ES2055031T3 - Ensayo de enzima de preincubacion de receptor. - Google Patents
Ensayo de enzima de preincubacion de receptor.Info
- Publication number
- ES2055031T3 ES2055031T3 ES89300928T ES89300928T ES2055031T3 ES 2055031 T3 ES2055031 T3 ES 2055031T3 ES 89300928 T ES89300928 T ES 89300928T ES 89300928 T ES89300928 T ES 89300928T ES 2055031 T3 ES2055031 T3 ES 2055031T3
- Authority
- ES
- Spain
- Prior art keywords
- analyte
- sample
- enzyme
- beta
- amount
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000001952 enzyme assay Methods 0.000 title 1
- 238000011533 pre-incubation Methods 0.000 title 1
- 239000012491 analyte Substances 0.000 abstract 9
- 238000000034 method Methods 0.000 abstract 5
- 102000005936 beta-Galactosidase Human genes 0.000 abstract 3
- 108010005774 beta-Galactosidase Proteins 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 abstract 1
- 238000006555 catalytic reaction Methods 0.000 abstract 1
- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 abstract 1
- 229960005156 digoxin Drugs 0.000 abstract 1
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 abstract 1
- 238000003018 immunoassay Methods 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
SE PROPORCIONA UN METODO DE INMUNOENSAYO DE COMPLEMENTACION DE (BETA)-GALACTOSIDASA PARA DETERMINAR LA CONCENTRACION DE UN ANALITO EN UNA MUESTRA. EL METODO SE BASA EN COMBINAR UN COMPLEJO CONJUGADO DE ANALITO DONADOR DE ENZIMA PREFORMADO (ED)-ANTICUERPO ANTIANALITO CON EL ANALITO PRESENTE EN LA MUESTRA. EL METODO CONSISTE EN HACER REACCIONAR UN COMPLEJO DE CONJUGADO DE ANALITO-ED (BETA)-GALACTOSIDASA ANTICUERPO ANTIANALITO, UN ACEPTOR DE ENZIMA (BETA)-GALACTOSIDASA (EA), SUBSTRATO DE ENZIMA Y MUESTRA, Y DETERMINAR LA VELOCIDAD DE REACCION CATALIZADA POR ENZIMA COMO INDICATIVA DE LA CANTIDAD DE ANALITO PRESENTE EN LA MUESTRA. NORMALMENTE, SE AÑADE EL EA A LA MEZCLA DE REACCION DESPUES DE UN TIEMPO PARA QUE EL ANTICUERPO DEL ANTIANALITO DEL COMPLEJO REACCIONE CON EL ANALITO PRESENTE EN LA MUESTRA. EN UNA INCORPORACION PARTICULAR, EL METODO SE UTILIZA PARA DETERMINAR LA CANTIDAD DE DIGOXIMA EN UNA MUESTRA DE SUERO. EN OTRA INCORPORACION, EL METODO SE UTILIZA PARA DETERMINAR LA CANTIDAD DET3 EN UNA MUESTRA. TAMBIEN SE PROPORCIONAN EQUIPOS PARA FACILITAR EL METODO.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15141288A | 1988-02-02 | 1988-02-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2055031T3 true ES2055031T3 (es) | 1994-08-16 |
Family
ID=22538651
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES89300928T Expired - Lifetime ES2055031T3 (es) | 1988-02-02 | 1989-01-31 | Ensayo de enzima de preincubacion de receptor. |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0327312B1 (es) |
| JP (1) | JPH01296162A (es) |
| AT (1) | ATE104058T1 (es) |
| AU (1) | AU612775B2 (es) |
| DE (1) | DE68914305T2 (es) |
| ES (1) | ES2055031T3 (es) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5604091A (en) * | 1984-03-01 | 1997-02-18 | Microgenics Corporation | Methods for protein binding enzyme complementation |
| CA1335707C (en) * | 1989-08-15 | 1995-05-30 | Pyare Khanna | Drug screening assay |
| EP0419081A3 (en) * | 1989-09-22 | 1992-07-22 | Microgenics Corporation | Method for protein binding enzyme complementation assays |
| WO1996019732A1 (en) * | 1994-12-19 | 1996-06-27 | Microgenics Corporation | Competitive binding assays having improved linearity |
| RU2132553C1 (ru) * | 1996-05-30 | 1999-06-27 | Ивановская Елена Алексеевна | Количественное определение дигоксина в сыворотке крови методом инверсионной вольтамперометрии |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3100062A1 (de) * | 1981-01-02 | 1982-08-05 | Hans A. Dipl.-Chem. Dr. 8000 München Thoma | Homogenes verfahren zur bestimmung von liganden |
| US4708929A (en) * | 1984-10-29 | 1987-11-24 | Microgenics Corporation | Methods for protein binding enzyme complementation assays |
| EP0199801B1 (en) * | 1984-10-29 | 1993-08-25 | MICROGENICS CORPORATION (a Delaware corporation) | Methods for protein binding enzyme complementation assays |
-
1989
- 1989-01-31 EP EP89300928A patent/EP0327312B1/en not_active Expired - Lifetime
- 1989-01-31 DE DE68914305T patent/DE68914305T2/de not_active Expired - Fee Related
- 1989-01-31 ES ES89300928T patent/ES2055031T3/es not_active Expired - Lifetime
- 1989-01-31 AT AT89300928T patent/ATE104058T1/de not_active IP Right Cessation
- 1989-02-01 AU AU28988/89A patent/AU612775B2/en not_active Ceased
- 1989-02-02 JP JP1022727A patent/JPH01296162A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP0327312A3 (en) | 1990-08-22 |
| EP0327312A2 (en) | 1989-08-09 |
| AU2898889A (en) | 1989-08-03 |
| AU612775B2 (en) | 1991-07-18 |
| DE68914305D1 (de) | 1994-05-11 |
| EP0327312B1 (en) | 1994-04-06 |
| ATE104058T1 (de) | 1994-04-15 |
| DE68914305T2 (de) | 1994-10-06 |
| JPH01296162A (ja) | 1989-11-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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