ES2054342T3 - Metodo de separar oligonucleotidos de una mezcla. - Google Patents

Metodo de separar oligonucleotidos de una mezcla.

Info

Publication number
ES2054342T3
ES2054342T3 ES90903639T ES90903639T ES2054342T3 ES 2054342 T3 ES2054342 T3 ES 2054342T3 ES 90903639 T ES90903639 T ES 90903639T ES 90903639 T ES90903639 T ES 90903639T ES 2054342 T3 ES2054342 T3 ES 2054342T3
Authority
ES
Spain
Prior art keywords
indicator
sequence
oligonucleotide
nucleotide
complementary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90903639T
Other languages
English (en)
Inventor
Sudhir Agrawal
Paul C Zamecnik
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Worcester Foundation for Biomedical Research
Original Assignee
Worcester Foundation for Biomedical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Worcester Foundation for Biomedical Research filed Critical Worcester Foundation for Biomedical Research
Application granted granted Critical
Publication of ES2054342T3 publication Critical patent/ES2054342T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

METODO PARA LA DEPURACION ABSOLUTA DE INDICADORES SINTETICOS OLIGONUCLEOTIDOS DE UNA AMALGAMA DE LOS MISMOS PARTICULARMENTE DE UNA MEZCLA CON SECUENCIA TRUNCADA O DISMINUIDA. EL METODO COMPRENDE LA CONEXION DE UNA SECUENCIA COMPLEMENTARIA ABREVIADA AL TERMINAL DE UN OLIGONUCLEOTIDO 5'' INDICADOR, A UN APOYO SOLIDO. EL NEXO COMPLEMENTARIO ENTRE LA MAYORIA DE NUCLEOTIDOS 5'' DE INDICADOR Y EL NUCLEOTIDO DESLIGADO QUE RESULTA DE LA HIBRIDACION, SIRVE PARA RETENER EL NUCLEOTIDO INDICADOR. TRUNCADAS O DISMINUIDAS LAS SECUENCIAS CARENTES DE 5'', Y SECUENCIAS COMPLEMENTARIAS DEL OLIGONUCLEOTIDO AGREGADO, FALLAN EN LA HIBRIDACION Y POR OTRA PARTE NO SON RETENIDAS. EL METODO POSIBILITA LA PURIFICACION DE CANTIDADES GRAM DE ACIDOS DEOXYRIBONUCLEICOS SINTETICOS Y SECUENCIAS QUE CONTIENEN MODIFICACIONES TALES COMO EL FOSFATO EN LA COLUMNA VERTEBRAL. LA SECUENCIA DE SOPORTE DE NUCLEOTIDO AISLADO, ES ESTABLE EN CONDICIONES DE DEPURACION PUDIENDO POR ELLO, SER REUTILIZADAS.
ES90903639T 1989-02-15 1990-02-15 Metodo de separar oligonucleotidos de una mezcla. Expired - Lifetime ES2054342T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/311,111 US5352578A (en) 1989-02-15 1989-02-15 Method of separating oligonucleotides from a mixture
CA002049750A CA2049750A1 (en) 1989-02-15 1991-08-23 Method of separating oligonucleotides from a mixture

Publications (1)

Publication Number Publication Date
ES2054342T3 true ES2054342T3 (es) 1994-08-01

Family

ID=25674746

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90903639T Expired - Lifetime ES2054342T3 (es) 1989-02-15 1990-02-15 Metodo de separar oligonucleotidos de una mezcla.

Country Status (5)

Country Link
US (1) US5352578A (es)
EP (1) EP0458876B1 (es)
CA (1) CA2049750A1 (es)
ES (1) ES2054342T3 (es)
WO (1) WO1990009393A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4239531A1 (de) * 1992-11-25 1994-05-26 Gabor Dr Igloi Reagenz zur Kupplung verschiedener Substanzen an Nukleinsäuren sowie Verfahren zu dessen Herstellung
US7038026B2 (en) 2000-05-26 2006-05-02 Centelion Purification of a triple heli formation with an immobilized oligonucleotide
FR2728264B1 (fr) 1994-12-16 1997-01-31 Rhone Poulenc Rorer Sa Purification d'adn par formation de triple helice avec un oligonucleotide immobilise
US6599704B2 (en) * 1997-03-04 2003-07-29 Christoph Wagener Method for detecting mutated alleles
DE19708758A1 (de) * 1997-03-04 1998-09-17 Christoph Prof Dr Wagener Verfahren zum Nachweis von mutierten Allelen
WO2001055451A1 (en) * 2000-01-28 2001-08-02 Linden Technologies, Inc. C-3' protected monomeric nucleotides and synthesis of oligonucleotides on solid support
CA2445838C (en) * 2001-04-30 2011-11-22 Avecia Biotechnology Inc Immobilization of oligonucleotides onto solid supports
US7279280B2 (en) * 2003-09-25 2007-10-09 Mgp Biotech, Inc. Apparatus and method for detecting genetic mutations and single nucleotide polymorphisms

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4358586A (en) * 1980-12-23 1982-11-09 Harvey Rubin Detection and isolation of endorphin mRNA using a synthetic oligodeoxynucleotide
EP0067597A1 (en) * 1981-05-29 1982-12-22 ens BIO LOGICALS INC. Polynucleotide synthesis procedure
WO1985001051A1 (en) * 1983-09-02 1985-03-14 Molecular Biosystems, Inc. Oligonucleotide polymeric support system
US4818681A (en) * 1985-02-22 1989-04-04 Molecular Diagnostics, Inc. Fast and specific immobilization of nucleic acids to solid supports
US4806546A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on derivatized nylon supports

Also Published As

Publication number Publication date
US5352578A (en) 1994-10-04
EP0458876B1 (en) 1993-04-07
EP0458876A1 (en) 1991-12-04
WO1990009393A1 (en) 1990-08-23
CA2049750A1 (en) 1993-02-24

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