ES2058250T3 - Un metodo para regular la expresion de un gen extraño controlando la concentracion de azucar en un medio y un procedimiento para producir por ello un producto de gen extraño. - Google Patents

Un metodo para regular la expresion de un gen extraño controlando la concentracion de azucar en un medio y un procedimiento para producir por ello un producto de gen extraño.

Info

Publication number
ES2058250T3
ES2058250T3 ES88301355T ES88301355T ES2058250T3 ES 2058250 T3 ES2058250 T3 ES 2058250T3 ES 88301355 T ES88301355 T ES 88301355T ES 88301355 T ES88301355 T ES 88301355T ES 2058250 T3 ES2058250 T3 ES 2058250T3
Authority
ES
Spain
Prior art keywords
expression
gene
strange
concentration
sugar
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88301355T
Other languages
English (en)
Inventor
Nobuhiro Fukuhara
Setsuo Yoshino
Satori Sone
Yoshiyuki Nakajima
Nobuyoshi Makiguchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsui Toatsu Chemicals Inc
Original Assignee
Mitsui Toatsu Chemicals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP62034396A external-priority patent/JPS63202385A/ja
Priority claimed from JP62152358A external-priority patent/JPH0817703B2/ja
Application filed by Mitsui Toatsu Chemicals Inc filed Critical Mitsui Toatsu Chemicals Inc
Application granted granted Critical
Publication of ES2058250T3 publication Critical patent/ES2058250T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

ESCHERISCHIA COLI, QUE PORTA UN PLASMIDO HIBRIDO QUE HA SIDO CONSTRUIDO POR INSERCION DE UN GEN EXTRAÑO DESEADO EN UN VECTOR DE EXPRESION, DE MANERA QUE PERMITE LA EXPRESION DE DICHO GEN EN EL, SE CULTIVA EN UN MEDIO QUE CONTIENE UN COMPONENTE DE AZUCAR UTILIZABLE POR E. COLI COMO FUENTE DE CARBONO A UNA CONCENTRACION DE 0,3 % O SUPERIOR, DE MODO QUE LA EXPRESION DE DICHO GEN EXTRAÑO DESEADO SE DETIENE. ESTE E. COLI (POR EJEMPLO, TRANSFORMANTE) SE CULTIVA EN EL MEDIO EN EL QUE LA CONCENTRACION DE AZUCAR SE MANTIENE A 0,3 % O MAS EN UN PRIMER PROCESO, DE MODO QUE SE DETIENE LA SUPRESION DEL GEN EXTRAÑO Y SE MANTIENE SUFICIENTE CRECIMIENTO CELULAR, Y DESPUES MENOS QUE 0,3 % EN UN SEGUNDO PROCESO, PARA QUITAR LA SUPRESION DE LA EXPRESION, DE MODO QUE PERMITE LA PRODUCCION EFECTIVA DEL PRODUCTO DE GEN EXTRAÑO, LO QUE CONDUCE A UNA ALTA CONCENTRACION DE PRODUCTO CON GEN EXTRAÑO EN EL CULTIVO FINAL.
ES88301355T 1987-02-19 1988-02-18 Un metodo para regular la expresion de un gen extraño controlando la concentracion de azucar en un medio y un procedimiento para producir por ello un producto de gen extraño. Expired - Lifetime ES2058250T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP62034396A JPS63202385A (ja) 1987-02-19 1987-02-19 外来遺伝子の発現制御方法及び該方法を用いた外来遺伝子産物の生産方法
JP62152358A JPH0817703B2 (ja) 1987-06-18 1987-06-18 外来遺伝子産物の生産方法

Publications (1)

Publication Number Publication Date
ES2058250T3 true ES2058250T3 (es) 1994-11-01

Family

ID=26373201

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88301355T Expired - Lifetime ES2058250T3 (es) 1987-02-19 1988-02-18 Un metodo para regular la expresion de un gen extraño controlando la concentracion de azucar en un medio y un procedimiento para producir por ello un producto de gen extraño.

Country Status (7)

Country Link
EP (1) EP0279664B1 (es)
KR (1) KR910001811B1 (es)
CA (1) CA1319630C (es)
DE (1) DE3882247T2 (es)
DK (1) DK88588A (es)
ES (1) ES2058250T3 (es)
MX (1) MX168450B (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4946790A (en) * 1987-02-06 1990-08-07 Mitsui Toatsu Chemicals, Inc. Recombinant plasmid for the expression of L-phenylalanine ammonia-lyase and transformed strain carrying same
US5279951A (en) * 1989-05-08 1994-01-18 Research Association For Utilization Of Light Oil Cultivation of transformed microorganisms
DE3925550A1 (de) * 1989-08-02 1991-02-07 Behringwerke Ag Optimierte fermentationsverfahren zur herstellung von fremdproteinen in e.coli
GB8924883D0 (en) * 1989-11-03 1989-12-20 Shell Int Research Expression vector and polypeptide synthesis

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60221091A (ja) * 1983-12-21 1985-11-05 Kyowa Hakko Kogyo Co Ltd 新規プロモ−タ−
GB8621626D0 (en) * 1986-09-08 1986-10-15 Health Lab Service Board Phenylalanine ammonia lyase
JP2507345B2 (ja) * 1986-09-16 1996-06-12 三井東圧化学株式会社 Ｌ―フェニルアラニン―アンモニアリ・ア―ゼ構造遺伝子

Also Published As

Publication number Publication date
MX168450B (es) 1993-05-25
KR910001811B1 (ko) 1991-03-26
EP0279664A3 (en) 1989-06-14
EP0279664A2 (en) 1988-08-24
KR880010118A (ko) 1988-10-07
DK88588D0 (da) 1988-02-19
DK88588A (da) 1988-08-20
CA1319630C (en) 1993-06-29
DE3882247T2 (de) 1993-10-28
EP0279664B1 (en) 1993-07-14
DE3882247D1 (de) 1993-08-19

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