ES2064077T3 - Integracion estable de adn en genomas bacterianos. - Google Patents

Integracion estable de adn en genomas bacterianos.

Info

Publication number
ES2064077T3
ES2064077T3 ES91901587T ES91901587T ES2064077T3 ES 2064077 T3 ES2064077 T3 ES 2064077T3 ES 91901587 T ES91901587 T ES 91901587T ES 91901587 T ES91901587 T ES 91901587T ES 2064077 T3 ES2064077 T3 ES 2064077T3
Authority
ES
Spain
Prior art keywords
dna
stable integration
bacterial genomes
genome
reproduction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91901587T
Other languages
English (en)
Other versions
ES2064077T5 (es
Inventor
Steen Troels Jorgensen
Per Lina Jorgensen
Borge Krag Diderichsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8148687&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2064077(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of ES2064077T3 publication Critical patent/ES2064077T3/es
Publication of ES2064077T5 publication Critical patent/ES2064077T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

INTEGRACION ESTABLE DE ADN EN GENOMAS BACTERIANOS UNA CELULA BACTERIANA EN QUE EN SU GENOMA LLEVA UNA FORMACION DE ADN SIN REPETICION INTEGRADA QUE CONSTA DE (1) UNA SECUENCIA DE ADN DE INTERES, (2) UNA SECUENCIA DE ADN HOMOLOGA A LA REGION DEL GENOMA DE LA CELULA Y (3) UN ORIGEN DE REPRODUCCION, FALTANDOLE A DICHA FORMACION DE ADN UN GEN FUNCIONAL QUE CODIFIQUE EL FACTOR REQUERIDO PARA INICIAR LA REPETICION DE DICHO ORIGEN DE REPRODUCCION.
ES91901587T 1989-12-18 1990-12-18 Integracion estable de adn en genomas bacterianos. Expired - Lifetime ES2064077T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK639689A DK639689D0 (da) 1989-12-18 1989-12-18 Indfoering af dna i celler
DK639689 1989-12-18

Publications (2)

Publication Number Publication Date
ES2064077T3 true ES2064077T3 (es) 1995-01-16
ES2064077T5 ES2064077T5 (es) 2004-09-01

Family

ID=8148687

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91901587T Expired - Lifetime ES2064077T5 (es) 1989-12-18 1990-12-18 Integracion estable de adn en genomas bacterianos.

Country Status (11)

