ES2033266T3 - Procedimiento y estuche de ensayo para la determinacion de sustancias activas libres en liquidos biologicos. - Google Patents

Procedimiento y estuche de ensayo para la determinacion de sustancias activas libres en liquidos biologicos.

Info

Publication number
ES2033266T3
ES2033266T3 ES198787111139T ES87111139T ES2033266T3 ES 2033266 T3 ES2033266 T3 ES 2033266T3 ES 198787111139 T ES198787111139 T ES 198787111139T ES 87111139 T ES87111139 T ES 87111139T ES 2033266 T3 ES2033266 T3 ES 2033266T3
Authority
ES
Spain
Prior art keywords
active agent
antibody
free
bound
determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198787111139T
Other languages
English (en)
Inventor
Guido Dr. Simons
Helmut Dr. Strecker
Peter Dr. Molz
Gerd Dr. Schnorr
Heinz Jurgen Dr. Skrzipczyk
Hans Dr. Wissmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Application granted granted Critical
Publication of ES2033266T3 publication Critical patent/ES2033266T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0004—Gaseous mixtures, e.g. polluted air
    • G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
    • G01N33/0027—General constructional details of gas analysers, e.g. portable test equipment concerning the detector
    • G01N33/0036—General constructional details of gas analysers, e.g. portable test equipment concerning the detector specially adapted to detect a particular component
    • G01N33/0055—Radionuclides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Combustion & Propulsion (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PROCEDIMIENTO PARA DETERMINACION DE CONCENTRACION DE COMPONENTES LIBRES DE UNA SUSTANCIA EXISTENTE EN UN LIQUIDO BIOLOGICO EN PRESENCIA DE AGLUTINANTES NATURALES, CON LO QUE EL COMPONENTE LIBRE Y EL AGLUTINADO DE LA SUSTANCIA ESTAN EN EQUILIBRIO UNO CON OTRO, MIENTRAS SE A) LLEVA UNA PRUEBA DEL LIQUIDO EN CONTACTO CON UN ANTICUERPO NO MARCADO; B) SE SEPARA LA PRUEBA DEL ANTICUERPO NO MARCADO; C) SE INCUBA EL ANTICUERPO NO MARCADO CON UNO MARCADO, CON SUSTANCIA (TRACER) REACTIVA EN CRUZ; D) SE PRESCINDE DEL COMPONENETE DEL TRACER QUE ESTA O NO AGLUTINADO EN EL ANTICUERPO Y SE CALCULA LA CONCENTRACION DEL COMPONENETE LIBRE DE LA SUSTANCIA, CON LO QUE LA CANTIDAD DEL ANTICUERPO NO MARCADO Y/O SU AFINIDAD A LA SUSTANCIA ES TAN PEQUEÑA QUE NO SE INFLUENCIA EL EQUILIBRIO ENTRE EL COMPONENTE LIBRE Y AGLUTINADO DE LA SUSTANCIA Y EL TRACER TIENE UNA AFINIDAD CONSIDERABLEMENTE MAYOR O CONSIDERABLEMENTE MENOR AL ANTICUERPO QUE LA PROPIA SUSTANCIA Y UN JUEGO DE PRUEBA APROPIADO PARA ESTE PROCEDIMIENTO.
ES198787111139T 1986-08-05 1987-08-01 Procedimiento y estuche de ensayo para la determinacion de sustancias activas libres en liquidos biologicos. Expired - Lifetime ES2033266T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19863626468 DE3626468A1 (de) 1986-08-05 1986-08-05 Verfahren und testkit zur bestimmung freier wirkstoffe in biologischen fluessigkeiten

Publications (1)

Publication Number Publication Date
ES2033266T3 true ES2033266T3 (es) 1993-03-16

Family

ID=6306719

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198787111139T Expired - Lifetime ES2033266T3 (es) 1986-08-05 1987-08-01 Procedimiento y estuche de ensayo para la determinacion de sustancias activas libres en liquidos biologicos.

