ES2006382A6 - Procedimiento para la preparacion de un polipeptido mediante levadura que contiene un promotor hibrido con los puntos de activacion aguas arriba del gen pho5 de levadura. - Google Patents

Procedimiento para la preparacion de un polipeptido mediante levadura que contiene un promotor hibrido con los puntos de activacion aguas arriba del gen pho5 de levadura.

Info

Publication number
ES2006382A6
ES2006382A6 ES8800794A ES8800794A ES2006382A6 ES 2006382 A6 ES2006382 A6 ES 2006382A6 ES 8800794 A ES8800794 A ES 8800794A ES 8800794 A ES8800794 A ES 8800794A ES 2006382 A6 ES2006382 A6 ES 2006382A6
Authority
ES
Spain
Prior art keywords
yeast
polypeptide
gene
promoter
hybrid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8800794A
Other languages
English (en)
Inventor
Albert Hinnen
Bernd Meyhack
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Ciba Geigy AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy AG filed Critical Ciba Geigy AG
Publication of ES2006382A6 publication Critical patent/ES2006382A6/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Alcoholic Beverages (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Graft Or Block Polymers (AREA)
  • Led Devices (AREA)
  • Gyroscopes (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

PROCEDIMIENTO PARA LA PREPARACION DE UN POLIPEPTIDO MEDIANTE LEVADURA QUE CONTIENE UN PROMOTOR HIBRIDO CON LOS PUNTOS DE ACTIVACION AGUAS ARRIBA DEL GEN PH05 DE LEVADURA, CARACTERIZADO PORQUE SE CULTIVA LA CEPA DE LEVADURA TRANSFORMADA CON VECTOR HIBRIDO QUE CONTIENE UNO O MULTIPLES INSERTOS DE DNA, COMPRENDIENDO CADA UNO DE ELLOS UN SEGMENTO DE DNA QUE CODIFICA UN POLIPEPTIDO HETEROLOGO A LEVADURA, BAJO EL CONTROL TRANSCRIPCIONAL DE UN PROMOTOR HIBRIDO CONSISTENTE EN UN ELEMENTO PROMOTOR AGUAS ARRIBA 5'' CON UAS(S) DEL GEN PH05 DE LEVADURA Y UN ELEMENTO PROMOTOR AGUAS ABAJO 3'' DE UN GEN DE LEVADURA DISTINTO DEL GEN PH05 QUE COMPRENDE PUNTOS DE INICIACION DE LA TRANSCRIPCION INCLUYENDO UNA CAJA TATA FUNCIONAL, O UN MUTANTE DEL MISMO; Y SE AISLA EL POLIPEPTIDO ASI EXPRESADO.
ES8800794A 1985-08-29 1988-03-16 Procedimiento para la preparacion de un polipeptido mediante levadura que contiene un promotor hibrido con los puntos de activacion aguas arriba del gen pho5 de levadura. Expired ES2006382A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB858521496A GB8521496D0 (en) 1985-08-29 1985-08-29 Repressible yeast promoters

Publications (1)

Publication Number Publication Date
ES2006382A6 true ES2006382A6 (es) 1989-04-16

Family

ID=10584428

Family Applications (3)

Application Number Title Priority Date Filing Date
ES8601490A Expired ES2001618A6 (es) 1985-08-29 1986-08-29 Procedimiento para la preparacion de promotores de levadura represibles conteniendo puntos de actividad agua arriba del gen phos de levadura.
ES8800795A Expired ES2006383A6 (es) 1985-08-29 1988-03-16 Procedimiento para la preparacion de vectores hibridos de levadura comprendiendo promotores de levadura represibles con los lugares de actuacion aguas arriba del gen ph05 de levadura.
ES8800794A Expired ES2006382A6 (es) 1985-08-29 1988-03-16 Procedimiento para la preparacion de un polipeptido mediante levadura que contiene un promotor hibrido con los puntos de activacion aguas arriba del gen pho5 de levadura.

Family Applications Before (2)

Application Number Title Priority Date Filing Date
ES8601490A Expired ES2001618A6 (es) 1985-08-29 1986-08-29 Procedimiento para la preparacion de promotores de levadura represibles conteniendo puntos de actividad agua arriba del gen phos de levadura.
ES8800795A Expired ES2006383A6 (es) 1985-08-29 1988-03-16 Procedimiento para la preparacion de vectores hibridos de levadura comprendiendo promotores de levadura represibles con los lugares de actuacion aguas arriba del gen ph05 de levadura.

Country Status (22)