Country Link
EP (1) EP0506780B2 (es)
JP (1) JP3162707B2 (es)
AT (1) ATE113077T1 (es)
AU (1) AU643741B2 (es)
CA (1) CA2070676A1 (es)
DE (1) DE69013520T3 (es)
DK (2) DK639689D0 (es)
ES (1) ES2064077T5 (es)
FI (1) FI112502B (es)
NO (1) NO922381L (es)
WO (1) WO1991009129A1 (es)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2665711B1 (fr) * 1990-08-08 1993-08-13 Centre Nat Rech Scient Integron de corynebacterie, procede de transformation d'une corynebacterie par ledit integron et corynebacterie obtenue.
IL110299A0 (en) 1993-07-15 1994-10-21 Novo Nordisk Entotech Inc Formation of and methods for the production of large bacillus thuringiensis crystals with increased pesticidal activity
WO1996029418A1 (en) * 1995-03-22 1996-09-26 Novo Nordisk A/S Introduction of dna into bacillus strains by conjugation
FR2736066B1 (fr) * 1995-06-30 1998-11-20 Ajinomoto Kk Procede d'amplification d'un gene par transposon artificiel, bacterie coryneforme obtenue par ce procede et procede de production d'un acide amine a l'aide de cette bacterie
EP0878552A1 (en) 1997-05-13 1998-11-18 Erasmus Universiteit Rotterdam Molecular detection of chromosome aberrations
CN100392077C (zh) 1997-12-22 2008-06-04 诺维信公司 糖氧化酶及其在焙烤中的用途
JP2002513587A (ja) 1998-05-04 2002-05-14 ダコ エー エス 染色体異常を検出するための方法およびプローブ
BRPI9911086B1 (pt) 1998-06-10 2016-08-02 Novozymes As composição de limpeza, processo para tratar tecidos a máquina, e uso de uma mananase
JP3958884B2 (ja) 1998-09-11 2007-08-15 株式会社林原生物化学研究所 非還元性糖質生成酵素及びトレハロース遊離酵素ならびに該酵素を用いる糖質の製造方法
CN1197965C (zh) 1998-10-26 2005-04-20 诺维信公司 在丝状真菌细胞内构建和筛选目的dna文库
AU2001265835A1 (en) 2000-06-23 2002-01-08 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
ES2521615T3 (es) 2001-06-06 2014-11-13 Novozymes A/S Endo-beta-1,4-glucanasa
EP1696035A3 (en) 2002-04-10 2008-04-02 Novozymes A/S Improved bacillus host cell
EP1995319B1 (en) 2002-04-10 2011-08-31 Novozymes A/S Bacillus licheniformis mutant host cell
AU2003223928A1 (en) * 2002-05-07 2003-11-11 Novozymes A/S Homologous recombination into bacterium for the generation of polynucleotide libraries
MXPA05006071A (es) 2002-12-11 2005-09-30 Novozymes As Composicion detergente.
WO2005056799A1 (en) 2003-12-10 2005-06-23 Novozymes A/S A cell with improved secretion mediated by mrga protein or homologue
WO2005123915A1 (en) 2004-06-21 2005-12-29 Novozymes A/S Stably maintained multiple copies of at least two orf in the same orientation
US7820408B2 (en) 2006-11-29 2010-10-26 Novozymes, Inc. Methods of improving the introduction of DNA into bacterial cells
WO2008140615A2 (en) 2006-12-21 2008-11-20 Novozymes, Inc. Modified messenger rna stabilizing sequences for expressing genes in bacterial cells
CN102762723A (zh) 2009-12-21 2012-10-31 诺维信股份有限公司 用于在巯基-二硫键氧还酶缺陷的细菌突变体细胞中产生异源多肽的方法
WO2018077796A1 (en) 2016-10-25 2018-05-03 Novozymes A/S Flp-mediated genomic integrationin bacillus licheniformis
EP3655528A1 (en) 2017-07-21 2020-05-27 Basf Se Method of transforming bacterial cells
DK3655422T5 (da) 2017-07-21 2024-08-26 Basf Se Promotor til heterolog ekspression
EP3707252A1 (en) 2017-11-10 2020-09-16 Novozymes A/S Temperature-sensitive cas9 protein
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
US12492391B2 (en) 2019-01-30 2025-12-09 Novozymes A/S Cognate foldase co-expression
WO2020169564A1 (en) 2019-02-20 2020-08-27 Basf Se Industrial fermentation process for bacillus using defined medium and trace element feed
CN114096676A (zh) 2019-02-20 2022-02-25 巴斯夫欧洲公司 用确定成分培养基和镁补料的芽孢杆菌工业发酵工艺
EP3935160A1 (en) 2019-03-08 2022-01-12 Novozymes A/S Means and methods for improving protease expression
CN113939588A (zh) 2019-05-15 2022-01-14 诺维信公司 温度敏感性的rna指导的内切核酸酶
CN114207125B (zh) 2019-06-25 2025-01-28 诺维信公司 通过抑制条件性必需基因进行反向选择
US20220340865A1 (en) 2019-07-02 2022-10-27 Basf Se Method for preparing a fermentation medium
BR112023001392A2 (pt) 2020-07-28 2023-02-14 Basf Se Método para produzir uma proteína de interesse a partir de uma célula hospedeira de bacillus
MX2023001272A (es) 2020-07-28 2023-03-03 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de cambio de temperatura.
BR112023001386A2 (pt) 2020-07-28 2023-02-14 Basf Se Método para cultivar uma célula hospedeira de bacillus, e, cultura de células hospedeiras de bacillus
WO2022074056A2 (en) 2020-10-07 2022-04-14 Basf Se Bacillus cell with reduced lipase and/or esterase side activities
WO2022269081A1 (en) 2021-06-24 2022-12-29 Basf Se Bacillus licheniformis host cell for production of a compound of interest with increased purity
CN117693587A (zh) 2021-06-24 2024-03-12 巴斯夫欧洲公司 具有改变的RemA/RemB蛋白的改良芽孢杆菌宿主细胞
EP4370534A1 (en) 2021-07-13 2024-05-22 Novozymes A/S Recombinant cutinase expression
WO2023247514A1 (en) 2022-06-22 2023-12-28 Novozymes A/S Recombinant mannanase expression
US20260062690A1 (en) 2022-08-02 2026-03-05 Basf Se Stabilized protein production process using bacillus host cells
WO2025132815A1 (en) 2023-12-20 2025-06-26 Novozymes A/S Novel cas nucleases and polynucleotides encoding the same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4631257A (en) * 1978-12-26 1986-12-23 Cetus Corporation Stable high copy number plasmids
US4401761A (en) * 1981-01-26 1983-08-30 The Upjohn Company Process for stabilizing plasmids by deletion of DNA

Also Published As

Publication number Publication date
JP3162707B2 (ja) 2001-05-08
EP0506780B2 (en) 2004-01-02
NO922381D0 (no) 1992-06-17
ES2064077T5 (es) 2004-09-01
CA2070676A1 (en) 1991-06-19
AU643741B2 (en) 1993-11-25
NO922381L (no) 1992-08-17
DK0506780T3 (da) 1995-04-18
DE69013520T2 (de) 1995-05-04
DK0506780T4 (da) 2004-04-26
ATE113077T1 (de) 1994-11-15
JPH05502162A (ja) 1993-04-22
FI922812A0 (fi) 1992-06-17
DK639689D0 (da) 1989-12-18
FI922812L (fi) 1992-06-17
DE69013520D1 (de) 1994-11-24
EP0506780B1 (en) 1994-10-19
FI112502B (fi) 2003-12-15
EP0506780A1 (en) 1992-10-07
AU7031291A (en) 1991-07-18
DE69013520T3 (de) 2004-09-23
WO1991009129A1 (en) 1991-06-27

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