Country Status (9)

Country Link
US (2) US5714336A (es)
EP (1) EP0257352B1 (es)
JP (1) JPH0743375B2 (es)
AT (1) ATE77149T1 (es)
CA (1) CA1304680C (es)
DE (2) DE3626468A1 (es)
DK (1) DK172582B1 (es)
ES (1) ES2033266T3 (es)
NO (1) NO169979C (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3624464A1 (de) * 1986-07-19 1988-01-28 Boehringer Mannheim Gmbh Verfahren zum nachweis eines analyten sowie hierfuer geeignetes mittel
JP2003514524A (ja) 1999-11-18 2003-04-22 コルバス・インターナショナル・インコーポレイテッド エントセリアーゼをコードしている核酸、エンドセリアーゼおよびその使用
US6638977B1 (en) * 1999-11-19 2003-10-28 Corvas International, Inc. Plasminogen activator inhibitor antagonists
WO2001036351A2 (en) 1999-11-19 2001-05-25 Corvas International, Inc. Plasminogen activator inhibitor antagonists related applications
US7700341B2 (en) 2000-02-03 2010-04-20 Dendreon Corporation Nucleic acid molecules encoding transmembrane serine proteases, the encoded proteins and methods based thereon
WO2002072786A2 (en) 2001-03-13 2002-09-19 Corvas International, Inc. Nucleic acid molecules encoding a transmembrane serine protease 7, the encoded polypeptides and methods based thereon
US7172892B2 (en) 2001-03-22 2007-02-06 Dendreon Corporation Nucleic acid molecules encoding serine protease CVSP14, the encoded polypeptides and methods based thereon
CA2442089A1 (en) 2001-03-27 2002-10-03 Dendreon San Diego Llc Nucleic acid molecules encoding a transmembran serine protease 9, the encoded polypeptides and methods based thereon
JP2005506832A (ja) 2001-05-14 2005-03-10 デンドレオン・サンディエゴ・リミテッド・ライアビリティ・カンパニー トランスメンブランセリンプロテアーゼ１０をコードする核酸分子、コードされるポリペプチドおよびそれに基づく方法
US7589029B2 (en) * 2002-05-02 2009-09-15 Micron Technology, Inc. Atomic layer deposition and conversion
US7160577B2 (en) * 2002-05-02 2007-01-09 Micron Technology, Inc. Methods for atomic-layer deposition of aluminum oxides in integrated circuits
US7084078B2 (en) * 2002-08-29 2006-08-01 Micron Technology, Inc. Atomic layer deposited lanthanide doped TiOx dielectric films
US7192892B2 (en) 2003-03-04 2007-03-20 Micron Technology, Inc. Atomic layer deposited dielectric layers
US7588988B2 (en) * 2004-08-31 2009-09-15 Micron Technology, Inc. Method of forming apparatus having oxide films formed using atomic layer deposition
US7662729B2 (en) * 2005-04-28 2010-02-16 Micron Technology, Inc. Atomic layer deposition of a ruthenium layer to a lanthanide oxide dielectric layer
US7572695B2 (en) * 2005-05-27 2009-08-11 Micron Technology, Inc. Hafnium titanium oxide films
US7927948B2 (en) 2005-07-20 2011-04-19 Micron Technology, Inc. Devices with nanocrystals and methods of formation
US7575978B2 (en) 2005-08-04 2009-08-18 Micron Technology, Inc. Method for making conductive nanoparticle charge storage element
US7410910B2 (en) 2005-08-31 2008-08-12 Micron Technology, Inc. Lanthanum aluminum oxynitride dielectric films
US7763511B2 (en) * 2006-12-29 2010-07-27 Intel Corporation Dielectric barrier for nanocrystals
US8367506B2 (en) 2007-06-04 2013-02-05 Micron Technology, Inc. High-k dielectrics with gold nano-particles
DE102023108022A1 (de) 2023-03-29 2024-10-02 Forschungszentrum Jülich GmbH Neuer L-Thyroxin-Radioiodmarker und Verfahren zu seiner Herstellung

