ES2034154T3 - Microscopio de exploracion laser concentrico o de foco central. - Google Patents

Microscopio de exploracion laser concentrico o de foco central.

Info

Publication number
ES2034154T3
ES2034154T3 ES198888200452T ES88200452T ES2034154T3 ES 2034154 T3 ES2034154 T3 ES 2034154T3 ES 198888200452 T ES198888200452 T ES 198888200452T ES 88200452 T ES88200452 T ES 88200452T ES 2034154 T3 ES2034154 T3 ES 2034154T3
Authority
ES
Spain
Prior art keywords
scanning
point
microscope
frame
focused
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198888200452T
Other languages
English (en)
Inventor
Pieter Marcus Houpt
Arie Draaijer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Original Assignee
Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO filed Critical Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Application granted granted Critical
Publication of ES2034154T3 publication Critical patent/ES2034154T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0068—Optical details of the image generation arrangements using polarisation
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G02B21/0048—Scanning details, e.g. scanning stages scanning mirrors, e.g. rotating or galvanomirrors, MEMS mirrors
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0072—Optical details of the image generation details concerning resolution or correction, including general design of CSOM objectives
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G02B21/0084—Details of detection or image processing, including general computer control time-scale detection, e.g. strobed, ultra-fast, heterodyne detection

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Engineering & Computer Science (AREA)
  • Microscoopes, Condenser (AREA)
  • Mechanical Optical Scanning Systems (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

SE PRESENTA UN MICROSCOPIO DE EXPLORACION POR LASER QUE COMPRENDE UN LASER (1) COMO FUENTE DE LUZ PUNTUAL, UN SISTEMA DE DEFLECCION PARA LA LINEA Y UN SISTEMA DE LENTES, AL MENOS UN OBJETIVO (9) CERCANO AL OBJETO, UN PORTAOBJETOS, UN FILTRO ESPACIAL (14) Y UN DETECTOR (15), Y UN CONTROL ELECTRONICO Y UN SISTEMA DE PROCESAMIENTO DE IMAGEN. EL OBJETO ES EXPLORADO PUNTO POR PUNTO POR EL HAZ DE LUZ Y SE TOMAN MEDIDAS CON EL DETECTOR SOLAMENTE DONDE LA FUENTE DE LUZ PUNTUAL SE ENFOCA DE TAL FORMA QUE LA LUZ FUERA DE FOCO NO SE DETECTA. COMO RESULTADO LA RESOLUCION Y EL CONTRASTE EN TRES DIMENSIONES, EN PARTICULAR, AXIALMENTE AL PLANO DE LA IMAGEN PUEDE MEJORARSE CONSIDERABLEMENTE, Y LA INFORMACION EN TRES DIMENSIONES PUEDE OBTENERSE DEL OBJETO. PARA UNA EXPLORACION DE LINEA MAS RAPIDA, EL SISTEMA DE DEFLECCION DEL PRESENTE INVENTO COMPRENDE UN DEFLECTOR ACUSTOOPTICO (5) Y COMPRENDE PARA LA EXPLORACION DE MANERA MAS LENTA, OTRO DEFLECTOR (7) CONSTRUIDO EN UNA FORMA TAL QUE AL MENOS EL MOVIMIENTO DE EXPLORACION DEL HAZ DE LUZ DE RETORNO SE ELIMINA COMPLETAMENTE, COMO RESULTADO DE LO CUAL LA LUZ DE RETORNO SE ENFOCA SOBRE EL FILTRO ESPACIAL.
ES198888200452T 1987-03-13 1988-03-09 Microscopio de exploracion laser concentrico o de foco central. Expired - Lifetime ES2034154T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL8700612A NL8700612A (nl) 1987-03-13 1987-03-13 Confocale laserscanning microscoop.

Publications (1)

Publication Number Publication Date
ES2034154T3 true ES2034154T3 (es) 1993-04-01

Family

ID=19849704

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198888200452T Expired - Lifetime ES2034154T3 (es) 1987-03-13 1988-03-09 Microscopio de exploracion laser concentrico o de foco central.

