ES2049340T3 - Procedimiento e instalacion para la obtencion de plasmidos y cosmidos. - Google Patents

Procedimiento e instalacion para la obtencion de plasmidos y cosmidos.

Info

Publication number
ES2049340T3
ES2049340T3 ES89401674T ES89401674T ES2049340T3 ES 2049340 T3 ES2049340 T3 ES 2049340T3 ES 89401674 T ES89401674 T ES 89401674T ES 89401674 T ES89401674 T ES 89401674T ES 2049340 T3 ES2049340 T3 ES 2049340T3
Authority
ES
Spain
Prior art keywords
installation
cosmids
treatment chamber
plasmids
obtaining
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89401674T
Other languages
English (en)
Inventor
Daniel Cohen
Frederic Dufau
Jean Hache
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bertin Technologies SAS
Original Assignee
Bertin et Cie SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bertin et Cie SA filed Critical Bertin et Cie SA
Application granted granted Critical
Publication of ES2049340T3 publication Critical patent/ES2049340T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

LA INVENCION SE REFIERE A UN PROCEDIMIENTO Y UNA INSTALACION POR LA OBTENCION DE PLASMIDOS Y COSMIDOS ENTEROS. LA INSTALACION PARA LA OBTENCION DE ESTOS PRESENTES EN CELULAS BACTERIANAS, SEGUN LA INVENCION, COMPRENDE AL MENOS UN MEDIO DE SEPARACION (5) DE LAS CELULAS DE SU MEDIO DE CULTIVO, AL MENOS UN RECINTO DE TRATAMIENTO (4), AL MENOS UN GEL DE ELECTROFORESIS (7) QUE SIRVE DE MEDIO DE MIGRACION ELECTROFONETICA, ADYACENTE Y ASOCIADA A DICHO RECINTO DE TRATAMIENTO, AL MENOS UN MEDIO DE APLICACION DE UN CAMPO ELECTRICO (8) PROVOCANDO LA MIGRACION ELECTROFONETICA DE LOS MEDIOS AMOVIBLES Y/O ECLIPSABLES (II) DE PUESTA EN COMUNICACION DE DICHO GEL DE ELECTROFORESIS CON DICHO RECINTO DE TRATAMIENTO Y UN MICROPROCESADOR DE AUTOMATIZACION PARA EL PEDIDO SECUENCIAL DE DICHO MEDIO DE SEPARACION, DE DICHOS MEDIOS DE PUESTA EN COMUNICACION Y DEL MEDIO DE APLICACION DEL CAMPO ELECTRICO.
ES89401674T 1988-06-21 1989-06-15 Procedimiento e instalacion para la obtencion de plasmidos y cosmidos. Expired - Lifetime ES2049340T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8808306A FR2632970B1 (fr) 1988-06-21 1988-06-21 Procede et installation pour l'obtention de plasmides et de cosmides

Publications (1)

Publication Number Publication Date
ES2049340T3 true ES2049340T3 (es) 1994-04-16

Family

ID=9367529

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89401674T Expired - Lifetime ES2049340T3 (es) 1988-06-21 1989-06-15 Procedimiento e instalacion para la obtencion de plasmidos y cosmidos.

Country Status (9)

Country Link
EP (1) EP0348275B1 (es)
JP (1) JPH03501210A (es)
AT (1) ATE101645T1 (es)
AU (1) AU3843689A (es)
DE (1) DE68913087T2 (es)
DK (1) DK45790A (es)
ES (1) ES2049340T3 (es)
FR (1) FR2632970B1 (es)
WO (1) WO1989012682A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6362003B1 (en) 1992-02-24 2002-03-26 Coulter Corporation Hematological reference control composition containing leukocyte analogs, methods of making, and uses thereof
FR2742163B1 (fr) * 1995-12-06 1998-03-20 Agronomique Inst Nat Rech Procede d'identification d'especes bacteriennes

Also Published As

Publication number Publication date
DE68913087T2 (de) 1994-05-26
DK45790D0 (da) 1990-02-21
WO1989012682A1 (fr) 1989-12-28
JPH03501210A (ja) 1991-03-22
EP0348275A1 (fr) 1989-12-27
FR2632970A1 (fr) 1989-12-22
DE68913087D1 (de) 1994-03-24
EP0348275B1 (fr) 1994-02-16
ATE101645T1 (de) 1994-03-15
DK45790A (da) 1990-02-21
FR2632970B1 (fr) 1990-09-28
AU3843689A (en) 1990-01-12

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