ES2045081T3 - Amplificacion estable de genes en adn cromosonal procariotica. - Google Patents

Amplificacion estable de genes en adn cromosonal procariotica.

Info

Publication number
ES2045081T3
ES2045081T3 ES88200376T ES88200376T ES2045081T3 ES 2045081 T3 ES2045081 T3 ES 2045081T3 ES 88200376 T ES88200376 T ES 88200376T ES 88200376 T ES88200376 T ES 88200376T ES 2045081 T3 ES2045081 T3 ES 2045081T3
Authority
ES
Spain
Prior art keywords
strains
polypeptide
transformed
hosting
bacillus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88200376T
Other languages
English (en)
Inventor
Eekelen Christiaan Albertu Van
Der Laan Johannes Cornelis Van
Leonardus Johannes S Mulleners
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gist Brocades NV
Original Assignee
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8197582&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2045081(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gist Brocades NV filed Critical Gist Brocades NV
Application granted granted Critical
Publication of ES2045081T3 publication Critical patent/ES2045081T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LOS HOSPEDADORES PROCARITAS TRANSFORMADOS SE PROPORCIONAN INCLUYENDO DOS O MAS COPIAS DE LA SECUENCIA DE ADN ESTABLE MANTENIDA EN SU CROMOSOMA, DICHA SECUENCIA COMPRENDE UN GEN QUE CODIFICA UN POLIPEPTIDO DE INTERES, EN DONDE DICHAS COPIAS SE SEPARAN POR SECUENCIAS DE ADN CROMOSOMAL ENDOGENO. LOS METODOS TAMBIEN PROPORCIONAN LA PRODUCCION DE DICHAS CEPAS HOSPEDADORAS TRANSFORMADAS. DICHAS CEPAS HOSPEDADORAS TRANSFORMADAS SON CAPACES DE INCREMENTAR LA PRODUCCION DEL POLIPEPTIDO DE INTERES EN COMPARACION CON LAS CEPAS HOSPEDADORAS QUE YA PRODUCEN DICHO POLIPEPTIDO. LAS CEPAS HOSPEDADORAS PREFERIDAS SON BACILLUS ESPECIE NUEVA PB92 QUE PRODUCE UN ENZIMA PROTEOLITICO MUY ALCALINO Y BACILLUS LICHENIFORMS T5 QUE PRODUCE UNA ALFA-AMILASA TERMOESTABLE Y MUTANTES Y VARIANTES DE DICHAS CEPAS. EL GEN CODIFICADOR DE POLIPEPTIDOS PREFERIDO ES EL GEN CODIFICADOR DE LA PROTEASA ORIGINADA POR BACILLUS PB92 Y GEN CODIFICADOR DE LA ALFA-AMILASA ORIGINADA POR BACILLUS LICHENIFORMIS CEPA T5.
ES88200376T 1987-02-27 1988-02-29 Amplificacion estable de genes en adn cromosonal procariotica. Expired - Lifetime ES2045081T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP87200356 1987-02-27

Publications (1)

Publication Number Publication Date
ES2045081T3 true ES2045081T3 (es) 1994-01-16

Family

ID=8197582

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88200376T Expired - Lifetime ES2045081T3 (es) 1987-02-27 1988-02-29 Amplificacion estable de genes en adn cromosonal procariotica.

Country Status (20)