Country Status (7)

Country Link
US (1) US4863226A (es)
EP (1) EP0284136B1 (es)
JP (2) JPS63298211A (es)
AT (1) ATE79476T1 (es)
DE (1) DE3873570T2 (es)
ES (1) ES2034154T3 (es)
NL (1) NL8700612A (es)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200838A (en) * 1988-05-27 1993-04-06 The University Of Connecticut Lateral effect imaging system
US5225924A (en) * 1989-04-07 1993-07-06 Dainippon Screen Mfg. Co., Ltd. Optical beam scanning system
JP2525894B2 (ja) * 1989-04-07 1996-08-21 浜松ホトニクス株式会社 半導体試料の螢光特性検査装置
JP2525893B2 (ja) * 1989-04-07 1996-08-21 浜松ホトニクス株式会社 螢光特性検査装置
US5002348A (en) * 1989-05-24 1991-03-26 E. I. Du Pont De Nemours And Company Scanning beam optical signal processor
EP0418928B1 (en) * 1989-09-22 1996-05-01 Fuji Photo Film Co., Ltd. Scanning microscope and scanning mechanism for the same
US5034613A (en) * 1989-11-14 1991-07-23 Cornell Research Foundation, Inc. Two-photon laser microscopy
US5386112A (en) * 1990-06-29 1995-01-31 Dixon; Arthur E. Apparatus and method for transmitted-light and reflected-light imaging
JP3280969B2 (ja) * 1991-04-10 2002-05-13 メイヨ ファンデーション フォー メディカル エデュケーション アンド リサーチ 可視光および紫外光の共焦点結像装置
US5260578A (en) * 1991-04-10 1993-11-09 Mayo Foundation For Medical Education And Research Confocal imaging system for visible and ultraviolet light
US5306902A (en) * 1992-09-01 1994-04-26 International Business Machines Corporation Confocal method and apparatus for focusing in projection lithography
JP3343276B2 (ja) * 1993-04-15 2002-11-11 興和株式会社 レーザー走査型光学顕微鏡
US5923430A (en) * 1993-06-17 1999-07-13 Ultrapointe Corporation Method for characterizing defects on semiconductor wafers
US5479252A (en) * 1993-06-17 1995-12-26 Ultrapointe Corporation Laser imaging system for inspection and analysis of sub-micron particles
USH1530H (en) * 1993-06-17 1996-05-07 Ultrapointe Corporation Surface extraction from a three-dimensional data set
KR950009292A (ko) * 1993-09-29 1995-04-21 유모토 다이죠 공초점형 광학현미경 및 이 현미경을 사용한 측정장치
US5923466A (en) * 1993-10-20 1999-07-13 Biophysica Technologies, Inc. Light modulated confocal optical instruments and method
US5587832A (en) * 1993-10-20 1996-12-24 Biophysica Technologies, Inc. Spatially light modulated confocal microscope and method
GB9408688D0 (en) * 1994-04-30 1994-06-22 Medical Res Council Scanning confocal optical microscope
US5561611A (en) * 1994-10-04 1996-10-01 Noran Instruments, Inc. Method and apparatus for signal restoration without knowledge of the impulse response function of the signal acquisition system
DE19510102C1 (de) * 1995-03-20 1996-10-02 Rainer Dr Uhl Konfokales Fluoreszenzmikroskop
WO1997011355A1 (en) * 1995-09-19 1997-03-27 Cornell Research Foundation, Inc. Multi-photon laser microscopy
US6148114A (en) * 1996-11-27 2000-11-14 Ultrapointe Corporation Ring dilation and erosion techniques for digital image processing
DE19758744C2 (de) * 1997-01-27 2003-08-07 Zeiss Carl Jena Gmbh Laser-Scanning-Mikroskop
US6771417B1 (en) * 1997-08-01 2004-08-03 Carl Zeiss Jena Gmbh Applications of adaptive optics in microscopy
DE19733193B4 (de) * 1997-08-01 2005-09-08 Carl Zeiss Jena Gmbh Mikroskop mit adaptiver Optik
US6366357B1 (en) 1998-03-05 2002-04-02 General Scanning, Inc. Method and system for high speed measuring of microscopic targets
US6098031A (en) * 1998-03-05 2000-08-01 Gsi Lumonics, Inc. Versatile method and system for high speed, 3D imaging of microscopic targets
US5936728A (en) * 1998-04-14 1999-08-10 Noran Instruments, Inc. Flash photolysis method and apparatus
US6720547B1 (en) * 1999-03-18 2004-04-13 Lucid, Inc. System and method for enhancing confocal reflectance images of tissue specimens
WO2000055669A1 (en) * 1999-03-18 2000-09-21 Lucid, Inc. System and method for enhancing confocal reflectance images of tissue specimens
