ES2049701T1 - Procedimiento de fabricacion de fibrinogeno con una pureza muy elevada. - Google Patents
Procedimiento de fabricacion de fibrinogeno con una pureza muy elevada.Info
- Publication number
- ES2049701T1 ES2049701T1 ES93400247T ES93400247T ES2049701T1 ES 2049701 T1 ES2049701 T1 ES 2049701T1 ES 93400247 T ES93400247 T ES 93400247T ES 93400247 T ES93400247 T ES 93400247T ES 2049701 T1 ES2049701 T1 ES 2049701T1
- Authority
- ES
- Spain
- Prior art keywords
- fibrinogen
- solution
- ionic strength
- high purity
- agarose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000008946 Fibrinogen Human genes 0.000 title abstract 5
- 108010049003 Fibrinogen Proteins 0.000 title abstract 5
- 229940012952 fibrinogen Drugs 0.000 title abstract 5
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- FBPFZTCFMRRESA-ZXXMMSQZSA-N D-iditol Chemical compound OC[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-ZXXMMSQZSA-N 0.000 title 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 4
- 239000000243 solution Substances 0.000 abstract 4
- 229920000936 Agarose Polymers 0.000 abstract 3
- 238000005349 anion exchange Methods 0.000 abstract 2
- 239000011324 bead Substances 0.000 abstract 2
- 239000011780 sodium chloride Substances 0.000 abstract 2
- 125000003161 (C1-C6) alkylene group Chemical group 0.000 abstract 1
- 239000007983 Tris buffer Substances 0.000 abstract 1
- 125000003277 amino group Chemical group 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 210000002381 plasma Anatomy 0.000 abstract 1
- 125000006850 spacer group Chemical group 0.000 abstract 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/75—Fibrinogen
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
- Compounds Of Unknown Constitution (AREA)
Abstract
LA INVENCION SE REFIERE A UN PROCESO DE FABRICACION DE FIBRINOGENO DE ALTA PUREZA. ESTE PROCESO SE CARACTERIZA EN QUE COMPRENDE, A PARTIR DE CRIOPRECIPITADO DE PLASMA SANGUINEO DE ORIGEN ANIMAL O HUMANO, PUESTO EN SOLUCION ACUOSA, EL TRATAMIENTO DE ESTA SOLUCION PARA LLEVARLA A UNA FUERZA IONICA IGUAL A LA DE UNA SOLUCION TAMPON QUE TIENE LA SIGUIENTE COMPOSICION: NACL 120MM TRIS 20 MM PH 8,8 SE HACE PASAR ESTA SOLUCION DE FUERZA IONICA AJUSTA SOBRE UNA COLUMNA INTERCAMBIADORA DE ANION QUE COMPRENDE UN GEL DE INTERCAMBIO DE ANION, POR EJEMPLO EN FORMA DE BOLAS, A BASE DE AGAROSA RETICULADO QUE COMPRENDE GRUPOS AMINO CUATERNARIO PARTICULARMENTE EN EL EXTREMO DE UN PEQUEÑO BRAZO SEPARADO, POR EJEMPLO DE TIPO ALQUILENO EN C1-C6 CONECTADO A LAS BOLAS DE AGAROSA, EN PARTICULAR A APROXIMADAMENTE 6% DE AGAROSA Y SE PROCEDE A UNA ELUCION CON UNA PRIMERA SOLUCION DE ELUCION DE IGUAL FUERZA IONICA, PERMITIENDO ESTAS CONDICIONES ABSORBER AL MENOS SELECTIVAMENTE EL FIBRINOGENO SOBRE DICHA COLUMNA, QUEESTA DESORBIDO POR UNA SOLUCION DE ELUCION DE FUERZA IONICA MAS ELEVADA A 200 MM NACL. SE OBTIENE UN FIBRINOGENO DE ALTA PUREZA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9201238A FR2686883B1 (fr) | 1992-02-04 | 1992-02-04 | Procede de fabrication de fibrinogene de tres haute purete, fibrinogene de tres haute purete obtenu ainsi qu'une composition pharmaceutique le contenant. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2049701T1 true ES2049701T1 (es) | 1994-05-01 |
| ES2049701T3 ES2049701T3 (es) | 1997-01-16 |
Family
ID=9426316
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES93400247T Expired - Lifetime ES2049701T3 (es) | 1992-02-04 | 1993-02-02 | Procedimiento de fabricacion de fibrinogeno de pureza muy elevada. |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0555135B1 (es) |
