ES2058271T3 - Metodo amperometrico para la determinacion cuantitativa de 1,4-dihidronicotinamida-adenina en solucion. - Google Patents

Metodo amperometrico para la determinacion cuantitativa de 1,4-dihidronicotinamida-adenina en solucion.

Info

Publication number
ES2058271T3
ES2058271T3 ES88303921T ES88303921T ES2058271T3 ES 2058271 T3 ES2058271 T3 ES 2058271T3 ES 88303921 T ES88303921 T ES 88303921T ES 88303921 T ES88303921 T ES 88303921T ES 2058271 T3 ES2058271 T3 ES 2058271T3
Authority
ES
Spain
Prior art keywords
solution
carbon electrode
quantitative determination
dihydronicotinamide
adenine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88303921T
Other languages
English (en)
Inventor
Hugh Peter Bennetto
Gerard Michael Delaney
Jeremy Richard Mason
Christopher Frank Thurston
John Laing Stirling
David Robert Dekeyzer
William Henry Mullen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cambridge Life Sciences PLC
Original Assignee
Cambridge Life Sciences PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge Life Sciences PLC filed Critical Cambridge Life Sciences PLC
Application granted granted Critical
Publication of ES2058271T3 publication Critical patent/ES2058271T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/40—Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/817—Enzyme or microbe electrode

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Electrodes For Compound Or Non-Metal Manufacture (AREA)

Abstract

EL METODO PARA LA DETERMINACION CUANTITATIVA DE NADH EN SOLUCION, IMPLICA PONER EN CONTACTO LA SOLUCION QUE CONTIENE NADH CON UN ELECTRODO DE CARBONO ACTIVADO, MANTENER EL ELECTRODO DE ARBONO A UN POTENCIAL FIJO CONTROLADO, EFECTIVO PARA CAUSAR OXIDACION DEL NADH EN LA SUPERFICE DEL ELECTRODO Y MEDIR LA CORRIENTE QUE SALE DEL ELECTRODO DE CARBONO, EN EL QUE SE USA UN ELECTRODO DE CARBONO ACTIVADO QUE CONTIENE UN METAL NOBLE, PREFERIBLEMENTE PLATINIZADO O PALADIZADO, QUE COMPRENDE UNA CAPA POROSA, HETEROGENEA, AGLOMERADA CON RESINA, DE PARTICULAS DE CARBONO O GRAFITO ACTIVADOS QUE CONTIENE EL METAL NOBLE FINAMENTE DIVIDIDO PREADSORBIDO SOBRE ELLA, Y AGLOMERADA CON UNA RESINA NATURAL O SINTETICA, PREFERIBLEMENTE CON UNA RESINA HIDROFOBA COMO EL POLITETRAFLUORETILENO.
ES88303921T 1987-05-01 1988-04-29 Metodo amperometrico para la determinacion cuantitativa de 1,4-dihidronicotinamida-adenina en solucion. Expired - Lifetime ES2058271T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB878710472A GB8710472D0 (en) 1987-05-01 1987-05-01 Amperometric method

Publications (1)

Publication Number Publication Date
ES2058271T3 true ES2058271T3 (es) 1994-11-01

Family

ID=10616757

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88303921T Expired - Lifetime ES2058271T3 (es) 1987-05-01 1988-04-29 Metodo amperometrico para la determinacion cuantitativa de 1,4-dihidronicotinamida-adenina en solucion.

Country Status (18)

