ES2053766T3 - Metodo para aumentar la expresion de un gen. - Google Patents

Metodo para aumentar la expresion de un gen.

Info

Publication number
ES2053766T3
ES2053766T3 ES88310665T ES88310665T ES2053766T3 ES 2053766 T3 ES2053766 T3 ES 2053766T3 ES 88310665 T ES88310665 T ES 88310665T ES 88310665 T ES88310665 T ES 88310665T ES 2053766 T3 ES2053766 T3 ES 2053766T3
Authority
ES
Spain
Prior art keywords
yeast
protein
respiratory
gen
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88310665T
Other languages
English (en)
Inventor
Kazuo Nakahama
Yoshihiko Kaisho
Koji Yoshimura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takeda Pharmaceutical Co Ltd
Original Assignee
Takeda Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Chemical Industries Ltd filed Critical Takeda Chemical Industries Ltd
Application granted granted Critical
Publication of ES2053766T3 publication Critical patent/ES2053766T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/94—Saccharomyces
    • Y10S435/942—Saccharomyces cerevisiae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

SE DESCUBREN LEVADURAS DEFICIENTES RESPIRATORIAS EXCEPTO SACCHAROMYCES CEREVISIAL AH22R-, SIENDO TRANSFORMASA DICHA LEVADURA DEFIECIENTE RESPIRATORIA CON UN ADN CONTENIENDO UN GEN QUE CODIFICA UNA PROTEINA EXTRAÑA LEVADURA Y UN METODO DE PREPARACION DE UNA PROTEINA EXTRAÑA LEVADURA DESCRITA MAS ARRIBA, QUE COMPRENDE EL CULTIVO DE LA LEVADURA Y PRODUCCION Y ACUMULACION DE LA PROTEINA EN UN CULTIVO. LA LEVADURA DEFICIENTE RESPIRATORIA DE LA PRESENTE INVENCION PUEDE PRODUCIR UNA CANTIDAD SUPERIOR DE PROTEINA QUE LA DE LA CEPA PADRE.
ES88310665T 1987-11-13 1988-11-11 Metodo para aumentar la expresion de un gen. Expired - Lifetime ES2053766T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP28517587 1987-11-13

Publications (1)

Publication Number Publication Date
ES2053766T3 true ES2053766T3 (es) 1994-08-01

Family

ID=17688070

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88310665T Expired - Lifetime ES2053766T3 (es) 1987-11-13 1988-11-11 Metodo para aumentar la expresion de un gen.

Country Status (6)

Country Link
US (1) US5182195A (es)
EP (1) EP0317209B1 (es)
AT (1) ATE85644T1 (es)
CA (1) CA1312029C (es)
DE (1) DE3878410T2 (es)
ES (1) ES2053766T3 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0358325A1 (en) * 1988-08-10 1990-03-14 Takeda Chemical Industries, Ltd. Production of protein kinase C by yeast
HU214698B (hu) * 1992-04-09 1998-06-29 Gyógyszerkutató Intézet Kft. Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására
US5342763A (en) * 1992-11-23 1994-08-30 Genentech, Inc. Method for producing polypeptide via bacterial fermentation
US5891669A (en) * 1997-03-17 1999-04-06 Novo Nordisk A/S, Novoalle, Methods for producing polypeptides in respiratory-deficient cells
WO1999049028A1 (en) 1998-03-23 1999-09-30 Genentech, Inc. Method of selection for genes encoding secreted and transmembrane proteins
JPWO2004022742A1 (ja) * 2002-09-05 2005-12-22 株式会社イーエムアイ 生理活性物質のスクリーニング方法
FR2857977B1 (fr) * 2003-07-23 2007-10-05 Centre Nat Rech Scient Procede de production industrielle d'arn et systeme utile pour ladite production

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4752576A (en) * 1984-06-14 1988-06-21 Chiron Corporation Expression of α-1 antitrypsin in yeast
US4766068A (en) * 1984-06-16 1988-08-23 Agency Of Industrial Science And Technology Cytochrome P-450MC gene, expression plasmid carrying the said gene, yeasts transformed with the said plasmid and a process for producing cytochrome P-450MC by culturing the said transformant yeasts
EP0171908A3 (en) * 1984-07-11 1987-07-15 Takeda Chemical Industries, Ltd. Hepatitis b virus surface antigen and production thereof
JPS6170989A (ja) * 1984-09-13 1986-04-11 Takeda Chem Ind Ltd 組み換えｄｎａおよびその用途
CA1263619A (en) * 1984-10-09 1989-12-05 Ryuji Marumoto Dna, production and use thereof
EP0181634A3 (en) * 1984-11-14 1987-09-09 Takeda Chemical Industries, Ltd. Synthetic gene for human lysozyme
JPH0745514B2 (ja) * 1985-08-20 1995-05-17 武田薬品工業株式会社 新規蛋白質およびその製造法
WO1987003298A1 (en) * 1985-11-21 1987-06-04 The Australian National University Method for genetically marking yeast
TW205070B (es) * 1986-06-30 1993-05-01 Takeda Pharm Industry Co Ltd

Also Published As

Publication number Publication date
EP0317209A2 (en) 1989-05-24
ATE85644T1 (de) 1993-02-15
US5182195A (en) 1993-01-26
EP0317209A3 (en) 1989-10-18
CA1312029C (en) 1992-12-29
EP0317209B1 (en) 1993-02-10
DE3878410D1 (de) 1993-03-25
DE3878410T2 (de) 1993-06-03

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