ES2064077T3 - Integracion estable de adn en genomas bacterianos. - Google Patents

Integracion estable de adn en genomas bacterianos.

Info

Publication number
ES2064077T3
ES2064077T3 ES91901587T ES91901587T ES2064077T3 ES 2064077 T3 ES2064077 T3 ES 2064077T3 ES 91901587 T ES91901587 T ES 91901587T ES 91901587 T ES91901587 T ES 91901587T ES 2064077 T3 ES2064077 T3 ES 2064077T3
Authority
ES
Spain
Prior art keywords
dna
stable integration
bacterial genomes
genome
reproduction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91901587T
Other languages
English (en)
Other versions
ES2064077T5 (es
Inventor
Steen Troels Jorgensen
Per Lina Jorgensen
Borge Krag Diderichsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8148687&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2064077(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of ES2064077T3 publication Critical patent/ES2064077T3/es
Application granted granted Critical
Publication of ES2064077T5 publication Critical patent/ES2064077T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

INTEGRACION ESTABLE DE ADN EN GENOMAS BACTERIANOS UNA CELULA BACTERIANA EN QUE EN SU GENOMA LLEVA UNA FORMACION DE ADN SIN REPETICION INTEGRADA QUE CONSTA DE (1) UNA SECUENCIA DE ADN DE INTERES, (2) UNA SECUENCIA DE ADN HOMOLOGA A LA REGION DEL GENOMA DE LA CELULA Y (3) UN ORIGEN DE REPRODUCCION, FALTANDOLE A DICHA FORMACION DE ADN UN GEN FUNCIONAL QUE CODIFIQUE EL FACTOR REQUERIDO PARA INICIAR LA REPETICION DE DICHO ORIGEN DE REPRODUCCION.
ES91901587T 1989-12-18 1990-12-18 Integracion estable de adn en genomas bacterianos. Expired - Lifetime ES2064077T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK639689 1989-12-18
DK639689A DK639689D0 (da) 1989-12-18 1989-12-18 Indfoering af dna i celler

Publications (2)

Publication Number Publication Date
ES2064077T3 true ES2064077T3 (es) 1995-01-16
ES2064077T5 ES2064077T5 (es) 2004-09-01

Family

ID=8148687

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91901587T Expired - Lifetime ES2064077T5 (es) 1989-12-18 1990-12-18 Integracion estable de adn en genomas bacterianos.

Country Status (11)