| AT (1) | ATE141284T1 (es) |
| DE (2) | DE69303941T2 (es) |
| DK (1) | DK0555135T3 (es) |
| ES (1) | ES2049701T3 (es) |
| FR (1) | FR2686883B1 (es) |
| GR (2) | GR930300095T1 (es) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0602173B1 (en) * | 1991-09-05 | 1999-05-26 | Baxter International Inc. | Topical fibrinogen complex |
| DE69512416T3 (de) * | 1994-07-14 | 2011-12-15 | Croix Rouge De Belgique | Fibrinogenkonzentrate aus blutplasma, verfahren und anlage für ihre herstellung |
| DE10261126A1 (de) * | 2002-08-13 | 2004-03-04 | Aventis Behring Gmbh | Lagerungsstabile, flüssige Fibrinogen-Formulierung |
| DE102004009400A1 (de) | 2004-02-24 | 2005-09-08 | Zlb Behring Gmbh | Fibrinogen Reinigung |
| FR2887883B1 (fr) | 2005-06-29 | 2007-08-31 | Lab Francais Du Fractionnement | Procede de separation des proteines fibrinogene, facteur xiii et colle biologique d'une fraction plasmatique solubilisee et de preparation de concentres lyophilises desdites proteines |
| HUE028626T2 (en) | 2008-06-23 | 2016-12-28 | Bio-Products & Bio-Engineering Ag | Stable, functionally intact, virus-inactivated fibrinogen during storage |
| EP2619225B1 (en) * | 2010-09-20 | 2016-06-15 | Octapharma AG | Process for production of fibrinogen |
| US10112972B2 (en) | 2012-03-13 | 2018-10-30 | Octapharma Ag | Process for production of fibrinogen and fibrinogen produced thereby |
| US20140154233A1 (en) | 2012-12-05 | 2014-06-05 | Csl Limited | Method of purifying therapeutic proteins |
| US10188965B2 (en) | 2012-12-05 | 2019-01-29 | Csl Behring Gmbh | Hydrophobic charge induction chromatographic depletion of a protein from a solution |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2632309B1 (fr) * | 1988-06-07 | 1990-08-24 | Lille Transfusion Sanguine | Procede de purification par voie chromatographique de proteines, notamment de facteur viii, et les produits obtenus |
| FR2644064B1 (fr) * | 1989-02-17 | 1994-05-27 | Aquitaine Dev Transf Sanguine | Procede de fabrication du facteur antihemophilique fviiic ayant une tres haute purete et facteur antihemophilique fviiic ainsi obtenu, ainsi que composition pharmaceutique le contenant |
| IT1248723B (it) * | 1990-06-12 | 1995-01-26 | Scalvo S P A | Processo per la purificazione del fattore viii e fattore viii ottenuto con tale processo |
-
1992
- 1992-02-04 FR FR9201238A patent/FR2686883B1/fr not_active Expired - Fee Related
-
1993
- 1993-02-02 AT AT93400247T patent/ATE141284T1/de not_active IP Right Cessation
- 1993-02-02 ES ES93400247T patent/ES2049701T3/es not_active Expired - Lifetime
- 1993-02-02 DK DK93400247.8T patent/DK0555135T3/da active
- 1993-02-02 DE DE69303941T patent/DE69303941T2/de not_active Expired - Fee Related
- 1993-02-02 EP EP93400247A patent/EP0555135B1/fr not_active Expired - Lifetime
- 1993-02-02 DE DE93400247T patent/DE555135T1/de active Pending
- 1993-09-30 GR GR930300095T patent/GR930300095T1/el unknown
-
1996
- 1996-10-23 GR GR960402791T patent/GR3021419T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE69303941T2 (de) | 1997-03-27 |
| EP0555135A1 (fr) | 1993-08-11 |
| DE555135T1 (de) | 1994-03-31 |
| FR2686883B1 (fr) | 1994-05-13 |
| ATE141284T1 (de) | 1996-08-15 |
| DE69303941D1 (de) | 1996-09-19 |
| EP0555135B1 (fr) | 1996-08-14 |
| GR930300095T1 (en) | 1993-09-30 |
| GR3021419T3 (en) | 1997-01-31 |
| ES2049701T3 (es) | 1997-01-16 |
| DK0555135T3 (da) | 1996-11-25 |
| FR2686883A1 (fr) | 1993-08-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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