ES2009599A6 - Un metodo para producir la toxina p-2 de bacillus thuringiensis. - Google Patents

Un metodo para producir la toxina p-2 de bacillus thuringiensis.

Info

Publication number
ES2009599A6
ES2009599A6 ES8801170A ES8801170A ES2009599A6 ES 2009599 A6 ES2009599 A6 ES 2009599A6 ES 8801170 A ES8801170 A ES 8801170A ES 8801170 A ES8801170 A ES 8801170A ES 2009599 A6 ES2009599 A6 ES 2009599A6
Authority
ES
Spain
Prior art keywords
toxin
protein
bacillus thuringiensis
bacillus
toxin gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8801170A
Other languages
English (en)
Inventor
William Preston Donovan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ecogen Inc
Original Assignee
Ecogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecogen Inc filed Critical Ecogen Inc
Publication of ES2009599A6 publication Critical patent/ES2009599A6/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A01N63/23—B. thuringiensis
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pest Control & Pesticides (AREA)
  • Physics & Mathematics (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Insects & Arthropods (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

METODO PARA PRODUCIR LA TOXINA P-2 DE BACILLUS THURINGIENSIS, EN EL QUE SE TRANSFORMA UN MICROORGANISMO SELECCIONADO ENTRE BACILLUS, ESCHERICHIA Y CYANOBACTERIA, PREFERIBLEMENTE ESCHERICHIA COLI NRRL B-18204 Y BACILLUS MEGATORIUM NRRL B-18203, CON UN PLASMIDO PORTADOR DEL GEN QUE CODIFICA LA TOXINA, Y SE CULTIVA EL MICROORGANISMO ASI TRANSFORMADO EN CONDICIONES QUE PERMITAN LA EXPRESION DEL GEN Y LA PRODUCCION DE LA TOXINA P-2 DESEADA. LA TOXINA PROTEINICA P-2 OBTENIDA ES UTIL PARA CONTROLAR INFESTACIONES DE LEPIDOPTEROS Y DIPTEROS EN PLANTAS.
ES8801170A 1987-04-16 1988-04-15 Un metodo para producir la toxina p-2 de bacillus thuringiensis. Expired ES2009599A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US3954287A 1987-04-16 1987-04-16

Publications (1)

Publication Number Publication Date
ES2009599A6 true ES2009599A6 (es) 1989-10-01

Family

ID=21906033

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8801170A Expired ES2009599A6 (es) 1987-04-16 1988-04-15 Un metodo para producir la toxina p-2 de bacillus thuringiensis.

Country Status (14)

Country Link
EP (1) EP0367767B1 (es)
JP (1) JPH0636743B2 (es)
KR (1) KR960006582B1 (es)
AT (1) ATE150089T1 (es)
AU (1) AU618532B2 (es)
CA (1) CA1340600C (es)
DE (1) DE3855827T2 (es)
DK (1) DK513689A (es)
ES (1) ES2009599A6 (es)
FI (1) FI894856A7 (es)
GR (1) GR1000679B (es)
IL (1) IL86065A (es)
SG (1) SG43288A1 (es)
WO (1) WO1988008034A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR8900881A (pt) * 1988-02-25 1989-10-17 Sandoz Ag Processos para a producao de uma proteina endotoxina toxica a insetos e composicao inseticida
US6946587B1 (en) 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
US6329574B1 (en) 1990-01-22 2001-12-11 Dekalb Genetics Corporation High lysine fertile transgenic corn plants
JP3209744B2 (ja) 1990-01-22 2001-09-17 デカルブ・ジェネティクス・コーポレーション 結実能力のある遺伝子変換コーン
TW261517B (es) * 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
WO1994028724A1 (en) * 1993-06-10 1994-12-22 Ecogen Inc. Bacillus thuringiensis strains capable of producing large amonts of insecticidal crystal proteins
US6326527B1 (en) 1993-08-25 2001-12-04 Dekalb Genetics Corporation Method for altering the nutritional content of plant seed
EP0973910A1 (en) 1997-03-13 2000-01-26 Mycogen Corporation Bacillus thuringiensis toxins