Country Link
US (1) US5436136A (es)
EP (1) EP0213593B1 (es)
JP (1) JPH07110233B2 (es)
AT (1) ATE62510T1 (es)
AU (1) AU599329B2 (es)
CA (1) CA1318616C (es)
DD (2) DD254212A5 (es)
DE (1) DE3678647D1 (es)
DK (1) DK175093B1 (es)
ES (3) ES2001618A6 (es)
FI (1) FI91280C (es)
GB (1) GB8521496D0 (es)
GR (1) GR862209B (es)
HU (1) HU211207B (es)
IE (1) IE58844B1 (es)
IL (1) IL79837A (es)
NO (1) NO175871C (es)
NZ (1) NZ217388A (es)
PH (1) PH24715A (es)
PT (1) PT83273B (es)
SU (1) SU1630616A3 (es)
ZA (1) ZA866535B (es)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0245479B1 (en) * 1985-11-13 1993-02-17 Xoma Corporation Shortened phosphoglycerate kinase promoter
MY101203A (en) * 1985-12-12 1991-08-17 Ucp Gen Pharma Ag Production of thrombin iinhibitors.
GB8530631D0 (en) 1985-12-12 1986-01-22 Ciba Geigy Ag Thrombin inhibitors
GB8620926D0 (en) * 1986-08-29 1986-10-08 Delta Biotechnology Ltd Yeast promoter
GB8907110D0 (en) * 1988-05-04 1989-05-10 Ciba Geigy Ag Improvements in the production of polypeptides
NO177065C (no) * 1988-09-26 1995-07-12 Labofina Sa Framgangsmåte for framstilling av enzymatisk aktivt humant lysozym
EP0548267A4 (en) * 1990-09-04 1994-11-23 Salk Inst Biotech Ind Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
ATE220105T1 (de) * 1991-04-01 2002-07-15 Merck & Co Inc Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung
US5674728A (en) * 1993-11-03 1997-10-07 Novartis Corporation Aspergillus niger vacuolar aspartyl protease
JPH08173170A (ja) * 1994-05-25 1996-07-09 Kirin Brewery Co Ltd キャンディダ・ユティリス酵母の形質転換系、およびそれによる異種遺伝子の発現
AU733007B2 (en) * 1996-09-24 2001-05-03 Cadus Pharmaceutical Corporation Methods and compositions for identifying receptor effectors
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
US6890730B1 (en) 1999-12-10 2005-05-10 The Salk Institute Sequence and method for increasing protein expression in cellular expression systems
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
WO2011146833A1 (en) 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
NZ727213A (en) 2012-03-09 2020-03-27 Vestaron Corp Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
JP2023522947A (ja) 2020-04-20 2023-06-01 ヴェスタロン・コーポレーション タンパク質分解に対して安定な害虫制御用ｕ１－アガトキシン－ｔａ１ｂバリアントポリペプチド
IL297738A (en) 2020-05-01 2022-12-01 Vestaron Corp Insecticidal combinations
US20250188136A1 (en) 2021-04-01 2025-06-12 Vestaron Corporation Av3 mutant polypeptides for pest control
WO2023122805A1 (en) 2021-12-20 2023-06-29 Vestaron Corporation Sorbitol driven selection pressure method
US20260013513A1 (en) 2022-05-18 2026-01-15 Vestaron Corporation Pesticidal actx peptide variants
WO2023245100A1 (en) 2022-06-17 2023-12-21 Vestaron Corporation Antimicrobial ncr13 variant peptides
WO2024026406A2 (en) 2022-07-29 2024-02-01 Vestaron Corporation Next Generation ACTX Peptides
WO2024259306A2 (en) 2023-06-16 2024-12-19 Vestaron Corporation Pvd1 variant polypeptides for pest control
WO2025226701A1 (en) 2024-04-23 2025-10-30 Vestaron Corporation Proteolytically stable u1-agatoxin-ta1b variant polypeptides for pest control

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
AU577259B2 (en) * 1982-08-13 1988-09-22 Zymogenetics Inc. Glycolytic promters for regulated protein expression protease inhibitor
US4876197A (en) * 1983-02-22 1989-10-24 Chiron Corporation Eukaryotic regulatable transcription
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
EP0127839B1 (en) * 1983-05-27 1992-07-15 THE TEXAS A&M UNIVERSITY SYSTEM Method for producing a recombinant baculovirus expression vector
GB8314961D0 (en) * 1983-05-31 1983-07-06 Kingsman A J Dna sequence
DE3479520D1 (en) * 1983-11-21 1989-09-28 Ciba Geigy Ag Synthesis of tissue plasminogen activator(tpa) by yeast
ATE102250T1 (de) * 1984-05-11 1994-03-15 Chiron Corp Erhoehte hefetranskription unter verwendung einer hybridkonstruktion der promotorregion.
EP0164566A3 (de) * 1984-05-11 1986-10-29 LGA Gastechnik GmbH Sicherheitseinrichtung für Flüssiggastanks
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen

Also Published As

Publication number Publication date
DD267739A5 (de) 1989-05-10
DK175093B1 (da) 2004-05-24
GR862209B (en) 1986-12-31
ATE62510T1 (de) 1991-04-15
CA1318616C (en) 1993-06-01
NO863458D0 (no) 1986-08-28
DK409686A (da) 1987-03-01
FI863430L (fi) 1987-03-01
JPS6251995A (ja) 1987-03-06
AU599329B2 (en) 1990-07-19
DK409686D0 (da) 1986-08-28
GB8521496D0 (en) 1985-10-02
HU211207B (en) 1995-11-28
DD254212A5 (de) 1988-02-17
PT83273A (en) 1986-09-01
FI91280C (fi) 1994-06-10
IE862307L (en) 1987-02-28
EP0213593B1 (en) 1991-04-10
NO175871B (es) 1994-09-12
SU1630616A3 (ru) 1991-02-23
IL79837A (en) 1992-06-21
NO863458L (no) 1987-03-02
ZA866535B (en) 1987-04-29
PT83273B (pt) 1989-05-12
HUT42525A (en) 1987-07-28
US5436136A (en) 1995-07-25
ES2006383A6 (es) 1989-04-16
ES2001618A6 (es) 1988-06-01
PH24715A (en) 1990-10-01
NZ217388A (en) 1989-02-24
DE3678647D1 (en) 1991-05-16
FI863430A0 (fi) 1986-08-25
FI91280B (fi) 1994-02-28
IL79837A0 (en) 1986-11-30
JPH07110233B2 (ja) 1995-11-29
NO175871C (no) 1994-12-21
AU6203286A (en) 1987-03-05
EP0213593A1 (en) 1987-03-11
IE58844B1 (en) 1993-11-17

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Legal Events

Date Code Title Description
PC1A Transfer granted
FD1A Patent lapsed

Effective date: 20060830