ES2054129T3 - Procedimiento para la expresion de un gen recombinante. - Google Patents

Procedimiento para la expresion de un gen recombinante.

Info

Publication number
ES2054129T3
ES2054129T3 ES90105447T ES90105447T ES2054129T3 ES 2054129 T3 ES2054129 T3 ES 2054129T3 ES 90105447 T ES90105447 T ES 90105447T ES 90105447 T ES90105447 T ES 90105447T ES 2054129 T3 ES2054129 T3 ES 2054129T3
Authority
ES
Spain
Prior art keywords
expression
procedure
recombinant gene
agg
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90105447T
Other languages
English (en)
Inventor
Ulrich Dipl-Biologe Brinkmann
Ralf Prof Dr Rer Nat Mattes
Anne Dr Rer Nat Stern
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2054129T3 publication Critical patent/ES2054129T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

EL INVENTO SE REFIERE A LA EXPRESION DE UNOS GENES RECOMBINANTES EN E COLI A TRAVES DE LA TRANSFORMACION CON VECTOR DE EXPRESION QUE CONTIENE EL GEN RECOMBINANTE PARA REDUCIR EN MAS DE UN 3% EN CORDEONES AGA- Y/O AGG DE GENES RECOMBINANTES RESPECTO A LA CANTIDAD TOTAL DE CORDONES EN EL GEN. LOS GENES CONTIENEN CORDONES AGG Y/O AGG PARA ARGININA.
ES90105447T 1989-03-23 1990-03-22 Procedimiento para la expresion de un gen recombinante. Expired - Lifetime ES2054129T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3909710A DE3909710A1 (de) 1989-03-23 1989-03-23 Verfahren zur expression eines rekombinanten gens

Publications (1)

Publication Number Publication Date
ES2054129T3 true ES2054129T3 (es) 1994-08-01

Family

ID=6377123

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90105447T Expired - Lifetime ES2054129T3 (es) 1989-03-23 1990-03-22 Procedimiento para la expresion de un gen recombinante.

Country Status (8)

Country Link
EP (1) EP0388963B1 (es)
JP (1) JPH07102146B2 (es)
AT (1) ATE106099T1 (es)
CA (1) CA2012632C (es)
DE (2) DE3909710A1 (es)
DK (1) DK0388963T3 (es)
ES (1) ES2054129T3 (es)
IL (1) IL93851A (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
EP2275559A3 (en) * 1999-09-28 2011-03-23 Shire Human Genetic Therapies, Inc. Optimized messenger RNA
AU2001249170A1 (en) * 2000-03-13 2001-09-24 Aptagen Method for modifying a nucleic acid
AU2001286211A1 (en) 2000-09-14 2002-03-26 Takara Bio Inc Thermotolerant ribonuclease h
TWI247804B (en) 2000-09-26 2006-01-21 Takara Bio Inc Sphingolipid ceramide deacylase gene
KR20070032737A (ko) 2004-06-04 2007-03-22 다카라 바이오 가부시키가이샤 Ｄｎａ 폴리메라아제 활성을 갖는 폴리펩티드
EP2215231A4 (en) * 2007-10-15 2010-12-01 Univ Queensland EXPRESSION SYSTEM FOR MODULATING AN IMMUNE RESPONSE

Also Published As

Publication number Publication date
CA2012632C (en) 1998-07-28
EP0388963B1 (de) 1994-05-25
DE3909710A1 (de) 1990-09-27
JPH02286086A (ja) 1990-11-26
IL93851A0 (en) 1990-12-23
JPH07102146B2 (ja) 1995-11-08
CA2012632A1 (en) 1990-09-23
DE59005790D1 (de) 1994-06-30
DK0388963T3 (da) 1994-08-29
EP0388963A1 (de) 1990-09-26
ATE106099T1 (de) 1994-06-15
IL93851A (en) 1999-01-26

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