EP4735580A1 - Régulation du métabolisme de l'énergie cellulaire par la lipoate-protéine ligase et ses applications - Google Patents

Régulation du métabolisme de l'énergie cellulaire par la lipoate-protéine ligase et ses applications

Info

Publication number
EP4735580A1
EP4735580A1 EP24831010.4A EP24831010A EP4735580A1 EP 4735580 A1 EP4735580 A1 EP 4735580A1 EP 24831010 A EP24831010 A EP 24831010A EP 4735580 A1 EP4735580 A1 EP 4735580A1
Authority
EP
European Patent Office
Prior art keywords
cell
host cell
cells
interest
protein ligase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24831010.4A
Other languages
German (de)
English (en)
Inventor
Anping ZENG
Zhijuan HU
Peiguo YANG
Runqing YANG
Yingying Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Westlake University
Original Assignee
Westlake University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Westlake University filed Critical Westlake University
Publication of EP4735580A1 publication Critical patent/EP4735580A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/89—Algae ; Processes using algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des procédés de régulation du métabolisme énergétique cellulaire par la lipoate-protéine ligase, la croissance cellulaire ou la production de molécules d'intérêt dans une cellule étant améliorée par surexpression d'une lipoate-protéine ligase dans la cellule.
EP24831010.4A 2023-06-28 2024-06-28 Régulation du métabolisme de l'énergie cellulaire par la lipoate-protéine ligase et ses applications Pending EP4735580A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN2023103401 2023-06-28
PCT/CN2024/102481 WO2025002383A1 (fr) 2023-06-28 2024-06-28 Régulation du métabolisme de l'énergie cellulaire par la lipoate-protéine ligase et ses applications

Publications (1)

Publication Number Publication Date
EP4735580A1 true EP4735580A1 (fr) 2026-05-06

Family

ID=93937700

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24831010.4A Pending EP4735580A1 (fr) 2023-06-28 2024-06-28 Régulation du métabolisme de l'énergie cellulaire par la lipoate-protéine ligase et ses applications

Country Status (3)

Country Link
EP (1) EP4735580A1 (fr)
CN (1) CN121488029A (fr)
WO (1) WO2025002383A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120796161B (zh) * 2025-09-09 2026-01-09 西湖大学 一种基因改造的链霉菌、提升次级代谢产物产量的链霉菌发酵方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005514915A (ja) * 2001-09-28 2005-05-26 セレクテックス ファーマシューティカルズ インコーポレーティッド 標的分子と結合する化合物の生成、選択および同定のための方法
DE10245993A1 (de) * 2002-10-02 2004-05-06 Consortium für elektrochemische Industrie GmbH Zellen zur fermentativen Herstellung von R-alpha-Liponsäure
CN101578361A (zh) * 2005-06-17 2009-11-11 米克罗比亚精密工程股份有限公司 改进的氨基酸和代谢物生物合成
WO2008101857A2 (fr) * 2007-02-19 2008-08-28 Evonik Degussa Gmbh Bactérie de type corynéen avec activité formiate-thf-synthétase et/ou clivage de glycocolle
EP3058059A4 (fr) * 2013-10-18 2017-09-27 Biopetrolia AB Modification du métabolisme de l'acétyl-coa dans la levure
WO2015084633A1 (fr) * 2013-12-03 2015-06-11 Genomatica, Inc. Microorganismes et procédés pour améliorer les rendements de produits sur le méthanol faisant appel à la synthèse de l'acétyl-coa
CN106574254A (zh) * 2014-04-30 2017-04-19 赢创德固赛有限公司 使用嗜碱性细菌生产l‑氨基酸的方法
WO2017106747A1 (fr) * 2015-12-16 2017-06-22 Washington University Cellules hôtes et procédés de production de dérivés d'acides gras ayant un pourcentage de chaîne ramifiée élevé
CN112168808B (zh) * 2019-07-02 2023-01-24 深圳市第二人民医院 一种基于crispr的细胞核靶向药物递送系统

Also Published As

Publication number Publication date
CN121488029A (zh) 2026-02-06
WO2025002383A1 (fr) 2025-01-02

Similar Documents

Publication Publication Date Title
KR102022662B1 (ko) 식물 세포에서의 목적 단백질 발현을 위한 재조합 벡터
JP6793957B2 (ja) 改変された全細胞触媒を用いたノンカロリー甘味料の生成
CA2778150C (fr) Proteines d'encapsulation d'huile modifiee et utilisations de celles-ci
Sasaki et al. Two members of the aluminum-activated malate transporter family, SlALMT4 and SlALMT5, are expressed during fruit development, and the overexpression of SlALMT5 alters organic acid contents in seeds in tomato (Solanum lycopersicum)
CA2764589C (fr) Gene capable d'ameliorer la productivite d'une substance dans une semence, et son procede d'utilisation
KR20200012956A (ko) 합성 캘빈 사이클을 발현하는 효모
WO2025002383A1 (fr) Régulation du métabolisme de l'énergie cellulaire par la lipoate-protéine ligase et ses applications
Campos-Quevedo et al. Production of milk-derived bioactive peptides as precursor chimeric proteins in chloroplasts of Chlamydomonas reinhardtii
Bi et al. Identification, functional study, and promoter analysis of HbMFT1, a homolog of MFT from rubber tree (Hevea brasiliensis)
CN113631567A (zh) 在发酵方法中释放重组蛋白的细菌菌株
US11773399B2 (en) Methods and compositions for enhancing polypeptide production
US20210403959A1 (en) Use of type i and type ii polyketide synthases for the production of cannabinoids and cannabinoid analogs
KR101957561B1 (ko) RbcS 융합 단백질을 이용하여 식물로부터 목적 단백질을　고발현하는 방법 및 목적 단백질 발현 식물체를 이용한 의료용 단백질의 경구투여용 조성물의 제조 방법
Li et al. Specialized endoplasmic reticulum‐derived vesicles in plants: Functional diversity, evolution, and biotechnological exploitation
Karki et al. High yield secretion of human erythropoietin from tobacco cells for ex vivo differentiation of hematopoietic stem cells towards red blood cells
CN113980932B (zh) 一种定点突变的α-葡萄糖苷酶
CN102533683A (zh) 毛竹紫黄质脱环氧化酶PeVDE蛋白，编码该蛋白的基因和应用
JP2010183904A (ja) 組換え植物で発現して難抽出化した組換えタンパク質の抽出・精製方法
KR101788038B1 (ko) 천연고무 중합효소 유전자 및 이의 용도
JP7667540B2 (ja) 植物を用いた塩基性線維芽細胞増殖因子の製造方法
KR101449846B1 (ko) Ｍａｘ２－ｌｒｒ 도메인 단백질 또는 이를 코딩하는 유전자를 포함하는 식물체의 유효 번식성 줄기 형성 촉진용 조성물
KR20130015819A (ko) 카로티노이드 및 안토시아닌 고축적 형질전환 고구마 식물체의 제조 방법 및 그에 따른 식물체
US20220154197A1 (en) Cannabinoid precursor production
JP2022114271A (ja) 植物及び９，１０－α－ケトールリノレン酸の製造方法
KR101625746B1 (ko) 프로미니인슐린 재조합 단백질이 발현된 형질전환 식물 및 이의 제조방법

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE