ATE113984T1 - Verfahren zur erhöhung der expression von polypeptiden in rekombinanten zellkulturen. - Google Patents

Verfahren zur erhöhung der expression von polypeptiden in rekombinanten zellkulturen.

Info

Publication number: ATE113984T1
Authority: AT; Austria
Prior art keywords: expression; polypeptides; increasing; cell cultures; recombinant cell
Prior art date: 1987-09-11

Application number

AT88308387T

Other languages

English (en)

Inventor

Arthur D Levinson

Jennie P Mather

Original Assignee

Genentech Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-09-11

Filing date

1988-09-12

Publication date

1994-11-15

1988-09-12 Application filed by Genentech Inc filed Critical Genentech Inc

1994-11-15 Application granted granted Critical

1994-11-15 Publication of ATE113984T1 publication Critical patent/ATE113984T1/de

Links

229920001184 polypeptide Polymers 0.000 title abstract 3
102000004196 processed proteins & peptides Human genes 0.000 title abstract 3
108090000765 processed proteins & peptides Proteins 0.000 title abstract 3
238000004113 cell culture Methods 0.000 title abstract 2
238000000034 method Methods 0.000 title 1
210000004027 cell Anatomy 0.000 abstract 4
108700020796 Oncogene Proteins 0.000 abstract 1
210000003527 eukaryotic cell Anatomy 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/82—Translation products from oncogenes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Medicinal Chemistry (AREA)
Microbiology (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Physics & Mathematics (AREA)
Endocrinology (AREA)
Toxicology (AREA)
Diabetes (AREA)
Oncology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)

AT88308387T 1987-09-11 1988-09-12 Verfahren zur erhöhung der expression von polypeptiden in rekombinanten zellkulturen. ATE113984T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US9724687A	1987-09-11	1987-09-11

Publications (1)

Publication Number	Publication Date
ATE113984T1 true ATE113984T1 (de)	1994-11-15

Family

ID=22262433

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT88308387T ATE113984T1 (de)	1987-09-11	1988-09-12	Verfahren zur erhöhung der expression von polypeptiden in rekombinanten zellkulturen.

Country Status (8)

Country	Link
EP (1)	EP0307248B1 (de)
JP (1)	JP2796547B2 (de)
AT (1)	ATE113984T1 (de)
AU (1)	AU612404B2 (de)
CA (1)	CA1312566C (de)
DE (1)	DE3852082T2 (de)
ES (1)	ES2065915T3 (de)
IE (1)	IE64979B1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IL87737A (en) *	1987-09-11	1993-08-18	Genentech Inc	Method for culturing polypeptide factor dependent vertebrate recombinant cells
US5256553A (en) *	1987-10-09	1993-10-26	Immunex Corporation	Multiple promoter transforming retroviral vectors
US5648254A (en) *	1988-01-15	1997-07-15	Zymogenetics, Inc.	Co-expression in eukaryotic cells
ES2179854T3 (es) *	1988-12-13	2003-02-01	Harvard College	Materiales aislados de felv prototipos para uso en modelos de enfermedades y vacunas.
FR2650838A1 (fr) *	1989-08-09	1991-02-15	Transgene Sa	Vecteurs d'expression du facteur ix dans une cellule eucaryote superieure, procede de preparation de facteur ix par des animaux transgeniques et facteur ix obtenu
GB8928884D0 (en) *	1989-12-21	1990-02-28	Feldmann Marc	Transformed cell lines
WO1991014781A1 (en) *	1990-03-19	1991-10-03	Henkel Research Corporation	METHOD FOR INCREASING THE OMEGA-HYDROXYLASE ACTIVITY IN $i(CANDIDA TROPICALIS)
GB9022545D0 (en)	1990-10-17	1990-11-28	Wellcome Found	Culture medium
AUPN442295A0 (en) *	1995-07-26	1995-08-17	Commonwealth Scientific And Industrial Research Organisation	Regulated autocrine growth of mammalian cells

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0262942A1 (de) *	1986-09-30	1988-04-06	Smithkline Beecham Corporation	Zelltransfektion
EP0263908B1 (de) *	1986-10-15	1991-09-25	Battelle Memorial Institute	Verfahren zur Herstellung von Proteinen mittels induzierbarer Expressionssysteme in in-vivo-vermehrten, genetisch modifizierten eukaryotischen Wirtszellen

1988
- 1988-09-09 JP JP63227338A patent/JP2796547B2/ja not_active Expired - Lifetime
- 1988-09-09 AU AU22099/88A patent/AU612404B2/en not_active Expired
- 1988-09-12 DE DE3852082T patent/DE3852082T2/de not_active Expired - Lifetime
- 1988-09-12 EP EP88308387A patent/EP0307248B1/de not_active Expired - Lifetime
- 1988-09-12 CA CA577153A patent/CA1312566C/en not_active Expired - Lifetime
- 1988-09-12 ES ES88308387T patent/ES2065915T3/es not_active Expired - Lifetime
- 1988-09-12 IE IE276988A patent/IE64979B1/en not_active IP Right Cessation
- 1988-09-12 AT AT88308387T patent/ATE113984T1/de not_active IP Right Cessation

Also Published As

Publication number	Publication date
IE64979B1 (en)	1995-09-20
EP0307248B1 (de)	1994-11-09
EP0307248A3 (en)	1990-04-25
ES2065915T3 (es)	1995-03-01
JPH01120300A (ja)	1989-05-12
AU612404B2 (en)	1991-07-11
IE882769L (en)	1989-03-11
DE3852082D1 (de)	1994-12-15
JP2796547B2 (ja)	1998-09-10
EP0307248A2 (de)	1989-03-15
CA1312566C (en)	1993-01-12
DE3852082T2 (de)	1995-05-04
AU2209988A (en)	1989-04-20

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Legal Events

Date	Code	Title	Description
2008-12-15	EELA	Cancelled due to lapse of time