Country Link
EP (1) EP0506780B2 (es)
JP (1) JP3162707B2 (es)
AT (1) ATE113077T1 (es)
AU (1) AU643741B2 (es)
CA (1) CA2070676A1 (es)
DE (1) DE69013520T3 (es)
DK (2) DK639689D0 (es)
ES (1) ES2064077T5 (es)
FI (1) FI112502B (es)
NO (1) NO922381L (es)
WO (1) WO1991009129A1 (es)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2665711B1 (fr) * 1990-08-08 1993-08-13 Centre Nat Rech Scient Integron de corynebacterie, procede de transformation d'une corynebacterie par ledit integron et corynebacterie obtenue.
IL110299A0 (en) 1993-07-15 1994-10-21 Novo Nordisk Entotech Inc Formation of and methods for the production of large bacillus thuringiensis crystals with increased pesticidal activity
AU5001496A (en) * 1995-03-22 1996-10-08 Novo Nordisk A/S Introduction of dna into bacillus strains by conjugation
FR2736066B1 (fr) * 1995-06-30 1998-11-20 Ajinomoto Kk Procede d'amplification d'un gene par transposon artificiel, bacterie coryneforme obtenue par ce procede et procede de production d'un acide amine a l'aide de cette bacterie
EP0878552A1 (en) 1997-05-13 1998-11-18 Erasmus Universiteit Rotterdam Molecular detection of chromosome aberrations
CN100494363C (zh) 1997-12-22 2009-06-03 诺维信公司 糖氧化酶及其在焙烤中的用途
EP1078098A1 (en) 1998-05-04 2001-02-28 Dako A/S Method and probes for the detection of chromosome aberrations
EP2284272B1 (en) 1998-06-10 2014-08-13 Novozymes A/S Mannanases
JP3958884B2 (ja) 1998-09-11 2007-08-15 株式会社林原生物化学研究所 非還元性糖質生成酵素及びトレハロース遊離酵素ならびに該酵素を用いる糖質の製造方法
JP5043254B2 (ja) 1998-10-26 2012-10-10 ノボザイムス アクティーゼルスカブ 糸状面細胞内の問題のｄｎａライブラリーの作製及びスクリーニング
EP1297170B1 (en) 2000-06-23 2016-03-02 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
AU2002311012A1 (en) 2001-06-06 2002-12-16 Novozymes A/S Endo-beta-1,4-glucanase from bacillus
US7521204B2 (en) 2002-04-10 2009-04-21 Novozymes A/S Bacillus host cell
WO2003087149A2 (en) 2002-04-10 2003-10-23 Novozymes A/S Bacillus licheniformis mutant host cell
AU2003223928A1 (en) * 2002-05-07 2003-11-11 Novozymes A/S Homologous recombination into bacterium for the generation of polynucleotide libraries
CN1738899A (zh) 2002-12-11 2006-02-22 诺和酶股份有限公司 洗涤剂组合物
DK1694846T3 (en) 2003-12-10 2014-11-17 Novozymes As Cell with enhanced secretion mediated by MrgA protein or a homolog
WO2005123915A1 (en) 2004-06-21 2005-12-29 Novozymes A/S Stably maintained multiple copies of at least two orf in the same orientation
CN101595214B (zh) 2006-11-29 2013-06-12 诺维信股份有限公司 改进向细菌细胞中导入dna的方法
WO2008140615A2 (en) 2006-12-21 2008-11-20 Novozymes, Inc. Modified messenger rna stabilizing sequences for expressing genes in bacterial cells
CN102762723A (zh) 2009-12-21 2012-10-31 诺维信股份有限公司 用于在巯基-二硫键氧还酶缺陷的细菌突变体细胞中产生异源多肽的方法
EP3532619A1 (en) 2016-10-25 2019-09-04 Novozymes A/S Flp-mediated genomic integration
WO2019016052A1 (en) 2017-07-21 2019-01-24 Basf Se HETEROLOGOUS EXPRESSION PROMOTER
EP3655528A1 (en) 2017-07-21 2020-05-27 Basf Se Method of transforming bacterial cells
EP3707252A1 (en) 2017-11-10 2020-09-16 Novozymes A/S Temperature-sensitive cas9 protein
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
EP3918086A1 (en) 2019-01-30 2021-12-08 Novozymes A/S Cognate foldase co-expression
EP3927837A1 (en) 2019-02-20 2021-12-29 Basf Se Industrial fermentation process for bacillus using defined medium and magnesium feed
EP3927809A1 (en) 2019-02-20 2021-12-29 Basf Se Industrial fermentation process for bacillus using defined medium and trace element feed
US20220170003A1 (en) 2019-03-08 2022-06-02 Novozymes A/S Means And Methods For Improving Protease Expression
WO2020229191A1 (en) 2019-05-15 2020-11-19 Novozymes A/S Temperature-sensitive rna-guided endonuclease
EP3990629A1 (en) 2019-06-25 2022-05-04 Novozymes A/S Counter-selection by inhibition of conditionally essential genes
US20220340865A1 (en) 2019-07-02 2022-10-27 Basf Se Method for preparing a fermentation medium
CN116157530A (zh) 2020-07-28 2023-05-23 巴斯夫欧洲公司 使用温度变化的芽孢杆菌工业发酵工艺
BR112023001386A2 (pt) 2020-07-28 2023-02-14 Basf Se Método para cultivar uma célula hospedeira de bacillus, e, cultura de células hospedeiras de bacillus
BR112023001392A2 (pt) 2020-07-28 2023-02-14 Basf Se Método para produzir uma proteína de interesse a partir de uma célula hospedeira de bacillus
US20240060110A1 (en) 2020-10-07 2024-02-22 Basf Se Bacillus cell with reduced lipase and/or esterase side activities
MX2023015460A (es) 2021-06-24 2024-01-18 Basf Se Celula huesped de bacillus mejorada con proteina rema/remb alterada.
CN117813316A (zh) 2021-06-24 2024-04-02 巴斯夫欧洲公司 用于生产纯度更高的感兴趣的化合物的地衣芽孢杆菌宿主细胞
CN117730090A (zh) 2021-07-13 2024-03-19 诺维信公司 重组角质酶表达
WO2023247514A1 (en) 2022-06-22 2023-12-28 Novozymes A/S Recombinant mannanase expression
EP4565710A1 (en) 2022-08-02 2025-06-11 Basf Se Stabilized protein production process using bacillus host cells
WO2025132815A1 (en) 2023-12-20 2025-06-26 Novozymes A/S Novel cas nucleases and polynucleotides encoding the same
WO2026132030A1 (en) 2024-12-19 2026-06-25 Novozymes A/S Cas nucleases and polynucleotides encoding the same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4631257A (en) * 1978-12-26 1986-12-23 Cetus Corporation Stable high copy number plasmids
US4401761A (en) * 1981-01-26 1983-08-30 The Upjohn Company Process for stabilizing plasmids by deletion of DNA

Also Published As

Publication number Publication date
DK0506780T4 (da) 2004-04-26
FI922812A0 (fi) 1992-06-17
JPH05502162A (ja) 1993-04-22
FI112502B (fi) 2003-12-15
EP0506780A1 (en) 1992-10-07
AU643741B2 (en) 1993-11-25
DK0506780T3 (da) 1995-04-18
NO922381L (no) 1992-08-17
ATE113077T1 (de) 1994-11-15
DK639689D0 (da) 1989-12-18
EP0506780B1 (en) 1994-10-19
DE69013520D1 (de) 1994-11-24
AU7031291A (en) 1991-07-18
EP0506780B2 (en) 2004-01-02
JP3162707B2 (ja) 2001-05-08
ES2064077T5 (es) 2004-09-01
DE69013520T2 (de) 1995-05-04
WO1991009129A1 (en) 1991-06-27
FI922812L (fi) 1992-06-17
CA2070676A1 (en) 1991-06-19
DE69013520T3 (de) 2004-09-23
NO922381D0 (no) 1992-06-17

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