Country Link
US (2) US5733723A (es)
EP (1) EP0284126B1 (es)
JP (1) JP2637532B2 (es)
KR (1) KR970000188B1 (es)
CN (1) CN1049247C (es)
AU (1) AU620326B2 (es)
BG (1) BG60920B2 (es)
BR (1) BR8805646A (es)
CA (1) CA1327175C (es)
DE (1) DE3883041T2 (es)
ES (1) ES2045081T3 (es)
FI (1) FI104326B1 (es)
HU (1) HUT50877A (es)
IE (1) IE61743B1 (es)
LT (1) LT4001B (es)
LV (1) LV10791B (es)
NO (1) NO300382B1 (es)
PT (1) PT86863B (es)
RU (1) RU2091487C1 (es)
WO (1) WO1988006623A1 (es)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2135386T3 (es) * 1987-02-27 1999-11-01 Genencor Int Transformacion de cepas de bacillus alcalofilas.
AU620326B2 (en) * 1987-02-27 1992-02-20 Dsm Ip Assets B.V. Stable gene amplification in chromosomal dna of prokaryotic microorganisms
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
ES2095233T3 (es) * 1989-08-11 1997-02-16 Gist Brocades Nv Produccion eficaz de proteasa mutante.
US5695976A (en) * 1989-12-18 1997-12-09 Novo Nordisk A/S Stable integration of DNA in bacterial genomes
JP3046344B2 (ja) * 1990-10-24 2000-05-29 塩野義製薬株式会社 新規プロテアーゼ
JPH07503363A (ja) * 1991-11-14 1995-04-13 ノボザイムス アクティーゼルスカブ バシラス・リヘニフォルミス中で遺伝子を発現させる方法
DE4216246A1 (de) * 1992-05-16 1993-11-18 Solvay Enzymes Gmbh & Co Kg Einfaches Verfahren zur stabilen chromosomalen Genamplifikation
US5395763A (en) * 1992-06-24 1995-03-07 Monsanto Company Chromosomal expression vector
DK153992D0 (da) * 1992-12-22 1992-12-22 Novo Nordisk As Metode
US5861273A (en) * 1993-12-21 1999-01-19 Celtrix Phamraceuticals, Inc. Chromosomal expression of heterologous genes in bacterial cells
US6723550B1 (en) 1997-07-15 2004-04-20 Genencor International, Inc. Proteases from gram-positive organisms
GB9724627D0 (en) 1997-11-20 1998-01-21 Genencor Int Bv Gram positive microorganism formate pathway
US6599731B1 (en) 1997-12-30 2003-07-29 Genencor International, Inc. Proteases from gram positive organisms
US6528255B1 (en) 1997-12-30 2003-03-04 Genencor International, Inc. Proteases from gram positive organisms
GB9727470D0 (en) 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms
US6465186B1 (en) 1997-12-30 2002-10-15 Genecor International, Inc. Proteases from gram positive organisms
US6100063A (en) * 1998-02-12 2000-08-08 Novo Nordisk A/S Procaryotic cell comprising at least two copies of a gene
ATE265523T1 (de) * 1998-02-12 2004-05-15 Novozymes As Prokaryontische zelle, die zwei kopien eines genes enthält, die in zwei verschiedene richtungen transkribiert werden.
RU2144565C1 (ru) * 1998-11-12 2000-01-20 Государственный научный центр вирусологии и биотехнологии "Вектор" Рекомбинантная плазмидная днк pq_f35, кодирующая гибридный полипептид f 35, обладающий антигенными и иммуногенными свойствами белка vp 35 вируса марбург, способ ее получения и штамм бактерий escherichia coli - сверхпродуцент рекомбинантного полипептида f 35
US6156514A (en) * 1998-12-03 2000-12-05 Sunol Molecular Corporation Methods for making recombinant cells
US6544792B1 (en) 1999-12-21 2003-04-08 Genencor International, Inc. Production of secreted polypeptides
EP1297170B1 (en) * 2000-06-23 2016-03-02 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
WO2005052146A2 (en) 2003-11-19 2005-06-09 Genencor International, Inc. Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
US20050227356A1 (en) * 2004-04-07 2005-10-13 Lessard Philip A Novel compositions and methods for genetic manipulation of Rhodococcus bacteria
US7842479B2 (en) * 2004-05-21 2010-11-30 The Board Of Regents Of The University Of Nebraska Methods for altering acetic acid production and enhancing cell death in bacteria
EP1766002B1 (en) * 2004-06-21 2015-11-18 Novozymes A/S Stably maintained multiple copies of at least two orf in the same orientation
DK1805296T3 (da) 2004-10-22 2010-01-18 Novozymes As Stabil genomisk integrering af multipie polynukleotidkopier
DE102007021001A1 (de) 2007-05-04 2008-11-06 Ab Enzymes Gmbh Expressionssystem zur antibiotikafreien Produktion von Polypeptiden
CN103290048B (zh) * 2013-06-27 2015-07-15 天津科技大学 一种适用于芽孢杆菌属同源重组的质粒及工程菌
ES2855992T3 (es) 2015-12-11 2021-09-27 Wacker Chemie Ag Cepa de microorganismos y procedimiento para la producción fermentativa exenta de antibióticos de sustancias y proteínas de bajo peso molecular
CN114032189B (zh) * 2021-05-10 2024-02-06 盐城师范学院 一种降解木质素的融合子菌株r3及其应用
GB202304540D0 (en) 2023-03-28 2023-05-10 Univ Tartu Method of biosynthesis

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NO159863C (no) * 1980-01-07 1989-02-15 Univ Rochester Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme.
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
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US4617266A (en) * 1983-04-28 1986-10-14 Genex Corporation Production of Protein A
JPS59205983A (ja) * 1983-04-28 1984-11-21 ジエネツクス・コ−ポレイシヨン 異種遺伝子を原核微生物で発現させる方法
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
IE58014B1 (en) * 1983-07-06 1993-06-16 Gist Brocades Nv Molecular cloning and expression in industrial microorganism species
FR2563533B1 (fr) * 1984-04-27 1986-08-22 Centre Nat Rech Scient Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
FR2582316B1 (fr) * 1985-05-22 1988-12-02 Centre Nat Rech Scient Vecteurs d'expression de l'a-amylase dans bacillus subtilis, souches obtenues et procede de preparation d'a-amylase
WO1987004461A1 (en) * 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF
AU620326B2 (en) * 1987-02-27 1992-02-20 Dsm Ip Assets B.V. Stable gene amplification in chromosomal dna of prokaryotic microorganisms

Also Published As

Publication number Publication date
KR890700664A (ko) 1989-04-26
LV10791A (lv) 1995-08-20
PT86863A (pt) 1988-03-01
AU1398188A (en) 1988-09-26
FI884903A0 (fi) 1988-10-24
NO300382B1 (no) 1997-05-20
BR8805646A (pt) 1989-10-17
JP2637532B2 (ja) 1997-08-06
HUT50877A (en) 1990-03-28
AU620326B2 (en) 1992-02-20
EP0284126B1 (en) 1993-08-11
CA1327175C (en) 1994-02-22
DE3883041T2 (de) 1994-01-20
LTIP1826A (en) 1995-08-25
LV10791B (en) 1995-12-20
IE61743B1 (en) 1994-11-30
NO884422L (no) 1988-10-05
RU2091487C1 (ru) 1997-09-27
FI104326B (fi) 1999-12-31
EP0284126A1 (en) 1988-09-28
KR970000188B1 (en) 1997-01-06
WO1988006623A1 (en) 1988-09-07
JPH01502398A (ja) 1989-08-24
CN1030787A (zh) 1989-02-01
CN1049247C (zh) 2000-02-09
PT86863B (pt) 1992-05-29
LT4001B (en) 1996-06-25
US5733723A (en) 1998-03-31
NO884422D0 (no) 1988-10-05
BG60920B2 (bg) 1996-06-28
US6124097A (en) 2000-09-26
IE880558L (en) 1988-08-27
FI104326B1 (fi) 1999-12-31
FI884903A7 (fi) 1988-10-24
DE3883041D1 (de) 1993-09-16

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