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4467036A (en) * 1981-11-12 1984-08-21 The Board Of Regents Of The University Of Washington Bacillus thuringiensis crystal protein in Escherichia coli
WO1982003872A1 (en) * 1981-04-27 1982-11-11 Washington Univ B.thuringiensis crystal protein in e.coli
US4695455A (en) * 1985-01-22 1987-09-22 Mycogen Corporation Cellular encapsulation of pesticides produced by expression of heterologous genes
US5080897A (en) * 1987-05-08 1992-01-14 Ecogen Inc. Novel bacillus thuringiensis strains, and related insecticidal compositions

Also Published As

Publication number Publication date
JPH0636743B2 (ja) 1994-05-18
GR1000679B (el) 1992-10-08
DK513689A (da) 1989-12-14
KR960006582B1 (ko) 1996-05-20
DE3855827T2 (de) 1997-11-27
IL86065A0 (en) 1988-09-30
GR880100247A (en) 1989-01-31
IL86065A (en) 1994-10-07
FI894856A0 (fi) 1989-10-13
KR890700677A (ko) 1989-04-26
DE3855827D1 (de) 1997-04-17
CA1340600C (en) 1999-06-22
AU1681788A (en) 1988-11-04
AU618532B2 (en) 1992-01-02
ATE150089T1 (de) 1997-03-15
JPH02500716A (ja) 1990-03-15
EP0367767A4 (en) 1990-05-14
FI894856A7 (fi) 1989-10-13
EP0367767B1 (en) 1997-03-12
WO1988008034A1 (en) 1988-10-20
DK513689D0 (da) 1989-10-16
SG43288A1 (en) 1997-10-17
EP0367767A1 (en) 1990-05-16

Similar Documents

Publication Publication Date Title
ATE174626T1 (de) Neuer mikroorganismus und insektizid
ATE178457T1 (de) Verwendung von streptomyces wyec 108 zur bekämpfung von pflanzenschädlingen
IL124020A (en) Plant optimized nucleotide sequence that encodes an insecticidal crystal protein
DK161785D0 (da) Fremgangsmaade til selektiv ukrudtsbekaempelse i nytteplantekulturer
NL350040I2 (nl) Samenstelling en werkwijze voor het bestrijden vanplantenziekten
DE69006015D1 (de) Pflanzen, transformiert zur herstellung von insektiziden toxinen von bacillus thuringiensis.
MY129859A (en) Bacterial strains of the bacterium bacillus thuringiensis and uses therefor
ES2109182B1 (es) Formulacion liquida a base de cepas de los hongos filamentosos trichoderma harzianum y trichoderma viride.
ES2043610T3 (es) Procedimiento para la produccion de un polipeptido hibrido del tipo activador de plasminogeno.
IL86893A (en) Ab-006 antibiotics, their production and their use as fungicides and insecticides
ATE150089T1 (de) Bacillus thuringiensis p-2-toxingen, protein und damit in zusammenhang stehende insektizide zusammensetzungen
GB8800652D0 (en) Bacterial strain
ES2060233T3 (es) Streptomyces ncimb 40227 activo en la bioestimulacion de la produccion agricola.
MX9700042A (es) Aislados de bacillus thurigiensis activo contra cucarachas.
ES2044936T3 (es) Productos y procedimientos agricolas.
GB1323806A (en) Microbial pesticides and processes for producing them
ES2052716T3 (es) Bacillus thuringiensis seleccionado de acetato y metodo de utilizacion.
DK475979A (da) Antibiotikum fremgangsmaade til dets fremstilling og dets anvendelse som laegemiddel
IL90575A0 (en) Ab-011 antibiotics,their production and compositions containing them
ES8103163A1 (es) Un procedimiento para la obtencion de una preparacion biolo-gica para la estimulacion de la actividad vital
CU22795A1 (es) Estreptotricinas b y f, fungicidas naturales para el control de fitopatógenos en bananos y platanos

Legal Events

Date Code Title Description
PC1A Transfer granted