Country Link
US (1) US5122456A (es)
EP (2) EP0289344B1 (es)
JP (2) JPH01503409A (es)
KR (1) KR960001501B1 (es)
AU (2) AU1686188A (es)
CA (1) CA1304127C (es)
DE (1) DE3882078T2 (es)
DK (1) DK169559B1 (es)
ES (1) ES2058271T3 (es)
FI (1) FI92221C (es)
GB (3) GB8710472D0 (es)
HU (1) HU205625B (es)
IE (1) IE60238B1 (es)
IL (2) IL86222A0 (es)
MX (1) MX167769B (es)
NO (1) NO179953C (es)
RU (1) RU1806187C (es)
WO (2) WO1988008447A1 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8710472D0 (en) * 1987-05-01 1987-06-03 Cambridge Life Sciences Amperometric method
GB8817997D0 (en) * 1988-07-28 1988-09-01 Cambridge Life Sciences Enzyme electrodes & improvements in manufacture thereof
DE3932247A1 (de) * 1989-09-27 1991-04-04 Biotechnolog Forschung Gmbh Elektrodenmaterial, elektroden, verfahren zur herstellung und verwendung der elektrode
TW279133B (es) * 1990-12-13 1996-06-21 Elan Med Tech
AT397513B (de) * 1992-12-15 1994-04-25 Avl Verbrennungskraft Messtech Amperometrische enzymelektrode
US5429735A (en) * 1994-06-27 1995-07-04 Miles Inc. Method of making and amperometric electrodes
IE72524B1 (en) * 1994-11-04 1997-04-23 Elan Med Tech Analyte-controlled liquid delivery device and analyte monitor
DE4442253A1 (de) * 1994-11-28 1996-05-30 Bayer Corp N D Ges D Staates I Elektrochemischer Enzymbiosensor
US6413410B1 (en) 1996-06-19 2002-07-02 Lifescan, Inc. Electrochemical cell
FR2743575B1 (fr) * 1996-01-17 1998-03-13 Univ Toulouse Procede perfectionne de modification en surface d'une electrode pour la realisation d'un reaction electrochimique et electrodes obtenues
WO1998020331A1 (en) * 1996-11-07 1998-05-14 Cambridge Sensors Limited Electrodes and their use in assays
CA2294610A1 (en) 1997-06-16 1998-12-23 George Moshe Katz Methods of calibrating and testing a sensor for in vivo measurement of an analyte and devices for use in such methods
KR100491316B1 (ko) * 2002-05-29 2005-05-24 삼성엔지니어링 주식회사 망간이 고정된 흑연 또는 뉴트랄 레드가 결합된 흑연을포함하는 ｎａｄ(ｐ)+ 또는 ｎａｄ(ｐ)ｈ의 직접 환원또는 산화용 전극
ES2441361T3 (es) * 2007-09-18 2014-02-04 Ultizyme International Ltd. Electrodo enzimático
JP5362406B2 (ja) * 2009-03-25 2013-12-11 三洋電機株式会社 燃料電池
JP5930810B2 (ja) 2011-04-26 2016-06-08 アークレイ株式会社 分析用具
EP2731169B1 (en) * 2011-07-07 2017-01-04 Toyota Jidosha Kabushiki Kaisha Secondary battery
US11819038B2 (en) 2019-01-18 2023-11-21 Frieslandcampina Nederland B.V. Shaped cheese product
CN116698945A (zh) * 2023-06-09 2023-09-05 深圳津合生物有限公司 一种酶电极系统及其应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4044193A (en) * 1971-06-16 1977-08-23 Prototech Company Finely particulated colloidal platinum compound and sol for producing the same, and method of preparation of fuel cell electrodes and the like employing the same
SU593439A1 (ru) * 1975-08-04 1980-10-07 Ордена Трудового Красного Знамени Институт Химических Наук Ан Казахской Сср Способ получени электропроводных фермент-ковакторных систем
US4166143A (en) * 1977-01-24 1979-08-28 Prototech Company Control of the interaction of novel platinum-on-carbon electrocatalysts with fluorinated hydrocarbon resins in the preparation of fuel cell electrodes
US4321123A (en) * 1978-04-21 1982-03-23 Matsushita Electric Industrial Co., Ltd. Coenzyme immobilized electrode
JPS5816695B2 (ja) * 1978-04-24 1983-04-01 松下電器産業株式会社 酵素電極
JPS584982B2 (ja) * 1978-10-31 1983-01-28 松下電器産業株式会社 酵素電極
US4293396A (en) * 1979-09-27 1981-10-06 Prototech Company Thin carbon-cloth-based electrocatalytic gas diffusion electrodes, and electrochemical cells comprising the same
US4478696A (en) * 1982-07-21 1984-10-23 Prototech Company Ionizable reducing and oxidizing gaseous supply means and process for catalytic barriers and electrodes
GB8606824D0 (en) * 1986-03-19 1986-04-23 Univ Strathclyde Biochemical detector
GB8612861D0 (en) * 1986-05-27 1986-07-02 Cambridge Life Sciences Immobilised enzyme biosensors
GB8710472D0 (en) * 1987-05-01 1987-06-03 Cambridge Life Sciences Amperometric method

Also Published As

Publication number Publication date
US5122456A (en) 1992-06-16
GB2204698B (en) 1991-04-17
GB8810180D0 (en) 1988-06-02
CA1304127C (en) 1992-06-23
HUT50863A (en) 1990-03-28
MX167769B (es) 1993-04-12
IE881274L (en) 1988-11-01
DK731988A (da) 1989-02-28
JP2528956B2 (ja) 1996-08-28
DK169559B1 (da) 1994-11-28
JPH01503410A (ja) 1989-11-16
RU1806187C (ru) 1993-03-30
NO179953C (no) 1997-01-15
IL86223A (en) 1993-01-14
NO179953B (no) 1996-10-07
JPH01503409A (ja) 1989-11-16
GB2204415B (en) 1991-05-08
GB8810181D0 (en) 1988-06-02
GB8710472D0 (en) 1987-06-03
FI92221B (fi) 1994-06-30
IL86223A0 (en) 1988-11-15
DK731988D0 (da) 1988-12-30
AU1686188A (en) 1988-12-02
IL86222A0 (en) 1988-11-15
NO885836L (no) 1988-12-30
KR960001501B1 (ko) 1996-01-31
FI92221C (fi) 1994-10-10
DE3882078T2 (de) 1994-01-20
FI886050A7 (fi) 1988-12-30
AU1686288A (en) 1988-12-02
KR890701721A (ko) 1989-12-21
IE60238B1 (en) 1994-06-15
AU612366B2 (en) 1991-07-11
EP0289344A1 (en) 1988-11-02
NO885836D0 (no) 1988-12-30
DE3882078D1 (de) 1993-08-05
GB2204698A (en) 1988-11-16
WO1988008446A1 (en) 1988-11-03
HU205625B (en) 1992-05-28
WO1988008447A1 (en) 1988-11-03
EP0289344B1 (en) 1993-06-30
EP0289345A1 (en) 1988-11-02
GB2204415A (en) 1988-11-09

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