EP4771391A1 - Verfahren und zusammensetzungen zur barcodierung von cdna - Google Patents
Verfahren und zusammensetzungen zur barcodierung von cdnaInfo
- Publication number
- EP4771391A1 EP4771391A1 EP24861222.8A EP24861222A EP4771391A1 EP 4771391 A1 EP4771391 A1 EP 4771391A1 EP 24861222 A EP24861222 A EP 24861222A EP 4771391 A1 EP4771391 A1 EP 4771391A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- sequence
- partitions
- gene
- tail
- bead
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202363535513P | 2023-08-30 | 2023-08-30 | |
| PCT/US2024/044859 WO2025050036A1 (en) | 2023-08-30 | 2024-08-30 | Methods and compositions for barcoding cdna |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP4771391A1 true EP4771391A1 (de) | 2026-07-08 |
Family
ID=94820497
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP24861222.8A Pending EP4771391A1 (de) | 2023-08-30 | 2024-08-30 | Verfahren und zusammensetzungen zur barcodierung von cdna |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP4771391A1 (de) |
| CN (1) | CN121773332A (de) |
| WO (1) | WO2025050036A1 (de) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3746552B1 (de) * | 2018-01-31 | 2023-12-27 | Bio-Rad Laboratories, Inc. | Verfahren und zusammensetzungen zur dekonvolution von partitionsbarcodes |
| CN113454234B (zh) * | 2019-02-14 | 2025-03-18 | 贝克顿迪金森公司 | 杂合体靶向和全转录物组扩增 |
| US20230143569A1 (en) * | 2019-02-28 | 2023-05-11 | 10X Genomics, Inc. | Profiling of biological analytes with spatially barcoded oligonucleotide arrays |
| WO2023059917A2 (en) * | 2021-10-08 | 2023-04-13 | Bio-Rad Laboratories, Inc. | B(ead-based) a(tacseq) p(rocessing) |
| WO2024086217A2 (en) * | 2022-10-20 | 2024-04-25 | Bio-Rad Laboratories, Inc. | Methods and compositions for tracking barcodes in partitions |
-
2024
- 2024-08-30 EP EP24861222.8A patent/EP4771391A1/de active Pending
- 2024-08-30 CN CN202480055553.9A patent/CN121773332A/zh active Pending
- 2024-08-30 WO PCT/US2024/044859 patent/WO2025050036A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2025050036A1 (en) | 2025-03-06 |
| CN121773332A (zh) | 2026-03-31 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
|
| 17P | Request for examination filed |
Effective date: 20260226 |
|
| AK | Designated contracting states |
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