metabolismus in hefe
WO2015084633A1 (en) * 2013-12-03 2015-06-11 Genomatica, Inc. Microorganisms and methods for improving product yields on methanol using acetyl-coa synthesis
CN106574254A (zh) * 2014-04-30 2017-04-19 赢创德固赛有限公司 使用嗜碱性细菌生产l‑氨基酸的方法
WO2017106747A1 (en) * 2015-12-16 2017-06-22 Washington University Host cells and methods for producing fatty acid-derivatives with high branched-chain percentage
CN112168808B (zh) * 2019-07-02 2023-01-24 深圳市第二人民医院 一种基于crispr的细胞核靶向药物递送系统

Also Published As

Publication number Publication date
CN121488029A (zh) 2026-02-06
WO2025002383A1 (en) 2025-01-02

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