US6548796B1 (en) * 1999-06-23 2003-04-15 Regents Of The University Of Minnesota Confocal macroscope
US6449039B1 (en) 1999-07-28 2002-09-10 Thermo Noran Inc. Laser scanning fluorescence microscopy with compensation for spatial dispersion of fast laser pulses
DE10050529B4 (de) * 2000-10-11 2016-06-09 Leica Microsystems Cms Gmbh Verfahren zur Strahlsteuerung in einem Scanmikroskop, Anordnung zur Strahlsteuerung in einem Scanmikroskop und Scanmikroskop
US7139122B1 (en) * 2000-10-17 2006-11-21 Lucid, Inc. System and method for enhancing confocal reflectance images of tissue specimens
AU2002245119A1 (en) 2000-12-15 2002-07-30 Sloan-Kettering Institute For Cancer Research Beam-steering of multi-chromatic light using acousto-optical deflectors and dispersion-compensatory optics
DE10107210C1 (de) * 2001-02-16 2002-10-10 Evotec Ag Mikroskop
US6750974B2 (en) 2002-04-02 2004-06-15 Gsi Lumonics Corporation Method and system for 3D imaging of target regions
WO2004038461A2 (en) * 2002-10-22 2004-05-06 William Marsh Rice University Random access high-speed confocal microscope
GB0308072D0 (en) * 2003-04-08 2003-05-14 Visitech Internat Ltd Fast multi-line laser confocal scanning microscope
JP4987233B2 (ja) * 2005-01-06 2012-07-25 オリンパス株式会社 レーザ走査型顕微鏡
US9055867B2 (en) * 2005-05-12 2015-06-16 Caliber Imaging & Diagnostics, Inc. Confocal scanning microscope having optical and scanning systems which provide a handheld imaging head
DE102007021378A1 (de) * 2007-05-04 2008-11-06 Ape Angewandte Physik Und Elektronik Gmbh Verfahren und optische Anordnung zum Erzeugen eines nicht-linearen optischen Signals an einem durch ein Anregungsfeld angeregten Material sowie Verwendung des Verfahrens und der optischen Anordnung
KR101119381B1 (ko) * 2009-06-18 2012-03-07 한국과학기술원 세포 특성 측정 장치 및 시스템과 이를 이용한 세포 특성 측정 방법
CN101904735B (zh) * 2010-07-20 2013-05-08 苏州微清医疗器械有限公司 基于快速倾斜镜的宽视场共焦扫描显微镜
CN102885612A (zh) * 2012-02-14 2013-01-23 苏州微清医疗器械有限公司 一种用于临床诊断的眼底成像设备
CN105486638B (zh) * 2015-11-30 2019-02-05 哈尔滨工业大学 一种超分辨阵列扫描结构光照明成像装置及其成像方法
CN106442335B (zh) * 2016-12-16 2024-04-09 中国科学院工程热物理研究所 一种显微可视化抽运探测热反射系统
CN108519329B (zh) * 2018-03-26 2021-01-15 华中科技大学 一种多路扫描与探测的线共聚焦成像装置
CN112261253B (zh) * 2020-10-15 2022-05-03 浙江大华技术股份有限公司 共焦补偿滤片的控制方法、装置、存储介质以及电子装置
WO2023193480A1 (zh) * 2022-04-08 2023-10-12 南京博视医疗科技有限公司 一种视网膜成像装置及其成像方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5118136B1 (es) * 1970-11-25 1976-06-08
US4044363A (en) * 1974-12-23 1977-08-23 Dymo Industries, Inc. Laser photocomposition system and method
US4634880A (en) * 1982-04-19 1987-01-06 Siscan Systems, Inc. Confocal optical imaging system with improved signal-to-noise ratio
GB2152697B (en) * 1983-10-25 1987-03-25 Atomic Energy Authority Uk Improvements in or relating to scanning optical microscopes
DE3427611A1 (de) * 1984-07-26 1988-06-09 Bille Josef Laserstrahl-lithograph
US4640626A (en) * 1984-09-13 1987-02-03 Siemens Aktiengesellschaft Method and apparatus for localizing weak points within an electrical circuit
JPS61118710A (ja) * 1984-11-15 1986-06-06 Nippon Jido Seigyo Kk 走査型顕微鏡撮像装置
JPS61121022A (ja) * 1984-11-19 1986-06-09 Nippon Jido Seigyo Kk 走査型顕微鏡撮像装置
JPS61140914A (ja) * 1984-12-14 1986-06-28 Hitachi Ltd 共焦点顕微鏡
JP2524574B2 (ja) * 1985-03-27 1996-08-14 オリンパス光学工業株式会社 走査型光学顕微鏡

Also Published As

Publication number Publication date
JP2607804Y2 (ja) 2003-03-31
DE3873570D1 (de) 1992-09-17
DE3873570T2 (de) 1994-01-05
JPS63298211A (ja) 1988-12-06
EP0284136B1 (en) 1992-08-12
NL8700612A (nl) 1988-10-03
ATE79476T1 (de) 1992-08-15
JPH11135U (ja) 1999-11-09
US4863226A (en) 1989-09-05
EP0284136A1 (en) 1988-09-28

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