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3928553A (en) * 1972-03-23 1975-12-23 Univ New York Radioimmunoassay method for triiodothyronine and thyroxine
US4292296A (en) * 1978-09-12 1981-09-29 Baxter Travenol Laboratories, Inc. Diagnostic method
US4366143A (en) * 1979-09-24 1982-12-28 Amersham International Public Limited Company Assay for the free portion of substances in biological fluids
WO1981001414A1 (en) * 1979-11-19 1981-05-28 Charles Hospital Dev An improved method of non homogenous enzyme immunoassay
US4391795A (en) * 1980-03-21 1983-07-05 Becton Dickinson And Company Assay for free thyroid hormone
US4426453A (en) * 1980-09-18 1984-01-17 Amersham International Limited Derivatives of iodothyronine compounds and their use in an assay for the free iodothyronine compounds
DE3122019C2 (de) * 1981-06-03 1983-11-10 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und Vorrichtung zur Durchführung eines automatisierten kontinuierlichen Enzymimmunotestes
US5304498A (en) * 1982-03-19 1994-04-19 Ekins Roger Philip Method and composition for free ligand assay
DE3380125D1 (en) * 1982-03-22 1989-08-03 Amersham Int Plc Assay for the free portion of substances in biological fluids
DE3380777D1 (en) * 1982-08-27 1989-11-30 Roger Philip Ekins Measurement of analyte concentration
ZA837119B (en) * 1982-10-07 1984-12-24 Amersham Int Plc Assay for the free portion of substances in biological fluids
GB8317124D0 (en) * 1983-06-23 1983-07-27 Ekins R P Free ligand assay
GB8404843D0 (en) * 1984-02-24 1984-03-28 Amersham Int Plc Free analyte assay
US4711855A (en) * 1985-02-05 1987-12-08 Ciba Corning Diagnostics Corp. Derivatives of 3,5,3-triiodothyronine
DE3600365A1 (de) * 1986-01-09 1987-07-16 Hoechst Ag Neue thyroninderivate
GB8800419D0 (en) * 1988-01-08 1988-02-10 Amersham Int Plc Method for measuring free fraction of ligands in biological fluids

Also Published As

Publication number Publication date
DE3779704D1 (de) 1992-07-16
NO169979C (no) 1992-08-26
EP0257352B1 (de) 1992-06-10
NO169979B (no) 1992-05-18
EP0257352A1 (de) 1988-03-02
NO873258L (no) 1988-02-08
ATE77149T1 (de) 1992-06-15
NO873258D0 (no) 1987-08-04
CA1304680C (en) 1992-07-07
JPH0743375B2 (ja) 1995-05-15
DK406087A (da) 1988-02-06
US6103486A (en) 2000-08-15
US5714336A (en) 1998-02-03
DK172582B1 (da) 1999-02-08
JPS6344168A (ja) 1988-02-25
DE3626468A1 (de) 1988-02-11
DK406087D0 (da) 1987-08-04

Similar Documents

Publication Publication Date Title
ES2033266T3 (es) Procedimiento y estuche de ensayo para la determinacion de sustancias activas libres en liquidos biologicos.
FI903515A0 (fi) Testimenetelmä ja reagenssikitti sitä varten
LV10538A (lv) Imunologiski saistamas vielas noteiksanas panemiens biologiska skidruma un diagnostikas lidzeklis
JPS5530692A (en) Method of immunological measuring
KR870004305A (ko) 고체-상 분석장치 및 분석방법
SE8600454L (sv) Testanordning for att bestemma forekomsten av organiska material samt forfarande for anordningens utnyttjande
EP0290921A3 (en) Test-piece for the analysis of a liquid sample and method for its preparation
ES2101802T3 (es) Ensayo para determinar los niveles de igf-i, igf-ii y gh libres en liquidos corporales.
ATE107418T1 (de) Nachweis von analyt in partikel enthaltenden proben.
KR880002015A (ko) 검체의 검출방법 및 시약
Glad et al. Immunocapillarymigration—a new method for immunochemical quantitation
DK516387A (da) Fremgangsmaade til immunometrisk bestemmelse
DE69223651D1 (de) Vorrichtungen zur ausführung von ioneneinfangassays
DE3584023D1 (de) Verfahren zur durchfuehrung von immunfluoreszenztesten.
DK219686D0 (da) Fremgangsmaade til bestemmelse af koncentrationen af en fri ligand i en biologisk vaeske
JPS5748658A (en) Immunologic measuring method and reagent for measurement
ES2042661T3 (es) Procedimiento de determinacion inmunologico para la determinacion de sustancias libres que poseen propiedades hapteno.
DE3781323D1 (de) Verfahren und testsatz zur diagnose von pflanzenkrankheiten.
DE69014087D1 (de) Verfahren zur quantitativen und qualitativen Bestimmung von Antikörpern gegen bakterielle Antigene mittels photometrischer Messung der Agglutination.
JI FEASIBILITY OF SCREENING FOR ANTIBODY TO HIV BY POOLING THE SERUM SPECIMENS
GB2011070A (en) Assay of vitamin B12
DE3786351D1 (de) Festphasenimmunoassayverfahren.
KR830004827A (ko) 혈장 내 피브리노오겐의 양을 신속하게 측정하는 방법

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 257352

Country of ref document: ES