EP4771391A1 - Procédés et compositions pour le codage à barres d'adnc - Google Patents

Procédés et compositions pour le codage à barres d'adnc

Info

Publication number
EP4771391A1
EP4771391A1 EP24861222.8A EP24861222A EP4771391A1 EP 4771391 A1 EP4771391 A1 EP 4771391A1 EP 24861222 A EP24861222 A EP 24861222A EP 4771391 A1 EP4771391 A1 EP 4771391A1
Authority
EP
European Patent Office
Prior art keywords
sequence
partitions
gene
tail
bead
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24861222.8A
Other languages
German (de)
English (en)
Inventor
Eric K. POMARANSKI
Adam M. Mccoy
Jeffrey W. Perry
Niloufar FATTAHI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of EP4771391A1 publication Critical patent/EP4771391A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés et des compositions qui permettent de préparer des ARN, à partir d'une cellule unique, pour des applications de séquençage. Des oligonucléotides de codage à barres spécifiques à des billes sont utilisés pour introduire une séquence de code à barres spécifique à des billes, une séquence d'adaptateur universelle, et éventuellement une ou plusieurs séquences d'extrémité à l'ADNc provenant de la cellule unique. L'invention concerne également une pluralité de partitions qui comprennent une cellule unique ou un noyau ou des acides nucléiques provenant d'une cellule ou d'un noyau unique, des billes comprenant des oligonucléotides de codage à barres spécifiques à des billes, un oligonucléotide de détection, une transcriptase inverse et une ADN polymérase.
EP24861222.8A 2023-08-30 2024-08-30 Procédés et compositions pour le codage à barres d'adnc Pending EP4771391A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363535513P 2023-08-30 2023-08-30
PCT/US2024/044859 WO2025050036A1 (fr) 2023-08-30 2024-08-30 Procédés et compositions pour le codage à barres d'adnc

Publications (1)

Publication Number Publication Date
EP4771391A1 true EP4771391A1 (fr) 2026-07-08

Family

ID=94820497

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24861222.8A Pending EP4771391A1 (fr) 2023-08-30 2024-08-30 Procédés et compositions pour le codage à barres d'adnc

Country Status (3)

Country Link
EP (1) EP4771391A1 (fr)
CN (1) CN121773332A (fr)
WO (1) WO2025050036A1 (fr)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3746552B1 (fr) * 2018-01-31 2023-12-27 Bio-Rad Laboratories, Inc. Procédés et compositions pour déconvolutionner des codes barres de séparation
CN113454234B (zh) * 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
US20230143569A1 (en) * 2019-02-28 2023-05-11 10X Genomics, Inc. Profiling of biological analytes with spatially barcoded oligonucleotide arrays
WO2023059917A2 (fr) * 2021-10-08 2023-04-13 Bio-Rad Laboratories, Inc. Traitement atacseq à base de billes
WO2024086217A2 (fr) * 2022-10-20 2024-04-25 Bio-Rad Laboratories, Inc. Procédés et compositions pour suivre des codes-barres dans des partitions

Also Published As

Publication number Publication date
WO2025050036A1 (fr) 2025-03-06
CN121773332A (zh) 2026-03-31

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