ES2065915T3 - Procedimiento para aumentar la expresion de polipeptidos en un cultivo de celula recombinante. - Google Patents

Procedimiento para aumentar la expresion de polipeptidos en un cultivo de celula recombinante.

Info

Publication number
ES2065915T3
ES2065915T3 ES88308387T ES88308387T ES2065915T3 ES 2065915 T3 ES2065915 T3 ES 2065915T3 ES 88308387 T ES88308387 T ES 88308387T ES 88308387 T ES88308387 T ES 88308387T ES 2065915 T3 ES2065915 T3 ES 2065915T3
Authority
ES
Spain
Prior art keywords
cell
expression
polypeptides
procedure
increase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88308387T
Other languages
English (en)
Inventor
Arthur D Levinson
Jennie P Mather
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ES2065915T3 publication Critical patent/ES2065915T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Oncology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PROPORCIONES DE CELULA VIABLES Y DENSIDADES DE CELULAS TOTALES EN CULTIVOS DE CELULAS EUCAROTIDAS SON INCREMENTADAS POR TRANSFECCION DE UNA CELULA HOSPEDADORA EUCAROTIDA CON ACIDO NUCLEICO CODIFICANDO UN POLIPEPTIDO DESEADO Y CON UN ONCOGENE. LOS CULTIVOS TRANSFERIDOS SON ANALIZADOS PARA LA EXPRESION DE CANTIDADES ELEVADAS DEL POLIPEPTIDO DESEADO EN COMPARACION CON LA EXPRESION DE LA CELULA HOSPEDADORA PADRE.
ES88308387T 1987-09-11 1988-09-12 Procedimiento para aumentar la expresion de polipeptidos en un cultivo de celula recombinante. Expired - Lifetime ES2065915T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US9724687A 1987-09-11 1987-09-11

Publications (1)

Publication Number Publication Date
ES2065915T3 true ES2065915T3 (es) 1995-03-01

Family

ID=22262433

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88308387T Expired - Lifetime ES2065915T3 (es) 1987-09-11 1988-09-12 Procedimiento para aumentar la expresion de polipeptidos en un cultivo de celula recombinante.

Country Status (8)

Country Link
EP (1) EP0307248B1 (es)
JP (1) JP2796547B2 (es)
AT (1) ATE113984T1 (es)
AU (1) AU612404B2 (es)
CA (1) CA1312566C (es)
DE (1) DE3852082T2 (es)
ES (1) ES2065915T3 (es)
IE (1) IE64979B1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL87737A (en) * 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
US5256553A (en) * 1987-10-09 1993-10-26 Immunex Corporation Multiple promoter transforming retroviral vectors
US5648254A (en) * 1988-01-15 1997-07-15 Zymogenetics, Inc. Co-expression in eukaryotic cells
DE68929419T2 (de) * 1988-12-13 2003-04-03 Colorado State University Research Foundation, Fort Collins Prototypische FeLV-Isolate für die Verwendung als Krankheitsmuster oder Impfstoff
FR2650838A1 (fr) * 1989-08-09 1991-02-15 Transgene Sa Vecteurs d'expression du facteur ix dans une cellule eucaryote superieure, procede de preparation de facteur ix par des animaux transgeniques et facteur ix obtenu
GB8928884D0 (en) * 1989-12-21 1990-02-28 Feldmann Marc Transformed cell lines
WO1991014781A1 (en) * 1990-03-19 1991-10-03 Henkel Research Corporation METHOD FOR INCREASING THE OMEGA-HYDROXYLASE ACTIVITY IN $i(CANDIDA TROPICALIS)
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
AUPN442295A0 (en) * 1995-07-26 1995-08-17 Commonwealth Scientific And Industrial Research Organisation Regulated autocrine growth of mammalian cells

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT85828B (pt) * 1986-09-30 1990-08-31 Smithkline Beecham Corp Profcesso de producao de uma linha de ceculas de mamifero, recombinante, linha de celulas assim obtida e processo de preparacao de um gene
ES2038602T3 (es) * 1986-10-15 1993-08-01 Battelle Memorial Institute Metodo para la produccion de proteinas por medio de sistemas de expresion inducible en celulas-huesped en carioticas geneticamente modificadas multiplicadas in-vivo.

Also Published As

Publication number Publication date
JP2796547B2 (ja) 1998-09-10
EP0307248A3 (en) 1990-04-25
IE882769L (en) 1989-03-11
DE3852082D1 (de) 1994-12-15
EP0307248B1 (en) 1994-11-09
AU2209988A (en) 1989-04-20
ATE113984T1 (de) 1994-11-15
AU612404B2 (en) 1991-07-11
EP0307248A2 (en) 1989-03-15
DE3852082T2 (de) 1995-05-04
CA1312566C (en) 1993-01-12
JPH01120300A (ja) 1989-05-12
IE64979B1 (en) 1995-09-20

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